LOCUS       pCCL-c-MNDU3-CD3D_WT_cD 7210 bp ds-DNA     circular     31-JAN-2023
DEFINITION  .
KEYWORDS    "marker:AmpR, AmpR, R"
FEATURES             Location/Qualifiers
     promoter        21..39
                     /label="T3 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: T3 promoter"
     enhancer        140..519
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: CMV enhancer"
     misc_feature    209..737
                     /label="CMV"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="label: CMV"
     primer          340..362
                     /label="Seq 13 F"
                     /note="sequence: gtggagtatttacggtaaactgc"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          443..467
                     /label="NG Seq 1 F"
                     /note="sequence: attatgcccagtacatgaccttatg"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     promoter        520..723
                     /label="CMV promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: CMV promoter"
     primer          672..690
                     /label="FnCas9 1"
                     /note="sequence: gacgcaaatgggcggtagg"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          691..708
                     /label="NG Seq 2 F"
                     /note="sequence: cgtgtacggtgggaggtc"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    738..833
                     /label="R"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: R"
     LTR             738..918
                     /label="5' LTR (truncated)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="label: 5' LTR (truncated)"
     misc_feature    834..917
                     /label="U5"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="label: U5"
     misc_feature    965..1090
                     /label="HIV-1 Ψ"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="label: HIV-1 Ψ"
     misc_feature    970..1107
                     /label="PSI"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="label: PSI"
     misc_feature    1339..1363
                     /label="GS4F"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="label: GS4F"
     misc_feature    1436..1522
                     /label="dENV"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="label: dENV"
     misc_feature    1572..1771
                     /label="rre"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="label: rre"
     misc_feature    2092..2114
                     /label="GSF6"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="label: GSF6"
     misc_feature    2190..2191
                     /label="SA"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="label: SA"
     misc_feature    2308..2497
                     /label="cPPT"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="label: cPPT"
     misc_feature    complement(2341..2355)
                     /label="cPPT"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="label: cPPT"
     misc_feature    complement(2356..2360)
                     /label="PPT ter"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="label: PPT ter"
     misc_feature    2511..2654
                     /label="env-region (removable)"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="label: env-region (removable)"
     misc_feature    2511..3044
                     /label="MNDU3"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="label: MNDU3"
     misc_feature    3032..3040
                     /label="TSS"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="label: TSS"
     primer          complement(3133..3150)
                     /label="Open PCCLc for CD3d cDNA R"
                     /note="sequence: GGTGGCGACCGGTGGATC"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    3136..3150
                     /label="HA"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          3151..3171
                     /label="Codon-Optimized TIDE F"
                     /note="sequence: ATGGAGCACAGCACCTTCCTG"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    3151..3666
                     /label="CD3d cDNA "
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          3153..3171
                     /label="Codon Optimized TIDE F"
                     /note="sequence: GGAGCACAGCACCTTCCTG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          3309..3328
                     /label="HDR Thera CD3d SCID Guide 1"
                     /note="sequence: AGACATTACAAGACTGGACC"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          3310..3329
                     /label="HDR Thera CD3d SCID Guide 2"
                     /note="sequence: GACATTACAAGACTGGACCT"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(3324..3341)
                     /label="Q5 SDM for WT CD3D R"
                     /note="sequence: ATGCGTTTTCCCAGGTCC"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          3324..3343
                     /label="HDR Thera CD3d SCID Guide 3"
                     /note="sequence: GGACCTGGGAAAACGCATCC"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(3331..3348)
                     /label="SDM for WT to A0 R"
                     /note="sequence: GTCCAGGATGCGTTTTCC"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(3334..3355)
                     /label="SDM_CD3D C202T to WT and A0 R"
                     /note="sequence: CTCATGGGTCCAGGATGCGTTT"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    complement(3339..3358)
                     /label="NG1T guide"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          3342..3365
                     /label="Q5 SDM_for WT CD3D_F"
                     /note="sequence: CCTGGACCCATGAGGAATATATAG"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    3352..3354
                     /label="C202T "
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          3356..3387
                     /label="SDM_CD3D C202T to WT and A0 F"
                     /note="sequence: GAATATATAGGTGTAATGGGACAGATATATAC"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    3359..3359
                     /label="A0"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(3636..3652)
                     /label="Codon Optimize TIDE R"
                     /note="sequence: CCCAGTTGCCGCCCAGG"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    3667..3684
                     /label="HA"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          3667..3686
                     /label="GM-F- PCCLc-CD3d cDNA"
                     /note="sequence: TAAAGCGGCCAACTCGACGG"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    3760..3774
                     /label="PPT"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="label: PPT"
     LTR             3777..4010
                     /label="3' LTR (ΔU3)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="label: 3' LTR (ΔU3)"
     misc_feature    3830..3925
                     /label="R"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     polyA_signal    4082..4203
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="label: SV40 poly(A) signal"
     rep_origin      4243..4378
                     /label="SV40 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="label: SV40 ori"
     promoter        complement(4411..4429)
                     /label="T7 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: T7 promoter"
     primer_bind     complement(4439..4455)
                     /label="M13 fwd"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
                     /note="label: M13 fwd"
     primer          complement(4525..4539)
                     /label="Ralph Primer 8"
                     /note="sequence: cgctattacgccagc"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     rep_origin      4600..5055
                     /label="f1 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="label: f1 ori"
     promoter        5084..5188
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: AmpR promoter"
     primer          complement(5117..5142)
                     /label="NG Seq 14 F"
                     /note="sequence: ctcatgagcggatacatatttgaatg"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             5189..5257
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="label: AmpR"
     primer          complement(5191..5213)
                     /label="Seq 12 F"
                     /note="sequence: cgacacggaaatgttgaatactc"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             5258..6049
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="label: AmpR"
     primer          complement(5530..5555)
                     /label="BE4 Sequence 12"
                     /note="sequence: cactgcataattctcttactgtcatg"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(5794..5818)
                     /label="NG Seq 13 F"
                     /note="sequence: ctccatccagtctattaattgttgc"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(5877..5897)
                     /label="Seq 11 F"
                     /note="sequence: caccggctccagatttatcag"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     rep_origin      6220..6808
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="label: ori"
     primer          complement(6236..6255)
                     /label="BE4 GFP Sequence 12"
                     /note="sequence: aagcagcagattacgcgcag"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(6445..6463)
                     /label="NG Seq 12 F"
                     /note="sequence: ggcagcagccactggtaac"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(6571..6590)
                     /label="Seq 10 F"
                     /note="sequence: gttcggtgtaggtcgttcgc"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(6860..6880)
                     /label="BE4 GFP Sequence 11"
                     /note="sequence: cgcaggaaagaacatgtgagc"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     protein_bind    7096..7117
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="label: CAP binding site"
     promoter        7132..7137
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: lac promoter"
     promoter        7138..7155
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: lac promoter"
     promoter        7156..7162
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="label: lac promoter"
     protein_bind    7170..7186
                     /label="lac operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="label: lac operator"
     primer_bind     7194..7210
                     /label="M13 rev"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
                     /note="label: M13 rev"
ORIGIN
        1 catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa
       61 gcttggccat tgcatacgtt gtatccatat cataatatgt acatttatat tggctcatgt
      121 ccaacattac cgccatgttg acattgatta ttgactagtt attaatagta atcaattacg
      181 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
      241 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
      301 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
      361 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
      421 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
      481 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
      541 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
      601 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
      661 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
      721 gctcgtttag tgaaccgggg tctctctggt tagaccagat ctgagcctgg gagctctctg
      781 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
      841 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
      901 tgtggaaaat ctctagcagt ggcgcccgaa cagggacctg aaagcgaaag ggaaaccaga
      961 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
     1021 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
     1081 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag
     1141 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga
     1201 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg
     1261 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt
     1321 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga
     1381 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct
     1441 tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat aaatataaag
     1501 tagtaaaaat tgaaccatta ggAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA
     1561 GAGAAAAAAG AGCAGTGGGA ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA
     1621 GCACTATGGG CGCAGCCTCA ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA
     1681 TAGTGCAGCA GCAGAACAAT TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC
     1741 TCACAGTCTG GGGCATCAAG CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA
     1801 AGGATCAACA GCTCCTGGGG ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG
     1861 TGCCTTGGAA TGCTAGTTGG AGTAATAAAT CTCTGGAACA GATTGGAATC ACACGACCTG
     1921 GATGGAGTGG GACAGAGAAA TTAACAATTA CACAAGCTTA ATACACTCCT TAATTGAAGA
     1981 ATCGCAAAAC CAGCAAGAAA AGAATGAACA AGAATTATTG GAATTAGATA AATGGGCAAG
     2041 TTTGTGGAAT TGGTTTAACA TAACAAATTG GCTGTGGTAT ATAAAATTAT TCATAATGAT
     2101 AGTAGGAGGC TTGGTAGGTT TAAGAATAGT TTTTGCTGTA CTTTCTATAG TGAATAGAGT
     2161 TAGGCAGGGA TATTCACCAT TATCGTTTCA GACCCACCTC CCAACCCCGA GGGGACCCGA
     2221 CAGGCCCGAA GGAATAGAAG AAGAAGGTGG AGAGAGAGAC AGAGACAGAT CCATTCGATT
     2281 AGTGAACGGA TCTCGACGGT ATCGATCTCG ACACAAATGG CAGTATTCAT CCACAATTTT
     2341 AAAAGAAAAG GGGGGATTGG GGGGTACAGT GCAGGGGAAA GAATAGTAGA CATAATAGCA
     2401 ACAGACATAC AAACTAAAGA ATTACAAAAA CAAATTACAA AAATTCAAAA TTTTCGGGTT
     2461 TATTACAGGG ACAGCAGAGA TCCAGTTTGG GTCGAGGATA TCGGATCTAG ATCGATTAGT
     2521 CCAATTTGTT AAAGACAGGA TATCAGTGGT CCAGGCTCTA GTTTTGACTC AACAATATCA
     2581 CCAGCTGAAG CCTATAGAGT ACGAGCCATA GATAAAATAA AAGATTTTAT TTAGTCTCCA
     2641 GAAAAAGGGG GGAATGAAAG ACCCCACCTG TAGGTTTGGC AAGCTAGGAT CAAGGTCAGG
     2701 AACAGAGAAA CAGGAGAATA TGGGCCAAAC AGGATATCTG TGGTAAGCAG TTCCTGCCCC
     2761 GCTCAGGGCC AAGAACAGTT GGAACAGGAG AATATGGGCC AAACAGGATA TCTGTGGTAA
     2821 GCAGTTCCTG CCCCGCTCAG GGCCAAGAAC AGATGGTCCC CAGATGCGGT CCCGCCCTCA
     2881 GCAGTTTCTA GAGAACCATC AGATGTTTCC AGGGTGCCCC AAGGACCTGA AATGACCCTG
     2941 TGCCTTATTT GAACTAACCA ATCAGTTCGC TTCTCGCTTC TGTTCGCGCG CTTCTGCTCC
     3001 CCGAGCTCAA TAAAAGAGCC CACAACCCCT CACTCGGCGC GATCGATGAA TTCGAGCTCG
     3061 GTACCCGGGG ATCCCGGGTG ATCAGTCGAG CTCAAGCTTC GAATTCTGCA GTCGACGGTA
     3121 CCGCGGGCCC GGGATCCACC GGTCGCCACC ATGGAGCACA GCACCTTCCT GTCTGGCCTG
     3181 GTACTGGCTA CCCTTCTCTC GCAAGTGAGC CCCTTCAAGA TACCTATAGA GGAACTTGAG
     3241 GACAGAGTGT TTGTGAATTG CAATACCAGC ATCACATGGG TAGAGGGAAC GGTGGGAACA
     3301 CTGCTCTCAG ACATTACAAG ACTGGACCTG GGAAAACGCA TCCTGGACCC ACGAGGAATA
     3361 TATAGGTGTA ATGGGACAGA TATATACAAG GACAAAGAAT CTACCGTGCA AGTTCATTAT
     3421 CGAATGTGCC AGAGCTGTGT GGAGCTGGAT CCAGCCACCG TGGCTGGCAT CATTGTCACT
     3481 GATGTCATTG CCACTCTGCT CCTTGCTTTG GGAGTCTTCT GCTTTGCTGG ACATGAGACT
     3541 GGAAGGCTGT CTGGGGCTGC CGACACACAA GCTCTGTTGA GGAATGACCA GGTCTATCAG
     3601 CCCCTCCGAG ATCGAGATGA TGCTCAGTAC AGCCACCTGG GCGGCAACTG GGCTCGGAAC
     3661 AAGTGATAAA GCGGCCAACT CGACGGGCCC GCGGAATTCG AGCTCGGTAC CTTTAAGACC
     3721 AATGACTTAC AAGGCAGCTG TAGATCTTAG CCACTTTTTA AAAGAAAAGG GGGGACtgga
     3781 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     3841 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     3901 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     3961 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     4021 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     4081 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     4141 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     4201 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
     4261 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     4321 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     4381 ggcttttgcg tcgagacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac
     4441 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     4501 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     4561 cttcccaaca gttgcgcagc ctgaatggcg aatggcgcga cgcgccctgt agcggcgcat
     4621 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     4681 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     4741 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     4801 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     4861 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     4921 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     4981 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     5041 taacgtttac aatttcccag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     5101 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     5161 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     5221 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     5281 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     5341 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     5401 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     5461 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     5521 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     5581 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     5641 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     5701 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     5761 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     5821 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     5881 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     5941 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     6001 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     6061 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     6121 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     6181 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     6241 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     6301 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     6361 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     6421 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     6481 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     6541 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     6601 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     6661 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     6721 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     6781 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     6841 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     6901 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     6961 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     7021 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     7081 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     7141 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     7201 cagctatgac
//