LOCUS       SMC1675_pFBD_GST        8772 bp DNA     circular SYN 18-AUG-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 8772)
  AUTHORS   eliasa55
  TITLE     Direct Submission
  JOURNAL   Exported Feb 7, 2023 from SnapGene 6.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8772
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     rep_origin      102..557
                     /label=M13 origin
     rep_origin      119..425
                     /label=F1 ori
     CDS             887..1546
                     /label=AmpR
     rep_origin      1644..2326
                     /label=ColE1 origin
     misc_feature    4528..4549
                     /label=148 Pph_seqF
     misc_feature    4612..5274
                     /label=GST
     primer_bind     5230..5249
                     /label=GST-Fw
     misc_feature    5275..5286
                     /label=GST
                     /label=GST(1)
     primer_bind     5301..5346
                     /label=Fragment.FOR (1)
     primer_bind     5301..5331
                     /label=Fragment.FOR
     misc_feature    5302..5322
                     /label=TEV cleavage ENLYFQ^G
     primer_bind     5325..5344
                     /label=Fragment.FORa
     CDS             5329..6036
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6016..6058)
                     /label=Fragment.REV (1)
     primer_bind     complement(6016..6036)
                     /label=Fragment.REVa
     primer_bind     6037..6064
                     /label=Fragment.FOR
     misc_feature    6043..8232
                     /label=TBK1-CDS
     primer_bind     6634..6653
                     /label=TBK1 Seq Fw-1
     primer_bind     7253..7272
                     /label=TBK1 Seq Fw-2
     primer_bind     7473..7492
                     /label=TBK1 Seq Fw-3
     primer_bind     complement(8208..8256)
                     /label=Fragment.REV
     misc_feature    8267..8287
                     /label=4 SV40_seqR
     polyA_signal    complement(8301..8492)
                     /label=SV40 late polyA
     primer_bind     complement(8303..8334)
                     /label=SV40 Rv - SMP0004
ORIGIN
        1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
       61 ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
      121 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      181 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
      241 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
      301 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
      361 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
      421 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
      481 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
      541 attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      601 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      661 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      721 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
      781 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
      841 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
      901 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
      961 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     1021 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     1081 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     1141 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     1201 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     1261 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     1321 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     1381 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     1441 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     1501 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     1561 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1621 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     1681 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1741 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1801 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1861 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     1921 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     1981 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     2041 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     2101 acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     2161 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     2221 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     2281 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     2341 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     2401 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     2461 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     2521 tctgtgcggt atttcacacc gcagaccagc cgcgtaacct ggcaaaatcg gttacggttg
     2581 agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
     2641 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
     2701 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
     2761 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg
     2821 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc
     2881 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac
     2941 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc
     3001 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagga gattgatgag
     3061 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat
     3121 ctcactacgc ggctgctcaa acctgggcag aacgtaagcc gcgagagcgc caacaaccgc
     3181 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta
     3241 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag
     3301 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat
     3361 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt
     3421 gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
     3481 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga
     3541 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
     3601 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc
     3661 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg
     3721 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg
     3781 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg ccctggcttc
     3841 aggagatcgg tagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag
     3901 tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct
     3961 atagttctag tggttggcct acgtacccgt agtggctatg gcagggcttg ccgccccgac
     4021 gttggctgcg agccctgggc cttcacccga acttgggggt tggggtgggg aaaaggaaga
     4081 aacgcgggcg tattggtccc aatggggtct cggtggggta tcgacagagt gccagccctg
     4141 ggaccgaacc ccgcgtttat gaacaaacga cccaacaccc gtgcgtttta ttctgtcttt
     4201 ttattgccgt catagcgcgg gttccttccg gtattgtctc cttccgtgtt tcagttagcc
     4261 tcccccatct cccggtaccg catgctatgc atcagctgct agcaccatgg ctcgagatcc
     4321 cgggtgatca agtcttcgtc gagtgattgt aaataaaatg taatttacag tatagtattt
     4381 taattaatat acaaatgatt tgataataat tcttatttaa ctataatata ttgtgttggg
     4441 ttgaattaaa ggtccgtata ctccggaata ttaatagatc atggagataa ttaaaatgat
     4501 aaccatctcg caaataaata agtattttac tgttttcgta acagttttgt aataaaaaaa
     4561 cctataaata ttccggatta ttcataccgt cccaccatcg ggcgcggatc catggcatcc
     4621 cctatactag gttattggaa aattaagggc cttgtgcaac ccactcgact tcttttggaa
     4681 tatcttgaag aaaaatatga agagcatttg tatgagcgcg atgaaggtga taaatggcga
     4741 aacaaaaagt ttgaattggg tttggagttt cccaatcttc cttattatat tgatggtgat
     4801 gttaaattaa cacagtctat ggccatcata cgttatatag ctgacaagca caacatgttg
     4861 ggtggttgtc caaaagagcg tgcagagatt tcaatgcttg aaggagcggt tttggatatt
     4921 agatacggtg tttcgagaat tgcatatagt aaagactttg aaactctcaa agttgatttt
     4981 cttagcaagc tacctgaaat gctgaaaatg ttcgaagatc gtttatgtca taaaacatat
     5041 ttaaatggtg atcatgtaac ccatcctgac ttcatgttgt atgacgctct tgatgttgtt
     5101 ttatacatgg acccaatgtg cctggatgcg ttcccaaaat tagtttgttt taaaaaacgt
     5161 attgaagcta tcccacaaat tgataagtac ttgaaatcca gcaagtatat agcatggcct
     5221 ttgcagggct ggcaagccac gtttggtggt ggcgaccatc ctccaaaatc ggatctggtt
     5281 ccgcgtggat caggatcagg agagaattta tactttcagg gagcgcgcat ggtgagcaag
     5341 ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag
     5401 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     5461 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac
     5521 atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc
     5581 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
     5641 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc
     5701 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag
     5761 accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc
     5821 gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc
     5881 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg
     5941 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc
     6001 cgccactcca ccggcggcat ggacgagctg tacaaggcgc gcatgcaatc cacatccaac
     6061 cacctctggc tcctctcgga cattctcgga caaggcgcta ccgctaacgt gttcagggga
     6121 aggcacaaga agaccggtga cctgttcgcc atcaaggtgt tcaacaacat ctcattcctg
     6181 cgccctgtgg acgtccagat gcgtgagttc gaagtgctga agaagctgaa ccacaagaac
     6241 atcgtcaagc tgttcgccat cgaggaagaa accactaccc gccacaaggt gctgatcatg
     6301 gagttctgcc cctgcggttc cctgtacact gtcctggaag agccttccaa cgcttacggt
     6361 ctgcccgaga gcgagttcct gatcgtcctg cgtgacgtgg tcggtggcat gaaccacctg
     6421 agggaaaacg gcatcgtgca cagagacatc aagccaggaa acatcatgcg cgtgatcggc
     6481 gaggacggac agagcgtcta caagctgacc gacttcggcg ctgccagaga gctggaagac
     6541 gacgaacagt tcgtctctct gtacggaact gaagagtacc tgcaccctga catgtacgag
     6601 agggccgtgc tgagaaagga ccaccagaag aagtacggtg ctaccgtgga cctgtggtct
     6661 atcggcgtca ccttctacca cgctgccact ggatcactgc ctttccgccc cttcgaaggt
     6721 cctcgccgta acaaggaggt catgtacaag atcatcactg gcaagccctc tggcgccatc
     6781 tcaggagtcc agaaggctga aaacggtccc atcgactgga gcggcgacat gccagtgagc
     6841 tgctctctgt cccgcggtct gcaagtgctg ctgaccccag tcctggccaa catcctggaa
     6901 gctgaccagg agaagtgctg gggtttcgac cagttcttcg ctgaaacctc cgacatcctg
     6961 cacagaatgg tcatccacgt cttcagcctg caacagatga ctgcccacaa gatttacatc
     7021 cactcttaca acactgctac tatcttccac gaactggtct acaagcagac caagatcatc
     7081 tccagcaacc aggaactgat ctacgaggga aggagactgg tgctggagcc cggtcgcctg
     7141 gctcagcact tcccaaagac taccgaagag aaccctatct tcgtggtgtc ccgcgagcca
     7201 ctgaacacta tcggcctgat ctacgagaag atcagcctgc ccaaggtcca cccacgttac
     7261 gacctggacg gcgacgctag catggctaag gccatcaccg gagtggtctg ctacgcttgc
     7321 cgcatcgctt caactctgct gctgtaccag gaactgatgc gcaagggaat ccgttggctg
     7381 atcgagctga tcaaggacga ctacaacgaa actgtgcaca agaagaccga ggtggtcatc
     7441 actctggact tctgcatccg taacatcgaa aagaccgtga aggtctacga gaagctgatg
     7501 aagatcaacc tggaggctgc cgaactggga gagatcagcg acatccacac taagctgctg
     7561 cgcctgtctt catcccaggg cactatcgaa acctccctgc aagacatcga ctcccgtctg
     7621 agcccaggag gttcactggc tgacgcttgg gctcaccagg aaggcaccca ccctaaggac
     7681 aggaacgtgg agaagctgca agtcctgctg aactgcatga ctgaaatcta ctaccagttc
     7741 aagaaggaca aggccgagcg ccgtctggct tacaacgaag agcagatcca caagttcgac
     7801 aagcagaagc tgtactacca cgccaccaag gctatgactc acttcaccga cgaatgcgtc
     7861 aagaagtacg aggctttcct gaacaagagc gaagagtgga tcaggaagat gctgcacctg
     7921 agaaagcagc tgctgtccct gaccaaccag tgcttcgaca tcgaagagga agtgagcaag
     7981 taccaggagt acaccaacga gctgcaagag actctgcctc agaagatgtt cactgccagc
     8041 tctggtatca agcacactat gaccccaatc tacccttcat ccaacaccct ggtggaaatg
     8101 actctgggta tgaagaagct gaaggaggaa atggagggcg tggtcaagga actggctgag
     8161 aacaaccaca tcttggaaag attcggtagc ctgacgatgg atggtggact gagaaacgtg
     8221 gactgtctgt aaaagcttgt cgagaagtac tagaggatca taatcagcca taccacattt
     8281 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     8341 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
     8401 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     8461 tccaaactca tcaatgtatc ttatcatgtc tggatctgat cactgcttga gcctaggaga
     8521 tccgaaccag ataagtgaaa tctagttcca aactattttg tcatttttaa ttttcgtatt
     8581 agcttacgac gctacaccca gttcccatct attttgtcac tcttccctaa ataatcctta
     8641 aaaactccat ttccacccct cccagttccc aactattttg tccgcccaca gcggggcatt
     8701 tttcttcctg ttatgttttt aatcaaacat cctgccaact ccatgtgaca aaccgtcatc
     8761 ttcggctact tt
//
