LOCUS       Exported                7319 bp ds-DNA     circular SYN 09-FEB-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7319)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Feb 9, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     source          1..7319
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     polyA_signal    778..825
                     /label=HSV TK poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=98.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     rep_origin      1154..1742
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     enhancer        1917..2220
                     /label=CMV enhancer
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=enhancer"
     promoter        2221..2424
                     /label=CMV promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    2447..2469
                     /label=MCS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=28.4"
                     /note="/other=misc_feature"
     CDS             2475..5063
                     /codon_start=1
                     /label=hDyn1 D406A
                     /label=DNM1
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=99.9"
                     /note="/other=CDS"
                     /translation="MGNRGMEDLIPLVNRLQDAFSAIGQNADLDLPQIAVVGGQSAGKS
                     SVLENFVGRDFLPRGSGIVTRRPLVLQLVNATTEYAEFLHCKGKKFTDFEEVRLEIEAE
                     TDRVTGTNKGISPVPINLRVYSPHVLNLTLVDLPGMTKVPVGDQPPDIEFQIRDMLMQF
                     VTKENCLILAVSPANSDLANSDALKVAKEVDPQGQRTIGVITKLDLMDEGTDARDVLEN
                     KLLPLRRGYIGVVNRSQKDIDGKKDITAALAAERKFFLSHPSYRHLADRMGTPYLQKVL
                     NQQLTNHIRDTLPGLRNKLQSQLLSIEKEVEEYKNFRPDDPARKTKALLQMVQQFAVDF
                     EKRIEGSGDQIDTYELSGGARINRIFHERFPFELVKMEFDEKELRREISYAIKNIHGIR
                     TGLFTPAMAFETIVKKQVKKIREPCLKCVDMVISELISTVRQCTKKLQQYPRLREEMER
                     IVTTHIREREGRTKEQVMLLIDIELAYMNTNHEDFIGFANAQQRSNQMNKKKTSGNQDE
                     ILVIRKGWLTINNIGIMKGGSKEYWFVLTAENLSWYKDDEEKEKKYMLSVDNLKLRDVE
                     KGFMSSKHIFALFNTEQRNVYKDYRQLELACETQEEVDSWKASFLRAGVYPERVGDKEK
                     ASETEENGSDSFMHSMDPQLERQVETIRNLVDSYMAIVNKTVRDLMPKTIMHLMINNTK
                     EFIFSELLANLYSCGDQNTLMEESAEQAQRRDEMLRMYHALKEALSIIGNINTTTVSTP
                     MPPPVDDSWLQVQSVPAGRRSPTSSPTPQRRAPAVPPARPGSRGPAPGPPPAGSALGGA
                     PPVPSRPGASPDPFGPPPQVPSRPNRAPPGVPSRSGQASPSRPESPRPPFD"
     CDS             5121..5840
                     /codon_start=1
                     /label=EGFP
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    5963..6084
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     rep_origin      complement(6118..6546)
                     /direction=LEFT
                     /label=f1 ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     promoter        6573..6677
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     promoter        6679..7036
                     /label=SV40 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             join(7071..7319,1..546)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
ORIGIN
        1 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
       61 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
      121 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
      181 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
      241 gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat
      301 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
      361 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
      421 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
      481 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
      541 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
      601 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
      661 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccta
      721 gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg
      781 caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc
      841 ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc
      901 gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag
      961 ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata ctttagattg
     1021 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     1081 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     1141 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     1201 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     1261 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     1321 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     1381 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     1441 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     1501 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     1561 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     1621 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     1681 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     1741 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1801 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc atgcattagt
     1861 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
     1921 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     1981 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     2041 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     2101 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     2161 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     2221 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
     2281 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
     2341 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
     2401 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gcgctaccgg
     2461 actcagatcc tgccatgggc aaccgcggca tggaagatct catcccgctg gtcaaccggc
     2521 tgcaagacgc cttctctgcc atcggccaga acgcggacct cgacctgccg cagatcgctg
     2581 tggtgggcgg ccagagcgcc ggcaagagct cggtgctcga gaatttcgta ggcagggact
     2641 tcttgcctcg aggatctggc attgttaccc gacgtcccct ggtcttgcag ctggtcaatg
     2701 caaccacaga atatgccgag ttcctgcact gcaagggaaa gaaattcacc gacttcgagg
     2761 aggtgcgcct tgagatcgag gccgagaccg acagggtcac cggcaccaac aagggcatct
     2821 cgccggtgcc tatcaacctc cgcgtctact cgccgcacgt gctgaacctg accctggtgg
     2881 acctgcccgg aatgaccaag gtcccggtgg gggaccaacc tcccgacatc gagttccaga
     2941 tccgagacat gcttatgcag tttgtcacca aggagaactg cctcatcctg gccgtgtccc
     3001 ccgccaactc tgacctggcc aattctgacg ccctcaaggt cgccaaggag gtggaccccc
     3061 agggccagcg caccatcggg gtcatcacca agctggacct gatggacgag ggcacagatg
     3121 cccgtgatgt gctggagaac aagctgctcc ccctgcgcag aggctacatt ggagtggtga
     3181 accggagcca gaaggacatt gatggcaaga aggacattac cgccgccttg gctgctgaac
     3241 gaaagttctt cctctcccat ccatcttatc gccacttggc tgaccgtatg ggcacgccct
     3301 acctgcagaa ggtcctcaat cagcaactga cgaaccacat ccgggacaca ctgccggggc
     3361 tgcggaacaa gctgcagagc cagctactgt ccattgagaa ggaggtggag gaatacaaga
     3421 acttccgccc tgatgaccca gctcgcaaga ccaaggccct gctgcagatg gtccagcagt
     3481 ttgccgtaga ctttgagaag cgcattgagg gctcaggaga tcagatcgac acctacgaac
     3541 tgtcaggggg agcccgcatt aaccgaatct tccacgagcg cttccctttc gagctggtca
     3601 agatggagtt tgatgagaag gaactccgaa gggagatcag ctatgctatc aagaatatcc
     3661 atggcattag aacggggctg tttaccccag ccatggcctt tgagaccatt gtgaaaaagc
     3721 aggtgaagaa gatccgagaa ccgtgtctca agtgtgtgga catggttatc tcggagctaa
     3781 tcagcaccgt tagacagtgc accaagaagc tccagcagta cccgcggcta cgggaggaga
     3841 tggagcgcat cgtgaccacc cacatccggg agcgcgaggg ccgcactaag gagcaggtca
     3901 tgcttctcat cgatatcgag ctggcttaca tgaacaccaa ccatgaggac ttcataggct
     3961 ttgccaatgc tcagcagagg agcaaccaga tgaacaagaa gaagacttca gggaaccagg
     4021 atgagattct ggtcatccgc aagggctggc tgactatcaa taatattggc atcatgaaag
     4081 ggggctccaa ggagtactgg tttgtgctga ctgctgagaa tctgtcctgg tacaaggatg
     4141 atgaggagaa agagaagaaa tacatgctgt ctgtggacaa cctcaagctg cgggacgtgg
     4201 agaagggctt tatgtcgagc aagcatatct ttgccctctt taacacggag cagaggaatg
     4261 tctacaagga ttatcggcag ctggagctag cctgtgagac acaggaggag gtggacagct
     4321 ggaaggcctc cttcctgagg gctggcgtgt accctgagcg tgttggggac aaagagaaag
     4381 ccagcgagac cgaggagaat ggctccgaca gcttcatgca ttccatggac ccacagctgg
     4441 aacggcaagt ggagaccatc cggaatcttg tggactcata catggccatt gtcaacaaga
     4501 ccgtgaggga cctcatgccc aagaccatca tgcacctcat gattaacaat accaaggagt
     4561 tcatcttctc ggagctgctg gccaacctgt actcgtgtgg ggaccagaac acgctgatgg
     4621 aggagtcggc ggagcaggca cagcggcgcg acgagatgct gcgcatgtac cacgcactga
     4681 aggaggcgct cagcatcatc ggcaacatca acacgaccac cgtcagcacg cccatgcccc
     4741 cgcccgtgga cgactcctgg ctgcaggtgc agagcgtacc ggccggacgc aggtcgccca
     4801 cgtccagccc cacgccgcag cgccgagccc ccgccgtgcc cccagcccgg cccgggtcgc
     4861 ggggccctgc tcctgggcct ccgcctgctg ggtccgccct ggggggggcg ccccccgtgc
     4921 cctccaggcc gggggcttcc cctgaccctt tcggccctcc ccctcaggtg ccctcgcgcc
     4981 ccaaccgcgc cccgcccggg gtccccagcc gatcgggtca ggcaagtcca tcccgtcctg
     5041 agagccccag gccccccttc gacaagcttc gaattctgca gtcgacggta ccgcgggccc
     5101 gggatccacc ggtcgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc
     5161 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     5221 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     5281 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc
     5341 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     5401 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     5461 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     5521 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
     5581 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
     5641 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     5701 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
     5761 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     5821 tggacgagct gtacaagtaa agcggccgcg actctagatc ataatcagcc ataccacatt
     5881 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
     5941 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
     6001 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     6061 gtccaaactc atcaatgtat cttaaggcgt aaattgtaag cgttaatatt ttgttaaaat
     6121 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     6181 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     6241 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     6301 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     6361 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     6421 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     6481 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     6541 gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     6601 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     6661 attgaaaaag gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg
     6721 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     6781 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     6841 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     6901 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     6961 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     7021 cctaggcttt tgcaaagatc gatcaagaga caggatgagg atcgtttcgc atgattgaac
     7081 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     7141 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     7201 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg
     7261 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgtt
//
