LOCUS       pMC-1-CcdB-11           2892 bp ds-DNA     circular     04-NOV-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    90..93
                     /label="J1"
                     /ApEinfo_revcolor=#ff0080
                     /ApEinfo_fwdcolor=#ff0080
     misc_feature    146..726
                     /label="CcdB negative selection cassette"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    199..384
                     /label="CcdA (2bp insertion mutant)"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    421..726
                     /label="CcdB"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    735..738
                     /label="J11"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    860..964
                     /label="Bacterial promoter"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             965..1975
                     /label="SpecR marker: spectinomycin/streptomycin adenyltransferase"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2127..2892
                     /label="pBR322 ORI fragment"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2146..2734
                     /label="pBR322 ORI"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcag gagttgtctt caagccagat aacagtatgc
      121 gtatttgcgc gctgattttt gcggtataag aatatatact gatatgtata cccgaagtat
      181 gtcaaaagga ggtatgctat gaagcagcgt attacagtga cagttgacag cgacagctat
      241 cagttgctca aggcatatat gatgtcaata tctccggtct ggtaagcaca accatgcaga
      301 atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa gggatggctg
      361 aggtcgcccg gtttattgaa atgaacggct cttttgctga cgagaacagg ggctggtgaa
      421 atgcagttta aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta
      481 cagagtgata ttattgacac gcccgggcga cggatggtga tccccctggc cagtgcacgt
      541 ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc
      601 tggcgcatga tgaccaccga tatggccagt gtgccggttt ccgttatcgg ggaagaagtg
      661 gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga
      721 atataagaag acaacgcttg agaccacgaa gtggctcttc agtggacgaa agggcctcgt
      781 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
      841 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
      901 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
      961 gagtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc
     1021 agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc
     1081 atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga
     1141 tgttacgcag cagggcagtc gccctaaaac aaagttaaac atcatgaggg aagcggtgat
     1201 cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc
     1261 gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag
     1321 cgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt
     1381 gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt
     1441 agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga
     1501 actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac
     1561 gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt
     1621 aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct
     1681 aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt
     1741 agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga
     1801 tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga
     1861 agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt
     1921 ggaagaattt gtccattacg taaaaggcga gatcaccaag gtagtcggca aataactgtc
     1981 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     2041 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     2101 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     2161 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     2221 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     2281 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     2341 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     2401 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     2461 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     2521 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     2581 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     2641 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     2701 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     2761 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     2821 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     2881 cgagcgcagc ga
//