LOCUS       pCL040:_lenti_TRE_3xFLA 9956 bp ds-DNA     circular     20-DEC-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1..31
                     /label="MCS"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          6..51
                     /label="oCL025"
                     /note="sequence: aGATCCCATTTGACTCAGgcggccgcGCCACTAGTCGAGagttggc"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     promoter        32..427
                     /label="TRE3G promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    52..560
                     /label="PCR1 (from pLB17)"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     protein_bind    57..75
                     /label="tet operator"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     protein_bind    93..111
                     /label="tet operator"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     protein_bind    129..147
                     /label="tet operator"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     protein_bind    165..183
                     /label="tet operator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind    201..219
                     /label="tet operator"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     protein_bind    237..255
                     /label="tet operator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind    273..291
                     /label="tet operator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          334..353
                     /label="QB0737"
                     /note="sequence: agagctcgtttagtgaaccg"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          335..357
                     /label="oCL097"
                     /note="sequence: gagctcgtttagtgaaccgtcag"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     336..360
                     /label="LNCX"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          complement(423..482)
                     /label="oCL068"
                     /note="sequence: gtcatgatctttataatcaccgtcatggtctttgtagtcCATGGTGGCGACCGGTtttac"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    428..443
                     /label="Kozak"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             441..563
                     /label="Translation 441-563"
                     /translation="MDYKDHDGDYKDHDIDYKDDDDKGGSGSGSGDGSSVSGSG*"
     misc_feature    444..509
                     /label="3XFLAG"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          455..480
                     /label="oCL069"
                     /note="sequence: ccatgacggtgattataaagatcatg"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(455..514)
                     /label="oCL070"
                     /note="sequence: ccACCcttgtcatcgtcatccttgtagtcgatgtcatgatctttataatcaccgtcatgg"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          489..511
                     /label="oCL012"
                     /note="sequence: tacaaggatgacgatgacaagGG"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(494..553)
                     /label="oCL071"
                     /note="sequence: ccaGAGACGcTtgaCCCGTCTCcggagccTGAtccGGATccACCcttgtcatcgtcatcc"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          512..530
                     /label="HTrec F bind"
                     /note="sequence: tggATCCggaTCAggctcc"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    513..530
                     /label="Linker"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    531..551
                     /label="TypeIIS cloning site"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(534..590)
                     /label="oCL072"
                     /note="sequence: gtgggcgatcgattcgcgagtttaaacTTATCcagaTccaGAGACGcTtgaCCCGTC"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(541..590)
                     /label="oCL026"
                     /note="sequence: gtgggcgatcgattcgcgagtttaaacTTATCcagaTccaGAGACGcTtg"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    552..560
                     /label="Linker"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(552..581)
                     /label="HT-Induce Design 1 anneal"
                     /note="sequence: cgattcgcgagtttaaacTTATCcagaTcc"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(571..588)
                     /label="oCL098"
                     /note="sequence: gggcgatcgattcgcgag"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          571..618
                     /label="oCL027"
                     /note="sequence: ctcgcgaatcgatcgcccaccccaacttgtttattgcagcttataatg"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     polyA_signal    594..715
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     complement(631..650)
                     /label="SV40pA-R"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer_bind     685..704
                     /label="EBV-rev"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          685..704
                     /label="QB0017"
                     /note="sequence: gtggtttgtccaaactcatc"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          complement(692..743)
                     /label="oCL028"
                     /note="sequence: ccctcataaaagttttgttacttatgcataagatacattgatgagtttggac"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          721..749
                     /label="oCL029"
                     /note="sequence: aagtaacaaaacttttatgagggacagcc"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          721..749
                     /label="MH011 binding"
                     /note="sequence: aagtaacaaaacttttatgagggacagcc"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    721..996
                     /label="from pMH003"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     enhancer        739..970
                     /label="core insul"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(974..996)
                     /label="MH012 binding"
                     /note="sequence: gcataatactagtggccgtggag"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(974..996)
                     /label="oCL030"
                     /note="sequence: gcataatactagtggccgtggag"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          977..1015
                     /label="oCL031"
                     /note="sequence: cacggccactagtattatgcatGTGCCCGTCAGTGGGCA"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    999..1960
                     /label="EF1Alpha"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1962..2171
                     /label="EF1Alpha"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          2119..2138
                     /label="QB2748"
                     /note="sequence: CAAGCCTCAGACAGTGGTTC"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             2181..4112
                     /label="Translation 2181-4112"
                     /translation="MSRLDKSKIINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHVKNKRALLDALPIEMLDRHHTHSCPLEPESWQDFLRNNAKSYRCALLSHRDGAKVHLGTRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTTDSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPADALDDFDLDMLPADALDDFDLDMLPGGSGEGRGSLLTCGDVEENPGPMVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYKGSGSGSMAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGVNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIKAIVKDSDGQPTAVGIRELLPSGYVWEG*"
     misc_feature    2184..2801
                     /label="rTetR(SE-G72P)"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          2668..2689
                     /label="JT836"
                     /note="sequence: ccgattctatgcccccacttct"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(2780..2798)
                     /label="JT827"
                     /note="sequence: gccgctttcgcactttagc"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(2784..2843)
                     /label="oCL032"
                     /note="sequence: gggcagcatgtccaggtcgaagtcgtccagggcgtcggtgggaccgccgctttcgcactt"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    2802..2924
                     /label="VP48"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(2826..2885)
                     /label="oCL033"
                     /note="sequence: agcagggagcatatccagatcaaaatcatccagggcatcggcgggcagcatgtccaggtc"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(2864..2923)
                     /label="oCL034"
                     /note="sequence: cctggcagcatatcgagatcgaagtcatcgagagcgtcagcagggagcatatccagatca"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          2903..2948
                     /label="oCL035"
                     /note="sequence: cgatctcgatatgctgccaggcggAtctgGAgagggAaggggAagc"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    2925..2933
                     /label="Linker"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(2925..2944)
                     /label="HTrec R bind site"
                     /note="sequence: cccctTccctcTCcagaTcc"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2934..2987
                     /label="T2A"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(2969..2987)
                     /label="JT809"
                     /note="sequence: tgggccaggattctcctcg"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             2988..3695
                     /label="mCherry"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     primer          complement(3069..3089)
                     /label="QB4520"
                     /note="sequence: gatctcgaactcgtggccgtt"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3696..3713
                     /label="Linker"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             3714..4112
                     /label="BSD"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(4093..4137)
                     /label="oCL036"
                     /note="sequence: agttattaggtccctcgacgaattcttagccctcccacacataac"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_recomb     4132..4165
                     /label="LoxP recombination site"
                     /ApEinfo_revcolor="#4682b4"
                     /ApEinfo_fwdcolor="#4682b4"
     misc_feature    4211..4811
                     /label="wPRE"
                     /ApEinfo_revcolor="#0060ff"
                     /ApEinfo_fwdcolor="#0060ff"
     primer          complement(4217..4238)
                     /label="QB2745"
                     /note="sequence: gtaatccagaggttgattatcg"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    5001..5015
                     /label="cPPT"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    5338..5518
                     /label="HIV-1_5_LTR"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     rep_origin      complement(5562..6244)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_fwdcolor="#808080"
     misc_feature    complement(5565..6184)
                     /label="pBR322_origin"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(5589..5598)
                     /label="GC Box"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             complement(6342..7001)
                     /label="AmpR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    complement(7241..7269)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    7595..8158
                     /label="pU6"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    complement(8098..8107)
                     /label="GC Box"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    8176..8356
                     /label="HIV-1_5_LTR"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    8403..8528
                     /label="HIV-1 Ψ"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    8529..8870
                     /label="backbone shared with pWJ037"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    8878..9026
                     /label="backbone shared with pWJ037"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    complement(8933..8939)
                     /label="TATA box"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    9027..9260
                     /label="RRE"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    9791..9805
                     /label="cPPT"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          9820..9841
                     /label="QB2741"
                     /note="sequence: tgcaggggaaagaatagtagac"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
ORIGIN
        1 tctagaGATC CCATTTGACT CAGgcggccg cGCCACTAGT CGAGagttgg ctttactccc
       61 tatcagtgat agagaacgta tgaagagttt actccctatc agtgatagag aacgtatgca
      121 gactttactc cctatcagtg atagagaacg tataaggagt ttactcccta tcagtgatag
      181 agaacgtatg accagtttac tccctatcag tgatagagaa cgtatctaca gtttactccc
      241 tatcagtgat agagaacgta tatccagttt actccctatc agtgatagag aacgtataag
      301 ctttaggcgt gtacggtggg cgcctataaa agcagagctc gtttagtgaa ccgtcagatc
      361 gcctggagca attccacaac acttttgtct tataccaact ttccgtacca cttcctaccc
      421 tcgtaaaACC GGTCGCCACC ATGgactaca aagaccatga cggtgattat aaagatcatg
      481 acatcgacta caaggatgac gatgacaagG GTggATCCgg aTCAggctcc gGAGACGGGt
      541 caAgCGTCTC tggAtctgGA TAAgtttaaa ctcgcgaatc gatcgcccac cccaacttgt
      601 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
      661 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatgcat
      721 aagtaacaaa acttttatga gggacagccc ccccccaaag cccccaggga tgtaattacg
      781 tccctccccc gctagggggc agcagcgagc cgcccggggc tccgctccgg tccggcgctc
      841 cccccgcatc cccgagccgg cagcgtgcgg ggacagcccg ggcacgggga aggtggcacg
      901 ggatcgcttt cctctgaacg cttctcgctg ctctttgagc ctgcagacac ctggggggat
      961 acggggaaaa ggcctccacg gccactagta ttatgcatGT GCCCGTCAGT GGGCAGAGCG
     1021 CACATCGCCC ACAGTCCCCG AGAAGTTGGG GGGAGGGGTC GGCAATTGAA CCGGTGCCTA
     1081 GAGAAGGTGG CGCGGGGTAA ACTGGGAAAG TGATGTCGTG TACTGGCTCC GCCTTTTTCC
     1141 CGAGGGTGGG GGAGAACCGT ATATAAGTGC AGTAGTCGCC GTGAACGTTC TTTTTCGCAA
     1201 CGGGTTTGCC GCCAGAACAC AGGTAAGTGC CGTGTGTGGT TCCCGCGGGC CTGGCCTCTT
     1261 TACGGGTTAT GGCCCTTGCG TGCCTTGAAT TACTTCCACC TGGCTGCAGT ACGTGATTCT
     1321 TGATCCCGAG CTTCGGGTTG GAAGTGGGTG GGAGAGTTCG AGGCCTTGCG CTTAAGGAGC
     1381 CCCTTCGCCT CGTGCTTGAG TTGAGGCCTG GCCTGGGCGC TGGGGCCGCC GCGTGCGAAT
     1441 CTGGTGGCAC CTTCGCGCCT GTCTCGCTGC TTTCGATAAG TCTCTAGCCA TTTAAAATTT
     1501 TTGATGACCT GCTGCGACGC TTTTTTTCTG GCAAGATAGT CTTGTAAATG CGGGCCAAGA
     1561 TCTGCACACT GGTATTTCGG TTTTTGGGGC CGCGGGCGGC GACGGGGCCC GTGCGTCCCA
     1621 GCGCACATGT TCGGCGAGGC GGGGCCTGCG AGCGCGGCCA CCGAGAATCG GACGGGGGTA
     1681 GTCTCAAGCT GGCCGGCCTG CTCTGGTGCC TGGCCTCGCG CCGCCGTGTA TCGCCCCGCC
     1741 CTGGGCGGCA AGGCTGGCCC GGTCGGCACC AGTTGCGTGA GCGGAAAGAT GGCCGCTTCC
     1801 CGGCCCTGCT GCAGGGAGCT CAAAATGGAG GACGCGGCGC TCGGGAGAGC GGGCGGGTGA
     1861 GTCACCCACA CAAAGGAAAA GGGCCTTTCC GTCCTCAGCC GTCGCTTCAT GTGACTCCAC
     1921 GGAGTACCGG GCGCCGTCCA GGCACCTCGA TTAGTTCTCG cGCTTTTGGA GTACGTCGTC
     1981 TTTAGGTTGG GGGGAGGGGT TTTATGCGAT GGAGTTTCCC CACACTGAGT GGGTGGAGAC
     2041 TGAAGTTAGG CCAGCTTGGC ACTTGATGTA ATTCTCCTTG GAATTTGCCC TTTTTGAGTT
     2101 TGGATCTTGG TTCATTCTCA AGCCTCAGAC AGTGGTTCAA AGTTTTTTTC TTCCATTTCA
     2161 GGTGTCGTGA gCTAgccacc ATGtctagac tggacaagag caaaATAata aacggagctc
     2221 tggaattact caatggtgtc ggtatcgaag gcctgacgac aaggaaactc gctcaaaagc
     2281 tgggagttga gcagcctacc ctgtactggc acgtgaagaa caagcgggcc ctgctcgatg
     2341 ccctgccaat cgagatgctg gacaggcatc atacccacTC Ctgccccctg gaaCCCgagt
     2401 catggcaaga ctttctgcgg aacaacgcca agtcataccg ctgtgctctc ctctcacatc
     2461 gcgacggggc taaagtgcat ctcggcaccc gcccaacaga gaaacagtac gaaaccctgg
     2521 aaaatcagct cgcgttcctg tgtcagcaag gcttctccct ggagaacgca ctgtacgctc
     2581 tgtccgccgt gggccacttt acactgggct gcgtattgga ggaacaggag catcaagtag
     2641 caaaagagga aagagagaca cctaccaccg attctatgcc cccacttctg AAAcaagcaa
     2701 ttgagctgtt cgaccggcag ggagccgaac ctgccttcct tttcggcctg gaactaatca
     2761 tatgtggcct ggagaaacag ctaaagtgcg aaagcggcgg tcccaccgac gccctggacg
     2821 acttcgacct ggacatgctg cccgccgatg ccctggatga ttttgatctg gatatgctcc
     2881 ctgctgacgc tctcgatgac ttcgatctcg atatgctgcc aggcggAtct gGAgagggAa
     2941 ggggAagcct cctaacttgc ggagaTgtcg aggagaatcc tggcccaatg gtgagcaagg
     3001 gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg
     3061 gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
     3121 gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca
     3181 tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc
     3241 ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg
     3301 aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct
     3361 acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga
     3421 ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg
     3481 agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca
     3541 cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg
     3601 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc
     3661 gccactccac cggcggcatg gacgagctgt acaagggtag cggcagcggt agcatggcca
     3721 agcctttgtc tcaagaagaa tccaccctca ttgaaagagc aacggctaca atcaacagca
     3781 tccccatctc tgaagactac agcgtcgcca gcgcagctct ctctagcgac ggccgcatct
     3841 tcactggtgt caatgtatat cattttactg ggggaccttg tgcagaactc gtggtgctgg
     3901 gcactgctgc tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga
     3961 acaggggcat cttgagcccc tgcggacggt gccgacaggt gcttctcgat ctgcatcctg
     4021 ggatcaaagc catagtgaag gacagtgatg gacagccgac ggcagttggg attcgtgaat
     4081 tgctgccctc tggttatgtg tgggagggct aagaattcgt cgagggacct aataacttcg
     4141 tatagcatac attatacgaa gttatacatg tttaagggtt ccggttccac taggtacaat
     4201 tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg
     4261 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     4321 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     4381 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     4441 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     4501 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     4561 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     4621 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     4681 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     4741 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     4801 cctccccgca tcgataccgt cgacctcgat cgagacctag aaaaacatgg agcaatcaca
     4861 agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag
     4921 gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct
     4981 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     5041 cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt ccctgattgg
     5101 cagaactaca caccagggcc agggatcaga tatccactga cctttggatg gtgctacaag
     5161 ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa cacccgcttg
     5221 ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtatt agagtggagg
     5281 tttgacagcc gcctagcatt tcatcacatg gcccgagagc tgcatccgga ctgtactggg
     5341 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     5401 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     5461 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagc
     5521 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     5581 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     5641 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     5701 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     5761 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     5821 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     5881 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     5941 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     6001 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     6061 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     6121 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     6181 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     6241 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     6301 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     6361 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     6421 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     6481 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     6541 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     6601 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     6661 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     6721 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     6781 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     6841 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     6901 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     6961 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     7021 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     7081 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     7141 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     7201 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     7261 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     7321 gtgccacctg acgcttaagc ggtcgacgga tcgggagatc tcccgatccc ctatggtgca
     7381 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg
     7441 tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga
     7501 ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac
     7561 gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
     7621 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
     7681 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     7741 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
     7801 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
     7861 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     7921 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
     7981 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
     8041 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
     8101 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagcg
     8161 cgttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     8221 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     8281 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     8341 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
     8401 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
     8461 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
     8521 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
     8581 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
     8641 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
     8701 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
     8761 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
     8821 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc ggccgcgctg
     8881 atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat
     8941 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
     9001 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
     9061 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
     9121 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
     9181 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac
     9241 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
     9301 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg
     9361 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
     9421 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg
     9481 gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa attattcata
     9541 atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat
     9601 agagttaggc agggatattc accattatcg tttcagaccc acctcccaac cccgagggga
     9661 cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt
     9721 cgattagtga acggatcggc actgcgtgcg ccaattctgc agacaaatgg cagtattcat
     9781 ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     9841 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
     9901 ttttcgggtt tattacaggg acagcagaga tccagtttgg ttagtaccgg gcccgc
//