LOCUS       pJMC-4-BsaI             2053 bp ds-DNA     circular     23-MAY-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    41..44
                     /label="M4"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    41..78
                     /label="Module 4 Linker: BsaI"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    45..48
                     /label="M1"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    71..74
                     /label="M omega"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    75..78
                     /label="M5"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    135..239
                     /label="Bacterial promoter"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    240..1100
                     /label="AmpR marker: class A beta-lactamase"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1288..2053
                     /label="pMB1-derived ORI fragment"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1307..1895
                     /label="pMB1-derived ORI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc acctgcTTTT CCCAACCGtG AGACCaacaa
       61 caaGGTCTCt AGTGCCTCTT TTgcaggtgT Gatgaaaagc cttagggtca ggtggcactt
      121 ttcggggaaa tgtgcgatga acccctattt gtttattttt ctaaatacat tcaaatatgt
      181 atccgctaat gagacaataa ccctgataaa tgcttcaatt atattgaaaa aggaagagta
      241 tgagtatcca acatttccgt gtcgccctta ttcccttttt tgcggccttc tgccttcctg
      301 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgccc
      361 gagtgggtta cattgaactg gatctcaaca gcggcaaaat cttagagagt tttcgccccg
      421 aagaacgctt cccaatgatg agcactttca aagttctgct atgtggcgcg gtgttatccc
      481 gtattgatgc tgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
      541 ttgagtattc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
      601 gtagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgattg
      661 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
      721 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
      781 ctgtagcaat ggcaacaact ctccgcaaac tattaactgg cgaactactt actcttgctt
      841 cacgccagca actcattgac tggatggagg cggacaaagt tgcaggacca cttctgcgct
      901 cggcacttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtggCtctc
      961 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     1021 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     1081 cactgattaa gcattggtaa ctgtcagacg aagggaaata aatagtagcc cgcctgatgt
     1141 gcgggctttt ttttggtggt ggttaccagt gtcgcttagc taccgtaggt cctcttaggc
     1201 ggtccgatta gcgacatagt catcaatgcc ttgacggcaa aatcccttaa cgtgagtttt
     1261 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     1321 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     1381 tgccggatca agagctacta actctttttc cgaaggtaac tggcttcagc agagcgcaga
     1441 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     1501 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     1561 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     1621 gctgaacggg gggttcgtgc tcacagccca gcttggagcg aacgacctac accgaactga
     1681 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     1741 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     1801 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     1861 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     1921 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     1981 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     2041 ccgagcgcag cga
//