LOCUS       TwinStrep-SUMO-Pdp1_updated        7094 bp ds-DNA     circular     27-MAR-2023
DEFINITION  .
ACCESSION   urn.local...kmi-exry1bj
VERSION     urn.local...kmi-exry1bj
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    complement(324..1331)
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /modified_by="jkreitz"
                     /label="pvc17 payload - Pdp1"
     misc_feature    complement(1131..1331)
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /label="NTD"
     misc_feature    complement(1337..1628)
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /label="Sumo"
     misc_feature    complement(1629..1781)
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /label="6x His tag/ Twin Strep"
     misc_binding    complement(1827..1849)
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /label="LacO"
     rep_origin      complement(5249..5931)
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /label="ColE1 origin"
     CDS             complement(6029..6688)
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /label="AmpR"
ORIGIN      
        1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
       61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
      121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
      181 gcgggatatc cggatatagt tcctcctttc agcaaaaaac ccctcaagac ccgtttagag
      241 gccccaaggg gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt
      301 cgggcctcga gtgcggccgc tcatcttttc cggctaaatc tggccatgcc cggtctttgt
      361 ggattagaaa cgaatccagg attttcagca cccggcttcc agctcacttt catttttgca
      421 ccgatactat tataaaactg atttagttca tcaatgttgt tggcaattgt ccattttcct
      481 ttgctaacaa tagcgacagg aaattcaccg ggttcagcaa cagccttgac aatccccccg
      541 tgttttttta tttcctcttt ttctttatcc atagcaaaag ccgggtagct tacctgggct
      601 ccatgcctga taacattatg ctctatatct ccaaatggcc ggtttgaatc agatcgagca
      661 accaatcggt taatgcgatc tataattttc ctttcctctg atgagttgga tggtactgaa
      721 tgaggttgac ctgtgaaact aatcatatca tgcatatcat agtcaccagt aaaaggtagt
      781 gcagcccagt tctcttttgc tttcaaggct gagagactcg cttccagatt gtttaagtct
      841 ataggataaa tttttccatt gacctgttca tctgataaac cgtgaccact actcatgtaa
      901 atacccaaaa ggcggccagt ttttttatcc caatggccta catagccttc aatacccgct
      961 tcctgtactt tctttattac atctgaagct tcgtctttgg gataagcttt gttaattgag
     1021 ccgggtttaa tggttttttc caaaatatca tgccccttgg ctgccgcacc tttgcccaaa
     1081 gctctgagtg ttggcccgcc cgcagcccga aaactgacag caaaattacc taatcttgaa
     1141 gcttcttcaa tctccttcat atgtccctcc caaataaatt ctctcttaac ttgctgccga
     1201 ataaaagggt catcaagcga attatttgag aatgaaaggt acccactaga aaaatctgac
     1261 gatgaagatt tcccatgtga atttttaata ttctgtttgg ggtttatctg ataattagca
     1321 tatctaggca tggatccacc aatctgttct ctgtgagcct caataatatc gttatcctcc
     1381 atgtccaaat cttcaggggt ctgatcagct tggattctaa taccgtcgta caagaatctt
     1441 aaggagtcca tttccttacc ctgtctttta gcgaacgctt ccatcagcct tcttaaagga
     1501 gtggtctttt tgatcttgaa gaagatctct gaagatccat cggacacctt taaattgatg
     1561 tgagtctcag gcttgacttc tggcttgacc tctggcttag cttcttgatt gacttctgag
     1621 tccgacattt tctcaaactg agggtgtgac catgcactac cacctgaacc accaccgcta
     1681 ccaccacctt tttcaaactg cggatggctc cagctagcca tatggctgcc gcgcggcacc
     1741 aggccgctgc tgtgatgatg atgatgatgg ctgctgccca tggtatatct ccttcttaaa
     1801 gttaaacaaa attatttcta gaggggaatt gttatccgct cacaattccc ctatagtgag
     1861 tcgtattaat ttcgcgggat cgagatctcg atcctctacg ccggacgcat cgtggccggc
     1921 atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa
     1981 gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc
     2041 cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg
     2101 gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga
     2161 gagcgtcgag atcccggaca ccatcgaatg gcgcaaaacc tttcgcggta tggcatgata
     2221 gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa ccagtaacgt tatacgatgt
     2281 cgcagagtat gccggtgtct cttatcagac cgtttcccgc gtggtgaacc aggccagcca
     2341 cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg gcggagctga attacattcc
     2401 caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg ctgattggcg ttgccacctc
     2461 cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca
     2521 actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc
     2581 ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg atcattaact atccgctgga
     2641 tgaccaggat gccattgctg tggaagctgc ctgcactaat gttccggcgt tatttcttga
     2701 tgtctctgac cagacaccca tcaacagtat tattttctcc catgaagacg gtacgcgact
     2761 gggcgtggag catctggtcg cattgggtca ccagcaaatc gcgctgttag cgggcccatt
     2821 aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat aaatatctca ctcgcaatca
     2881 aattcagccg atagcggaac gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac
     2941 catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca acgatcagat
     3001 ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg cggatatctc
     3061 ggtagtggga tacgacgata ccgaagacag ctcatgttat atcccgccgt taaccaccat
     3121 caaacaggat tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca
     3181 gggccaggcg gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac
     3241 cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     3301 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtaagtt
     3361 agctcactca ttaggcaccg ggatctcgac cgatgccctt gagagccttc aacccagtca
     3421 gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta
     3481 tcatgcaact cgtaggacag gtgccggcag cgctctgggt cattttcggc gaggaccgct
     3541 ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt attcggaatc ttgcacgccc
     3601 tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt cggcgagaag caggccatta
     3661 tcgccggcat ggcggccgac gcgctgggct acgtcttgct ggcgttcgcg acgcgaggct
     3721 ggatggcctt ccccattatg attcttctcg cttccggcgg catcgggatg cccgcgttgc
     3781 aggccatgct gtccaggcag gtagatgacg accatcaggg acagcttcaa ggatcgctcg
     3841 cggctcttac cagcctaact tcgatcattg gaccgctgat cgtcacggcg atttatgccg
     3901 cctcggcgag cacatggaac gggttggcat ggattgtagg cgccgcccta taccttgtct
     3961 gcctccccgc gttgcgtcgc ggtgcatgga gccgggccac ctcgacctga atggaagccg
     4021 gcggcacctc gctaacggat tcaccactcc aagaattgga gccaatcaat tcttgcggag
     4081 aactgtgaat gcgcaaacca acccttggca gaacatatcc atcgcgtccg ccatctccag
     4141 cagccgcacg cggcgcatct cgggcagcgt tgggtcctgg ccacgggtgc gcatgatcgt
     4201 gctcctgtcg ttgaggaccc ggctaggctg gcggggttgc cttactggtt agcagaatga
     4261 atcaccgata cgcgagcgaa cgtgaagcga ctgctgctgc aaaacgtctg cgacctgagc
     4321 aacaacatga atggtcttcg gtttccgtgt ttcgtaaagt ctggaaacgc ggaagtcagc
     4381 gccctgcacc attatgttcc ggatctgcat cgcaggatgc tgctggctac cctgtggaac
     4441 acctacatct gtattaacga agcgctggca ttgaccctga gtgatttttc tctggtcccg
     4501 ccgcatccat accgccagtt gtttaccctc acaacgttcc agtaaccggg catgttcatc
     4561 atcagtaacc cgtatcgtga gcatcctctc tcgtttcatc ggtatcatta cccccatgaa
     4621 cagaaatccc ccttacacgg aggcatcagt gaccaaacag gaaaaaaccg cccttaacat
     4681 ggcccgcttt atcagaagcc agacattaac gcttctggag aaactcaacg agctggacgc
     4741 ggatgaacag gcagacatct gtgaatcgct tcacgaccac gctgatgagc tttaccgcag
     4801 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     4861 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     4921 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta
     4981 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca ttgcggtgtg
     5041 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc
     5101 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     5161 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     5221 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     5281 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     5341 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     5401 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     5461 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     5521 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     5581 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     5641 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     5701 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     5761 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     5821 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     5881 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     5941 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     6001 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     6061 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     6121 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     6181 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     6241 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     6301 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac
     6361 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     6421 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     6481 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     6541 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     6601 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc
     6661 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     6721 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     6781 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     6841 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     6901 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     6961 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     7021 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     7081 ttcgtcttca agaa
//
