LOCUS       pB-NP_049039-ZZ-Strep      5571 bp    DNA     circular UNA 18-6-2020
DEFINITION  
FEATURES             Location/Qualifiers
     promoter        4..276
                     /label="araBAD"
     primer          9..33
                     /label="araBAD-F"
     primer          199..223
                     /label="araBAD-F2"
     primer          complement(294..318)
                     /label="araBAD-R"
     CDS             319..1938
                     /cds_type="ORF"
     Memo            331..348
                     /label="His6"
     PeptideTag      331..348
                     /label="His6"
     Memo            397..411
                     /label="DDDDK-tag"
     gene            424..1524
     gene            1531..1908
                     /label="ZZ"
     misc_feature    1909..1935
                     /feature_type="Protein Tag"
                     /label="Strep Tag"
     feature         2021..2178
                     /label="rrmB translation termination signal"
     primer          complement(2034..2058)
                     /label="araBAD-R2"
     gene            2458..3318
                     /label="AmpR"
     gene            4667..5535
                     /label="AraC Orf"
ORIGIN
        1 AAGAAACCAA TTGTCCATAT TGCATCAGAC ATTGCCGTCA CTGCGTCTTT TACTGGCTCT
       61 TCTCGCTAAC CAAACCGGTA ACCCCGCTTA TTAAAAGCAT TCTGTAACAA AGCGGGACCA
      121 AAGCCATGAC AAAAACGCGT AACAAAAGTG TCTATAATCA CGGCAGAAAA GTCCACATTG
      181 ATTATTTGCA CGGCGTCACA CTTTGCTATG CCATAGCATT TTTATCCATA AGATTAGCCG
      241 ATCCTACCTG ACGCTTTTTA TCGCAACTCT CTACTGTTTC TCCATACCCG TTTTTTGGGC
      301 TAACAGGAGG AATTAACCAT GGGGGGTTCT CATCATCATC ATCATCATGG TATGGCTAGC
      361 ATGACTGGTG GACAGCAAAT GGGTCGGGAT CTGTACGACG ATGACGATAA GCTCGAGGGA
      421 TCCATGTCAT TTAGGACATT AGAACTATTC GCTGGCATCG CCGGTATCAG CCACGGCCTG
      481 CGTGGTATTT CCACCCCGGT TGCCTTCGTG GAAATCAACG AAGATGCGCA AAAATTCCTG
      541 AAAACGAAGT TCTCAGACGC GAGCGTGTTC AACGACGTGA CCAAATTTAC GAAGAGCGAC
      601 TTCCCGGAGG ACATCGATAT GATTACTGCG GGTTTTCCGT GTACCGGCTT CTCGATCGCT
      661 GGCAGCCGTA CCGGCTTCGA GCACAAAGAG TCCGGTCTGT TTGCTGATGT GGTGCGTATT
      721 ACCGAGGAAT ATAAACCGAA GATCGTGTTT TTGGAGAACT CGCACATGCT GAGCCACACC
      781 TACAATCTGG ATGTAGTCGT CAAAAAGATG GATGAAATTG GTTACTTCTG CAAGTGGGTT
      841 ACCTGTCGTG CGAGCATTGT TGGTGCACAT CATCAGCGTC ACCGCTGGTT TTGCCTGGCG
      901 ATCCGCAAGG ACTATGAACC GAAAGAGATC ATCGTTAGCG TGAATGCCAC CAAGTTCGAT
      961 TGGGAAAACA ACGAGCCGCC TTGCAAGGTT GATAATAAAT CTTACGAGAA CAGCACATTG
     1021 GTACGCCTGG CTGGCTATAG CGTGGTTCCG GATCAAATTC GTTATGCGTT TACCGGCCTC
     1081 TTCACCGGTG ATTTTGAATC CAGCTGGAAA ACGACTTTAA CCCCAGGTGC GATTAACGGT
     1141 ACTGAGCACA AGAAGATGAA AGGTACGTAC GACAAGGTGA TTAACGGCTA CTACGAGAAC
     1201 GACGTGTATT ACTCCTTTTC CCGCAAAGAG GTTCATAGAG CACCGCTGAA TATTTCTGTC
     1261 AAGCCGCGTG ACATTCCGGA AAAACACAAC GGTAAAACTT TGGTCGACCG TGAAATGATC
     1321 AAAAAGTATT GGTGCACCCC GTGCGCGAGC TACGGCACGG CTACCGCGGG TTGCAATGTT
     1381 CTGACCGATC GTCAGAGCCA TGCACTGCCG ACCCAGGTTC GTTTTTCTTA CCGCGGAGTT
     1441 TGTGGTCGTC ATCTGAGCGG CATCTGGTGC GCATGGCTGA TGGGTTACGA CCAAGAATAC
     1501 CTAAGCTATT TGGTGCAGTA TGACGAATTC CAGCATGATG AAGCAGTGGA TAACAAGTTT
     1561 AACAAAGAAC AGCAGAACGC GTTTTATGAG ATCTTGCACT TACCGAATCT GAACGAGGAA
     1621 CAGCGCAATG CGTTCATTCA GTCCCTTAAA GACGATCCAT CTCAAAGCGC AAATCTGCTG
     1681 GCAGAAGCCA AGAAACTGAA CGATGCTCAA GCTCCGAAAG TCGACAACAA ATTCAACAAA
     1741 GAGCAACAGA ATGCGTTCTA CGAAATTCTG CATTTACCGA ACCTGAATGA AGAACAGCGT
     1801 AACGCCTTTA TCCAATCGCT GAAAGATGAT CCTAGTCAGT CAGCCAATCT GTTGGCGGAA
     1861 GCGAAGAAAC TCAATGACGC CCAAGCTCCC AAAGTTGACG CGAATAGCTG GAGCCATCCG
     1921 CAGTTTGAAA AGCTTTAGGG CTGTTTTGGC GGATGAGAGA AGATTTTCAG CCTGATACAG
     1981 ATTAAATCAG AACGCAGAAG CGGTCTGATA AAACAGAATT TGCCTGGCGG CAGTAGCGCG
     2041 GTGGTCCCAC CTGACCCCAT GCCGAACTCA GAAGTGAAAC GCCGTAGCGC CGATGGTAGT
     2101 GTGGGGTCTC CCCATGCGAG AGTAGGGAAC TGCCAGGCAT CAAATAAAAC GAAAGGCTCA
     2161 GTCGAAAGAC TGGGCCTTTC GTTTTATCTG TTGTTTGTCG GTGAACGCTC TCCTGAGTAG
     2221 GACAAATCCG CCGGGAGCGG ATTTGAACGT TGCGAAGCAA CGGCCCGGAG GGTGGCGGGC
     2281 AGGACGCCCG CCATAAACTG CCAGGCATCA AATTAAGCAG AAGGCCATCC TGACGGATGG
     2341 CCTTTTTGCG TTTCTACAAA CTCTTTTTGT TTATTTTTCT AAATACATTC AAATATGTAT
     2401 CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG
     2461 AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT
     2521 TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA
     2581 GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA
     2641 GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT
     2701 GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT
     2761 GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC
     2821 AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA
     2881 GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT
     2941 CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT
     3001 GTAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC
     3061 CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG
     3121 GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC
     3181 GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG
     3241 ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA
     3301 CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA
     3361 AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC
     3421 AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA
     3481 GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA
     3541 CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA
     3601 ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC
     3661 CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA
     3721 GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA
     3781 CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG
     3841 CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT
     3901 CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC
     3961 ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC
     4021 CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC
     4081 GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC
     4141 TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT
     4201 ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG
     4261 CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATGGTGC
     4321 ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC
     4381 TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC
     4441 GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA
     4501 TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCAGATC AATTCGCGCG
     4561 CGAAGGCGAA GCGGCATGCA TAATGTGCCT GTCAAATGGA CGAAGCAGGG ATTCTGCAAA
     4621 CCCTATGCTA CTCCGTCAAG CCGTCAATTG TCTGATTCGT TACCAATTAT GACAACTTGA
     4681 CGGCTACATC ATTCACTTTT TCTTCACAAC CGGCACGGAA CTCGCTCGGG CTGGCCCCGG
     4741 TGCATTTTTT AAATACCCGC GAGAAATAGA GTTGATCGTC AAAACCAACA TTGCGACCGA
     4801 CGGTGGCGAT AGGCATCCGG GTGGTGCTCA AAAGCAGCTT CGCCTGGCTG ATACGTTGGT
     4861 CCTCGCGCCA GCTTAAGACG CTAATCCCTA ACTGCTGGCG GAAAAAATGT GACAGACGCG
     4921 ACGGCGACAA GCAAACATGC TGTGCGACGC TGGCGATATC AAAATTGCTG TCTGCCAGGT
     4981 GATCGCTGAT GTACTGACAA GCCTCGCGTA CCCGATTATC CATCGGTGGA TGGAGCGACT
     5041 CGTTAATCGC TTCCATGCGC CGCAGTAACA ATTGCTCAAG CAGATTTATC GCCAGCAGCT
     5101 CCGAATAGCG CCCTTCCCCT TGCCCGGCGT TAATGATTTG CCCAAACAGG TCGCTGAAAT
     5161 GCGGCTGGTG CGCTTCATCC GGGCGAAAGA ACCCCGTATT GGCAAATATT GACGGCCAGT
     5221 TAAGCCATTC ATGCCAGTAG GCGCGCGGAC GAAAGTAAAC CCACTGGTGA TACCATTCGC
     5281 GAGCCTCCGG ATGACGACCG TAGTGATGAA TCTCTCCTGG CGGGAACAGC AAAATATCAC
     5341 CCGGTCGGCA AACAAATTCT CGTCCCTGAT TTTTCACCAC CCCCTGACCG CGAATGGTGA
     5401 GATTGAGAAT ATAACCTTTC ATTCCCAGCG GTCGGTCGAT AAAAAAATCG AGATAACCGT
     5461 TGGCCTCAAT CGGCGTTAAA CCCGCCACCA GATGGGCATT AAACGAGTAT CCCGGCAGCA
     5521 GGGGATCATT TTGCGCTTCA GCCATACTTT TCATACTCCC GCCATTCAGA G
//
