LOCUS       Exported                7513 bp ds-DNA     circular SYN 30-JAN-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7513)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jan 30, 2023 from SnapGene Viewer 5.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7513
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    142..149
                     /locus_tag="NotI"
                     /label=NotI
     promoter        154..1881
                     /locus_tag="CAGGS promoter"
                     /label=CAGGS promoter
     misc_feature    1881..1886
                     /locus_tag="EcoRI"
                     /label=EcoRI
     misc_feature    1887..1920
                     /locus_tag="loxP"
                     /label=loxP
     misc_feature    1971..2004
                     /locus_tag="lox2272"
                     /label=lox2272
     gene            2011..2760
                     /locus_tag="TurboFP635"
                     /label=TurboFP635
     gene            complement(2767..3492)
                     /locus_tag="Kozak-EGFP(R)"
                     /label=Kozak-EGFP(R)
     misc_feature    complement(3527..3560)
                     /locus_tag="loxP(R)"
                     /label=loxP(R)
     misc_feature    complement(3611..3644)
                     /locus_tag="lox2272(R)"
                     /label=lox2272(R)
     misc_feature    3659..4251
                     /locus_tag="WPRE"
                     /label=WPRE
     polyA_signal    4258..4736
                     /locus_tag="hGH pA"
                     /label=hGH pA
     misc_feature    5819..6688
                     /locus_tag="Amp"
                     /label=Amp
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtcgaca ttgattattg actagttatt
      181 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      241 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      301 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      361 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      421 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      481 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtcg
      541 aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt
      601 tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggggggc
      661 gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg agaggtgcgg
      721 cggcagccaa tcagagcggc gcgctccgaa agtttccttt tatggcgagg cggcggcggc
      781 ggcggcccta taaaaagcga agcgcgcggc gggcgggagt cgctgcgcgc tgccttcgcc
      841 ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg accgcgttac
      901 tcccacaggt gagcgggcgg gacggccctt ctcctccggg ctgtaattag cgcttggttt
      961 aatgacggct tgtttctttt ctgtggctgc gtgaaagcct tgaggggctc cgggagggcc
     1021 ctttgtgcgg ggggagcggc tcggggggtg cgtgcgtgtg tgtgtgcgtg gggagcgccg
     1081 cgtgcggctc cgcgctgccc ggcggctgtg agcgctgcgg gcgcggcgcg gggctttgtg
     1141 cgctccgcag tgtgcgcgag gggagcgcgg ccgggggcgg tgccccgcgg tgcggggggg
     1201 gctgcgaggg gaacaaaggc tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg
     1261 tgggcgcgtc ggtcgggctg caaccccccc tgcacccccc tccccgagtt gctgagcacg
     1321 gcccggcttc gggtgcgggg ctccgtacgg ggcgtggcgc ggggctcgcc gtgccgggcg
     1381 gggggtggcg gcaggtgggg gtgccgggcg gggcggggcc gcctcgggcc ggggagggct
     1441 cgggggaggg gcgcggcggc ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca
     1501 gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct
     1561 gtgcggagcc gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc ggggcgaagc
     1621 ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc
     1681 gtccccttct ccctctccag cctcggggct gtccgcgggg ggacggctgc cttcgggggg
     1741 gacggggcag ggcggggttc ggcttctggc gtgtgaccgg cggctctaga gcctctgcta
     1801 accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgc
     1861 tgtctcatca ttttggcaaa gaattcataa cttcgtatag catacattat acgaagttat
     1921 gcagaatggt agctggattg tagctgctat tagcaatatg aaacctctta ataacttcgt
     1981 ataggatact ttatacgaag ttatggatcc cctagcgcta ccggtcgcca ccatggtggg
     2041 tgaggatagc gtgctgatca ccgagaacat gcacatgaaa ctgtacatgg agggcaccgt
     2101 gaacgaccac cacttcaagt gcacatccga gggcgaaggc aagccctacg agggcaccca
     2161 gaccatgaag atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg acatcctggc
     2221 taccagcttc atgtacggca gcaaaacctt tatcaaccac acccagggca tccccgactt
     2281 ctttaagcag tccttccctg agggcttcac atgggagagg atcaccacat acgaagacgg
     2341 gggcgtgctg accgctaccc aggacaccag cctccagaac ggctgcctca tctacaacgt
     2401 caagatcaac ggggtgaact tcccatccaa cggccctgtg atgcagaaga aaacactcgg
     2461 ctgggaggcc agcaccgaga tgctgtaccc cgctgacagc ggcctgagag gccatagcca
     2521 gatggccctg aagctcgtgg gcgggggcta cctgcactgc tccctcaaga ccacatacag
     2581 atccaagaaa cccgctaaga acctcaagat gcccggcttc tacttcgtgg acaggagact
     2641 ggaaagaatc aaggaggccg acaaagagac ctacgtcgag cagcacgaga tggctgtggc
     2701 caggtactgc gacctgccta gcaaactggg gcacagctga tgcggccgcg acgctagcgc
     2761 agatccttac ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc
     2821 cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg ctcagggcgg actgggtgct
     2881 caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt tctgctggta
     2941 gtggtcggcg agctgcacgc tgccgtcctc gatgttgtgg cggatcttga agttcacctt
     3001 gatgccgttc ttctgcttgt cggccatgat atagacgttg tggctgttgt agttgtactc
     3061 cagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg atgcccttca gctcgatgcg
     3121 gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg gtcttgtagt tgccgtcgtc
     3181 cttgaagaag atggtgcgct cctggacgta gccttcgggc atggcggact tgaagaagtc
     3241 gtgctgcttc atgtggtcgg ggtagcggct gaagcactgc acgccgtagg tcagggtggt
     3301 cacgagggtg ggccagggca cgggcagctt gccggtggtg cagatgaact tcagggtcag
     3361 cttgccgtag gtggcatcgc cctcgccctc gccggacacg ctgaacttgt ggccgtttac
     3421 gtcgccgtcc agctcgacca ggatgggcac caccccggtg aacagctcct cgcccttgct
     3481 caccatggtg gcaagcttgc ctcgagcagc gctgctcgag agatctataa cttcgtataa
     3541 tgtatgctat acgaagttat ttgccttaac ccagaaatta tcactgttat tctttagaat
     3601 ggtgcaaaga ataacttcgt ataaagtatc ctatacgaag ttatgcggcc gcatcgatta
     3661 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     3721 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     3781 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     3841 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     3901 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     3961 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     4021 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg
     4081 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     4141 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     4201 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat ctgatctacg
     4261 ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc cctggaagtt gccactccag
     4321 tgcccaccag ccttgtccta ataaaattaa gttgcatcat tttgtctgac taggtgtcct
     4381 tctataatat tatggggtgg aggggggtgg tatggagcaa ggggcaagtt gggaagacaa
     4441 cctgtagggc ctgcggggtc tattgggaac caagctggag tgcagtggca caatcttggc
     4501 tcactgcaat ctccgcctcc tgggttcaag cgattctcct gcctcagcct cccgagttgt
     4561 tgggattcca ggcatgcatg accaggctca gctaattttt gtttttttgg tagagacggg
     4621 gtttcaccat attggccagg ctggtctcca actcctaatc tcaggtgatc tacccacctt
     4681 ggcctcccaa attgctggga ttacaggcgt gaaccactgc tcccttccct gtccttctga
     4741 ttttgtaggt aaccacgtgc ggaccgagcg gccgcaggaa cccctagtga tggagttggc
     4801 cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg
     4861 cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg
     4921 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag
     4981 caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     5041 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     5101 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     5161 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca
     5221 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     5281 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc gggctattct
     5341 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     5401 caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc
     5461 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
     5521 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     5581 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
     5641 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     5701 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     5761 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     5821 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     5881 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     5941 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     6001 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     6061 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     6121 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     6181 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     6241 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     6301 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     6361 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     6421 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     6481 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     6541 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     6601 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     6661 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     6721 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     6781 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     6841 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     6901 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     6961 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     7021 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     7081 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     7141 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     7201 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     7261 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     7321 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     7381 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     7441 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     7501 tttgctcaca tgt
//
