LOCUS       pJMC-4T-Esp3I           2049 bp ds-DNA     circular     23-MAY-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    41..44
                     /label="M4"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    41..74
                     /label="Module 4T Linker: Esp3I"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    45..48
                     /label="M1"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    71..74
                     /label="M omega"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    131..235
                     /label="Bacterial promoter"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    236..1096
                     /label="AmpR marker: class A beta-lactamase"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1284..2049
                     /label="pMB1-derived ORI fragment"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1303..1891
                     /label="pMB1-derived ORI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc acctgcTTTT CCCAACCGtG AGACGaacaa
       61 caaCGTCTCt AGTGTTTTgc aggtgTGatg aaaagcctta gggtcaggtg gcacttttcg
      121 gggaaatgtg cgatgaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
      181 gctaatgaga caataaccct gataaatgct tcaattatat tgaaaaagga agagtatgag
      241 tatccaacat ttccgtgtcg cccttattcc cttttttgcg gccttctgcc ttcctgtttt
      301 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcccgagt
      361 gggttacatt gaactggatc tcaacagcgg caaaatctta gagagttttc gccccgaaga
      421 acgcttccca atgatgagca ctttcaaagt tctgctatgt ggcgcggtgt tatcccgtat
      481 tgatgctggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
      541 gtattcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgtag
      601 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgattggagg
      661 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
      721 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
      781 agcaatggca acaactctcc gcaaactatt aactggcgaa ctacttactc ttgcttcacg
      841 ccagcaactc attgactgga tggaggcgga caaagttgca ggaccacttc tgcgctcggc
      901 acttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg gCtctcgcgg
      961 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     1021 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     1081 gattaagcat tggtaactgt cagacgaagg gaaataaata gtagcccgcc tgatgtgcgg
     1141 gctttttttt ggtggtggtt accagtgtcg cttagctacc gtaggtcctc ttaggcggtc
     1201 cgattagcga catagtcatc aatgccttga cggcaaaatc ccttaacgtg agttttcgtt
     1261 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     1321 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     1381 ggatcaagag ctactaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     1441 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     1501 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     1561 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     1621 aacggggggt tcgtgctcac agcccagctt ggagcgaacg acctacaccg aactgagata
     1681 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     1741 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     1801 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     1861 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     1921 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     1981 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     2041 gcgcagcga
//