LOCUS       SMC1613_GST-TEV-        8439 bp DNA     circular SYN 08-JUN-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    U926TGF080
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8439)
  AUTHORS   GenScript
  TITLE     Direct Submission
  JOURNAL   Exported Feb 7, 2023 from SnapGene 6.2.1
            https://www.snapgene.com
COMMENT     http://www.genscript.com/
            ORIGDB|GenBank
FEATURES             Location/Qualifiers
     source          1..8439
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      102..557
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        584..688
                     /gene="bla"
                     /label=AmpR promoter
     CDS             689..1549
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1720..2308
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     mobile_element  2611..2835
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2902..3435)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(3624..3652)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     polyA_signal    4161..4209
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        complement(4345..4454)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     promoter        4478..4569
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             4612..5265
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     CDS             5272..5289
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             5299..5319
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     misc_feature    5326..7170
                     /label=SINTBAD
     CDS             7183..7896
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     polyA_signal    8029..8163
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  8192..8357
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
       61 ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
      121 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      181 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
      241 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
      301 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
      361 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
      421 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
      481 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
      541 attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      601 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      661 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      721 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
      781 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
      841 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
      901 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
      961 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     1021 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     1081 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     1141 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     1201 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     1261 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     1321 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     1381 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     1441 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     1501 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     1561 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1621 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     1681 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1741 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1801 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1861 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     1921 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     1981 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     2041 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     2101 acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     2161 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     2221 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     2281 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     2341 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     2401 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     2461 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     2521 tctgtgcggt atttcacacc gcagaccagc cgcgtaacct ggcaaaatcg gttacggttg
     2581 agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
     2641 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
     2701 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
     2761 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg
     2821 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc
     2881 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac
     2941 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc
     3001 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagga gattgatgag
     3061 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat
     3121 ctcactacgc ggctgctcaa acctgggcag aacgtaagcc gcgagagcgc caacaaccgc
     3181 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta
     3241 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag
     3301 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat
     3361 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt
     3421 gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
     3481 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga
     3541 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
     3601 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc
     3661 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg
     3721 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg
     3781 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg cccttgcttc
     3841 aggagatcgg tagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag
     3901 tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct
     3961 atagttctag tggttggcct acgtacccgt agtggctatg gcagggcttg ccgccccgac
     4021 gttggctgcg agccctgggc cttcacccga acttgggggt tggggtgggg aaaaggaaga
     4081 aacgcgggcg tattggtccc aatggggtct cggtggggta tcgacagagt gccagccctg
     4141 ggaccgaacc ccgcgtttat gaacaaacga cccaacaccc gtgcgtttta ttctgtcttt
     4201 ttattgccgt catagcgcgg gttccttccg gtattgtctc cttccgtgtt tcagttagcc
     4261 tcccccatct cccggtaccg catgctatgc atcagctgct agcaccatgg ctcgagatcc
     4321 cgggtgatca agtcttcgtc gagtgattgt aaataaaatg taatttacag tatagtattt
     4381 taattaatat acaaatgatt tgataataat tcttatttaa ctataatata ttgtgttggg
     4441 ttgaattaaa ggtccgtata ctccggaata ttaatagatc atggagataa ttaaaatgat
     4501 aaccatctcg caaataaata agtattttac tgttttcgta acagttttgt aataaaaaaa
     4561 cctataaata ttccggatta ttcataccgt cccaccatcg ggcgcggatc catgagtcct
     4621 atcctcggtt actggaaaat caaaggtctg gttcagccca cccgtctcct cctggaatac
     4681 ttggaagaaa aatacgaaga gcacctgtac gagcgcgacg aaggcgacaa gtggcgtaac
     4741 aagaagttcg agctgggact ggaattcccc aacctgccat actacatcga cggcgacgtc
     4801 aagctgaccc agtccatggc tatcatcaga tacatcgccg acaagcacaa catgctgggt
     4861 ggctgcccta aggagcgcgc tgaaatcagc atgctggagg gtgccgtgct ggacatccgt
     4921 tacggcgtct caagaatcgc ttactccaag gacttcgaaa ctctgaaggt cgacttcctg
     4981 agcaagctgc ctgagatgct gaagatgttc gaagaccgtc tgtgccacaa gacctacctg
     5041 aacggagacc acgtgactca ccccgacttc atgctgtacg acgctctgga cgtggtcctg
     5101 tacatggacc caatgtgcct ggacgctttc cctaagctgg tgtgcttcaa gaagcgtatc
     5161 gaggccatcc ctcagatcga caagtacctg aagtccagca agtacatcgc ttggcctctg
     5221 caaggatggc aagctacctt cggaggtggc gaccaccctc ccaagtcaga cctggtcccc
     5281 aggggttccc cagagttcga aaacctgtac ttccagggag gtggcatgga gagcatgttc
     5341 gaagacgaca tctctatcct gactcaggag gctctgggac catctgaagt gtggctggac
     5401 agccctggtg acccctctct gggaggtgac atgtgctccg ccagccactt cgctctgatc
     5461 accgcctacg gcgacatcaa ggagaggctg ggcggactgg agagagaaaa cgccactctg
     5521 cgccgtaggc tgaaggtgta cgagatcaag taccctctga tctcagactt cggcgaggaa
     5581 cacggattca gcctgtacga aatcaaggac ggatctctgc tggaggtgga aaaggtctca
     5641 ctgcagcagc gtctgaacca gttccagcac gagctgcaga agaacaagga acaggaggaa
     5701 cagctgggag agatgatcca ggcttacgag aagctgtgcg tcgaaaagtc cgacctggag
     5761 accgaactga gggagatgag agccctggtg gaaactcacc tgcgccagat ctgcggcctg
     5821 gaacagcagc tgcgtcagca gcagggactg caggacgctg ccttctctaa cctctcacca
     5881 cctcccgctc ctgccccacc ttgcactgac ctggacctgc actacctggc tctgcgcggt
     5941 ggctccggtc tgtctcacgc tggttggcct ggcagcactc cctctgtctc agacctggag
     6001 agacgccgtc tggaggaagc tctggaggct gctcagggtg aagctcgcgg cgctcagctg
     6061 agggaggaac aactccaggc tgagtgcgaa aggctgcagg gagagctgaa gcagctgcag
     6121 gaaacccgtg ctcaggacct ggcctctaac cagtcagaga gggacatggc ttgggtgaag
     6181 agagtcggcg acgaccaggt gaacctggct ctggcctaca ccgagctgac tgaggaactg
     6241 ggtaggctga gagaactgtc ttcactgcag ggccgcatcc tgcgtactct gctgcaggaa
     6301 caggccaggt ccggaggtca aagacactcc cctctgagcc agcgtcactc tcctgctcct
     6361 cagtgcccat ccccatcccc tcctgctagg gctgcccctc cctgcccacc ttgccagtca
     6421 cctgtgcccc agaggagatc cccagtgcct ccttgcccaa gccctcagca gaggcgttct
     6481 ccagcttctc cttcatgccc ctccccagtg cctcagagga gatcaccagt ccctccctct
     6541 tgccagtccc caagcccaca gaggcgtagc cctgtcccac cttcttgccc tgctccccag
     6601 cctcgtcccc cacctccccc acctcccgga gagcgcaccc tggccgaacg tgcttacgcc
     6661 aagccacctt ctcaccacgt gaaggctgga ttccagggta ggagatctta ctcagagctg
     6721 gccgaaggag ctgcctacgc tggtgcttca cccccatggc tgcaggctga ggctgccact
     6781 ctgcctaagc ccagggccta cggttccgaa ctgtacggac ctggtagacc actgagccct
     6841 cgccgtgctt tcgagggtat ccgcctgcgt ttcgaaaagc agccatctga ggaagacgag
     6901 tgggctgtgc ctacctctcc tccctcacca gaagtcggaa ctatcagatg cgcttctttc
     6961 tgcgccggtt tcccaatccc tgaatctcct gctgctactg cttacgctca cgctgaacac
     7021 gctcagtcct ggccaagcat caacctgctg atggagactg tcggcagcga catcaggtct
     7081 tgccctctgt gccagctggg attcccagtg ggttaccctg acgacgccct gatcaagcac
     7141 atcgactcac acctggagaa ctccaagatc ggcggaggtg gcatgtccaa gggcgaggaa
     7201 ctgttcaccg gagtggtccc catcctggtg gaactggacg gcgacgtcaa cggacacaag
     7261 ttctccgtga gcggcgaggg agaaggtgac gctacctacg gcaagctgac tctgaagttc
     7321 atctgcacca ctggcaagct gcccgtgcca tggcctaccc tggtcaccac tctgacttac
     7381 ggtgtccagt gcttctctcg ctaccccgac cacatgaagc agcacgactt cttcaagtca
     7441 gctatgccag agggctacgt gcaggaaagg accatcttct tcaaggacga cggaaactac
     7501 aagaccagag ccgaggtgaa gttcgaagga gacactctgg tcaaccgcat cgagctgaag
     7561 ggtatcgact tcaaggaaga cggtaacatc ctgggccaca agctggagta caactacaac
     7621 tcccacaacg tgtacatcat ggctgacaag cagaagaacg gtatcaaggt caacttcaag
     7681 atccgtcaca acatcgaaga cggcagcgtg cagctggccg accactacca gcagaacact
     7741 cctatcggcg acggacctgt cctgctgcca gacaaccact acctgtctac tcagtcagct
     7801 ctgtccaagg accctaacga gaagcgcgac cacatggtgc tgttggagtt cgttacggct
     7861 gctggaatca cccacggtat ggatgagctc tacaaataaa agcttgtcga gaagtactag
     7921 aggatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
     7981 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
     8041 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     8101 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     8161 atctgatcac tgcttgagcc taggagatcc gaaccagata agtgaaatct agttccaaac
     8221 tattttgtca tttttaattt tcgtattagc ttacgacgct acacccagtt cccatctatt
     8281 ttgtcactct tccctaaata atccttaaaa actccatttc cacccctccc agttcccaac
     8341 tattttgtcc gcccacagcg gggcattttt cttcctgtta tgtttttaat caaacatcct
     8401 gccaactcca tgtgacaaac cgtcatcttc ggctacttt
//
