LOCUS       Exported                8415 bp ds-DNA     circular SYN 30-JAN-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8415)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jan 30, 2023 from SnapGene Viewer 5.2.1
            https://www.snapgene.com
COMMENT     CCTAGCGCTACCGGTCGCCACCTAGGTGGGTGAGGATAGCGTGCTGATCACCGAGAACATGCACATG 
            AAACTGTACATGGAGGGCACCGTGAACGACCACCACTTCAAGTGCACATCCGAGGGCGAAGGCAAGC 
            CCTACGAGGGCACCCAGACCATGAAGATCAAGGTGGTCGAGGGCGGCCCTCTCCCCTTCGCCTTCGA 
            CATCCTGGCTACCAGCTTCTAGTACGGCAGCAAAACCTTTATCAACCACACCCAGGGCATCCCCGAC 
            TTCTTTAAGCAGTCCTTCCCTGAGGGCTTCACATGGGAGAGGATCACCACATACGAAGACGGGGGCG 
            TGCTGACCGCTACCCAGGACACCAGCCTCCAGAACGGCTGCCTCATCTACAACGTCAAGATCAACGG 
            GGTGAACTTCCCATCCAACGGCCCTGTGATGCAGAAGAAAACACTCGGCTGGGAGGCCAGCACCGAG 
            ATGCTGTACCCCGCTGACAGCGGCCTGAGAGGCCATAGCCAGATGGCCCTGAAGCTCGTGGGCGGGG 
            GCTACCTGCACTGCTCCCTCAAGACCACATACAGATCCAAGAAACCCGCTAAGAACCTCAAGATGCC 
            CGGCTTCTACTTCGTGGACAGGAGACTGGAAAGAATCAAGGAGGCCGACAAAGAGACCTACGTCGAG 
            CAGCACGAGATGGCTGTGGCCAGGTACTGCGACCTGCCTAGCAAACTGGGGCACAGCTGATGCGGCC 
            GCGACGCTAGCGC
FEATURES             Location/Qualifiers
     source          1..8415
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    131..138
                     /locus_tag="NotI"
                     /label=NotI
     promoter        143..1326
                     /locus_tag="EF1a promoter"
                     /label=EF1a promoter
     misc_feature    1327..1332
                     /locus_tag="EcoRI"
                     /label=EcoRI
     misc_feature    1333..1366
                     /locus_tag=" loxP"
                     /label=loxP
                     /label= loxP
     misc_feature    1417..1450
                     /locus_tag=" lox2272"
                     /label=lox2272
                     /label= lox2272
     misc_feature    1451..1468
                     /locus_tag=" MCS"
                     /label=MCS
                     /label= MCS
     gene            complement(1469..2188)
                     /locus_tag="EGFP(R)"
                     /label=EGFP(R)
     misc_feature    complement(2189..2251)
                     /locus_tag="2A(R)"
                     /label=2A(R)
     gene            complement(2252..3688)
                     /locus_tag="hM4D(R)"
                     /label=hM4D(R)
     misc_feature    complement(3689..3694)
                     /locus_tag="Kozak(R)"
                     /label=Kozak(R)
     misc_feature    3695..3728
                     /locus_tag=" MCS(1)"
                     /label=MCS
                     /label= MCS(1)
     misc_feature    complement(3729..3762)
                     /locus_tag=" loxP(R)"
                     /label=loxP(R)
                     /label= loxP(R)
     misc_feature    3763..3784
                     /locus_tag="5' connector"
                     /label=5' connector
     misc_feature    3772..3777
                     /locus_tag="AgeI"
                     /label=AgeI
     misc_feature    3785..4492
                     /locus_tag="TurboFP635"
                     /label=TurboFP635
     misc_feature    3785..3787
                     /locus_tag="ATG mutation1"
                     /label=ATG mutation1
     misc_feature    3983..3985
                     /locus_tag="ATG mutation2"
                     /label=ATG mutation2
     misc_feature    4493..4512
                     /locus_tag="3'connector"
                     /label=3'connector
     misc_feature    complement(4513..4546)
                     /locus_tag="lox2272(R)"
                     /label=lox2272(R)
     misc_feature    4547..4554
                     /locus_tag="NotI(1)"
                     /label=NotI
                     /label=NotI(1)
     misc_feature    4555..4560
                     /locus_tag="ClaI"
                     /label=ClaI
     misc_feature    4561..5153
                     /locus_tag=" WPRE"
                     /label=WPRE
                     /label= WPRE
     polyA_signal    5160..5638
                     /locus_tag="hGH polyA"
                     /label=hGH polyA
     gene            6721..7590
                     /locus_tag="Amp"
                     /label=Amp
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gccgtgaggc tccggtgccc gtcagtgggc agagcgcaca
      181 tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga
      241 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag
      301 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg
      361 tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg cctctttacg
      421 ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt gattcttgat
      481 cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct
      541 tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg
      601 tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga
      661 tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg
      721 cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc
      781 acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct
      841 caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg
      901 gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc
      961 cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca
     1021 cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag
     1081 taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta
     1141 ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa
     1201 gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga
     1261 tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg
     1321 tcgtgagaat tcataacttc gtatagcata cattatacga agttatgcag aatggtagct
     1381 ggattgtagc tgctattagc aatatgaaac ctcttaataa cttcgtatag gatactttat
     1441 acgaagttat ggatcctcta gagtcgactt acttgtacag ctcgtccatg ccgagagtga
     1501 tcccggcggc ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg ttggggtctt
     1561 tgctcagggc ggactgggtg ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc
     1621 cgatgggggt gttctgctgg tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt
     1681 ggcggatctt gaagttcacc ttgatgccgt tcttctgctt gtcggccatg atatagacgt
     1741 tgtggctgtt gtagttgtac tccagcttgt gccccaggat gttgccgtcc tccttgaagt
     1801 cgatgccctt cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc
     1861 gggtcttgta gttgccgtcg tccttgaaga agatggtgcg ctcctggacg tagccttcgg
     1921 gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg ctgaagcact
     1981 gcacgccgta ggtcagggtg gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg
     2041 tgcagatgaa cttcagggtc agcttgccgt aggtggcatc gccctcgccc tcgccggaca
     2101 cgctgaactt gtggccgttt acgtcgccgt ccagctcgac caggatgggc accaccccgg
     2161 tgaacagctc ctcgcccttg ctcaccattg ggccaggatt ctcctcgacg tcaccgcatg
     2221 ttagcagact tcctctgccc tctccactgc ccctggcagt gccgatgttc cgatactggc
     2281 acagcagcag gtgccggaag gtctttttaa aggtggcgtt gcacagagca tagcaggcag
     2341 ggttgatggt gctgttgacg tagcagagcc agtagccaat ggaccacacc gtgtcaggga
     2401 tgcagctctg gcagaaggtg ttcaccagga ccatgacgtt gtagggcgtc caggtgagga
     2461 tgaaggccag cagaatggca aagatcgttc gtgtcacttt gcgctcccgg gccgccatct
     2521 gccgcttctt gcgcacctgg ttgcgagcga tgctggcgaa cttgcgggcc acgttggccg
     2581 cagggcgcat gccagccggc gtggcaggca caatctcaat ggctgtcaca cactcattgc
     2641 ctgtctgctt cgtcacaatc tggatcttgg accatctgga ggctgggttg agggcccgcg
     2701 gctgcagggg aggggcgggc atggcgggcg tggtggcctc tgtggtggac agctctgtgg
     2761 ctgggcgttc cttggtgttc tgggtggcac tgcctgagct ggactcattg gaagtgtcct
     2821 tatcagccac ggggcgcggt ggcggtggca gcgctggcgg gggggcctcc tccagcttgc
     2881 cattgcgcag ctcctcccgg gcggcctccc cgggcggggg cttcttgacg ctctgcttca
     2941 ttagtgggct cttgaggaag gccagcgtct tggctttctt ctccttcggg ccctcgggcc
     3001 ggtgcttgtg gactcggctg cgactggcca gggagatgtg gatgtacagc accgtcatga
     3061 tgaccacagg caggtagaag ccagcaatgg ctgtgccaaa ggtcactgct gggttggaca
     3121 ggaactggat gaagcactgg ttgtcgggca ccgtccgctt acccaccaca aactgccaga
     3181 acaagatggc aggcgcccag agcacgaagg acagtaccca ggcagcagca atcatgaggc
     3241 ctgccatctt ggtggtgcgc cgggcagggt aggtgagagg cttggtgacg cagaagtagc
     3301 ggtcaaagct gatgatgaga aggttcatga cggaggcgtt gctcaccacg cagtccaggg
     3361 ccagccacag gtcgcagacc acggcgccca ggggccagta gcccttgatg atgtacacgg
     3421 tgtagaggtt catggagaag gcgcctatga tgagatcagc acacgccagg ctgaagagga
     3481 agtagttgtt gactgtctgc agctgcctgt tgaccttgat ggacagcatc accaggatgt
     3541 tgcccacgac agtcaccagg ctcagggagc ctgtcactgt ggcaatgaag accatttcca
     3601 ccgtctcata gcgattgtgg gatgatgacg tgaccaggcg cacggactga ttgcccgagc
     3661 tgccattgac aggtgtgaag ttggccatgg tggcaagctt gcctcgagca gcgctgctcg
     3721 agagatctat aacttcgtat aatgtatgct atacgaagtt atcctagcgc taccggtcgc
     3781 cacctaggtg ggtgaggata gcgtgctgat caccgagaac atgcacatga aactgtacat
     3841 ggagggcacc gtgaacgacc accacttcaa gtgcacatcc gagggcgaag gcaagcccta
     3901 cgagggcacc cagaccatga agatcaaggt ggtcgagggc ggccctctcc ccttcgcctt
     3961 cgacatcctg gctaccagct tctagtacgg cagcaaaacc tttatcaacc acacccaggg
     4021 catccccgac ttctttaagc agtccttccc tgagggcttc acatgggaga ggatcaccac
     4081 atacgaagac gggggcgtgc tgaccgctac ccaggacacc agcctccaga acggctgcct
     4141 catctacaac gtcaagatca acggggtgaa cttcccatcc aacggccctg tgatgcagaa
     4201 gaaaacactc ggctgggagg ccagcaccga gatgctgtac cccgctgaca gcggcctgag
     4261 aggccatagc cagatggccc tgaagctcgt gggcgggggc tacctgcact gctccctcaa
     4321 gaccacatac agatccaaga aacccgctaa gaacctcaag atgcccggct tctacttcgt
     4381 ggacaggaga ctggaaagaa tcaaggaggc cgacaaagag acctacgtcg agcagcacga
     4441 gatggctgtg gccaggtact gcgacctgcc tagcaaactg gggcacagct gatgcggccg
     4501 cgacgctagc gcataacttc gtataaagta tcctatacga agttatgcgg ccgcatcgat
     4561 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     4621 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     4681 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     4741 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     4801 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     4861 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     4921 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct
     4981 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     5041 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     5101 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc atctgatcta
     5161 cgggtggcat ccctgtgacc cctccccagt gcctctcctg gccctggaag ttgccactcc
     5221 agtgcccacc agccttgtcc taataaaatt aagttgcatc attttgtctg actaggtgtc
     5281 cttctataat attatggggt ggaggggggt ggtatggagc aaggggcaag ttgggaagac
     5341 aacctgtagg gcctgcgggg tctattggga accaagctgg agtgcagtgg cacaatcttg
     5401 gctcactgca atctccgcct cctgggttca agcgattctc ctgcctcagc ctcccgagtt
     5461 gttgggattc caggcatgca tgaccaggct cagctaattt ttgttttttt ggtagagacg
     5521 gggtttcacc atattggcca ggctggtctc caactcctaa tctcaggtga tctacccacc
     5581 ttggcctccc aaattgctgg gattacaggc gtgaaccact gctcccttcc ctgtccttct
     5641 gattttgtag gtaaccacgt gcggaccgag cggccgcagg aacccctagt gatggagttg
     5701 gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga
     5761 cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg
     5821 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa
     5881 agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     5941 gcagcgtgac cgctacactt gccagcgcct tagcgcccgc tcctttcgct ttcttccctt
     6001 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     6061 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt
     6121 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     6181 tctttaatag tggactcttg ttccaaactg gaacaacact caactctatc tcgggctatt
     6241 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     6301 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc
     6361 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
     6421 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     6481 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
     6541 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     6601 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     6661 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     6721 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     6781 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     6841 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     6901 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     6961 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     7021 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     7081 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     7141 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     7201 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     7261 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     7321 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     7381 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     7441 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     7501 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     7561 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     7621 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     7681 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     7741 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     7801 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     7861 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     7921 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     7981 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     8041 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     8101 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     8161 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     8221 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     8281 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     8341 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     8401 cttttgctca catgt
//
