LOCUS       pJMC-L2-Donor-Esp3I     4429 bp ds-DNA     circular     30-APR-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter        169..199
                     /label="lac UV5 promoter"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    252..629
                     /label="Eco47I_fragment1"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    632..2088
                     /label="L2 restoration block"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    639..642
                     /label="M1"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    654..1441
                     /label="Chloramphenicol selection cassette"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    677..707
                     /label="Lac UV5 promoter"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    761..1441
                     /label="Chloramphenicol acetyltransferase"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1486..2066
                     /label="CcdB selection cassette"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    1539..1724
                     /label="CcdA (2bp insertion mutant)"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    1761..2066
                     /label="CcdB"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    2078..2081
                     /label="M omega"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    2089..2444
                     /label="Eco47I_fragment2"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     promoter        2547..2651
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#008080
                     /ApEinfo_fwdcolor=#008080
     misc_feature    2652..3512
                     /label="AmpR: class A beta-lactamase"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    complement(3664..4429)
                     /label="pUC19 origin"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    3683..4271
                     /label="pMB1 ori"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcaa ttggcagtga
      121 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
      181 gcttccggct cgtataatgt gtggaattat gagcggataa taatttcaca caggaggttt
      241 aaactttaaa catgtcaaaa gagacAtctt ttgttaagaa tgctgaggaa cttgcaaagc
      301 aaaaaatgga tgctattaac cctgaacttt cttcaaaatt taaattttta ataaaattcc
      361 tgtctcagtt tcctgaagct tgctctaaac ctcgttcaaa aaaaatgcag aataaagttg
      421 gtcaagagga acatattgaa tatttagctc gtagttttca tgagagtcga ttgccaagaa
      481 aacccacgcc acctacaacg gttcctgatg aggtggttag catagttctt aatataagtt
      541 ttaatataca gcctgaaaat cttgagagaa taaaagaaga acatcgattt tccatggcag
      601 ctgagaatat tgtaggagat cttctagaat tCGTCTCtAC CGttttgcag gtgcggccgc
      661 attaggcacc ccaggcttta cactttatgc ttccggctcg tataatgtgt ggattttgag
      721 ttaggatccg tcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg
      781 atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc
      841 agtcgctcaa tgtacctata accagaccgt tcagctTgat attacggcct ttttaaagac
      901 cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat
      961 gaatgctcat ccggaattcc gtatggcaat gaaagacggt gagctggtga tatgggatag
     1021 tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag
     1081 tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta
     1141 cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtTtcagc
     1201 caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt
     1261 cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct
     1321 ggcgattcag gttcatcatg ccgtttgtga tggcttccat gtcggcagaa tgcttaatga
     1381 attacaacag tactgcgatg agtggcaggc ggggcgtaat ctagaggatc cggcttacta
     1441 aaagccagat aacagtatgc gtatttgcgc gctgattttt gcggtataag aatatatact
     1501 gatatgtata cccgaagtat gtcaaaagga ggtatgctat gaagcagcgt attacagtga
     1561 cagttgacag cgacagctat cagttgctca aggcatatat gatgtcaata tctccggtct
     1621 ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga
     1681 aaatcaggaa gggatggctg aggtcgcccg gtttattgaa atgaacggct cttttgctga
     1741 cgagaacagg ggctggtgaa atgcagttta aggtttacac ctataaaaga gagagccgtt
     1801 atcgtctgtt tgtggatgta cagagtgata ttattgacac gcccgggcga cggatggtga
     1861 tccccctggc cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg
     1921 tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt gtgccggttt
     1981 ccgttatcgg ggaagaagtg gctgatctca gccaccgcga aaatgacatc aaaaacgcca
     2041 ttaacctgat gttctgggga atataaCACC TGCAAAAAGT GaGAGACGat cttgctgaaa
     2101 aactcgagcc atccggaaga tctggcggcc gctctcccta tagtgagtcg tattacgccg
     2161 gatggatatg gtgttcaggc acaagtgtta aagcagttga ttttattcac tatgatgaaa
     2221 aaaacaatga atggaacctg ctccaagtta aaaatagaga taataccgaa aactcatcga
     2281 gtagtaagat tagagataat acaacaataa aaaaatggtt tagaacttac tcacagcgtg
     2341 atgctactaa ttgggacaat tttccagatg aagtatcatc taagaattta aatgaagaag
     2401 acttcagagc ttttgttaaa aattatttgg caaaaataat ataattcggc tgcaggggcg
     2461 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     2521 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     2581 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     2641 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     2701 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     2761 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     2821 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     2881 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     2941 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     3001 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     3061 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     3121 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     3181 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     3241 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     3301 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     3361 agcgtgggtc Acgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     3421 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     3481 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     3541 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     3601 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     3661 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     3721 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     3781 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     3841 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     3901 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     3961 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     4021 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     4081 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     4141 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     4201 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     4261 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     4321 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     4381 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcga
//