LOCUS       #AB76_Bxb1-tdTom        7854 bp DNA     circular SYN 04-APR-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7854)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Apr 4, 2023 from SnapGene 6.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7854
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This forward directional feature has 3 segments:
                        1: 143 .. 148 / #ffffff / -35
                        2: 149 .. 166 / #ffffff
                        3: 167 .. 173 / #ffffff / -10"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     186..209
                     /label=M13rev(-48) (#12 & #177)
                     /note="color: black; sequence: AGCGGATAACAATTTCACACAGGA"
     primer_bind     205..221
                     /label=M13rev (#11)
                     /note="color: black; sequence: CAGGAAACAGCTATGAC; added: 
                     2022-09-08"
     promoter        253..463
                     /label=SV40 promoter
                     /note="SV40 early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      322..457
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     primer_bind     complement(420..442)
                     /label=SV40_rev (#RD214)
                     /note="Screening primer 3' to 5' in SV40 promoter"
                     /note="color: black; sequence: CTCCTCACTACTTCTGGAATAGC; 
                     added: 2022-02-09"
     promoter        559..1736
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     complement(1357..1387)
                     /label=tdT_HiFi_Rev
                     /note="color: black; sequence: 
                     CTCCTATACGAAGTTATcgtgaggaagagttcttgcagcaGgcggggaggcggcc; 
                     added: 2022-09-05"
     primer_bind     1530..1552
                     /label=EF1a_qPCRfwd (#195)
                     /note="color: black; sequence: TTTTGGAGTACGTCGTCTTTAGG"
     primer_bind     complement(1621..1643)
                     /label=EF1a_qPCRrev (#196)
                     /note="color: black; sequence: GGAGAATTACATCAAGTGCCAAG"
     primer_bind     1637..1656
                     /label=AB EF1a Fwd
                     /note="#AB19"
                     /note="color: black; sequence: ATTCTCCTTGGAATTTGCCC; added:
                     2022-09-08"
     misc_recomb     1754..1795
                     /label=attB
                     /note="from myobacteriumphage bxb1 (Thomson et al 2012)"
                     /note="color: #33cccc; direction: RIGHT"
     primer_bind     1759..1778
                     /label=attB Fwd
                     /note="#AB03"
                     /note="color: black; sequence: ttgtcgacgacggcggtctc; added:
                     2022-09-08"
     protein_bind    1802..1835
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
                     /note="color: #31849b; direction: RIGHT"
     primer_bind     complement(1836..1860)
                     /label=neo4 (#13)
                     /note="color: black; sequence: CGATGCCTGCTTGCCGAATATCATG"
     primer_bind     complement(1836..1855)
                     /label=AB Neo4 Rev
                     /note="#AB16"
                     /note="color: black; sequence: CCTGCTTGCCGAATATCATG; added:
                     2022-09-08"
     primer_bind     1838..1897
                     /label=pCAG_tdT_HiFi_Fwd
                     /note="color: black; sequence: 
                     tgatattcggcaagcaggcatcgACTAGTTAATTAAGCGTTACATAACTTACGGTAAAT
                     G; added: 2022-09-05"
     primer_bind     1843..1898
                     /label=pCAG_DD_HiFi_Fwd
                     /note="color: black; sequence: 
                     ttcggcaagcaggcatcgACTAGTTAATTAAGTTTGCGTTACATAACTTACGGTAAATG
                     G; added: 2022-08-09"
     misc_feature    1875..2795
                     /label=pCAG
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     complement(1885..1907)
                     /label=CAG_rev (#320)
                     /note="color: black; sequence: CCAGGCGGGCCATTTACCGTAAG; 
                     added: 2019-02-25"
     primer_bind     complement(2081..2100)
                     /label=CAG_rev (#320)
                     /note="color: black; sequence: CCAGGCGGGCCATTTACCGTAAG; 
                     added: 2019-02-25"
     primer_bind     2466..2490
                     /label=CAG_fwd (#321)
                     /note="color: black; sequence: CATTGCCTTTTATGGTAATCGTGCG; 
                     added: 2019-02-25"
     primer_bind     2719..2741
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
                     /note="color: black; sequence: GGTTCGGCTTCTGGCGTGTGACC; 
                     added: 2019-02-25"
     primer_bind     complement(2765..2799)
                     /label=pCAG_DD_HiFi_Rev
                     /note="color: black; sequence: 
                     ggggagatggtttccacctgcactcccatCTGTagGAAAAAGAAGAAGGCATGAACATG
                     G; added: 2022-08-09"
     primer_bind     complement(2770..2815)
                     /label=pCAG_tdT_HiFi_Rev
                     /note="color: black; sequence: 
                     tcctcgcccttgctcaccatCTGTagGAAAAAGAAGAAGGCATGAA; added: 
                     2022-09-05"
     primer_bind     2776..2817
                     /label=tdT_HiFi_Fwd
                     /note="color: black; sequence: 
                     CCTTCTTCTTTTTCctACAGatggtgagcaagggcgaggagg; added: 
                     2022-09-05"
     CDS             2796..4226
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /note="color: #ff0000"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGRYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     primer_bind     2796..2816
                     /label=mCherry_ATG_fwd (#347)
                     /note="color: black; sequence: CATGGTGAGCAAGGGCGAGGAG; 
                     added: 2022-08-09"
     primer_bind     complement(2796..2816)
                     /label=mCherry_ATG_rev (#348)
                     /note="color: black; sequence: CTCCTCGCCCTTGCTCACCAT; 
                     added: 2022-08-09"
     regulatory      2796..2799
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
                     /regulatory_class="other"
     primer_bind     complement(2803..2816)
                     /label=GFPrev2 (#6&#181)
                     /note="color: black; sequence: CCGGTGAACAGCTCCTCGCCCTTGC; 
                     added: 2022-08-09"
     primer_bind     2907..2929
                     /label=mCherry_probe_fwd (#422)
                     /note="5' to 3' primer ~120bp inside mCherry; for 
                     generating Southern probe"
                     /note="color: black; sequence: CCCTACGAGGGCACCCAGACCGC; 
                     added: 2022-08-09"
     primer_bind     2908..2926
                     /label=mCherry_Fwd_1 (#422)
                     /note="color: black; sequence: CCTACGAGGGCACCCAGAC; added: 
                     2022-09-08"
     primer_bind     complement(3084..3103)
                     /label=RFPrev3 (#172)
                     /note="color: black; sequence: CCGTCCTCGAAGTTCATCAC; added:
                     2022-08-09"
     primer_bind     complement(3264..3282)
                     /label=RFP570rev (#256)
                     /note="color: black; sequence: GGATCTCGCCCTTCAGCAC; added: 
                     2022-08-09"
     primer_bind     3406..3428
                     /label=Cherry4 (#389)
                     /note="color: black; sequence: CCCACAACGAGGACTACACCATC; 
                     added: 2022-08-09"
     primer_bind     3633..3655
                     /label=mCherry_probe_fwd (#422)
                     /note="5' to 3' primer ~120bp inside mCherry; for 
                     generating Southern probe"
                     /note="color: black; sequence: CCCTACGAGGGCACCCAGACCGC; 
                     added: 2022-08-09"
     primer_bind     3634..3652
                     /label=mCherry_Fwd_1 (#422)
                     /note="color: black; sequence: CCTACGAGGGCACCCAGAC; added: 
                     2022-09-08"
     primer_bind     complement(3810..3829)
                     /label=RFPrev3 (#172)
                     /note="color: black; sequence: CCGTCCTCGAAGTTCATCAC; added:
                     2022-08-09"
     primer_bind     complement(3990..4008)
                     /label=RFP570rev (#256)
                     /note="color: black; sequence: GGATCTCGCCCTTCAGCAC; added: 
                     2022-08-09"
     primer_bind     4132..4154
                     /label=Cherry4 (#389)
                     /note="color: black; sequence: CCCACAACGAGGACTACACCATC; 
                     added: 2022-08-09"
     primer_bind     4205..4227
                     /label=mCherry_Stop_fwd (#346)
                     /note="color: black; sequence: CATGGACGAGCTGTACAAGTAAG; 
                     added: 2022-08-09"
     primer_bind     complement(4247..4263)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(4248..4264)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
                     /note="color: black; sequence: TCGAGGTCGACGGTATC"
     protein_bind    complement(4275..4299)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
                     /note="color: #31849b; direction: LEFT"
     misc_feature    4330..4918
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     primer_bind     complement(4355..4375)
                     /label=WPRE_rev (#231)
                     /note="color: black; sequence: GAATACCAGTCAATCTTTCAC; 
                     added: 2022-08-09"
     primer_bind     complement(4383..4403)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
                     /note="color: black; sequence: CATAGCGTAAAAGGAGCAACA"
     primer_bind     complement(4388..4414)
                     /label=WPRE_rev2 (#RD450)
                     /note="primer 3' to 5' in WPRE element near 5' end for IVT 
                     reaction"
                     /note="color: black; sequence: cagcgtatccacatagcgtaaaaggag;
                     added: 2022-08-09"
     primer_bind     4806..4825
                     /label=WPRE_Fwd_1
                     /note="color: black; sequence: cctcaatccagcggaccttc; added:
                     2022-09-08"
     primer_bind     complement(4905..4959)
                     /label=tdT_HiFi_Rev
                     /note="color: black; sequence: 
                     CTCCTATACGAAGTTATcgtgaggaagagttcttgcagcaGgcggggaggcggcc; 
                     added: 2022-09-05"
     primer_bind     4921..4942
                     /label=Puro_153fwd (#258)
                     /note="color: black; sequence: GCTGCAAGAACTCTTCCTCACG"
     protein_bind    4943..4975
                     /label=lox251
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites."
                     /note="color: #31849b; direction: RIGHT"
     primer_bind     complement(4983..5000)
                     /label=M13fwd (#10 & #176)
                     /note="color: black; sequence: TGTAAAACGACGGCCAGT"
     primer_bind     complement(4983..4999)
                     /label=M13fwd(-20) (#9)
                     /note="color: black; sequence: GTAAAACGACGGCCAGT; added: 
                     2022-09-08"
     primer_bind     complement(4990..5011)
                     /label=LacZ_rev (#328)
                     /note="color: black; sequence: AGTCACGACGTTGTAAAACGAC"
     primer_bind     complement(5081..5104)
                     /label=pBSKrev (#18)
                     /note="color: black; sequence: GGCGATCGGTGCGGGCCTCTTCGC"
     rep_origin      5212..5667
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        5949..6053
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     complement(5998..6020)
                     /label=NEB_pMini_seqrev (#DM31)
                     /note="For sequencing from the NEB pMini plasmid"
                     /note="color: black; sequence: TCAGGGTTATTGTCTCATGAGCG; 
                     added: 2022-02-09"
     CDS             6054..6914
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 6054 .. 6122 / #ccffcc / signal sequence
                        2: 6123 .. 6914 / #ccffcc
                     Cleavage site after base 6122"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6315..6334)
                     /label=Amp_seq3 (#119)
                     /note="color: black; sequence: TGTATGCGGCGACCGAGTTG"
     primer_bind     complement(6915..6934)
                     /label=Amp_seq1 (#120)
                     /note="color: black; sequence: GAGTAAACTTGGTCTGACAG"
     rep_origin      7085..7673
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     primer_bind     7769..7792
                     /label=ColE1_rev (#118)
                     /note="color: black; sequence: CCGTATTACCGCCTTTGAGTGAGC"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctta
      241 attccctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
      301 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
      361 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
      421 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctttgc
      481 aaagatggat aaagttttaa acagagagga atctttgcag ctaatggacc ttctaggtct
      541 tgaaaggagt gggaattggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag
      601 tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg
      661 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag
      721 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca
      781 gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg cctctttacg ggttatggcc
      841 cttgcgtgcc ttgaattact tccacctggc tgcagtacgt gattcttgat cccgagcttc
      901 gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg
      961 cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc
     1021 gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg
     1081 cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta
     1141 tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg
     1201 cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc
     1261 ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc
     1321 tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag
     1381 ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa
     1441 ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc
     1501 cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg
     1561 aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag
     1621 cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca
     1681 ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgaggaa
     1741 ttcgccacca tggccggctt gtcgacgacg gcggtctccg tcgtcaggat catccggatc
     1801 cataacttcg tatagcatac attatacgaa gttatcatga tattcggcaa gcaggcatcg
     1861 actagttaat taagcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     1921 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     1981 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     2041 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     2101 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     2161 gtcatcgcta ttaccatggt cgaggtgagc cccacgttct gcttcactct ccccatctcc
     2221 cccccctccc cacccccaat tttgtattta tttatttttt aattattttg tgcagcgatg
     2281 ggggcggggg gggggggggc gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg
     2341 ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa agtttccttt
     2401 tatggcgagg cggcggcggc ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc
     2461 gcagccattg ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa
     2521 tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc ctctagcggg cgcggggcga
     2581 agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc
     2641 gccgtcccct tctccctctc cagcctcggg gctgtccgcg gggggacggc tgccttcggg
     2701 ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agagcctctg
     2761 ctaaccatgt tcatgccttc ttctttttcc tacagatggt gagcaagggc gaggaggtca
     2821 tcaaagagtt catgcgcttc aaggtgcgca tggagggctc catgaacggc cacgagttcg
     2881 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg
     2941 tgaccaaggg cggccccctg cccttcgcct gggacatcct gtccccccag ttcatgtacg
     3001 gctccaaggc gtacgtgaag caccccgccg acatccccga ttacaagaag ctgtccttcc
     3061 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggtctg gtgaccgtga
     3121 cccaggactc ctccctgcag gacggcacgc tgatctacaa ggtgaagatg cgcggcacca
     3181 acttcccccc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctccaccg
     3241 agcgcctgta cccccgcgac ggcgtgctga agggcgagat ccaccaggcc ctgaagctga
     3301 aggacggcgg ccactacctg gtggagttca agaccatcta catggccaag aagcccgtgc
     3361 aactgcccgg ctactactac gtggacacca agctggacat cacctcccac aacgaggact
     3421 acaccatcgt ggaacagtac gagcgctccg agggccgcca ccacctgttc ctggggcatg
     3481 gcaccggcag caccggcagc ggcagctccg gcaccgcctc ctccgaggac aacaacatgg
     3541 ccgtcatcaa agagttcatg cgcttcaagg tgcgcatgga gggctccatg aacggccacg
     3601 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc
     3661 tgaaggtgac caagggcggc cccctgccct tcgcctggga catcctgtcc ccccagttca
     3721 tgtacggctc caaggcgtac gtgaagcacc ccgccgacat ccccgattac aagaagctgt
     3781 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggtctggtga
     3841 ccgtgaccca ggactcctcc ctgcaggacg gcacgctgat ctacaaggtg aagatgcgcg
     3901 gcaccaactt cccccccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct
     3961 ccaccgagcg cctgtacccc cgcgacggcg tgctgaaggg cgagatccac caggccctga
     4021 agctgaagga cggcggccgc tacctggtgg agttcaagac catctacatg gccaagaagc
     4081 ccgtgcaact gcccggctac tactacgtgg acaccaagct ggacatcacc tcccacaacg
     4141 aggactacac catcgtggaa cagtacgagc gctccgaggg ccgccaccac ctgttcctgt
     4201 acggcatgga cgagctgtac aagtaagaat tcgatatcaa gcttatcgat accgtcgacc
     4261 tcgagcctct agatacccag ctttcttgta caaagtggtt gatatccagc acagtggcgg
     4321 ccgctcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     4381 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     4441 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     4501 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     4561 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     4621 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     4681 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
     4741 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     4801 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     4861 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
     4921 gctgcaagaa ctcttcctca cgataacttc gtataggaga ctttatacga agttaagcgc
     4981 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     5041 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     5101 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc
     5161 cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct
     5221 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     5281 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     5341 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     5401 ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct
     5461 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     5521 tccaaactgg aacaacactc aaccctatct cgggctattc ttttgattta taagggattt
     5581 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     5641 ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg
     5701 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     5761 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     5821 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     5881 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     5941 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     6001 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     6061 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     6121 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     6181 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     6241 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     6301 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     6361 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     6421 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     6481 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     6541 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     6601 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     6661 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     6721 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     6781 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     6841 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     6901 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     6961 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     7021 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     7081 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     7141 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     7201 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     7261 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     7321 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     7381 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     7441 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     7501 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     7561 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     7621 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     7681 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     7741 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     7801 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaag
//
