LOCUS       pB-M.CviQIX-NDel15-ZZ-Strep      5520 bp    DNA     circular UNA 18-6-2020
DEFINITION  
FEATURES             Location/Qualifiers
     promoter        4..276
                     /label="araBAD"
     primer          9..33
                     /label="araBAD-F"
     primer          199..223
                     /label="araBAD-F2"
     primer          complement(294..318)
                     /label="araBAD-R"
     CDS             319..1887
                     /cds_type="ORF"
     Memo            331..348
                     /label="His6"
     PeptideTag      331..348
                     /label="His6"
     Memo            397..411
                     /label="DDDDK-tag"
     gene            424..1473
     gene            1480..1857
                     /label="ZZ"
     misc_feature    1858..1884
                     /feature_type="Protein Tag"
                     /label="Strep Tag"
     feature         1970..2127
                     /label="rrmB translation termination signal"
     primer          complement(1983..2007)
                     /label="araBAD-R2"
     gene            2407..3267
                     /label="AmpR"
     gene            4616..5484
                     /label="AraC Orf"
ORIGIN
        1 AAGAAACCAA TTGTCCATAT TGCATCAGAC ATTGCCGTCA CTGCGTCTTT TACTGGCTCT
       61 TCTCGCTAAC CAAACCGGTA ACCCCGCTTA TTAAAAGCAT TCTGTAACAA AGCGGGACCA
      121 AAGCCATGAC AAAAACGCGT AACAAAAGTG TCTATAATCA CGGCAGAAAA GTCCACATTG
      181 ATTATTTGCA CGGCGTCACA CTTTGCTATG CCATAGCATT TTTATCCATA AGATTAGCCG
      241 ATCCTACCTG ACGCTTTTTA TCGCAACTCT CTACTGTTTC TCCATACCCG TTTTTTGGGC
      301 TAACAGGAGG AATTAACCAT GGGGGGTTCT CATCATCATC ATCATCATGG TATGGCTAGC
      361 ATGACTGGTG GACAGCAAAT GGGTCGGGAT CTGTACGACG ATGACGATAA GCTCGAGGGA
      421 TCCatgtcca tgaaagcctt ggaactattt gcggggatag gtggcattac ccatggctta
      481 cgtggctatg tagaaccgat tgcattctgc gaatatgaga aagatgccag ctcgtttctg
      541 tcacaacgtg gcttacccgt tcatggggac attacgaagt ttgacgccag tgtgtacaaa
      601 aacaagattg acattgtgac agcaggatgg ccttgtactg ggttttcgac cgcgggtaaa
      661 ggcactggtt ttgagcatga agctagcggt ttatggacag aagtggtccg agtagtgaaa
      721 gagagcgaac cgaaatacgt ctttttggaa aatagtcacg ttttggcgca aaccaaaaac
      781 ctcaaggtca tcatccatga tctggataac cttggctatg atacgcgttg gtggacgtgc
      841 agatcgaatg acgttaatgt cggtgcacat cacaatcggt atcgctggtt tatgctcgca
      901 gagaaaaaag gctctgtgac caaattcgtg aaaattcagg tgaaaaagtt caattggtct
      961 ggtgacttca aagagaaaca gataagcgag aacagtcacg aaaacaagca gctcattaag
     1021 ttcatgggaa actccgtagt tccggatcaa gttcgctatg cgtttgaatc gatgagcgac
     1081 atgattctgg aaggcagtct ggtgaatgaa aaggatgaga tcgtgaaggt aggctattcc
     1141 aaagatagca tcatgtacaa aattccgatt gaacacaaaa tcattccgaa acttaacatc
     1201 gttctgaccc cacgtgatcc gccagaaggc cataaagccc gtgaagaagc gatcatcaaa
     1261 agccccattc tgatgacgta ctggaatact ccggcctttt gctatcacaa atcagctcgc
     1321 ggcgctaaaa ttctgaccaa acgtcagaaa aacaacctgc atacccagat caaattctgt
     1381 cctggtggtt ctgacgatgg ttatctgtca ggtcgctttt gtgcgtggct gatgggatac
     1441 gatgatgaat acctggggga tcttatgcac tatGAATTCC AGCATGATGA AGCAGTGGAT
     1501 AACAAGTTTA ACAAAGAACA GCAGAACGCG TTTTATGAGA TCTTGCACTT ACCGAATCTG
     1561 AACGAGGAAC AGCGCAATGC GTTCATTCAG TCCCTTAAAG ACGATCCATC TCAAAGCGCA
     1621 AATCTGCTGG CAGAAGCCAA GAAACTGAAC GATGCTCAAG CTCCGAAAGT CGACAACAAA
     1681 TTCAACAAAG AGCAACAGAA TGCGTTCTAC GAAATTCTGC ATTTACCGAA CCTGAATGAA
     1741 GAACAGCGTA ACGCCTTTAT CCAATCGCTG AAAGATGATC CTAGTCAGTC AGCCAATCTG
     1801 TTGGCGGAAG CGAAGAAACT CAATGACGCC CAAGCTCCCA AAGTTGACGC GAATAGCTGG
     1861 AGCCATCCGC AGTTTGAAAA GCTTTAGGGC TGTTTTGGCG GATGAGAGAA GATTTTCAGC
     1921 CTGATACAGA TTAAATCAGA ACGCAGAAGC GGTCTGATAA AACAGAATTT GCCTGGCGGC
     1981 AGTAGCGCGG TGGTCCCACC TGACCCCATG CCGAACTCAG AAGTGAAACG CCGTAGCGCC
     2041 GATGGTAGTG TGGGGTCTCC CCATGCGAGA GTAGGGAACT GCCAGGCATC AAATAAAACG
     2101 AAAGGCTCAG TCGAAAGACT GGGCCTTTCG TTTTATCTGT TGTTTGTCGG TGAACGCTCT
     2161 CCTGAGTAGG ACAAATCCGC CGGGAGCGGA TTTGAACGTT GCGAAGCAAC GGCCCGGAGG
     2221 GTGGCGGGCA GGACGCCCGC CATAAACTGC CAGGCATCAA ATTAAGCAGA AGGCCATCCT
     2281 GACGGATGGC CTTTTTGCGT TTCTACAAAC TCTTTTTGTT TATTTTTCTA AATACATTCA
     2341 AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG
     2401 AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC
     2461 CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG
     2521 GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT
     2581 CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA
     2641 TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT
     2701 GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA
     2761 GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA
     2821 ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT
     2881 CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC
     2941 ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT
     3001 CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT
     3061 CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT
     3121 GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT
     3181 ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA
     3241 GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG
     3301 ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT
     3361 CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA
     3421 AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA
     3481 AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT
     3541 CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG
     3601 TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC
     3661 CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA
     3721 CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC
     3781 AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC
     3841 GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA
     3901 GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG
     3961 TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA
     4021 TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT
     4081 CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG
     4141 TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA
     4201 GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC
     4261 ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC
     4321 CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG
     4381 CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG
     4441 GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCAGATCA
     4501 ATTCGCGCGC GAAGGCGAAG CGGCATGCAT AATGTGCCTG TCAAATGGAC GAAGCAGGGA
     4561 TTCTGCAAAC CCTATGCTAC TCCGTCAAGC CGTCAATTGT CTGATTCGTT ACCAATTATG
     4621 ACAACTTGAC GGCTACATCA TTCACTTTTT CTTCACAACC GGCACGGAAC TCGCTCGGGC
     4681 TGGCCCCGGT GCATTTTTTA AATACCCGCG AGAAATAGAG TTGATCGTCA AAACCAACAT
     4741 TGCGACCGAC GGTGGCGATA GGCATCCGGG TGGTGCTCAA AAGCAGCTTC GCCTGGCTGA
     4801 TACGTTGGTC CTCGCGCCAG CTTAAGACGC TAATCCCTAA CTGCTGGCGG AAAAAATGTG
     4861 ACAGACGCGA CGGCGACAAG CAAACATGCT GTGCGACGCT GGCGATATCA AAATTGCTGT
     4921 CTGCCAGGTG ATCGCTGATG TACTGACAAG CCTCGCGTAC CCGATTATCC ATCGGTGGAT
     4981 GGAGCGACTC GTTAATCGCT TCCATGCGCC GCAGTAACAA TTGCTCAAGC AGATTTATCG
     5041 CCAGCAGCTC CGAATAGCGC CCTTCCCCTT GCCCGGCGTT AATGATTTGC CCAAACAGGT
     5101 CGCTGAAATG CGGCTGGTGC GCTTCATCCG GGCGAAAGAA CCCCGTATTG GCAAATATTG
     5161 ACGGCCAGTT AAGCCATTCA TGCCAGTAGG CGCGCGGACG AAAGTAAACC CACTGGTGAT
     5221 ACCATTCGCG AGCCTCCGGA TGACGACCGT AGTGATGAAT CTCTCCTGGC GGGAACAGCA
     5281 AAATATCACC CGGTCGGCAA ACAAATTCTC GTCCCTGATT TTTCACCACC CCCTGACCGC
     5341 GAATGGTGAG ATTGAGAATA TAACCTTTCA TTCCCAGCGG TCGGTCGATA AAAAAATCGA
     5401 GATAACCGTT GGCCTCAATC GGCGTTAAAC CCGCCACCAG ATGGGCATTA AACGAGTATC
     5461 CCGGCAGCAG GGGATCATTT TGCGCTTCAG CCATACTTTT CATACTCCCG CCATTCAGAG
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