LOCUS       pBR322-Pdp1_NTD-Cas9_v2       11589 bp ds-DNA     circular     04-NOV-2024
DEFINITION  .
ACCESSION   urn.local...1p-f9bzcd7
VERSION     urn.local...1p-f9bzcd7
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     RBS             complement(4..8)
                     /label="bla RBS"
     misc_feature    214..242
                     /label="Tet promoter"
     misc_feature    290..292
                     /label="TetR start codon"
     misc_feature    910..1110
                     /label="pvc17 effector - sepC"
     misc_feature    1111..1140
                     /label="2xGGSGG"
     misc_feature    1141..1284
                     /label="SV40 NLS"
     misc_feature    1144..1164
                     /label="NLS"
     misc_feature    1165..1260
                     /created_by="jkreitz"
                     /label="Artefact"
     misc_feature    1285..5388
                     /label="Cas9"
     misc_feature    5389..5433
                     /label="NLS"
     misc_feature    5434..5436
                     /label="pvc17 effector - sepC"
     misc_feature    5676..5918
                     /label="PAU_RS16570"
     misc_feature    6447..6698
                     /label="PAU_RS16565"
     misc_feature    6832..7128
                     /label="PAU_RS16560"
     misc_feature    7366..7572
                     /label="PAU_RS24015"
     RBS             9337..9341
                     /label="rop RBS"
     misc_feature    9347..9538
                     /label="rop CDS"
     rep_origin      complement(9966..10554)
                     /label="pMB1 origin of replication
                     (counter-clockwise)(RNAII -35 to RNA/DNA switch point)"
     misc_feature    complement(10725..11585)
                     /label="bla CDS"
     misc_feature    complement(11586..101)
                     /label="AmpR promoter"
ORIGIN      
        1 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
       61 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
      121 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
      181 acgaggccct ttcgtcttca agaattctca tgtttgacag cttatcatcg ataagcttta
      241 atgcggtagt ttatcacagt taaattgcta acgcagtcag gcaccgtgta tgaaatctaa
      301 caatgcgctc atcgtcatcc tcggcaccgt caccctggat gctgtaggca taggcttggt
      361 tatgccggta ctgccgggcc tcttgcggga tatcgtccat tccgacagca tcgccagtca
      421 ctatggcgtg ctgctagcgc tatatgcgtt gatgcaattt ctatgcgcac ccgttctcgg
      481 agcactgtcc gaccgctttg gccgccgccc agtcctgctc gcttcgctac ttggagccac
      541 tatcgactac gcgatcatgg cgaccacacc cgtcctgtgg atccattttt ttaattaaat
      601 aaaggaatta atattgattt gatagagcat gtaatcaata acttctatga aaatgttgaa
      661 aaacaataca cggaaacact taacttacaa ttaaattgtt aaaggagatg atgataagaa
      721 tggtcagtca ccagataaaa tgatctctgg tcaattttag gaaagctgaa aaaacctgac
      781 caaatgcgtc gtaaattcta tgcttagaaa gactcggtgc cagtgtaata cctaagatat
      841 ggtttttatt tccatatctg ttcgaaaact tagaaataac aagttcaaca ataaccaggg
      901 actttaaata tgcctagata tgctaattat cagataaacc ccaaacagaa tattaaaaat
      961 tcacatggga aatcttcatc gtcagatttt tctagtgggt acctttcatt ctcaaataat
     1021 tcgcttgatg acccttttat tcggcagcaa gttaagagag aatttatttg ggagggacat
     1081 atgaaggaga ttgaagaagc ttcaagatta ggtggctctg gtggtggcgg ttctggcggc
     1141 gcaccgaaaa agaaacgcaa agtttgggag ggacatatga aggagattga agaagcttca
     1201 agattaggtg gctctggtgg tggcggttct ggcggcgcac cgaaaaagaa acgcaaagtt
     1261 ggtattcatg gtgttccggc agcaatggat aagaaatact caataggctt agatatcggc
     1321 acaaatagcg tcggatgggc ggtgatcact gatgaatata aggttccgtc taaaaagttc
     1381 aaggttctgg gaaatacaga ccgccacagt atcaaaaaaa atcttatagg ggctctttta
     1441 tttgacagtg gagagacagc ggaagcgact cgtctcaaac ggacagctcg tagaaggtat
     1501 acacgtcgga agaatcgtat ttgttatcta caggagattt tttcaaatga gatggcgaaa
     1561 gtagatgata gtttctttca tcgacttgaa gagtcttttt tggtggaaga agacaagaag
     1621 catgaacgtc atcctatttt tggaaatata gtagatgaag ttgcttatca tgagaaatat
     1681 ccaactatct atcatctgcg aaaaaaattg gtagattcta ctgataaagc ggatttgcgc
     1741 ttaatctatt tggccttagc gcatatgatt aagtttcgtg gtcatttttt gattgaggga
     1801 gatttaaatc ctgataatag tgatgtggac aaactattta tccagttggt acaaacctac
     1861 aatcaattat ttgaagaaaa ccctattaac gcaagtggag tagatgctaa agcgattctt
     1921 tctgcacgat tgagtaaatc aagacgatta gaaaatctca ttgctcagct ccccggtgag
     1981 aagaaaaatg gcttatttgg gaatctcatt gctttgtcat tgggtttgac ccctaatttt
     2041 aaatcaaatt ttgatttggc agaagatgct aaattacagc tttcaaaaga tacttacgat
     2101 gatgatttag ataatttatt ggcgcaaatt ggagatcaat atgctgattt gtttttggca
     2161 gctaagaatt tatcagatgc tattttactt tcagatatcc taagagtaaa tactgaaata
     2221 actaaggctc ccctatcagc ttcaatgatt aaacgctacg atgaacatca tcaagacttg
     2281 actcttttaa aagctttagt tcgacaacaa cttccagaaa agtataaaga aatctttttt
     2341 gatcaatcaa aaaacggata tgcaggttat attgatgggg gagctagcca agaagaattt
     2401 tataaattta tcaaaccaat tttagaaaaa atggatggta ctgaggaatt attggtgaaa
     2461 ctaaatcgtg aagatttgct gcgcaagcaa cggacctttg acaacggctc tattccccat
     2521 caaattcact tgggtgagct gcatgctatt ttgagaagac aagaagactt ttatccattt
     2581 ttaaaagaca atcgtgagaa gattgaaaaa atcttgactt ttcgaattcc ttattatgtt
     2641 ggtccattgg cgcgtggcaa tagtcgtttt gcatggatga ctcggaagtc tgaagaaaca
     2701 attaccccat ggaattttga agaagttgtc gataaaggtg cttcagctca atcatttatt
     2761 gaacgcatga caaactttga taaaaatctt ccaaatgaaa aagtactacc aaaacatagt
     2821 ttgctttatg agtattttac ggtttataac gaattgacaa aggtcaaata tgttactgaa
     2881 ggaatgcgaa aaccagcatt tctttcaggt gaacagaaga aagccattgt tgatttactc
     2941 ttcaaaacaa atcgaaaagt aaccgttaag caattaaaag aagattattt caaaaaaata
     3001 gaatgttttg atagtgttga aatttcagga gttgaagata gatttaatgc ttcattaggt
     3061 acctaccatg atttgctaaa aattattaaa gataaagatt ttttggataa tgaagaaaat
     3121 gaagatatct tagaggatat tgttttaaca ttgaccttat ttgaagatag ggagatgatt
     3181 gaggaaagac ttaaaacata tgctcacctc tttgatgata aggtgatgaa acagcttaaa
     3241 cgtcgccgtt atactggttg gggacgtttg tctcgaaaat tgattaatgg tattagggat
     3301 aagcaatctg gcaaaacaat attagatttt ttgaaatcag atggttttgc caatcgcaat
     3361 tttatgcagc tgatccatga tgatagtttg acatttaaag aagacattca aaaagcacaa
     3421 gtgtctggac aaggcgatag tttacatgaa catattgcaa atttagctgg tagccctgct
     3481 attaaaaaag gtattttaca gactgtaaaa gttgttgatg aattggtcaa agtaatgggg
     3541 cggcataagc cagaaaatat cgttattgaa atggcacgtg aaaatcagac aactcaaaag
     3601 ggccagaaaa attcgcgaga gcgtatgaaa cgaatcgaag aaggtatcaa agaattagga
     3661 agtcagattc ttaaagagca tcctgttgaa aatactcaat tgcaaaatga aaagctctat
     3721 ctctattatc tccaaaatgg aagagacatg tatgtggacc aagaattaga tattaatcgt
     3781 ttaagtgatt atgatgtcga tcacattgtt ccacaaagtt tccttaaaga cgattcaata
     3841 gacaataagg tcttaacgcg ttctgataaa aatcgtggta aatcggataa cgttccaagt
     3901 gaagaagtag tcaaaaagat gaaaaactat tggagacaac ttctaaacgc caagttaatc
     3961 actcaacgta agtttgataa tttaacgaaa gctgaacgtg gaggtttgag tgaacttgat
     4021 aaagctggtt ttatcaaacg ccaattggtt gaaactcgcc aaatcactaa gcatgtggca
     4081 caaattttgg atagtcgcat gaatactaaa tacgatgaaa atgataaact tattcgagag
     4141 gttaaagtga ttaccttaaa atctaaatta gtttctgact tccgaaaaga tttccaattc
     4201 tataaagtac gtgagattaa caattaccat catgcccatg atgcgtatct aaatgccgtc
     4261 gttggaactg ctttgattaa gaaatatcca aaacttgaat cggagtttgt ctatggtgat
     4321 tataaagttt atgatgttcg taaaatgatt gctaagtctg agcaagaaat aggcaaagca
     4381 accgcaaaat atttctttta ctctaatatc atgaacttct tcaaaacaga aattacactt
     4441 gcaaatggag agattcgcaa acgccctcta atcgaaacta atggggaaac tggagaaatt
     4501 gtctgggata aagggcgaga ttttgccaca gtgcgcaaag tattgtccat gccccaagtc
     4561 aatattgtca agaaaacaga agtacagaca ggcggattct ccaaggagtc aattttacca
     4621 aaaagaaatt cggacaagct tattgctcgt aaaaaagact gggatccaaa aaaatatggt
     4681 ggttttgata gtccaacggt agcttattca gtcctagtgg ttgctaaggt ggaaaaaggg
     4741 aaatcgaaga agttaaaatc cgttaaagag ttactaggga tcacaattat ggaaagaagt
     4801 tcctttgaaa aaaatccgat tgacttttta gaagctaaag gatataagga agttaaaaaa
     4861 gacttaatca ttaaactacc taaatatagt ctttttgagt tagaaaacgg tcgtaaacgg
     4921 atgctggcta gtgccggaga attacaaaaa ggaaatgagc tggctctgcc aagcaaatat
     4981 gtgaattttt tatatttagc tagtcattat gaaaagttga agggtagtcc agaagataac
     5041 gaacaaaaac aattgtttgt ggagcagcat aagcattatt tagatgagat tattgagcaa
     5101 atcagtgaat tttctaagcg tgttatttta gcagatgcca atttagataa agttcttagt
     5161 gcatataaca aacatagaga caaaccaata cgtgaacaag cagaaaatat tattcattta
     5221 tttacgttga cgaatcttgg agctcccgct gcttttaaat attttgatac aacaattgat
     5281 cgtaaacgat atacgtctac aaaagaagtt ttagatgcca ctcttatcca tcaatccatc
     5341 actggtcttt atgaaacacg cattgatttg agtcagctag gaggtgacaa acgtccggca
     5401 gcaaccaaaa aggcaggtca ggccaaaaag aaatgatatg gattttcatg tcatcaggag
     5461 aatattcaaa attctgtgtc acacaaaata ttttacagcg taatcacacc attaagaggg
     5521 gcatcaaaat aaatggttaa ctgggacaat gttgaatttc atttttataa ggcattgtcc
     5581 gtattatcca ccatccagat caacggattc agatggtgta aagaaagcat atagctggta
     5641 ggtaaaatta agaagtgtag ttttataaag tattaatgga gaacgatgaa tttaaagtaa
     5701 atggaggaag gttagtttca ctatcaaatt acaggaccaa aaatatggag tattccatct
     5761 tctttggtga attaagtctt gaagacttca acaaatattg cctggaacta tgggcctgtg
     5821 aatggtattg gagtatcatt tataacgact atttcgatag gaaatttaaa gtcttgttga
     5881 aaatgactaa tcgtctaaaa gtatgtctgg agaaataggt ttaatctcta tatttaacgg
     5941 ttttattttc atctatttac taatggtaaa tttttttact ctttgaatat attggtgagt
     6001 ttgttatccc cattagttac cagggtatta ttaatgagtg aaattcaatt ttattgatat
     6061 taaagtcaaa tttacaaatg atgactttat tattttttat ttattaattt taaattattt
     6121 tttgttaatc tttactataa atgaattttt tgcttgcatg gacttgattt ttttatatat
     6181 aacattgaca aatattaatt catatatatg attgttatag tgagttattt aattgattcg
     6241 actaacaatt tcattgtaat ttttcaagat agcagtcaat tgctataaca agcatctttc
     6301 tcgtcaaagg aaaatgtgta tggcctataa gcagcaactt gaaagatgaa ggatataatg
     6361 atgattaact taacggagag tagatattag aagtagtaga aggacaattt aagactttat
     6421 acccattaaa acaaaggggt aaaataatga tgtttgatga aaatttagaa tgtgttaatc
     6481 acgaaattat caatgaaaaa ttagatacga aaattcacct tgctaatcct tatgcctttt
     6541 gggggggaag ggtaaatgag aatataaatg ggttaattaa acaacattct ttaagaggaa
     6601 ctgactttaa taaaattttt aaccggaaga taaatttttt tgtaaatgga ataaataatt
     6661 gcccccgaaa aactcggaat ataaaacccc ctaattaatt atttaaagga ataagagttt
     6721 atttgctcgt agagtaatta tgttccatta atatgctaaa ttgctcagta aagttattaa
     6781 ctttttttca acgccagtga ataagtctgt tttattaaat aaggagttca ggtgtttatt
     6841 tcaaaagaat tatcaagctt tattgctgta gctaaaaata aatcaataaa taaagcaaca
     6901 acggaacttt ttattacaca gtcaccgatt tgccgaagct taaaaaaact ggaattttca
     6961 ttaggtgtaa aactctttgt aagaaaaagc cagggattgg ctttaacaga agaaggagaa
     7021 tgcttatata ataaagtttt gtcaatttgt gaacggcttc aagaaataga aggtgactac
     7081 aagaatgaaa aatcaacata caatgatcta tatcaaaaaa atatatgagg ttctaatata
     7141 ataagcggtt ctagataact aaagtcatct attaccaata tctgtatact cagtgaaagt
     7201 tttgtcttct aggtaagttt tcataatagt gacatacctt attttttcat ctgggtgtct
     7261 ctcatcctga agaatgggtg atatagttga aataactcag ttcgttttgt ggtttacgat
     7321 ttttggcgga tcacaaaaat agggctgagt cccttcaagt gagttttgaa atatacaatt
     7381 atacccttca tcctttaagt tgttgctttg ttggctgttt tcattcacct cagtcacata
     7441 gttatctatg ctcctgagga ttcgttcact tgccgtcgtg ctgcaactcg aaatctattg
     7501 ggtatatatg taattttcat attctgtttc attttaacgg tattatttaa atatttatat
     7561 ttagaacatt aggttattag aatagataat tttaggtaaa tgtaattaat tcaaccatca
     7621 cggagagttt atcattttgc ccaatgaggg caggaggtac ggattttcat tagcacttat
     7681 cgaagcggat atatgtttgt gactacattg tcagaaaatt tcattcatca acttgaatta
     7741 gaacagtgtc catataactt atttagaata aaggaaggaa attaatctta gatacaatga
     7801 taagggaaat aaaatagaaa gtagagttaa ttgtattaat aaggtgctgg agctttactt
     7861 acaaaatatc aatgattttt ggtttcgtat aagtaattaa tttagagtaa tgaaaaatta
     7921 tttgtgggtt aaattttatt tattgaaaat tcattggttt attttatgaa ataggaaggt
     7981 aatagcggta ttattcggtt gaaataaccg tatagataaa tagtaggatt tagataaatt
     8041 atttcatata tgacaatttt tactattata tggggtttgt aagtcgaccg atgcccttga
     8101 gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac
     8161 ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg ctctgggtca
     8221 ttttcggcga ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg cttgcggtat
     8281 tcggaatctt gcacgccctc gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg
     8341 gcgagaagca ggccattatc gccggcatgg cggccgacgc gctgggctac gtcttgctgg
     8401 cgttcgcgac gcgaggctgg atggccttcc ccattatgat tcttctcgct tccggcggca
     8461 tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt agatgacgac catcagggac
     8521 agcttcaagg atcgctcgcg gctcttacca gcctaacttc gatcattgga ccgctgatcg
     8581 tcacggcgat ttatgccgcc tcggcgagca catggaacgg gttggcatgg attgtaggcg
     8641 ccgccctata ccttgtctgc ctccccgcgt tgcgtcgcgg tgcatggagc cgggccacct
     8701 cgacctgaat ggaagccggc ggcacctcgc taacggattc accactccaa gaattggagc
     8761 caatcaattc ttgcggagaa ctgtgaatgc gcaaaccaac ccttggcaga acatatccat
     8821 cgcgtccgcc atctccagca gccgcacgcg gcgcatctcg ggcagcgttg ggtcctggcc
     8881 acgggtgcgc atgatcgtgc tcctgtcgtt gaggacccgg ctaggctggc ggggttgcct
     8941 tactggttag cagaatgaat caccgatacg cgagcgaacg tgaagcgact gctgctgcaa
     9001 aacgtctgcg acctgagcaa caacatgaat ggtcttcggt ttccgtgttt cgtaaagtct
     9061 ggaaacgcgg aagtcagcgc cctgcaccat tatgttccgg atctgcatcg caggatgctg
     9121 ctggctaccc tgtggaacac ctacatctgt attaacgaag cgctggcatt gaccctgagt
     9181 gatttttctc tggtcccgcc gcatccatac cgccagttgt ttaccctcac aacgttccag
     9241 taaccgggca tgttcatcat cagtaacccg tatcgtgagc atcctctctc gtttcatcgg
     9301 tatcattacc cccatgaaca gaaatccccc ttacacggag gcatcagtga ccaaacagga
     9361 aaaaaccgcc cttaacatgg cccgctttat cagaagccag acattaacgc ttctggagaa
     9421 actcaacgag ctggacgcgg atgaacaggc agacatctgt gaatcgcttc acgaccacgc
     9481 tgatgagctt taccgcagct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     9541 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     9601 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg
     9661 tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga
     9721 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
     9781 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     9841 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     9901 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     9961 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
    10021 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
    10081 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
    10141 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
    10201 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
    10261 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
    10321 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
    10381 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
    10441 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
    10501 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
    10561 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
    10621 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
    10681 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
    10741 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
    10801 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
    10861 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
    10921 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
    10981 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
    11041 gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
    11101 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
    11161 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
    11221 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
    11281 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
    11341 acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
    11401 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
    11461 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
    11521 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
    11581 ctcatactc
//
