LOCUS       pTET2-RlucCP-3MS        6677 bp DNA     circular SYN 03-FEB-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pcTET2-BwtB2-3MS2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6677)
  AUTHORS   Suzanne Lee
  TITLE     Direct Submission
  JOURNAL   Exported Feb 9, 2023 from SnapGene 6.2.1
            https://www.snapgene.com
COMMENT     ORIGDB|GenBank
FEATURES             Location/Qualifiers
     source          1..6677
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        234..685
                     /label=pTET2
                     /note="/vntifkey=29"
     misc_feature    633..654
                     /label=pTet sequencing primer
                     /label=pTet\sequencing\primer
                     /note="/vntifkey=21"
     misc_feature    699..1811
                     /label=hRlucCP
                     /note="/vntifkey=21"
     misc_feature    699..1631
                     /label=Rluc
                     /note="/vntifkey=21"
     misc_feature    803..1634
                     /label=alighn, 20130717 CP1R clone 8
                     /label=alighn,\20130717\CP1R\clone\8
                     /label=nonstandard type:  insertion_seq
                     /note="/vntifkey=14"
     misc_binding    1568..1587
                     /label=Renilla XcmI F
                     /label=Renilla\XcmI\F
                     /note="/vntifkey=20"
     primer_bind     1568..1587
                     /label=qPCR.FOR
     misc_feature    1610..2420
                     /label=align, 20130717 Renilla F clone 8
                     /label=align,\20130717\Renilla\F\clone\8
                     /label=nonstandard type:  insertion_seq
                     /note="/vntifkey=14"
     misc_feature    1632..1691
                     /label=CP1
                     /note="/vntifkey=21"
     primer_bind     complement(1663..1683)
                     /label=qPCR.REV
     misc_feature    1692..1811
                     /label=PEST
                     /note="/vntifkey=21"
     misc_feature    1818..1902
                     /label=beta globin exon component
                     /label=beta\globin\exon\component
                     /note="/vntifkey=21"
     misc_difference 1898
                     /label=A to C?
                     /label=A\to\C?
                     /note="/vntifkey=85"
     misc_feature    1974..2213
                     /label=3MS2
                     /note="/vntifkey=21"
     misc_feature    1994..2012
                     /label=ms2 (1/6)
                     /label=ms2\(1/6)
                     /note="/vntifkey=21"
     misc_feature    2189..2207
                     /label=ms2 (6/6)
                     /label=ms2\(6/6)
                     /note="/vntifkey=21"
     promoter        complement(2230..2247)
                     /label=SP6
                     /note="/vntifkey=30"
     polyA_site      2249..2480
                     /label=BGHpolyA
                     /note="/vntifkey=26"
     misc_feature    2273..2497
                     /label=BGHpolyA?
                     /note="/vntifkey=21"
     promoter        3021..3346
                     /label=pSV40
                     /note="/vntifkey=29"
     rep_origin      3215..3300
                     /direction=RIGHT
                     /label=SV40 ori
                     /label=SV40\ori
                     /note="/vntifkey=33"
     CDS             3382..4176
                     /codon_start=1
                     /label=Neomycin
                     /note="/vntifkey=4"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_site      4231..4603
                     /label=SV40 polyA
                     /label=SV40\polyA
                     /note="/vntifkey=26"
     rep_origin      4863..5536
                     /direction=RIGHT
                     /label=ColEI
                     /note="/vntifkey=33"
     CDS             complement(5681..6541)
                     /codon_start=1
                     /label=Ampicilin
                     /note="/vntifkey=4"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtgcgcaaa
      241 tttaaccctc actaaaggga acaaaagctg ggtaccgggc cccccctcga gtttaccact
      301 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
      361 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt
      421 accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga
      481 tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
      541 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagctcgg tacccgggtc
      601 gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg aaccgtcaga
      661 tcgcctggag acgccatcca cgctgaagct tagccaccat ggcttccaag gtgtacgacc
      721 ccgagcaacg caaacgcatg atcactgggc ctcagtggtg ggctcgctgc aagcaaatga
      781 acgtgctgga ctccttcatc aactactatg attccgagaa gcacgccgag aacgccgtga
      841 tttttctgca tggtaacgct gcctccagct acctgtggag gcacgtcgtg cctcacatcg
      901 agcccgtggc tagatgcatc atccctgatc tgatcggaat gggtaagtcc ggcaagagcg
      961 ggaatggctc atatcgcctc ctggatcact acaagtacct caccgcttgg ttcgagctgc
     1021 tgaaccttcc aaagaaaatc atctttgtgg gccacgactg gggggcttgt ctggcctttc
     1081 actactccta cgagcaccaa gacaagatca aggccatcgt ccatgctgag agtgtcgtgg
     1141 acgtgatcga gtcctgggac gagtggcctg acatcgagga ggatatcgcc ctgatcaaga
     1201 gcgaagaggg cgagaaaatg gtgcttgaga ataacttctt cgtcgagacc atgctcccaa
     1261 gcaagatcat gcggaaactg gagcctgagg agttcgctgc ctacctggag ccattcaagg
     1321 agaagggcga ggttagacgg cctaccctct cctggcctcg cgagatccct ctcgttaagg
     1381 gaggcaagcc cgacgtcgtc cagattgtcc gcaactacaa cgcctacctt cgggccagcg
     1441 acgatctgcc taagatgttc atcgagtccg accctgggtt cttttccaac gctattgtcg
     1501 agggagctaa gaagttccct aacaccgagt tcgtgaaggt gaagggcctc cacttcagcc
     1561 aggaggacgc tccagatgaa atgggtaagt acatcaagag cttcgtggag cgcgtgctga
     1621 agaacgagca gaattctgct tgcaagaact ggttcagtag cttaagccac tttgtgatcc
     1681 accttaacag ccacggcttc cctcccgagg tggaggagca ggccgccggc accctgccca
     1741 tgagctgcgc ccaggagagc ggcatggata gacaccctgc tgcttgcgcc agcgccagga
     1801 tcaacgtcta atagtgagaa ttcaccccac cagtgcaggc tgcctatcag aaagtggtgg
     1861 ctggtgtggc taatgccctg gcccacaagt atcactacgc ggccgctcgc tttcttgctg
     1921 tccaatttct attaaaggtt cctttgttcc ctaagtccaa ctaccaaact gggtctagct
     1981 ctagctgtag aaaacatgag gatcacccat gtctgctgga cgactgtaga aaacatgagg
     2041 atcacccatg tctgctgtct agctgtagaa aacatgagga tcacccatgt ctgctggacg
     2101 actgtagaaa acatgaggat cacccatgtc tgctgtctag ctgtagaaaa catgaggatc
     2161 acccatgtct gctggacgac tgtagaaaac atgaggatca cccatgtctg ctgtctagag
     2221 ggccctattc tatagtgtca cctaaatgct agagctcgct gatcagcctc gactgtgcct
     2281 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     2341 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     2401 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     2461 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
     2521 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     2581 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     2641 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggc
     2701 atccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     2761 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     2821 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     2881 tcggtctatt cttttgattt ataagggatt ttggggattt cggcctattg gttaaaaaat
     2941 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt
     3001 gtggaaagtc cccaggctcc ccaggcaggc agaagtatgc aaagcatgca tctcaattag
     3061 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     3121 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     3181 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     3241 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     3301 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag
     3361 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc
     3421 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     3481 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     3541 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg
     3601 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     3661 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     3721 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     3781 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     3841 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     3901 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
     3961 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     4021 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     4081 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     4141 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga
     4201 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg
     4261 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     4321 atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca
     4381 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     4441 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct
     4501 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     4561 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     4621 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     4681 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     4741 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     4801 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     4861 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     4921 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     4981 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     5041 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     5101 gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     5161 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     5221 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     5281 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     5341 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     5401 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     5461 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     5521 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     5581 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     5641 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     5701 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     5761 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     5821 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     5881 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     5941 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     6001 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     6061 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     6121 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     6181 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     6241 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     6301 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     6361 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     6421 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     6481 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     6541 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     6601 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     6661 aagtgccacc tgacgtc
//
