LOCUS       pB-M.CviPII-NDel15-ZZ-Strep      5514 bp    DNA     circular UNA 18-6-2020
DEFINITION  
FEATURES             Location/Qualifiers
     promoter        4..276
                     /label="araBAD"
     primer          9..33
                     /label="araBAD-F"
     primer          199..223
                     /label="araBAD-F2"
     primer          complement(294..318)
                     /label="araBAD-R"
     CDS             319..1881
                     /cds_type="ORF"
     Memo            331..348
                     /label="His6"
     PeptideTag      331..348
                     /label="His6"
     Memo            397..411
                     /label="DDDDK-tag"
     gene            424..1467
                     /label="M.CviPII-NDel15"
     gene            1474..1851
                     /label="ZZ"
     misc_feature    1852..1878
                     /feature_type="Protein Tag"
                     /label="Strep Tag"
     feature         1964..2121
                     /label="rrmB translation termination signal"
     primer          complement(1977..2001)
                     /label="araBAD-R2"
     gene            2401..3261
                     /label="AmpR"
     gene            4610..5478
                     /label="AraC Orf"
ORIGIN
        1 AAGAAACCAA TTGTCCATAT TGCATCAGAC ATTGCCGTCA CTGCGTCTTT TACTGGCTCT
       61 TCTCGCTAAC CAAACCGGTA ACCCCGCTTA TTAAAAGCAT TCTGTAACAA AGCGGGACCA
      121 AAGCCATGAC AAAAACGCGT AACAAAAGTG TCTATAATCA CGGCAGAAAA GTCCACATTG
      181 ATTATTTGCA CGGCGTCACA CTTTGCTATG CCATAGCATT TTTATCCATA AGATTAGCCG
      241 ATCCTACCTG ACGCTTTTTA TCGCAACTCT CTACTGTTTC TCCATACCCG TTTTTTGGGC
      301 TAACAGGAGG AATTAACCAT GGGGGGTTCT CATCATCATC ATCATCATGG TATGGCTAGC
      361 ATGACTGGTG GACAGCAAAT GGGTCGGGAT CTGTACGACG ATGACGATAA GCTCGAGGGA
      421 TCCatgaaag ccttggaact atttgcgggg ataggtggca ttacccatgg cttacgtggc
      481 tatgtagaac cgattgcatt ctgcgaatat gagaaagatg ccagctcgtt tctgtcacaa
      541 cgtggcttac ccgttcatgg ggacattacg aagtttgacg ccagtgtgta caaaaacaag
      601 attgacattg tgacagcagg atggccttgt actgggtttt cgaccgcggg taaaggcact
      661 ggttttgagc atgaagctag cggtttatgg acagaagtgg tccgagtagt gaaagagagc
      721 gaaccgaaat acgtcttttt ggaaaatagt cacgttttgg cgcaaaccaa aaacctcaag
      781 gtcatcatcc atgatctgga tatccttggc tatgatacgc gttggtggac gtgcagatcg
      841 aatgacgtta atgtcggtgc acatcacaat cggtatcgct ggtttatgct cgcagagaaa
      901 aaaggctctg tgaccaaatt cgtgaaaatt caggtgaaaa agttcaattg gtctggtgac
      961 ttcaaagaga aacagataag cgagaacagt cacgaaaaca agcagctcat taagttcatg
     1021 ggaaactccg tagttccgga tcaagttcgc tatgcgtttg aatcgatgag cgacatgatt
     1081 ctggaaggca gtctggtgaa tgataaggat gagatcgtga aggtaggcta ttccaaagat
     1141 ggcatcatgt acaaaattcc gattgaacac aaaatcattc cgaaacttaa catcgttctg
     1201 accccacgtg atccgccaga aggccataaa gcccgtgaag aagcgatcat caaaagcccc
     1261 attctgatga cgtactggaa tactccggcc ttttgctatc acaaatcagc tcgcggcgct
     1321 aaaattctga ccaaacgtca gaaaaacaac ctgcataccc agatcaaatt ctgtcctggt
     1381 ggttctgacg atggttatct gtcaggtcgc ttttgtgcgt ggctgatggg atacgatgat
     1441 gaatacctgg gggatcttat gcactatGAA TTCCAGCATG ATGAAGCAGT GGATAACAAG
     1501 TTTAACAAAG AACAGCAGAA CGCGTTTTAT GAGATCTTGC ACTTACCGAA TCTGAACGAG
     1561 GAACAGCGCA ATGCGTTCAT TCAGTCCCTT AAAGACGATC CATCTCAAAG CGCAAATCTG
     1621 CTGGCAGAAG CCAAGAAACT GAACGATGCT CAAGCTCCGA AAGTCGACAA CAAATTCAAC
     1681 AAAGAGCAAC AGAATGCGTT CTACGAAATT CTGCATTTAC CGAACCTGAA TGAAGAACAG
     1741 CGTAACGCCT TTATCCAATC GCTGAAAGAT GATCCTAGTC AGTCAGCCAA TCTGTTGGCG
     1801 GAAGCGAAGA AACTCAATGA CGCCCAAGCT CCCAAAGTTG ACGCGAATAG CTGGAGCCAT
     1861 CCGCAGTTTG AAAAGCTTTA GGGCTGTTTT GGCGGATGAG AGAAGATTTT CAGCCTGATA
     1921 CAGATTAAAT CAGAACGCAG AAGCGGTCTG ATAAAACAGA ATTTGCCTGG CGGCAGTAGC
     1981 GCGGTGGTCC CACCTGACCC CATGCCGAAC TCAGAAGTGA AACGCCGTAG CGCCGATGGT
     2041 AGTGTGGGGT CTCCCCATGC GAGAGTAGGG AACTGCCAGG CATCAAATAA AACGAAAGGC
     2101 TCAGTCGAAA GACTGGGCCT TTCGTTTTAT CTGTTGTTTG TCGGTGAACG CTCTCCTGAG
     2161 TAGGACAAAT CCGCCGGGAG CGGATTTGAA CGTTGCGAAG CAACGGCCCG GAGGGTGGCG
     2221 GGCAGGACGC CCGCCATAAA CTGCCAGGCA TCAAATTAAG CAGAAGGCCA TCCTGACGGA
     2281 TGGCCTTTTT GCGTTTCTAC AAACTCTTTT TGTTTATTTT TCTAAATACA TTCAAATATG
     2341 TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT
     2401 ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT
     2461 GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA
     2521 CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC
     2581 GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC
     2641 CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG
     2701 GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA
     2761 TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC
     2821 GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT
     2881 GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG
     2941 CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT
     3001 TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC
     3061 TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT
     3121 CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC
     3181 ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC
     3241 TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT
     3301 TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG
     3361 ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC
     3421 AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA
     3481 CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG
     3541 GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA
     3601 GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA
     3661 CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG
     3721 TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG
     3781 GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG
     3841 CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG
     3901 CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC
     3961 CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA
     4021 AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG
     4081 TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT
     4141 GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA
     4201 GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATGG
     4261 TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT
     4321 CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT
     4381 GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT
     4441 GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCAG ATCAATTCGC
     4501 GCGCGAAGGC GAAGCGGCAT GCATAATGTG CCTGTCAAAT GGACGAAGCA GGGATTCTGC
     4561 AAACCCTATG CTACTCCGTC AAGCCGTCAA TTGTCTGATT CGTTACCAAT TATGACAACT
     4621 TGACGGCTAC ATCATTCACT TTTTCTTCAC AACCGGCACG GAACTCGCTC GGGCTGGCCC
     4681 CGGTGCATTT TTTAAATACC CGCGAGAAAT AGAGTTGATC GTCAAAACCA ACATTGCGAC
     4741 CGACGGTGGC GATAGGCATC CGGGTGGTGC TCAAAAGCAG CTTCGCCTGG CTGATACGTT
     4801 GGTCCTCGCG CCAGCTTAAG ACGCTAATCC CTAACTGCTG GCGGAAAAAA TGTGACAGAC
     4861 GCGACGGCGA CAAGCAAACA TGCTGTGCGA CGCTGGCGAT ATCAAAATTG CTGTCTGCCA
     4921 GGTGATCGCT GATGTACTGA CAAGCCTCGC GTACCCGATT ATCCATCGGT GGATGGAGCG
     4981 ACTCGTTAAT CGCTTCCATG CGCCGCAGTA ACAATTGCTC AAGCAGATTT ATCGCCAGCA
     5041 GCTCCGAATA GCGCCCTTCC CCTTGCCCGG CGTTAATGAT TTGCCCAAAC AGGTCGCTGA
     5101 AATGCGGCTG GTGCGCTTCA TCCGGGCGAA AGAACCCCGT ATTGGCAAAT ATTGACGGCC
     5161 AGTTAAGCCA TTCATGCCAG TAGGCGCGCG GACGAAAGTA AACCCACTGG TGATACCATT
     5221 CGCGAGCCTC CGGATGACGA CCGTAGTGAT GAATCTCTCC TGGCGGGAAC AGCAAAATAT
     5281 CACCCGGTCG GCAAACAAAT TCTCGTCCCT GATTTTTCAC CACCCCCTGA CCGCGAATGG
     5341 TGAGATTGAG AATATAACCT TTCATTCCCA GCGGTCGGTC GATAAAAAAA TCGAGATAAC
     5401 CGTTGGCCTC AATCGGCGTT AAACCCGCCA CCAGATGGGC ATTAAACGAG TATCCCGGCA
     5461 GCAGGGGATC ATTTTGCGCT TCAGCCATAC TTTTCATACT CCCGCCATTC AGAG
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