LOCUS       pJMC-5T-BsaI            2065 bp ds-DNA     circular     23-MAY-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    41..44
                     /label="M5"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    41..74
                     /label="Module 5T Linker: BsaI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    45..48
                     /label="M1"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    71..74
                     /label="M omega"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    131..251
                     /label="Bacterial promoter"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    218..233
                     /label="duplication"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    252..1112
                     /label="AmpR marker: class A beta-lactamase"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1300..2065
                     /label="pMB1-derived ORI fragment"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1319..1907
                     /label="pMB1-derived ORI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc acctgcTTTT CCTCACCGtG AGACCaacaa
       61 caaGGTCTCt AGTGTTTTgc aggtgTGatg aaaagcctta gggtcaggtg gcacttttcg
      121 gggaaatgtg cgatgaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
      181 gctaatgaga caataaccct gataaatgct tcaattaTAT GAAAAAGGAA GAGtattgaa
      241 aaaggaagag tatgagtatc caacatttcc gtgtcgccct tattcccttt tttgcggcct
      301 tctgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      361 agttgggtgc ccgagtgggt tacattgaac tggatctcaa cagcggcaaa atcttagaga
      421 gttttcgccc cgaagaacgc ttcccaatga tgagcacttt caaagttctg ctatgtggcg
      481 cggtgttatc ccgtattgat gctgggcaag agcaactcgg tcgccgcata cactattctc
      541 agaatgactt ggttgagtat tcaccagtca cagaaaagca tcttacggat ggcatgacag
      601 taagagaatt atgtagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      661 tgacaacgat tggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      721 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      781 acaccacgat gcctgtagca atggcaacaa ctctccgcaa actattaact ggcgaactac
      841 ttactcttgc ttcacgccag caactcattg actggatgga ggcggacaaa gttgcaggac
      901 cacttctgcg ctcggcactt ccggctggct ggtttattgc tgataaatct ggagccggtg
      961 agcgtggCtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     1021 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     1081 agataggtgc ctcactgatt aagcattggt aactgtcaga cgaagggaaa taaatagtag
     1141 cccgcctgat gtgcgggctt ttttttggtg gtggttacca gtgtcgctta gctaccgtag
     1201 gtcctcttag gcggtccgat tagcgacata gtcatcaatg ccttgacggc aaaatccctt
     1261 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     1321 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     1381 cggtggtttg tttgccggat caagagctac taactctttt tccgaaggta actggcttca
     1441 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     1501 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     1561 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     1621 cgcagcggtc gggctgaacg gggggttcgt gctcacagcc cagcttggag cgaacgacct
     1681 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     1741 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     1801 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     1861 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     1921 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     1981 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     2041 gcagccgaac gaccgagcgc agcga
//