LOCUS       Exported               11548 bp DNA     circular SYN 03-FEB-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11548)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 3, 2023 from SnapGene Viewer 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11548
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    832..929
                     /label=R
                     /note="color: #a6acb3"
     misc_feature    930..1011
                     /label=U5
                     /note="color: #a6acb3"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    1677..1910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
     misc_feature    2413..2530
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /note="color: #ffe4c4"
     promoter        2572..2886
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             2909..4228
                     /codon_start=1
                     /product="human c-Myc proto-oncogene"
                     /label=c-Myc
                     /note="color: #993366"
                     /translation="MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAP
                     SEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTE
                     LLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPN
                     PARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDS
                     LLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGS
                     PSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNR
                     KCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKA
                     TAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA"
     promoter        4246..5455
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             5472..6218
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /note="This feature has 8 segments:
                        1: 5472 .. 6089 / #993366 / rTetR
                        2: 6090 .. 6095 / #cc99b2
                        3: 6096 .. 6131 / #993366 / VP16 minimal transcriptio...
                        4: 6132 .. 6134 / #cc99b2
                        5: 6135 .. 6170 / #993366 / VP16 minimal transcriptio...
                        6: 6171 .. 6173 / #cc99b2
                        7: 6174 .. 6209 / #993366 / VP16 minimal transcriptio...
                        8: 6210 .. 6218 / #cc99b2"
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    6242..6830
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     misc_feature    7029..7348
                     /label=dU3
                     /note="color: #a6acb3"
     misc_feature    7349..7446
                     /label=R
                     /note="color: #a6acb3"
     misc_feature    7447..7528
                     /label=U5
                     /note="color: #a6acb3"
     polyA_signal    7561..7785
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      7831..8259
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        8273..8603
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      8454..8589
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     promoter        8651..8698
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
                     /note="color: #ffffff; direction: RIGHT"
     CDS             8717..9091
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /note="color: #ccffcc"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9221..9342
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      complement(9793..10381)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(10552..11412)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 10552 .. 11343 / #ccffcc
                        2: 11344 .. 11412 / #ccffcc / signal sequence
                     Cleavage site after base 11343"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11413..11517)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     1561 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1621 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1681 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1741 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1801 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1861 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1921 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1981 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     2041 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     2101 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     2161 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     2221 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     2281 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     2341 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctac aaatggcagt
     2401 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     2461 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
     2521 tcaaaatttt cgggtttatt acagggacag cagaaattca ctttgaattg cgagtttact
     2581 ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgatg
     2641 tcgagtttac tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat
     2701 agagaacgta tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttatcc
     2761 ctatcagtga tagagaacgt atgtcgagtt tactccctat cagtgataga gaacgtatgt
     2821 cgaggtaggc gtgtacggtg ggaggcctat ataagcagag ctcgtttagt gaaccgtcag
     2881 atcgcctgga gaattgcgta cggccagcat gcccctcaac gttagcttca ccaacaggaa
     2941 ctatgacctc gactacgact cggtgcagcc gtatttctac tgcgacgagg aggagaactt
     3001 ctaccagcag cagcagcaga gcgagctgca gcccccggcg cccagcgagg atatctggaa
     3061 gaaattcgag ctgctgccca ccccgcccct gtcccctagc cgccgctccg ggctctgctc
     3121 gccctcctac gttgcggtca cacccttctc ccttcgggga gacaacgacg gcggtggcgg
     3181 gagcttctcc acggccgacc agctggagat ggtgaccgag ctgctgggag gagacatggt
     3241 gaaccagagt ttcatctgcg acccggacga cgagaccttc atcaaaaaca tcatcatcca
     3301 ggactgtatg tggagcggct tctcggccgc cgccaagctc gtctcagaga agctggcctc
     3361 ctaccaggct gcgcgcaaag acagcggcag cccgaacccc gcccgcggcc acagcgtctg
     3421 ctccacctcc agcttgtacc tgcaggatct gagcgccgcc gcctcagagt gcatcgaccc
     3481 ctcggtggtc ttcccctacc ctctcaacga cagcagctcg cccaagtcct gcgcctcgca
     3541 agactccagc gccttctctc cgtcctcgga ttctctgctc tcctcgacgg agtcctcccc
     3601 gcagggcagc cccgagcccc tggtgctcca tgaggagaca ccgcccacca ccagcagcga
     3661 ctctgaggag gaacaagaag atgaggaaga aatcgatgtt gtttctgtgg aaaagaggca
     3721 ggctcctggc aaaaggtcag agtctggatc accttctgct ggaggccaca gcaaacctcc
     3781 tcacagccca ctggtcctca agaggtgcca cgtctccaca catcagcaca actacgcagc
     3841 gcctccctcc actcggaagg actatcctgc tgccaagagg gtcaagttgg acagtgtcag
     3901 agtcctgaga cagatcagca acaaccgaaa atgcaccagc cccaggtcct cggacaccga
     3961 ggagaatgtc aagaggcgaa cacacaacgt cttggagcgc cagaggagga acgagctaaa
     4021 acggagcttt tttgccctgc gtgaccagat cccggagttg gaaaacaatg aaaaggcccc
     4081 caaggtagtt atccttaaaa aagccacagc atacatcctg tccgtccaag cagaggagca
     4141 aaagctcatt tctgaagagg acttgttgcg gaaacgacga gaacagttga aacacaaact
     4201 tgaacagcta cggaactctt gtgcgtaaga attcgctagc gatctggcct ccgcgccggg
     4261 ttttggcgcc tcccgcgggc gcccccctcc tcacggcgag cgctgccacg tcagacgaag
     4321 ggcgcaggag cgtcctgatc cttccgcccg gacgctcagg acagcggccc gctgctcata
     4381 agactcggcc ttagaacccc agtatcagca gaaggacatt ttaggacggg acttgggtga
     4441 ctctagggca ctggttttct ttccagagag cggaacaggc gaggaaaagt agtcccttct
     4501 cggcgattct gcggagggat ctccgtgggg cggtgaacgc cgatgattat ataaggacgc
     4561 gccgggtgtg gcacagctag ttccgtcgca gccgggattt gggtcgcggt tcttgtttgt
     4621 ggatcgctgt gatcgtcact tggtgagtag cgggctgctg ggctggccgg ggctttcgtg
     4681 gccgccgggc cgctcggtgg gacggaagcg tgtggagaga ccgccaaggg ctgtagtctg
     4741 ggtccgcgag caaggttgcc ctgaactggg ggttgggggg agcgcagcaa aatggcggct
     4801 gttcccgagt cttgaatgga agacgcttgt gaggcgggct gtgaggtcgt tgaaacaagg
     4861 tggggggcat ggtgggcggc aagaacccaa ggtcttgagg ccttcgctaa tgcgggaaag
     4921 ctcttattcg ggtgagatgg gctggggcac catctgggga ccctgacgtg aagtttgtca
     4981 ctgactggag aactcggttt gtcgtctgtt gcgggggcgg cagttatgcg gtgccgttgg
     5041 gcagtgcacc cgtacctttg ggagcgcgcg ccctcgtcgt gtcgtgacgt cacccgttct
     5101 gttggcttat aatgcagggt ggggccacct gccggtaggt gtgcggtagg cttttctccg
     5161 tcgcaggacg cagggttcgg gcctagggta ggctctcctg aatcgacagg cgccggacct
     5221 ctggtgaggg gagggataag tgaggcgtca gtttctttgg tcggttttat gtacctatct
     5281 tcttaagtag ctgaagctcc ggttttgaac tatgcgctcg gggttggcga gtgtgttttg
     5341 tgaagttttt taggcacctt ttgaaatgta atcatttggg tcaatatgta attttcagtg
     5401 ttagactagt aaattgtccg ctaaattctg gccgtttttg gcttttttgt tagacgaaga
     5461 agcttgtcac catgtctaga ctggacaaga gcaaagtcat aaacggcgct ctggaattac
     5521 tcaatggagt cggtatcgaa ggcctgacga caaggaaact cgctcaaaag ctgggagttg
     5581 agcagcctac cctgtactgg cacgtgaaga acaagcgggc cctgctcgat gccctgccaa
     5641 tcgagatgct ggacaggcat catacccact tctgccccct ggaaggcgag tcatggcaag
     5701 actttctgcg gaacaacgcc aagtcattcc gctgtgctct cctctcacat cgcgacgggg
     5761 ctaaagtgca tctcggcacc cgcccaacag agaaacagta cgaaaccctg gaaaatcagc
     5821 tcgcgttcct gtgtcagcaa ggcttctccc tggagaacgc actgtacgct ctgtccgccg
     5881 tgggccactt tacactgggc tgcgtattgg aggaacagga gcatcaagta gcaaaagagg
     5941 aaagagagac acctaccacc gattctatgc ccccacttct gagacaagca attgagctgt
     6001 tcgaccggca gggagccgaa cctgccttcc ttttcggcct ggaactaatc atatgtggcc
     6061 tggagaaaca gctaaagtgc gaaagcggcg ggccggccga cgcccttgac gattttgact
     6121 tagacatgct cccagccgat gcccttgacg actttgacct tgatatgctg cctgctgacg
     6181 ctcttgacga ttttgacctt gacatgctcc ccgggtaact cgagggatca agcttatcga
     6241 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     6301 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     6361 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     6421 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     6481 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     6541 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     6601 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct
     6661 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     6721 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     6781 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg
     6841 tcgagacctg gaaaaacatg gagcaatcac aagtagcaac acagcagcta ccaatgctgc
     6901 ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt
     6961 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     7021 ggggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat
     7081 ctaccacaca caaggctact tccctgattg gcagaactac acaccagggc cagggatcag
     7141 atatccactg acctttggat ggtgctacaa gctagtacca gttgagcaag agaaggtaga
     7201 agaagccaat gaaggagaga acacccgctt gttacaccct gtgagcctgc atgggatgga
     7261 tgacccggag agagaagtat tagagtggag gtttgacagc cgcctagcat ttcatcacat
     7321 ggcccgagag ctgcatccgg actgtactgg gtctctctgg ttagaccaga tctgagcctg
     7381 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     7441 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     7501 cttttagtca gtgtggaaaa tctctagcag ggcccgttta aacccgctga tcagcctcga
     7561 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     7621 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     7681 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     7741 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
     7801 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
     7861 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     7921 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     7981 atcggggcat ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     8041 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     8101 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     8161 accctatctc ggtctattct tttgatttat aagggatttt ggggatttcg gcctattggt
     8221 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
     8281 gttagggtgt ggaaagtccc caggctcccc aggcaggcag aagtatgcaa agcatgcatc
     8341 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     8401 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     8461 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     8521 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
     8581 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
     8641 atcagcacgt gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa
     8701 ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga
     8761 cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcccggg acttcgtgga
     8821 ggacgacttc gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg cggtccagga
     8881 ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta
     8941 cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac gcctccgggc cggccatgac
     9001 cgagatcggc gagcagccgt gggggcggga gttcgccctg cgcgacccgg ccggcaactg
     9061 cgtgcacttc gtggccgagg agcaggactg acacgtgcta cgagatttcg attccaccgc
     9121 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     9181 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
     9241 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     9301 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
     9361 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     9421 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     9481 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     9541 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     9601 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     9661 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     9721 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     9781 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     9841 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     9901 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     9961 cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta
    10021 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
    10081 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
    10141 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
    10201 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
    10261 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
    10321 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
    10381 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
    10441 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
    10501 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
    10561 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
    10621 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
    10681 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
    10741 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
    10801 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
    10861 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
    10921 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
    10981 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
    11041 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
    11101 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
    11161 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
    11221 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
    11281 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    11341 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    11401 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
    11461 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
    11521 atttccccga aaagtgccac ctgacgtc
//
