LOCUS       Exported                5129 bp ds-DNA     circular SYN 30-JAN-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5129)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jan 30, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5129
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..589
                     /locus_tag="CMV promotor"
                     /label=CMV promotor
     promoter        10..562
                     /locus_tag="CMV immearly promotor"
                     /label=CMV immearly promotor
     enhancer        65..352
                     /locus_tag="CAG enhancer"
                     /label=CAG enhancer
     protein_bind    633..642
                     /locus_tag="Kozak sequence(1)"
                     /label=Kozak sequence
                     /label=Kozak sequence(1)
     sig_peptide     639..713
                     /locus_tag="GPIsp"
                     /label=GPIsp
     misc_feature    750..759
                     /locus_tag="Kozak sequence"
                     /label=Kozak sequence
     misc_feature    756..1472
                     /locus_tag="mGreenLantern"
                     /label=mGreenLantern
     gene            1473..1580
                     /locus_tag="GPI"
                     /label=GPI
     primer_bind     1993..2013
                     /locus_tag="SV40"
                     /label=SV40
     rep_origin      complement(2177..2483)
                     /direction=LEFT
                     /locus_tag="F1 ori"
                     /label=F1 ori
     promoter        complement(2562..2590)
                     /locus_tag="AmpR promotor"
                     /label=AmpR promotor
     enhancer        2662..2877
                     /locus_tag="SV40 enhancer"
                     /label=SV40 enhancer
     promoter        2794..2990
                     /locus_tag="SV40 early promoter"
                     /label=SV40 early promoter
     rep_origin      2841..2918
                     /locus_tag="SV40 origin"
                     /label=SV40 origin
     CDS             3028..3819
                     /locus_tag="Kan/neoR"
                     /label=Kan/neoR
     terminator      3994..4263
                     /locus_tag="TK PA terminator"
                     /label=TK PA terminator
     polyA_signal    4051..4098
                     /locus_tag="HSV TK poly(A)signal"
                     /label=HSV TK poly(A)signal
     rep_origin      4351..5033
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttagc tagccaccat ggagctcttt tggagtatag
      661 tctttactgt cctcctgagt ttctcctgcc gggggtcaga ctgggaatct ctgcagtcga
      721 cggtaccgcg ggcccgggat ccaccggtcg ccaccatggt gagcaagggc gaggagctgt
      781 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
      841 gcgtccgcgg cgagggcgag ggcgatgcca ccaacggcaa gctgaccctg aagttcatct
      901 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccacctta ggctacggcg
      961 tggcctgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     1021 tgcccgaagg ctacgtccag gagcgcacca tctctttcaa ggacgacggt acctacaaga
     1081 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgtg ctgaagggca
     1141 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac ttcaacagcc
     1201 acaaggtcta tatcacggcc gacaagcaga agaacggcat caaggctaac ttcaagaccc
     1261 gccacaacgt tgaggacggc ggcgtgcagc tcgccgacca ctaccagcag aacaccccca
     1321 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagccatcag tccaaactga
     1381 gcaaagaccc caacgagaag cgcgatcaca tggtcctgaa ggagagggtg accgccgccg
     1441 ggattacaca tgacatggac gagctgtaca agtatgatcc gcgcccaagc agcggtcatt
     1501 ctagacacag atatgcactt atacccatac cattagcagt aattacaaca tgtattgtgc
     1561 tgtatatgaa tgttctttaa ttgagaagac aatttcttca tttttaggta ttctgaaatg
     1621 tgacagaaaa ccagacagaa ccaactccaa ttgattggta acagaagatg aagacaacag
     1681 cataactaaa ttattttaaa aactaaaaag ccatctgatt tctcatttga gctttagagc
     1741 acaatggatc tcgagggatc ttccatacct accaagtttt gcgccttgca aggtcgcggc
     1801 cgcgactcta gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
     1861 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
     1921 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     1981 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaag
     2041 gcgtaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
     2101 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     2161 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     2221 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     2281 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     2341 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     2401 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     2461 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga
     2521 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     2581 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tcctgaggcg
     2641 gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     2701 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     2761 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     2821 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     2881 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     2941 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa
     3001 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
     3061 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     3121 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     3181 ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg
     3241 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     3301 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     3361 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     3421 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     3481 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     3541 aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     3601 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     3661 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     3721 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     3781 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
     3841 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
     3901 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     3961 gggatctcat gctggagttc ttcgcccacc ctagggggag gctaactgaa acacggaagg
     4021 agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg
     4081 gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata
     4141 ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc
     4201 ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca
     4261 tagcctcagg ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     4321 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     4381 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     4441 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     4501 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     4561 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     4621 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     4681 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     4741 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     4801 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     4861 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     4921 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     4981 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     5041 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     5101 ctgtggataa ccgtattacc gccatgcat
//
