LOCUS       pTET2-lucCP-3MS2        7394 bp DNA     circular SYN 08-SEP-2009
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pcTET2-BwtB2-3MS2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7394)
  AUTHORS   Suzanne Lee
  TITLE     Direct Submission
  JOURNAL   Exported Feb 9, 2023 from SnapGene 6.2.1
            https://www.snapgene.com
COMMENT     ORIGDB|GenBank
FEATURES             Location/Qualifiers
     source          1..7394
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        234..685
                     /label=pTET2
                     /note="/vntifkey=29"
     misc_feature    304..1249
                     /label=sequence confirmation, 20110729 Firefly luc R
                     /label=sequence\confirmation,\20110729\Firefly\luc\R
                     /label=nonstandard type:  insertion_seq
                     /note="/vntifkey=14"
     misc_feature    633..654
                     /label=pTet sequencing primer
                     /label=pTet\sequencing\primer
                     /note="/vntifkey=21"
     misc_feature    697..1538
                     /label=sequencing confirmation, p-Tet-primer 20110615
                     /label=sequencing\confirmation,\p-Tet-primer\20110615
                     /label=nonstandard type:  insertion_seq
                     /note="/vntifkey=14"
     misc_feature    699..2347
                     /label=hluc+
                     /note="/vntifkey=21"
     misc_feature    1195..1214
                     /label=Firefly luc F primer
                     /label=Firefly\luc\F\primer
                     /note="/vntifkey=21"
     misc_feature    1248..2110
                     /label=sequence confirmation, 20110729 Firefly luc F
                     /label=sequence\confirmation,\20110729\Firefly\luc\F
                     /label=nonstandard type:  insertion_seq
                     /note="/vntifkey=14"
     misc_feature    1284..1303
                     /label=Firefly luc R
                     /label=Firefly\luc\R
                     /note="/vntifkey=21"
     misc_feature    1528..2357
                     /label=align, 20130718 pTet-FlucCP3MS1R
                     /label=align,\20130718\pTet-FlucCP3MS1R
                     /label=nonstandard type:  insertion_seq
                     /note="/vntifkey=14"
     misc_feature    2113..2920
                     /label=sequence confirmation, 20110608 SP6, Tet-2
                     /label=sequence\confirmation,\20110608\SP6,\Tet-2
                     /label=nonstandard type:  insertion_seq
                     /note="/vntifkey=14"
     misc_feature    2322..2341
                     /label=Firefly XmnI F
                     /label=Firefly\XmnI\F
                     /note="/vntifkey=21"
     misc_feature    2349..2408
                     /label=CP1
                     /note="/vntifkey=21"
     misc_feature    2375..3274
                     /label=align, 20130718 pTetFlcuCP3MS21_FireflyXcmIF
                     /label=align,\20130718\pTetFlcuCP3MS21_FireflyXcmIF
                     /label=nonstandard type:  insertion_seq
                     /note="/vntifkey=14"
     misc_feature    2380..2400
                     /label=CP1 XmnI R
                     /label=CP1\XmnI\R
                     /note="/vntifkey=21"
     misc_feature    2409..2528
                     /label=PEST
                     /note="/vntifkey=21"
     misc_feature    2535..2619
                     /label=beta-globin exon component
                     /label=beta-globin\exon\component
                     /note="/vntifkey=21"
     misc_feature    2691..2930
                     /label=3MS2
                     /note="/vntifkey=21"
     misc_feature    2711..2729
                     /label=ms2 (1/6)
                     /label=ms2\(1/6)
                     /note="/vntifkey=21"
     misc_feature    2906..2924
                     /label=ms2 (6/6)
                     /label=ms2\(6/6)
                     /note="/vntifkey=21"
     promoter        complement(2947..2964)
                     /label=SP6
                     /note="/vntifkey=30"
     polyA_site      2966..3197
                     /label=BGHpolyA
                     /note="/vntifkey=26"
     misc_feature    2990..3214
                     /label=BGHpolyA ?
                     /label=BGHpolyA\?
                     /note="/vntifkey=21"
     promoter        3738..4063
                     /label=pSV40
                     /note="/vntifkey=29"
     rep_origin      3932..4017
                     /direction=RIGHT
                     /label=SV40 ori
                     /label=SV40\ori
                     /note="/vntifkey=33"
     CDS             4099..4893
                     /codon_start=1
                     /label=Neomycin
                     /note="/vntifkey=4"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_site      4948..5320
                     /label=SV40 polyA
                     /label=SV40\polyA
                     /note="/vntifkey=26"
     rep_origin      5580..6253
                     /direction=RIGHT
                     /label=ColEI
                     /note="/vntifkey=33"
     CDS             complement(6398..7258)
                     /codon_start=1
                     /label=Ampicilin
                     /note="/vntifkey=4"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtgcgcaaa
      241 tttaaccctc actaaaggga acaaaagctg ggtaccgggc cccccctcga gtttaccact
      301 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
      361 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt
      421 accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga
      481 tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
      541 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagctcgg tacccgggtc
      601 gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg aaccgtcaga
      661 tcgcctggag acgccatcca cgctgaagct tagccaccat ggccgatgct aagaacatta
      721 agaagggccc tgctcccttc taccctctgg aggatggcac cgctggcgag cagctgcaca
      781 aggccatgaa gaggtatgcc ctggtgcctg gcaccattgc cttcaccgat gcccacattg
      841 aggtggacat cacctatgcc gagtacttcg agatgtctgt gcgcctggcc gaggccatga
      901 agaggtacgg cctgaacacc aaccaccgca tcgtggtgtg ctctgagaac tctctgcagt
      961 tcttcatgcc agtgctgggc gccctgttca tcggagtggc cgtggcccct gctaacgaca
     1021 tttacaacga gcgcgagctg ctgaacagca tgggcatttc tcagcctacc gtggtgttcg
     1081 tgtctaagaa gggcctgcag aagatcctga acgtgcagaa gaagctgcct atcatccaga
     1141 agatcatcat catggactct aagaccgact accagggctt ccagagcatg tacacattcg
     1201 tgacatctca tctgcctcct ggcttcaacg agtacgactt cgtgccagag tctttcgaca
     1261 gggacaaaac cattgccctg atcatgaaca gctctgggtc taccggcctg cctaagggcg
     1321 tggccctgcc tcatcgcacc gcctgtgtgc gcttctctca cgcccgcgac cctattttcg
     1381 gcaaccagat catccccgac accgctattc tgagcgtggt gccattccac cacggcttcg
     1441 gcatgttcac caccctgggc tacctgattt gcggctttcg ggtggtgctg atgtaccgct
     1501 tcgaggagga gctgttcctg cgcagcctgc aagactacaa aattcagtct gccctgctgg
     1561 tgccaaccct gttcagcttc ttcgctaaga gcaccctgat cgacaagtac gacctgtcta
     1621 acctgcacga gattgcctct ggcggcgccc cactgtctaa ggaggtgggc gaagccgtgg
     1681 ccaagcgctt tcatctgcca ggcatccgcc agggctacgg cctgaccgag acaaccagcg
     1741 ccattctgat taccccagag ggcgacgaca agcctggcgc cgtgggcaag gtggtgccat
     1801 tcttcgaggc caaggtggtg gacctggaca ccggcaagac cctgggagtg aaccagcgcg
     1861 gcgagctgtg tgtgcgcggc cctatgatta tgtccggcta cgtgaataac cctgaggcca
     1921 caaacgccct gatcgacaag gacggctggc tgcactctgg cgacattgcc tactgggacg
     1981 aggacgagca cttcttcatc gtggaccgcc tgaagtctct gatcaagtac aagggctacc
     2041 aggtggcccc agccgagctg gagtctatcc tgctgcagca ccctaacatt ttcgacgccg
     2101 gagtggccgg cctgcccgac gacgatgccg gcgagctgcc tgccgccgtc gtcgtgctgg
     2161 aacacggcaa gaccatgacc gagaaggaga tcgtggacta tgtggccagc caggtgacaa
     2221 ccgccaagaa gctgcgcggc ggagtggtgt tcgtggacga ggtgcccaag ggcctgaccg
     2281 gcaagctgga cgcccgcaag atccgcgaga tcctgatcaa ggctaagaaa ggcggcaaga
     2341 tcgccgtgaa ttctgcttgc aagaactggt tcagtagctt aagccacttt gtgatccacc
     2401 ttaacagcca cggcttccct cccgaggtgg aggagcaggc cgccggcacc ctgcccatga
     2461 gctgcgccca ggagagcggc atggatagac accctgctgc ttgcgccagc gccaggatca
     2521 acgtctaata gtgagaattc accccaccag tgcaggctgc ctatcagaaa gtggtggctg
     2581 gtgtggctaa tgccctggcc cacaagtatc actaagcggc cgctcgcttt cttgctgtcc
     2641 aatttctatt aaaggttcct ttgttcccta agtccaacta ccaaactggg tctagctcta
     2701 gctgtagaaa acatgaggat cacccatgtc tgctggacga ctgtagaaaa catgaggatc
     2761 acccatgtct gctgtctagc tgtagaaaac atgaggatca cccatgtctg ctggacgact
     2821 gtagaaaaca tgaggatcac ccatgtctgc tgtctagctg tagaaaacat gaggatcacc
     2881 catgtctgct ggacgactgt agaaaacatg aggatcaccc atgtctgctg tctagagggc
     2941 cctattctat agtgtcacct aaatgctaga gctcgctgat cagcctcgac tgtgccttct
     3001 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     3061 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     3121 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     3181 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     3241 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     3301 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     3361 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcggggcatc
     3421 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     3481 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     3541 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     3601 gtctattctt ttgatttata agggattttg gggatttcgg cctattggtt aaaaaatgag
     3661 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
     3721 gaaagtcccc aggctcccca ggcaggcaga agtatgcaaa gcatgcatct caattagtca
     3781 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     3841 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     3901 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     3961 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     4021 ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga tcaagagaca
     4081 ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct
     4141 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
     4201 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
     4261 ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc
     4321 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
     4381 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
     4441 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
     4501 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
     4561 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
     4621 aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
     4681 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
     4741 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
     4801 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
     4861 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg
     4921 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa
     4981 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
     5041 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat
     5101 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     5161 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga
     5221 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     5281 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     5341 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     5401 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     5461 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     5521 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     5581 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     5641 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     5701 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     5761 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     5821 tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     5881 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     5941 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     6001 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     6061 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     6121 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     6181 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     6241 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     6301 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     6361 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     6421 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     6481 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     6541 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     6601 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     6661 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     6721 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     6781 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     6841 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     6901 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     6961 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     7021 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     7081 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     7141 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     7201 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     7261 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     7321 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     7381 tgccacctga cgtc
//
