LOCUS       Exported               11220 bp DNA     circular SYN 03-MAR-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11220)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 3, 2023 from SnapGene Viewer 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11220
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     misc_feature    832..929
                     /label=R
     misc_feature    930..1011
                     /label=U5
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1677..1910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2413..2530
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2572..2886
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     misc_feature    2909..3889
                     /label=BMI1
     promoter        3918..5127
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     CDS             5144..5890
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    5914..6502
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     misc_feature    6701..7020
                     /label=dU3
     misc_feature    7021..7118
                     /label=R
     misc_feature    7119..7200
                     /label=U5
     polyA_signal    7233..7457
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7503..7931
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        7945..8275
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8126..8261
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        8323..8370
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8389..8763
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8893..9014
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(9465..10053)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10224..11084)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11085..11189)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     1561 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1621 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1681 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1741 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1801 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1861 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1921 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1981 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     2041 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     2101 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     2161 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     2221 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     2281 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     2341 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctac aaatggcagt
     2401 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     2461 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
     2521 tcaaaatttt cgggtttatt acagggacag cagaaattca ctttgaattg cgagtttact
     2581 ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgatg
     2641 tcgagtttac tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat
     2701 agagaacgta tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttatcc
     2761 ctatcagtga tagagaacgt atgtcgagtt tactccctat cagtgataga gaacgtatgt
     2821 cgaggtaggc gtgtacggtg ggaggcctat ataagcagag ctcgtttagt gaaccgtcag
     2881 atcgcctgga gaattgcgta cggccagcat gcatcgaaca acgagaatca agatcactga
     2941 gctaaatccc cacctgatgt gtgtgctttg tggagggtac ttcattgatg ccacaaccat
     3001 aatagaatgt ctacattcct tctgtaaaac gtgtattgtt cgttacctgg agaccagcaa
     3061 gtattgtcct atttgtgatg tccaagttca caagaccaga ccactactga atataaggtc
     3121 agataaaact ctccaagata ttgtatacaa attagttcca gggcttttca aaaatgaaat
     3181 gaagagaaga agggattttt atgcagctca tccttctgct gatgctgcca atggctctaa
     3241 tgaagataga ggagaggttg cagatgaaga taagagaatt ataactgatg atgagataat
     3301 aagcttatcc attgaattct ttgaccagaa cagattggat cggaaagtaa acaaagacaa
     3361 agagaaatct aaggaggaag tgaatgataa aagatactta cgatgcccag cagcaatgac
     3421 tgtgatgcac ttaagaaagt ttctcagaag taaaatggac atacctaata ctttccagat
     3481 tgatgtcatg tatgaggagg aacctttaaa ggattattat acactaatgg atattgccta
     3541 catttatacc tggagaagga atggtccact tccattgaaa tacagagttc gacctacttg
     3601 taaaagaatg aagatcagtc accagagaga tggactgaca aatgctggag aactggaaag
     3661 tgactctggg agtgacaagg ccaacagccc agcaggaggt attccctcca cctcttcttg
     3721 tttgcctagc cccagtactc cagtgcagtc tcctcatcca cagtttcctc acatttccag
     3781 tactatgaat ggaaccagca acagccccag cggtaaccac caatcttctt ttgccaatag
     3841 acctcgaaaa tcatcagtaa atgggtcatc agcaacttct tctggttgaa ccggtctcga
     3901 gaattcgcta gcgatctggc ctccgcgccg ggttttggcg cctcccgcgg gcgcccccct
     3961 cctcacggcg agcgctgcca cgtcagacga agggcgcagg agcgtcctga tccttccgcc
     4021 cggacgctca ggacagcggc ccgctgctca taagactcgg ccttagaacc ccagtatcag
     4081 cagaaggaca ttttaggacg ggacttgggt gactctaggg cactggtttt ctttccagag
     4141 agcggaacag gcgaggaaaa gtagtccctt ctcggcgatt ctgcggaggg atctccgtgg
     4201 ggcggtgaac gccgatgatt atataaggac gcgccgggtg tggcacagct agttccgtcg
     4261 cagccgggat ttgggtcgcg gttcttgttt gtggatcgct gtgatcgtca cttggtgagt
     4321 agcgggctgc tgggctggcc ggggctttcg tggccgccgg gccgctcggt gggacggaag
     4381 cgtgtggaga gaccgccaag ggctgtagtc tgggtccgcg agcaaggttg ccctgaactg
     4441 ggggttgggg ggagcgcagc aaaatggcgg ctgttcccga gtcttgaatg gaagacgctt
     4501 gtgaggcggg ctgtgaggtc gttgaaacaa ggtggggggc atggtgggcg gcaagaaccc
     4561 aaggtcttga ggccttcgct aatgcgggaa agctcttatt cgggtgagat gggctggggc
     4621 accatctggg gaccctgacg tgaagtttgt cactgactgg agaactcggt ttgtcgtctg
     4681 ttgcgggggc ggcagttatg cggtgccgtt gggcagtgca cccgtacctt tgggagcgcg
     4741 cgccctcgtc gtgtcgtgac gtcacccgtt ctgttggctt ataatgcagg gtggggccac
     4801 ctgccggtag gtgtgcggta ggcttttctc cgtcgcagga cgcagggttc gggcctaggg
     4861 taggctctcc tgaatcgaca ggcgccggac ctctggtgag gggagggata agtgaggcgt
     4921 cagtttcttt ggtcggtttt atgtacctat cttcttaagt agctgaagct ccggttttga
     4981 actatgcgct cggggttggc gagtgtgttt tgtgaagttt tttaggcacc ttttgaaatg
     5041 taatcatttg ggtcaatatg taattttcag tgttagacta gtaaattgtc cgctaaattc
     5101 tggccgtttt tggctttttt gttagacgaa gaagcttgtc accatgtcta gactggacaa
     5161 gagcaaagtc ataaacggcg ctctggaatt actcaatgga gtcggtatcg aaggcctgac
     5221 gacaaggaaa ctcgctcaaa agctgggagt tgagcagcct accctgtact ggcacgtgaa
     5281 gaacaagcgg gccctgctcg atgccctgcc aatcgagatg ctggacaggc atcataccca
     5341 cttctgcccc ctggaaggcg agtcatggca agactttctg cggaacaacg ccaagtcatt
     5401 ccgctgtgct ctcctctcac atcgcgacgg ggctaaagtg catctcggca cccgcccaac
     5461 agagaaacag tacgaaaccc tggaaaatca gctcgcgttc ctgtgtcagc aaggcttctc
     5521 cctggagaac gcactgtacg ctctgtccgc cgtgggccac tttacactgg gctgcgtatt
     5581 ggaggaacag gagcatcaag tagcaaaaga ggaaagagag acacctacca ccgattctat
     5641 gcccccactt ctgagacaag caattgagct gttcgaccgg cagggagccg aacctgcctt
     5701 ccttttcggc ctggaactaa tcatatgtgg cctggagaaa cagctaaagt gcgaaagcgg
     5761 cgggccggcc gacgcccttg acgattttga cttagacatg ctcccagccg atgcccttga
     5821 cgactttgac cttgatatgc tgcctgctga cgctcttgac gattttgacc ttgacatgct
     5881 ccccgggtaa ctcgagggat caagcttatc gataatcaac ctctggatta caaaatttgt
     5941 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     6001 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     6061 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     6121 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     6181 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     6241 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     6301 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc
     6361 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     6421 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     6481 tccctttggg ccgcctcccc gcatcgatac cgtcgagacc tggaaaaaca tggagcaatc
     6541 acaagtagca acacagcagc taccaatgct gcttgtgcct ggctagaagc acaagaggag
     6601 gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca
     6661 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     6721 caacgaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat
     6781 tggcagaact acacaccagg gccagggatc agatatccac tgacctttgg atggtgctac
     6841 aagctagtac cagttgagca agagaaggta gaagaagcca atgaaggaga gaacacccgc
     6901 ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt attagagtgg
     6961 aggtttgaca gccgcctagc atttcatcac atggcccgag agctgcatcc ggactgtact
     7021 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     7081 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     7141 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     7201 agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     7261 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     7321 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     7381 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     7441 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
     7501 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     7561 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     7621 cacgttcgcc ggctttcccc gtcaagctct aaatcggggc atccctttag ggttccgatt
     7681 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     7741 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     7801 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     7861 ataagggatt ttggggattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     7921 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     7981 ccaggcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     8041 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     8101 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     8161 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
     8221 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     8281 cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg
     8341 gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagttg
     8401 accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc
     8461 gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac
     8521 gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc
     8581 tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg
     8641 aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg
     8701 gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga ggagcaggac
     8761 tgacacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     8821 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     8881 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     8941 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     9001 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca
     9061 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     9121 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     9181 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     9241 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     9301 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     9361 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     9421 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     9481 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     9541 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     9601 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa
     9661 tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     9721 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     9781 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     9841 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     9901 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     9961 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
    10021 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
    10081 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
    10141 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
    10201 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
    10261 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
    10321 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
    10381 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
    10441 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
    10501 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
    10561 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
    10621 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
    10681 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
    10741 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
    10801 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
    10861 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    10921 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    10981 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    11041 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    11101 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    11161 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
//
