LOCUS       Exported                5862 bp ds-DNA     circular UNA 30-JAN-2023
DEFINITION  pMH464_pMH045_SMAC_9His.
ACCESSION   urn.local.mhunkeler.hs-fuq3fbk
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jan 30, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5862
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     promoter        1..19
                     /label=T7 prom
                     /note="/Imported_from=Pla sMapper"
                     /note="/Transferred_From=T7 prom"
                     /note="/Transferred_Similarity=100.00%"
     misc_feature    19..46
                     /label=lacO reg
                     /note="Geneious type: regulatory sequence"
                     /note="/Imported_from=Pla sMapper"
                     /note="/Transferred_From=lacO reg"
                     /note="/Transferred_Similarity=100.00%"
     misc_feature    62..700
                     /label=sequence optimized for e coli (concatenated
                     seq
                     /label=sequence optimized for e coli (concatenated
                     sequence 2)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument:
                     urn:local:mhunkeler:1by-da0o6yx"
     gene            64..80
                     /label=T7_transl_en_RBS
                     /note="/Imported_from=Pla sMapper"
                     /note="/Transferred_From=T7_transl_en_RBS"
                     /note="/Transferred_Similarity=100.00%"
     CDS             92..670
                     /label=Smac/DIABLO
                     /note="/created_by=mhunkeler"
     CDS             644..670
                     /label=9xHis
                     /note="/created_by=mhunkeler"
                     /note="/modified_by=mhunkeler"
     misc_feature    join(671..5862,1..91)
                     /label=pFI045 Nterm-his-tev C-term Avi
                     pNIC-Bio2-JF912
                     /label=pFI045 Nterm-his-tev C-term Avi
                     pNIC-Bio2-JF912191.1 PCR Product (concatenated sequence 1)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument:
                     urn:local:mhunkeler:1d2-da0owu3"
     CDS             671..673
                     /label=*
                     /note="/created_by=mhunkeler"
                     /note="/modified_by=mhunkeler"
     terminator      812..855
                     /label=T7 term
                     /note="/Imported_from=Pla sMapper"
                     /note="/Transferred_From=T7 term"
                     /note="/Transferred_Similarity=97.73%"
     misc_feature    908..1214
                     /label=f1 origin
                     /note="Geneious type: origin of replication"
                     /note="/Imported_from=Pla sMapper"
                     /note="/Transferred_From=f1 origin"
                     /note="/Transferred_Similarity=99.67%"
     misc_feature    complement(1439..2254)
                     /label=kan2 marker
                     /label=nonstandard type:  marker
                     /note="/Imported_from=Pla sMapper"
                     /note="/Transferred_From=kan2 marker"
                     /note="/Transferred_Similarity=99.75%"
     misc_feature    2360..2979
                     /label=pBR322 origin
                     /note="Geneious type: origin of replication"
                     /note="/Imported_from=Pla sMapper"
                     /note="/Transferred_From=pBR322 origin"
                     /note="/Transferred_Similarity=99.68%"
     gene            complement(3394..3585)
                     /label=ROP
                     /note="/Imported_from=Pla sMapper"
                     /note="/Transferred_From=ROP"
                     /note="/Transferred_Similarity=100.00%"
     misc_feature    complement(4394..5485)
                     /label=lacI reg
                     /note="Geneious type: regulatory sequence"
                     /note="/Imported_from=Pla sMapper"
                     /note="/Transferred_From=lacI reg"
                     /note="/Transferred_Similarity=100.00%"
ORIGIN
        1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt
       61 tgtttaactt taagaaggag atatacatat ggccgttcct atcgcgcaga aatctgaacc
      121 tcattccttg tcgtcggaag ctttgatgcg tcgtgctgta tctttagtca cggattccac
      181 ctcaactttt ttgagccaaa caacatatgc gcttattgaa gcgattacag aatacactaa
      241 agcggtgtat actctgacta gtttgtatcg ccagtatact tcccttcttg gaaaaatgaa
      301 tagtgaagaa gaggatgaag tatggcaagt tattatcgga gcccgcgcgg aaatgactag
      361 taaacatcag gagtacttga agttagaaac cacctggatg accgctgtag ggttgagtga
      421 gatggctgct gaagcggcct atcagactgg agccgatcag gcatcaatta cagcacgtaa
      481 ccacatccaa ttagtgaagc tgcaagtcga agaagtacac caactttcgc gcaaagcaga
      541 gaccaaattg gccgaggctc aaattgagga gctgcgccaa aaaacccagg aagaggggga
      601 agaacgtgca gaatcggagc aggaggctta tcttcgtgag gatcaccatc atcatcatca
      661 tcaccatcat tgataaggat ccgaattcga gctccgtcga caagcttgcg gccgcactcg
      721 agcaccacca ccaccaccac tgagatccgg ctgctaacaa agcccgaaag gaagctgagt
      781 tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct
      841 tgaggggttt tttgctgaaa ggaggaacta tatccggatt ggcgaatggg acgcgccctg
      901 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
      961 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     1021 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     1081 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     1141 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     1201 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     1261 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     1321 taacaaaata ttaacgttta caatttcagg tggcactttt cggggaaatg tgcgcggaac
     1381 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga attaattctt
     1441 agaaaaactc atcgagcatc aaatgaaact gcaatttatt catatcagga ttatcaatac
     1501 catatttttg aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg cagttccata
     1561 ggatggcaag atcctggtat cggtctgcga ttccgactcg tccaacatca atacaaccta
     1621 ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa atcaccatga gtgacgactg
     1681 aatccggtga gaatggcaaa agtttatgca tttctttcca gacttgttca acaggccagc
     1741 cattacgctc gtcatcaaaa tcactcgcat caaccaaacc gttattcatt cgtgattgcg
     1801 cctgagcgag acgaaatacg cgatcgctgt taaaaggaca attacaaaca ggaatcgaat
     1861 gcaaccggcg caggaacact gccagcgcat caacaatatt ttcacctgaa tcaggatatt
     1921 cttctaatac ctggaatgct gttttcccgg ggatcgcagt ggtgagtaac catgcatcat
     1981 caggagtacg gataaaatgc ttgatggtcg gaagaggcat aaattccgtc agccagttta
     2041 gtctgaccat ctcatctgta acatcattgg caacgctacc tttgccatgt ttcagaaaca
     2101 actctggcgc atcgggcttc ccatacaatc gatagattgt cgcacctgat tgcccgacat
     2161 tatcgcgagc ccatttatac ccatataaat cagcatccat gttggaattt aatcgcggcc
     2221 tagagcaaga cgtttcccgt tgaatatggc tcataacacc ccttgtatta ctgtttatgt
     2281 aagcagacag ttttattgtt catgaccaaa atcccttaac gtgagttttc gttccactga
     2341 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     2401 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     2461 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     2521 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     2581 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     2641 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     2701 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     2761 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     2821 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     2881 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     2941 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     3001 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     3061 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     3121 gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg
     3181 tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat
     3241 agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca
     3301 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     3361 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     3421 acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc
     3481 tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct
     3541 gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta
     3601 agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat
     3661 acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc
     3721 ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa
     3781 tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat
     3841 aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac
     3901 cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc
     3961 gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct
     4021 caacgacagg agcacgatca tgcgcacccg tggggccgcc atgccggcga taatggcctg
     4081 cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa
     4141 gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc
     4201 gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac
     4261 agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt
     4321 gaaggctctc aagggcatcg gtcgagatcc cggtgcctaa tgagtgagct aacttacatt
     4381 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     4441 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgccagg gtggtttttc
     4501 ttttcaccag tgagacgggc aacagctgat tgcccttcac cgcctggccc tgagagagtt
     4561 gcagcaagcg gtccacgctg gtttgcccca gcaggcgaaa atcctgtttg atggtggtta
     4621 acggcgggat ataacatgag ctgtcttcgg tatcgtcgta tcccactacc gagatatccg
     4681 caccaacgcg cagcccggac tcggtaatgg cgcgcattgc gcccagcgcc atctgatcgt
     4741 tggcaaccag catcgcagtg ggaacgatgc cctcattcag catttgcatg gtttgttgaa
     4801 aaccggacat ggcactccag tcgccttccc gttccgctat cggctgaatt tgattgcgag
     4861 tgagatattt atgccagcca gccagacgca gacgcgccga gacagaactt aatgggcccg
     4921 ctaacagcgc gatttgctgg tgacccaatg cgaccagatg ctccacgccc agtcgcgtac
     4981 cgtcttcatg ggagaaaata atactgttga tgggtgtctg gtcagagaca tcaagaaata
     5041 acgccggaac attagtgcag gcagcttcca cagcaatggc atcctggtca tccagcggat
     5101 agttaatgat cagcccactg acgcgttgcg cgagaagatt gtgcaccgcc gctttacagg
     5161 cttcgacgcc gcttcgttct accatcgaca ccaccacgct ggcacccagt tgatcggcgc
     5221 gagatttaat cgccgcgaca atttgcgacg gcgcgtgcag ggccagactg gaggtggcaa
     5281 cgccaatcag caacgactgt ttgcccgcca gttgttgtgc cacgcggttg ggaatgtaat
     5341 tcagctccgc catcgccgct tccacttttt cccgcgtttt cgcagaaacg tggctggcct
     5401 ggttcaccac gcgggaaacg gtctgataag agacaccggc atactctgcg acatcgtata
     5461 acgttactgg tttcacattc accaccctga attgactctc ttccgggcgc tatcatgcca
     5521 taccgcgaaa ggttttgcgc cattcgatgg tgtccgggat ctcgacgctc tcccttatgc
     5581 gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca
     5641 aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc
     5701 atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg
     5761 gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg
     5821 atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
//
