LOCUS       pTij_sg_mmu_Med6        9760 bp DNA     circular SYN 27-JAN-2023
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 27, 2023 from SnapGene 6.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9760
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     misc_feature    1..249
                     /label=U6_promoter
                     /note="color: #a6acb3"
     misc_feature    251..270
                     /label=mmu_Med6 tracer RNA
                     /note="color: #ff00ff"
     misc_feature    271..352
                     /label=chimeric_tracrRNA
                     /note="color: #a6acb3"
     misc_feature    443..1241
                     /label=CBh_prom
                     /note="color: #a6acb3"
     misc_feature    1374..5474
                     /label=Cas9_CDS
                     /note="color: #a6acb3"
     misc_feature    6346..6854
                     /label=PGK
                     /note="color: #a6acb3"
     misc_feature    6877..7596
                     /label=Venus
                     /note="color: #a6acb3"
     misc_feature    8080..8937
                     /label=AmpR
                     /note="color: #a6acb3"
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      241 cgaaacaccg actccagtga atgttggacg gttttagagc tagaaatagc aagttaaaat
      301 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttgttttaga
      361 gctagaaata gcaagttaaa ataaggctag tccgttttta gcgcgtgcgc caattctgca
      421 gacaaatggc tctagaggta cccgttacat aacttacggt aaatggcccg cctggctgac
      481 cgcccaacga cccccgccca ttgacgtcaa tagtaacgcc aatagggact ttccattgac
      541 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      601 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattgtgccc
      661 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      721 ttaccatggt cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccctccc
      781 cacccccaat tttgtattta tttatttttt aattattttg tgcagcgatg ggggcggggg
      841 gggggggggg gcgcgcgcca ggcggggcgg ggcggggcga ggggcggggc ggggcgaggc
      901 ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct tttatggcga
      961 ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga gtcgctgcga
     1021 cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga
     1081 ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat
     1141 tagctgagca agaggtaagg gtttaaggga tggttggttg gtggggtatt aatgtttaat
     1201 tacctggagc acctgcctga aatcactttt tttcaggttg gaccggtgcc accatggact
     1261 ataaggacca cgacggagac tacaaggatc atgatattga ttacaaagac gatgacgata
     1321 agatggcccc aaagaagaag cggaaggtcg gtatccacgg agtcccagca gccgacaaga
     1381 agtacagcat cggcctggac atcggcacca actctgtggg ctgggccgtg atcaccgacg
     1441 agtacaaggt gcccagcaag aaattcaagg tgctgggcaa caccgaccgg cacagcatca
     1501 agaagaacct gatcggagcc ctgctgttcg acagcggcga aacagccgag gccacccggc
     1561 tgaagagaac cgccagaaga agatacacca gacggaagaa ccggatctgc tatctgcaag
     1621 agatcttcag caacgagatg gccaaggtgg acgacagctt cttccacaga ctggaagagt
     1681 ccttcctggt ggaagaggat aagaagcacg agcggcaccc catcttcggc aacatcgtgg
     1741 acgaggtggc ctaccacgag aagtacccca ccatctacca cctgagaaag aaactggtgg
     1801 acagcaccga caaggccgac ctgcggctga tctatctggc cctggcccac atgatcaagt
     1861 tccggggcca cttcctgatc gagggcgacc tgaaccccga caacagcgac gtggacaagc
     1921 tgttcatcca gctggtgcag acctacaacc agctgttcga ggaaaacccc atcaacgcca
     1981 gcggcgtgga cgccaaggcc atcctgtctg ccagactgag caagagcaga cggctggaaa
     2041 atctgatcgc ccagctgccc ggcgagaaga agaatggcct gttcggaaac ctgattgccc
     2101 tgagcctggg cctgaccccc aacttcaaga gcaacttcga cctggccgag gatgccaaac
     2161 tgcagctgag caaggacacc tacgacgacg acctggacaa cctgctggcc cagatcggcg
     2221 accagtacgc cgacctgttt ctggccgcca agaacctgtc cgacgccatc ctgctgagcg
     2281 acatcctgag agtgaacacc gagatcacca aggcccccct gagcgcctct atgatcaaga
     2341 gatacgacga gcaccaccag gacctgaccc tgctgaaagc tctcgtgcgg cagcagctgc
     2401 ctgagaagta caaagagatt ttcttcgacc agagcaagaa cggctacgcc ggctacattg
     2461 acggcggagc cagccaggaa gagttctaca agttcatcaa gcccatcctg gaaaagatgg
     2521 acggcaccga ggaactgctc gtgaagctga acagagagga cctgctgcgg aagcagcgga
     2581 ccttcgacaa cggcagcatc ccccaccaga tccacctggg agagctgcac gccattctgc
     2641 ggcggcagga agatttttac ccattcctga aggacaaccg ggaaaagatc gagaagatcc
     2701 tgaccttccg catcccctac tacgtgggcc ctctggccag gggaaacagc agattcgcct
     2761 ggatgaccag aaagagcgag gaaaccatca ccccctggaa cttcgaggaa gtggtggaca
     2821 agggcgcttc cgcccagagc ttcatcgagc ggatgaccaa cttcgataag aacctgccca
     2881 acgagaaggt gctgcccaag cacagcctgc tgtacgagta cttcaccgtg tataacgagc
     2941 tgaccaaagt gaaatacgtg accgagggaa tgagaaagcc cgccttcctg agcggcgagc
     3001 agaaaaaggc catcgtggac ctgctgttca agaccaaccg gaaagtgacc gtgaagcagc
     3061 tgaaagagga ctacttcaag aaaatcgagt gcttcgactc cgtggaaatc tccggcgtgg
     3121 aagatcggtt caacgcctcc ctgggcacat accacgatct gctgaaaatt atcaaggaca
     3181 aggacttcct ggacaatgag gaaaacgagg acattctgga agatatcgtg ctgaccctga
     3241 cactgtttga ggacagagag atgatcgagg aacggctgaa aacctatgcc cacctgttcg
     3301 acgacaaagt gatgaagcag ctgaagcggc ggagatacac cggctggggc aggctgagcc
     3361 ggaagctgat caacggcatc cgggacaagc agtccggcaa gacaatcctg gatttcctga
     3421 agtccgacgg cttcgccaac agaaacttca tgcagctgat ccacgacgac agcctgacct
     3481 ttaaagagga catccagaaa gcccaggtgt ccggccaggg cgatagcctg cacgagcaca
     3541 ttgccaatct ggccggcagc cccgccatta agaagggcat cctgcagaca gtgaaggtgg
     3601 tggacgagct cgtgaaagtg atgggccggc acaagcccga gaacatcgtg atcgaaatgg
     3661 ccagagagaa ccagaccacc cagaagggac agaagaacag ccgcgagaga atgaagcgga
     3721 tcgaagaggg catcaaagag ctgggcagcc agatcctgaa agaacacccc gtggaaaaca
     3781 cccagctgca gaacgagaag ctgtacctgt actacctgca gaatgggcgg gatatgtacg
     3841 tggaccagga actggacatc aaccggctgt ccgactacga tgtggaccat atcgtgcctc
     3901 agagctttct gaaggacgac tccatcgaca acaaggtgct gaccagaagc gacaagaacc
     3961 ggggcaagag cgacaacgtg ccctccgaag aggtcgtgaa gaagatgaag aactactggc
     4021 ggcagctgct gaacgccaag ctgattaccc agagaaagtt cgacaatctg accaaggccg
     4081 agagaggcgg cctgagcgaa ctggataagg ccggcttcat caagagacag ctggtggaaa
     4141 cccggcagat cacaaagcac gtggcacaga tcctggactc ccggatgaac actaagtacg
     4201 acgagaatga caagctgatc cgggaagtga aagtgatcac cctgaagtcc aagctggtgt
     4261 ccgatttccg gaaggatttc cagttttaca aagtgcgcga gatcaacaac taccaccacg
     4321 cccacgacgc ctacctgaac gccgtcgtgg gaaccgccct gatcaaaaag taccctaagc
     4381 tggaaagcga gttcgtgtac ggcgactaca aggtgtacga cgtgcggaag atgatcgcca
     4441 agagcgagca ggaaatcggc aaggctaccg ccaagtactt cttctacagc aacatcatga
     4501 actttttcaa gaccgagatt accctggcca acggcgagat ccggaagcgg cctctgatcg
     4561 agacaaacgg cgaaaccggg gagatcgtgt gggataaggg ccgggatttt gccaccgtgc
     4621 ggaaagtgct gagcatgccc caagtgaata tcgtgaaaaa gaccgaggtg cagacaggcg
     4681 gcttcagcaa agagtctatc ctgcccaaga ggaacagcga taagctgatc gccagaaaga
     4741 aggactggga ccctaagaag tacggcggct tcgacagccc caccgtggcc tattctgtgc
     4801 tggtggtggc caaagtggaa aagggcaagt ccaagaaact gaagagtgtg aaagagctgc
     4861 tggggatcac catcatggaa agaagcagct tcgagaagaa tcccatcgac tttctggaag
     4921 ccaagggcta caaagaagtg aaaaaggacc tgatcatcaa gctgcctaag tactccctgt
     4981 tcgagctgga aaacggccgg aagagaatgc tggcctctgc cggcgaactg cagaagggaa
     5041 acgaactggc cctgccctcc aaatatgtga acttcctgta cctggccagc cactatgaga
     5101 agctgaaggg ctcccccgag gataatgagc agaaacagct gtttgtggaa cagcacaagc
     5161 actacctgga cgagatcatc gagcagatca gcgagttctc caagagagtg atcctggccg
     5221 acgctaatct ggacaaagtg ctgtccgcct acaacaagca ccgggataag cccatcagag
     5281 agcaggccga gaatatcatc cacctgttta ccctgaccaa tctgggagcc cctgccgcct
     5341 tcaagtactt tgacaccacc atcgaccgga agaggtacac cagcaccaaa gaggtgctgg
     5401 acgccaccct gatccaccag agcatcaccg gcctgtacga gacacggatc gacctgtctc
     5461 agctgggagg cgacaaaagg ccggcggcca cgaaaaaggc cggccaggca aaaaagaaaa
     5521 agtaagaatt cctagagctc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     5581 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     5641 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     5701 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gagaatagca ggcatgctgg
     5761 ggagcggccg caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc
     5821 gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc
     5881 agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt ttctccttac
     5941 gcatctgtgc ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc
     6001 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     6061 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     6121 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     6181 ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag
     6241 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     6301 actggaacaa cactcaaccc tatctcgggc tattcttttg atttaaattc taccgggtag
     6361 gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt
     6421 ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac
     6481 cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt
     6541 tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca
     6601 ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca
     6661 gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt
     6721 ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag
     6781 gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat
     6841 ctccgggcct ttcgacctgc agcccaagct tccaccatgg tgagcaaggg cgaggagctg
     6901 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     6961 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagctgatc
     7021 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gggctacggc
     7081 ctgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     7141 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     7201 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     7261 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     7321 cacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa cttcaagatc
     7381 cgccacaaca tcgaggacgg cggcgtgcag ctcgccgacc actaccagca gaacaccccc
     7441 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagctacca gtccgccctg
     7501 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     7561 gggatcactc tcggcatgga cgagctgtac aagtaataag ggattttgcc gatttcggcc
     7621 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     7681 acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     7741 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     7801 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     7861 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     7921 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     7981 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     8041 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     8101 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     8161 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     8221 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     8281 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     8341 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     8401 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     8461 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     8521 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     8581 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     8641 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     8701 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     8761 tttattgctg ataaatctgg agccggtgag cgtggaagcc gcggtatcat tgcagcactg
     8821 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     8881 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     8941 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     9001 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     9061 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     9121 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     9181 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     9241 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     9301 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     9361 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     9421 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     9481 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     9541 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     9601 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     9661 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     9721 ttacggttcc tggccttttg ctggcctttt gctcacatgt
//
