LOCUS       SMC1874_pFastBac        8598 bp DNA     circular SYN 11-JUL-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8598)
  AUTHORS   eliasa55
  TITLE     Direct Submission
  JOURNAL   Exported Feb 7, 2023 from SnapGene 6.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8598
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      102..557
                     /label=M13 origin
     rep_origin      119..425
                     /label=F1 ori
     primer_bind     583..603
                     /label=SMP646
     primer_bind     complement(583..603)
                     /label=SMP654
     primer_bind     complement(734..753)
                     /label=AmpStart
     CDS             887..1546
                     /label=AmpR
     primer_bind     1480..1499
                     /label=AmpStop
     rep_origin      1644..2326
                     /label=ColE1 origin
     primer_bind     complement(3803..3815)
                     /label=SMP2433
     primer_bind     4221..4241
                     /label=SMP2102
     primer_bind     complement(4368..4392)
                     /label=SMP2103
     primer_bind     4494..4511
                     /label=SMP3302
     misc_feature    4528..4549
                     /label=148 Pph_seqF
     primer_bind     complement(4528..4549)
                     /label=SMP2187
     primer_bind     4528..4549
                     /label=SMP3298
     primer_bind     4558..4589
                     /label=SMP3
     misc_feature    4612..5274
                     /label=GST
     primer_bind     4612..4630
                     /label=SMP2582
     primer_bind     complement(4658..4675)
                     /label=SMP2192
     primer_bind     complement(4914..4937)
                     /label=SMP1669
     primer_bind     5153..5174
                     /label=SMP358
     primer_bind     5198..5217
                     /label=GEX5-for
     primer_bind     5229..5246
                     /label=pETG-for
     primer_bind     5230..5249
                     /label=SMP3299
     primer_bind     complement(5248..5274)
                     /label=SMP2863
     misc_feature    5275..5286
                     /label=GST
                     /label=GST(1)
     primer_bind     5301..5346
                     /label=SMP3233
     primer_bind     5301..5346
                     /label=SMP3581
     primer_bind     5301..5346
                     /label=SMP3583
     misc_feature    5302..5322
                     /label=TEV cleavage ENLYFQ^G
     CDS             5329..6045
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     5329..5348
                     /label=SMP2235
     primer_bind     5329..5348
                     /label=SMP3364
     primer_bind     5332..5349
                     /label=SMP2824
     primer_bind     complement(5332..5349)
                     /label=SMP3729
     primer_bind     5335..5352
                     /label=SMP2016
     primer_bind     complement(5371..5387)
                     /label=PEN1-737R
     primer_bind     complement(5374..5395)
                     /label=SMP3301
     primer_bind     complement(5376..5396)
                     /label=SMP919
     primer_bind     complement(5391..5409)
                     /label=SMP2564
     primer_bind     complement(5402..5422)
                     /label=SMP2562
     primer_bind     complement(5404..5422)
                     /label=EGFP-N-R+33
     primer_bind     complement(5437..5455)
                     /label=EGFP-N-rev
     primer_bind     complement(5445..5464)
                     /label=SMP2433
     primer_bind     complement(5460..5478)
                     /label=SMP3388
     primer_bind     5503..5523
                     /label=SMP2316
     primer_bind     5577..5596
                     /label=SMP1001
     primer_bind     5623..5642
                     /label=SMP1325
     primer_bind     complement(5633..5653)
                     /label=SMP2969
     primer_bind     complement(5690..5710)
                     /label=SMP2971
     primer_bind     complement(5693..5709)
                     /label=SMP2578
     primer_bind     complement(5723..5736)
                     /label=SMP2969
     primer_bind     5736..5754
                     /label=SMP444
     primer_bind     5746..5770
                     /label=SMP1666
     primer_bind     5800..5817
                     /label=SMP3040
     primer_bind     5800..5817
                     /label=SMP3104
     primer_bind     5805..5824
                     /label=SMP1330
     primer_bind     5832..5851
                     /label=SMP437
     primer_bind     complement(5832..5851)
                     /label=SMP471
     primer_bind     5883..5902
                     /label=SMP364
     primer_bind     5932..5964
                     /label=SMP295
     primer_bind     complement(5932..5964)
                     /label=SMP296
     primer_bind     5955..5973
                     /label=EGFP-C-F-31
     primer_bind     5964..5980
                     /label=EGFP-for
     primer_bind     5970..5988
                     /label=SMP564
     primer_bind     5982..6003
                     /label=SMP3300
     primer_bind     5986..6003
                     /label=EGFP-C-for
     primer_bind     complement(6018..6036)
                     /label=SMP2415
     primer_bind     complement(6025..6045)
                     /label=SMP2429
     primer_bind     6025..6045
                     /label=SMP3728
     primer_bind     complement(6025..6044)
                     /label=SMP2596
     primer_bind     6028..6078
                     /label=SMP3379
     primer_bind     complement(6028..6078)
                     /label=SMP3380
     primer_bind     6028..6061
                     /label=SMP3381
     primer_bind     complement(6028..6061)
                     /label=SMP3382
     primer_bind     complement(6044..6065)
                     /label=SMP3234
     misc_feature    6046..6057
                     /label=4xGly-linker
     primer_bind     6050..6079
                     /label=SMP3231
     CDS             6058..8058
                     /codon_start=1
                     /label=TBK1-CDS aa1-666
                     /translation="MQSTSNHLWLLSDILGQGATANVFRGRHKKTGDLFAIKVFNNISF
                     LRPVDVQMREFEVLKKLNHKNIVKLFAIEEETTTRHKVLIMEFCPCGSLYTVLEEPSNA
                     YGLPESEFLIVLRDVVGGMNHLRENGIVHRDIKPGNIMRVIGEDGQSVYKLTDFGAARE
                     LEDDEQFVSLYGTEEYLHPDMYERAVLRKDHQKKYGATVDLWSIGVTFYHAATGSLPFR
                     PFEGPRRNKEVMYKIITGKPSGAISGVQKAENGPIDWSGDMPVSCSLSRGLQVLLTPVL
                     ANILEADQEKCWGFDQFFAETSDILHRMVIHVFSLQQMTAHKIYIHSYNTATIFHELVY
                     KQTKIISSNQELIYEGRRLVLEPGRLAQHFPKTTEENPIFVVSREPLNTIGLIYEKISL
                     PKVHPRYDLDGDASMAKAITGVVCYACRIASTLLLYQELMRKGIRWLIELIKDDYNETV
                     HKKTEVVITLDFCIRNIEKTVKVYEKLMKINLEAAELGEISDIHTKLLRLSSSQGTIET
                     SLQDIDSRLSPGGSLADAWAHQEGTHPKDRNVEKLQVLLNCMTEIYYQFKKDKAERRLA
                     YNEEQIHKFDKQKLYYHATKAMTHFTDECVKKYEAFLNKSEEWIRKMLHLRKQLLSLTN
                     QCFDIEEEVSKYQEYTNELQETLPQKMFTAS"
     primer_bind     6649..6668
                     /label=SMP3289
     primer_bind     complement(6731..6750)
                     /label=SMP3607
     primer_bind     7268..7287
                     /label=SMP3290
     primer_bind     7488..7507
                     /label=SMP3291
     misc_feature    complement(8023..8063)
                     /label=rev
     misc_feature    8047..8091
                     /label=for
     CDS             8053..8055
                     /codon_start=1
                     /label=S666
                     /translation="S"
     primer_bind     8059..8080
                     /label=SMP2231
     primer_bind     8059..8079
                     /label=SMP3275
     misc_feature    8093..8113
                     /label=4 SV40_seqR
     primer_bind     complement(8094..8113)
                     /label=SV40-pA-rev
     polyA_signal    complement(8127..8318)
                     /label=SV40 late polyA
     primer_bind     complement(8129..8160)
                     /label=SMP4
     primer_bind     complement(8138..8155)
                     /label=EGFP-C1-anti
     primer_bind     complement(8148..8169)
                     /label=FastBac-rev
     primer_bind     complement(8157..8179)
                     /label=SMP565
     primer_bind     complement(8188..8205)
                     /label=EGFP-C-Rev
     primer_bind     8225..8246
                     /label=pCI-rev
     primer_bind     complement(8229..8248)
                     /label=SMP3314
     primer_bind     8279..8298
                     /label=EBV-rev
ORIGIN
        1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
       61 ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
      121 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      181 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
      241 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
      301 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
      361 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
      421 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
      481 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
      541 attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      601 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      661 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      721 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
      781 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
      841 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
      901 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
      961 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     1021 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     1081 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     1141 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     1201 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     1261 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     1321 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     1381 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     1441 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     1501 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     1561 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1621 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     1681 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1741 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1801 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1861 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     1921 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     1981 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     2041 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     2101 acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     2161 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     2221 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     2281 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     2341 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     2401 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     2461 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     2521 tctgtgcggt atttcacacc gcagaccagc cgcgtaacct ggcaaaatcg gttacggttg
     2581 agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
     2641 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
     2701 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
     2761 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg
     2821 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc
     2881 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac
     2941 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc
     3001 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagga gattgatgag
     3061 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat
     3121 ctcactacgc ggctgctcaa acctgggcag aacgtaagcc gcgagagcgc caacaaccgc
     3181 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta
     3241 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag
     3301 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat
     3361 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt
     3421 gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
     3481 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga
     3541 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
     3601 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc
     3661 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg
     3721 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg
     3781 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg ccctggcttc
     3841 aggagatcgg tagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag
     3901 tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct
     3961 atagttctag tggttggcct acgtacccgt agtggctatg gcagggcttg ccgccccgac
     4021 gttggctgcg agccctgggc cttcacccga acttgggggt tggggtgggg aaaaggaaga
     4081 aacgcgggcg tattggtccc aatggggtct cggtggggta tcgacagagt gccagccctg
     4141 ggaccgaacc ccgcgtttat gaacaaacga cccaacaccc gtgcgtttta ttctgtcttt
     4201 ttattgccgt catagcgcgg gttccttccg gtattgtctc cttccgtgtt tcagttagcc
     4261 tcccccatct cccggtaccg catgctatgc atcagctgct agcaccatgg ctcgagatcc
     4321 cgggtgatca agtcttcgtc gagtgattgt aaataaaatg taatttacag tatagtattt
     4381 taattaatat acaaatgatt tgataataat tcttatttaa ctataatata ttgtgttggg
     4441 ttgaattaaa ggtccgtata ctccggaata ttaatagatc atggagataa ttaaaatgat
     4501 aaccatctcg caaataaata agtattttac tgttttcgta acagttttgt aataaaaaaa
     4561 cctataaata ttccggatta ttcataccgt cccaccatcg ggcgcggatc catggcatcc
     4621 cctatactag gttattggaa aattaagggc cttgtgcaac ccactcgact tcttttggaa
     4681 tatcttgaag aaaaatatga agagcatttg tatgagcgcg atgaaggtga taaatggcga
     4741 aacaaaaagt ttgaattggg tttggagttt cccaatcttc cttattatat tgatggtgat
     4801 gttaaattaa cacagtctat ggccatcata cgttatatag ctgacaagca caacatgttg
     4861 ggtggttgtc caaaagagcg tgcagagatt tcaatgcttg aaggagcggt tttggatatt
     4921 agatacggtg tttcgagaat tgcatatagt aaagactttg aaactctcaa agttgatttt
     4981 cttagcaagc tacctgaaat gctgaaaatg ttcgaagatc gtttatgtca taaaacatat
     5041 ttaaatggtg atcatgtaac ccatcctgac ttcatgttgt atgacgctct tgatgttgtt
     5101 ttatacatgg acccaatgtg cctggatgcg ttcccaaaat tagtttgttt taaaaaacgt
     5161 attgaagcta tcccacaaat tgataagtac ttgaaatcca gcaagtatat agcatggcct
     5221 ttgcagggct ggcaagccac gtttggtggt ggcgaccatc ctccaaaatc ggatctggtt
     5281 ccgcgtggat caggatcagg agagaattta tactttcagg gagcgcgcat ggtgagcaag
     5341 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     5401 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     5461 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     5521 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     5581 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     5641 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     5701 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     5761 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     5821 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     5881 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     5941 cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     6001 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagggcgg tggaggcatg
     6061 caatccacat ccaaccacct ctggctcctc tcggacattc tcggacaagg cgctaccgct
     6121 aacgtgttca ggggaaggca caagaagacc ggtgacctgt tcgccatcaa ggtgttcaac
     6181 aacatctcat tcctgcgccc tgtggacgtc cagatgcgtg agttcgaagt gctgaagaag
     6241 ctgaaccaca agaacatcgt caagctgttc gccatcgagg aagaaaccac tacccgccac
     6301 aaggtgctga tcatggagtt ctgcccctgc ggttccctgt acactgtcct ggaagagcct
     6361 tccaacgctt acggtctgcc cgagagcgag ttcctgatcg tcctgcgtga cgtggtcggt
     6421 ggcatgaacc acctgaggga aaacggcatc gtgcacagag acatcaagcc aggaaacatc
     6481 atgcgcgtga tcggcgagga cggacagagc gtctacaagc tgaccgactt cggcgctgcc
     6541 agagagctgg aagacgacga acagttcgtc tctctgtacg gaactgaaga gtacctgcac
     6601 cctgacatgt acgagagggc cgtgctgaga aaggaccacc agaagaagta cggtgctacc
     6661 gtggacctgt ggtctatcgg cgtcaccttc taccacgctg ccactggatc actgcctttc
     6721 cgccccttcg aaggtcctcg ccgtaacaag gaggtcatgt acaagatcat cactggcaag
     6781 ccctctggcg ccatctcagg agtccagaag gctgaaaacg gtcccatcga ctggagcggc
     6841 gacatgccag tgagctgctc tctgtcccgc ggtctgcaag tgctgctgac cccagtcctg
     6901 gccaacatcc tggaagctga ccaggagaag tgctggggtt tcgaccagtt cttcgctgaa
     6961 acctccgaca tcctgcacag aatggtcatc cacgtcttca gcctgcaaca gatgactgcc
     7021 cacaagattt acatccactc ttacaacact gctactatct tccacgaact ggtctacaag
     7081 cagaccaaga tcatctccag caaccaggaa ctgatctacg agggaaggag actggtgctg
     7141 gagcccggtc gcctggctca gcacttccca aagactaccg aagagaaccc tatcttcgtg
     7201 gtgtcccgcg agccactgaa cactatcggc ctgatctacg agaagatcag cctgcccaag
     7261 gtccacccac gttacgacct ggacggcgac gctagcatgg ctaaggccat caccggagtg
     7321 gtctgctacg cttgccgcat cgcttcaact ctgctgctgt accaggaact gatgcgcaag
     7381 ggaatccgtt ggctgatcga gctgatcaag gacgactaca acgaaactgt gcacaagaag
     7441 accgaggtgg tcatcactct ggacttctgc atccgtaaca tcgaaaagac cgtgaaggtc
     7501 tacgagaagc tgatgaagat caacctggag gctgccgaac tgggagagat cagcgacatc
     7561 cacactaagc tgctgcgcct gtcttcatcc cagggcacta tcgaaacctc cctgcaagac
     7621 atcgactccc gtctgagccc aggaggttca ctggctgacg cttgggctca ccaggaaggc
     7681 acccacccta aggacaggaa cgtggagaag ctgcaagtcc tgctgaactg catgactgaa
     7741 atctactacc agttcaagaa ggacaaggcc gagcgccgtc tggcttacaa cgaagagcag
     7801 atccacaagt tcgacaagca gaagctgtac taccacgcca ccaaggctat gactcacttc
     7861 accgacgaat gcgtcaagaa gtacgaggct ttcctgaaca agagcgaaga gtggatcagg
     7921 aagatgctgc acctgagaaa gcagctgctg tccctgacca accagtgctt cgacatcgaa
     7981 gaggaagtga gcaagtacca ggagtacacc aacgagctgc aagagactct gcctcagaag
     8041 atgttcactg ccagctaaaa gcttgtcgag aagtactaga ggatcataat cagccatacc
     8101 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
     8161 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     8221 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     8281 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tctgatcact gcttgagcct
     8341 aggagatccg aaccagataa gtgaaatcta gttccaaact attttgtcat ttttaatttt
     8401 cgtattagct tacgacgcta cacccagttc ccatctattt tgtcactctt ccctaaataa
     8461 tccttaaaaa ctccatttcc acccctccca gttcccaact attttgtccg cccacagcgg
     8521 ggcatttttc ttcctgttat gtttttaatc aaacatcctg ccaactccat gtgacaaacc
     8581 gtcatcttcg gctacttt
//
