LOCUS       pB-YP_001497893-ZZ-Strep      5577 bp    DNA     circular UNA 18-6-2020
DEFINITION  
FEATURES             Location/Qualifiers
     promoter        4..276
                     /label="araBAD"
     primer          9..33
                     /label="araBAD-F"
     primer          199..223
                     /label="araBAD-F2"
     primer          complement(294..318)
                     /label="araBAD-R"
     CDS             319..1944
                     /cds_type="ORF"
     Memo            331..348
                     /label="His6"
     PeptideTag      331..348
                     /label="His6"
     Memo            397..411
                     /label="DDDDK-tag"
     gene            424..1530
     gene            1537..1914
                     /label="ZZ"
     misc_feature    1915..1941
                     /feature_type="Protein Tag"
                     /label="Strep Tag"
     feature         2027..2184
                     /label="rrmB translation termination signal"
     primer          complement(2040..2064)
                     /label="araBAD-R2"
     gene            2464..3324
                     /label="AmpR"
     gene            4673..5541
                     /label="AraC Orf"
ORIGIN
        1 AAGAAACCAA TTGTCCATAT TGCATCAGAC ATTGCCGTCA CTGCGTCTTT TACTGGCTCT
       61 TCTCGCTAAC CAAACCGGTA ACCCCGCTTA TTAAAAGCAT TCTGTAACAA AGCGGGACCA
      121 AAGCCATGAC AAAAACGCGT AACAAAAGTG TCTATAATCA CGGCAGAAAA GTCCACATTG
      181 ATTATTTGCA CGGCGTCACA CTTTGCTATG CCATAGCATT TTTATCCATA AGATTAGCCG
      241 ATCCTACCTG ACGCTTTTTA TCGCAACTCT CTACTGTTTC TCCATACCCG TTTTTTGGGC
      301 TAACAGGAGG AATTAACCAT GGGGGGTTCT CATCATCATC ATCATCATGG TATGGCTAGC
      361 ATGACTGGTG GACAGCAAAT GGGTCGGGAT CTGTACGACG ATGACGATAA GCTCGAGGGA
      421 TCCATGGTAC TAAGGGCTTT AGATTTGTTT TCAGGTATCG GCGGTATTAC CCACGGACTG
      481 CGTGAGATTG TGGAGCCGAT TGCATTCGTG GAGAAAAACG ATGAGGCGCG TAGCTTTCTG
      541 AAGAAGAAAC ATCCGGAAAT CCCGGTTTTT GACGATGTTT GTTCTTTCGA CGCGACTAAG
      601 TGGACCCATG TTGATATCAT TCTGGCTGGC TGGCCGTGTA CCGGTTTCTC TAATGCTGGC
      661 ACCAAAACCG GTTTCTCCCA CGAAGCGAGC GGTTTGTTCA CCGAAGTTGT GCGCATAACC
      721 GAAGAGTGCC GTCCGAAGTA CGTTTTTCTG GAAAACAGCC ACACCCTGAG CTTGTTCGAG
      781 AACATTTCCG TGATCGTGAA TGCCTTTGAT GAACTGGGTT ACGACTGTCG TTGGATTACG
      841 TGCAGAGCGA CGTGCGTGGG CGCACTGCAC CAGCGTCATC GTTGGTTTTG CCTGGTCGTC
      901 CGCCGTGATA TTGAGCTGGA CATCGGTATC CCGTATGTGG AGCGCTTTGA CTGGATGACC
      961 AACGAACCGC CTCGCCAGAT TCAAAAAAGC AATAAGGAAA ACATCCTGCA TATTAGCTTA
     1021 GCCGGCAACG CGGTTGTTCC GGATCAGGTT CGCTATGCTA TGGATATCCT TTGCTCCTTT
     1081 GACACCAACA AAGAGTTCTC CTTGTCGAGC CGTGCGAAAA ATGGCTTCAG TAAGAACGGC
     1141 GTTATTTTTA CCTATGACAT TGAGCACCCG GCGCGTGAGC CGCTGAATAT CGTGTTGACG
     1201 CCACGTGAGA ACGAGACCAG CTTCGTCATC TCTTGTGATC CGAAAAAGGT GCTCACGAAG
     1261 TCGGTCATCC AAAAGTTTTG GCCAACGCCG CTGCACAGCT GCAAATACGC TACCAAGTGC
     1321 CCGAGAACTC TTACCACCCG TGTGAGCAAG ATGCTGTCTG CGTGCGTTGG TTTCAGCGAA
     1381 GGTGGCGATA AAAACGGCTA CCTGTCCGCG AAATGGGTTC TGTGGCTGAT GGGTTACGAC
     1441 GACTCATACA TGAGCACCGC AGAAAGCAAG TATGAAATGA ATCGCGCCTT CTTTCTGGAC
     1501 ATCATCGTAG GTGAACGTGC GAGCGTGTTC GAATTCCAGC ATGATGAAGC AGTGGATAAC
     1561 AAGTTTAACA AAGAACAGCA GAACGCGTTT TATGAGATCT TGCACTTACC GAATCTGAAC
     1621 GAGGAACAGC GCAATGCGTT CATTCAGTCC CTTAAAGACG ATCCATCTCA AAGCGCAAAT
     1681 CTGCTGGCAG AAGCCAAGAA ACTGAACGAT GCTCAAGCTC CGAAAGTCGA CAACAAATTC
     1741 AACAAAGAGC AACAGAATGC GTTCTACGAA ATTCTGCATT TACCGAACCT GAATGAAGAA
     1801 CAGCGTAACG CCTTTATCCA ATCGCTGAAA GATGATCCTA GTCAGTCAGC CAATCTGTTG
     1861 GCGGAAGCGA AGAAACTCAA TGACGCCCAA GCTCCCAAAG TTGACGCGAA TAGCTGGAGC
     1921 CATCCGCAGT TTGAAAAGCT TTAGGGCTGT TTTGGCGGAT GAGAGAAGAT TTTCAGCCTG
     1981 ATACAGATTA AATCAGAACG CAGAAGCGGT CTGATAAAAC AGAATTTGCC TGGCGGCAGT
     2041 AGCGCGGTGG TCCCACCTGA CCCCATGCCG AACTCAGAAG TGAAACGCCG TAGCGCCGAT
     2101 GGTAGTGTGG GGTCTCCCCA TGCGAGAGTA GGGAACTGCC AGGCATCAAA TAAAACGAAA
     2161 GGCTCAGTCG AAAGACTGGG CCTTTCGTTT TATCTGTTGT TTGTCGGTGA ACGCTCTCCT
     2221 GAGTAGGACA AATCCGCCGG GAGCGGATTT GAACGTTGCG AAGCAACGGC CCGGAGGGTG
     2281 GCGGGCAGGA CGCCCGCCAT AAACTGCCAG GCATCAAATT AAGCAGAAGG CCATCCTGAC
     2341 GGATGGCCTT TTTGCGTTTC TACAAACTCT TTTTGTTTAT TTTTCTAAAT ACATTCAAAT
     2401 ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG
     2461 AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT
     2521 CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT
     2581 GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC
     2641 CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA
     2701 TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC
     2761 TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA
     2821 TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG
     2881 ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC
     2941 CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG
     3001 ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA
     3061 GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG
     3121 CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG
     3181 TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC
     3241 TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT
     3301 GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT
     3361 GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC
     3421 ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG
     3481 ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA
     3541 AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG
     3601 AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG
     3661 TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG
     3721 TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA
     3781 TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC
     3841 TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC
     3901 ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA
     3961 GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT
     4021 CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG
     4081 AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC
     4141 ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA
     4201 GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG
     4261 GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA
     4321 TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC
     4381 TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC
     4441 CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA
     4501 GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CAGATCAATT
     4561 CGCGCGCGAA GGCGAAGCGG CATGCATAAT GTGCCTGTCA AATGGACGAA GCAGGGATTC
     4621 TGCAAACCCT ATGCTACTCC GTCAAGCCGT CAATTGTCTG ATTCGTTACC AATTATGACA
     4681 ACTTGACGGC TACATCATTC ACTTTTTCTT CACAACCGGC ACGGAACTCG CTCGGGCTGG
     4741 CCCCGGTGCA TTTTTTAAAT ACCCGCGAGA AATAGAGTTG ATCGTCAAAA CCAACATTGC
     4801 GACCGACGGT GGCGATAGGC ATCCGGGTGG TGCTCAAAAG CAGCTTCGCC TGGCTGATAC
     4861 GTTGGTCCTC GCGCCAGCTT AAGACGCTAA TCCCTAACTG CTGGCGGAAA AAATGTGACA
     4921 GACGCGACGG CGACAAGCAA ACATGCTGTG CGACGCTGGC GATATCAAAA TTGCTGTCTG
     4981 CCAGGTGATC GCTGATGTAC TGACAAGCCT CGCGTACCCG ATTATCCATC GGTGGATGGA
     5041 GCGACTCGTT AATCGCTTCC ATGCGCCGCA GTAACAATTG CTCAAGCAGA TTTATCGCCA
     5101 GCAGCTCCGA ATAGCGCCCT TCCCCTTGCC CGGCGTTAAT GATTTGCCCA AACAGGTCGC
     5161 TGAAATGCGG CTGGTGCGCT TCATCCGGGC GAAAGAACCC CGTATTGGCA AATATTGACG
     5221 GCCAGTTAAG CCATTCATGC CAGTAGGCGC GCGGACGAAA GTAAACCCAC TGGTGATACC
     5281 ATTCGCGAGC CTCCGGATGA CGACCGTAGT GATGAATCTC TCCTGGCGGG AACAGCAAAA
     5341 TATCACCCGG TCGGCAAACA AATTCTCGTC CCTGATTTTT CACCACCCCC TGACCGCGAA
     5401 TGGTGAGATT GAGAATATAA CCTTTCATTC CCAGCGGTCG GTCGATAAAA AAATCGAGAT
     5461 AACCGTTGGC CTCAATCGGC GTTAAACCCG CCACCAGATG GGCATTAAAC GAGTATCCCG
     5521 GCAGCAGGGG ATCATTTTGC GCTTCAGCCA TACTTTTCAT ACTCCCGCCA TTCAGAG
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