LOCUS       Exported                5876 bp ds-DNA     circular SYN 30-JAN-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jan 30, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5876
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..589
                     /locus_tag="CMV promotor"
                     /label=CMV promotor
     promoter        10..562
                     /locus_tag="CMV immearly promotor"
                     /label=CMV immearly promotor
     enhancer        65..352
                     /locus_tag="CAG enhancer"
                     /label=CAG enhancer
     protein_bind    633..642
                     /locus_tag="Kozak sequence(1)"
                     /label=Kozak sequence
                     /label=Kozak sequence(1)
     sig_peptide     639..713
                     /locus_tag="GPIsp"
                     /label=GPIsp
     misc_feature    750..759
                     /locus_tag="Kozak sequence"
                     /label=Kozak sequence
     misc_feature    756..1472
                     /locus_tag="Gamillus(1)"
                     /label=Gamillus
                     /label=Gamillus(1)
     misc_feature    1503..2219
                     /locus_tag="Gamillus"
                     /label=Gamillus
     gene            2220..2327
                     /locus_tag="GPI"
                     /label=GPI
     primer_bind     2740..2760
                     /locus_tag="SV40"
                     /label=SV40
     rep_origin      complement(2924..3230)
                     /direction=LEFT
                     /locus_tag="F1 ori"
                     /label=F1 ori
     promoter        complement(3309..3337)
                     /locus_tag="AmpR promotor"
                     /label=AmpR promotor
     enhancer        3409..3624
                     /locus_tag="SV40 enhancer"
                     /label=SV40 enhancer
     promoter        3541..3737
                     /locus_tag="SV40 early promoter"
                     /label=SV40 early promoter
     rep_origin      3588..3665
                     /locus_tag="SV40 origin"
                     /label=SV40 origin
     CDS             3775..4566
                     /locus_tag="Kan/neoR"
                     /label=Kan/neoR
     terminator      4741..5010
                     /locus_tag="TK PA terminator"
                     /label=TK PA terminator
     polyA_signal    4798..4845
                     /locus_tag="HSV TK poly(A)signal"
                     /label=HSV TK poly(A)signal
     rep_origin      5098..5780
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttagc tagccaccat ggagctcttt tggagtatag
      661 tctttactgt cctcctgagt ttctcctgcc gggggtcaga ctgggaatct ctgcagtcga
      721 cggtaccgcg ggcccgggat ccaccggtcg ccaccatggt gagcaagggc gaggaggcat
      781 ctggcagagc cctgttccag taccccatga ccagcaagat cgagctgaac ggcgagatca
      841 acggcaagaa attcaaggtg gccggcgagg gcttcacccc cagcagcggc agattcaaca
      901 tgcacgccta ctgcaccacc ggcgacctgc ctatgagctg ggtcgtgatt gccagccccc
      961 tccagtacgg cttccacatg ttcgcccact accccgagga catcacacac tttttccagg
     1021 aatgcttccc cggcagctac accctggacc ggaccctgag aatggaaggc gacggcaccc
     1081 tgaccaccca ccacgagtac agcctggagg acggctgcgt gacctccaag accaccctga
     1141 atgccagcgg cttcgaccct aagggcgcca ccatgaccaa gagcttcgtg aaacaactgc
     1201 ctaacgaggt gaagatcacc ccccacggcc ccaacggcat cagactgacc agcaccgtgc
     1261 tgtacctgaa ggaggatggc accatccaga tcggcaccca ggactgcatc gtgacccctg
     1321 tgggcggaag gaaagtgacc cagcccaagg cccacttcct gcacacccag atcatccaga
     1381 agaaggaccc caacgacacc cgggaccaca tcgtgcagac agaactggcc gtggccggca
     1441 atctgtggca cggcatggac gagctgtaca agccgcctgc cgctgcacct ccggaacgcg
     1501 tcatggtgag caagggcgag gaggcatctg gcagagccct gttccagtac cccatgacca
     1561 gcaagatcga gctgaacggc gagatcaacg gcaagaaatt caaggtggcc ggcgagggct
     1621 tcacccccag cagcggcaga ttcaacatgc acgcctactg caccaccggc gacctgccta
     1681 tgagctgggt cgtgattgcc agccccctcc agtacggctt ccacatgttc gcccactacc
     1741 ccgaggacat cacacacttt ttccaggaat gcttccccgg cagctacacc ctggaccgga
     1801 ccctgagaat ggaaggcgac ggcaccctga ccacccacca cgagtacagc ctggaggacg
     1861 gctgcgtgac ctccaagacc accctgaatg ccagcggctt cgaccctaag ggcgccacca
     1921 tgaccaagag cttcgtgaaa caactgccta acgaggtgaa gatcaccccc cacggcccca
     1981 acggcatcag actgaccagc accgtgctgt acctgaagga ggatggcacc atccagatcg
     2041 gcacccagga ctgcatcgtg acccctgtgg gcggaaggaa agtgacccag cccaaggccc
     2101 acttcctgca cacccagatc atccagaaga aggaccccaa cgacacccgg gaccacatcg
     2161 tgcagacaga actggccgtg gccggcaatc tgtggcacgg catggacgag ctgtacaagt
     2221 atgatccgcg cccaagcagc ggtcattcta gacacagata tgcacttata cccataccat
     2281 tagcagtaat tacaacatgt attgtgctgt atatgaatgt tctttaattg agaagacaat
     2341 ttcttcattt ttaggtattc tgaaatgtga cagaaaacca gacagaacca actccaattg
     2401 attggtaaca gaagatgaag acaacagcat aactaaatta ttttaaaaac taaaaagcca
     2461 tctgatttct catttgagct ttagagcaca atggatctcg agggatcttc catacctacc
     2521 aagttttgcg ccttgcaagg tcgcggccgc gactctagat cataatcagc cataccacat
     2581 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     2641 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     2701 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     2761 tgtccaaact catcaatgta tcttaaggcg taaattgtaa gcgttaatat tttgttaaaa
     2821 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
     2881 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
     2941 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
     3001 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
     3061 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
     3121 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
     3181 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
     3241 ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     3301 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     3361 tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag
     3421 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     3481 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     3541 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     3601 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     3661 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     3721 gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg catgattgaa
     3781 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     3841 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     3901 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag
     3961 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     4021 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     4081 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     4141 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     4201 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
     4261 gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat
     4321 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     4381 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     4441 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     4501 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     4561 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
     4621 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     4681 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccta
     4741 gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg
     4801 caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc
     4861 ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc
     4921 gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag
     4981 ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata ctttagattg
     5041 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     5101 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     5161 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     5221 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     5281 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     5341 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     5401 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     5461 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     5521 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     5581 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     5641 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     5701 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     5761 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     5821 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc atgcat
//
