LOCUS       pB-YP_009702000-ZZ-Strep      5523 bp    DNA     circular UNA 18-6-2020
DEFINITION  
FEATURES             Location/Qualifiers
     promoter        4..276
                     /label="araBAD"
     primer          9..33
                     /label="araBAD-F"
     primer          199..223
                     /label="araBAD-F2"
     primer          complement(294..318)
                     /label="araBAD-R"
     CDS             319..1890
                     /cds_type="ORF"
     Memo            331..348
                     /label="His6"
     PeptideTag      331..348
                     /label="His6"
     Memo            397..411
                     /label="DDDDK-tag"
     gene            424..1476
     gene            1483..1860
                     /label="ZZ"
     misc_feature    1861..1887
                     /feature_type="Protein Tag"
                     /label="Strep Tag"
     feature         1973..2130
                     /label="rrmB translation termination signal"
     primer          complement(1986..2010)
                     /label="araBAD-R2"
     gene            2410..3270
                     /label="AmpR"
     gene            4619..5487
                     /label="AraC Orf"
ORIGIN
        1 AAGAAACCAA TTGTCCATAT TGCATCAGAC ATTGCCGTCA CTGCGTCTTT TACTGGCTCT
       61 TCTCGCTAAC CAAACCGGTA ACCCCGCTTA TTAAAAGCAT TCTGTAACAA AGCGGGACCA
      121 AAGCCATGAC AAAAACGCGT AACAAAAGTG TCTATAATCA CGGCAGAAAA GTCCACATTG
      181 ATTATTTGCA CGGCGTCACA CTTTGCTATG CCATAGCATT TTTATCCATA AGATTAGCCG
      241 ATCCTACCTG ACGCTTTTTA TCGCAACTCT CTACTGTTTC TCCATACCCG TTTTTTGGGC
      301 TAACAGGAGG AATTAACCAT GGGGGGTTCT CATCATCATC ATCATCATGG TATGGCTAGC
      361 ATGACTGGTG GACAGCAAAT GGGTCGGGAT CTGTACGACG ATGACGATAA GCTCGAGGGA
      421 TCCATGCTGC GTGCGCTGGA CCTGTTCAGC GGCATCGGTG GCATTACCTA CGGCCTGCGT
      481 GGTATCGTTA CCCCGGTGGC GTATGTTGAG AAGAACAAAG ACGCGCGTGA ATTTCTGCAG
      541 AAGAAACACC CGGATGTTCC GGTGTTCGAC GATGTTTGCG CGTTTGACGC GACCGAGTGG
      601 AAGGGCAAAG TGGATATCAT TACCGGTGGC TGGCCGTGCA CCGGTTTCAG CATCGCGGGT
      661 AAAGGCGCGG GTTTCGAGCA CGAAGCGAGC GGTCTGTTTA GCGAGGTGGT TCGTATCACC
      721 AACGAGTGCG AACCGCGTTA CCTGTTCCTG GAAAACAGCC ACATTCTGAG CAAGAAAACC
      781 AACGTTAGCG TGGTTGTGAA CGCGCTGGAC AAGCTGGGCT ACGATTGCAA ATGGCTGACC
      841 TGCCGTGCGA CCAACGTGGG TGCGCCGCAC CAACGTCACC GTTGGTTCTG CCTGGTGATC
      901 AAGCGTGGCG CGAGCGTTAT TACCGACCTG CCGCTGGTTG ACACCTTTAA CTGGAGCAGC
      961 GGCGAGCCGG ACAAGCAGAT CAAAAACTAT ACCCCGATTA ACCGTACCGT TAGCAGCTGG
     1021 CTGGGTAACG CGGTTGTGCC GGACCAAGTG CGTTACGCGT TCACCACCCT GAACACCATG
     1081 AGCACCGAAA TCGCGACCTA TCAGCACGAT ATTAACAAGT TCCAAAACTG CGGTTTTAGC
     1141 CTGAAAAGCG AGATCTACTG GAGCCGTATC AAGATTCAGG AACGTCCGCC GCTGTATATT
     1201 ACCCTGACCC AAGAGAACCC GCCGAACAAG CACAAAGCGA CCACCCCGCA CATCAAAACC
     1261 ATTACCAAGA AATTCTGGAG CACCCCGGTT TTTAGCCACA ACCCGAGCGG CGTGAACTTC
     1321 CTGACCTACC GTAGCAGCAA GCTGCTGGGC ACCCAGGTTA AATTCGCGGA AGGTGGCTTT
     1381 GCGGGCATGT ATGTGAACCC GGATTGGGTT CGTTGGCTGA TGGGTTACCC GGAGGACTAT
     1441 TTTACCCTGG CGTACAAGGA TCACGAAACC CAAAGCGAAT TCCAGCATGA TGAAGCAGTG
     1501 GATAACAAGT TTAACAAAGA ACAGCAGAAC GCGTTTTATG AGATCTTGCA CTTACCGAAT
     1561 CTGAACGAGG AACAGCGCAA TGCGTTCATT CAGTCCCTTA AAGACGATCC ATCTCAAAGC
     1621 GCAAATCTGC TGGCAGAAGC CAAGAAACTG AACGATGCTC AAGCTCCGAA AGTCGACAAC
     1681 AAATTCAACA AAGAGCAACA GAATGCGTTC TACGAAATTC TGCATTTACC GAACCTGAAT
     1741 GAAGAACAGC GTAACGCCTT TATCCAATCG CTGAAAGATG ATCCTAGTCA GTCAGCCAAT
     1801 CTGTTGGCGG AAGCGAAGAA ACTCAATGAC GCCCAAGCTC CCAAAGTTGA CGCGAATAGC
     1861 TGGAGCCATC CGCAGTTTGA AAAGCTTTAG GGCTGTTTTG GCGGATGAGA GAAGATTTTC
     1921 AGCCTGATAC AGATTAAATC AGAACGCAGA AGCGGTCTGA TAAAACAGAA TTTGCCTGGC
     1981 GGCAGTAGCG CGGTGGTCCC ACCTGACCCC ATGCCGAACT CAGAAGTGAA ACGCCGTAGC
     2041 GCCGATGGTA GTGTGGGGTC TCCCCATGCG AGAGTAGGGA ACTGCCAGGC ATCAAATAAA
     2101 ACGAAAGGCT CAGTCGAAAG ACTGGGCCTT TCGTTTTATC TGTTGTTTGT CGGTGAACGC
     2161 TCTCCTGAGT AGGACAAATC CGCCGGGAGC GGATTTGAAC GTTGCGAAGC AACGGCCCGG
     2221 AGGGTGGCGG GCAGGACGCC CGCCATAAAC TGCCAGGCAT CAAATTAAGC AGAAGGCCAT
     2281 CCTGACGGAT GGCCTTTTTG CGTTTCTACA AACTCTTTTT GTTTATTTTT CTAAATACAT
     2341 TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA
     2401 AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT
     2461 TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG
     2521 TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT
     2581 TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG
     2641 GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG
     2701 AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA
     2761 AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG
     2821 ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA
     2881 ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC
     2941 ACCACGATGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT
     3001 ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA
     3061 CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG
     3121 CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA
     3181 GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG
     3241 ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT
     3301 TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT
     3361 AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA
     3421 GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA
     3481 ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT
     3541 TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG
     3601 CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA
     3661 ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA
     3721 AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG
     3781 CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA
     3841 AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA
     3901 ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC
     3961 GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC
     4021 CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT
     4081 GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT
     4141 GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG
     4201 GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC
     4261 CGCATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA
     4321 CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG
     4381 ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT
     4441 CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCAGA
     4501 TCAATTCGCG CGCGAAGGCG AAGCGGCATG CATAATGTGC CTGTCAAATG GACGAAGCAG
     4561 GGATTCTGCA AACCCTATGC TACTCCGTCA AGCCGTCAAT TGTCTGATTC GTTACCAATT
     4621 ATGACAACTT GACGGCTACA TCATTCACTT TTTCTTCACA ACCGGCACGG AACTCGCTCG
     4681 GGCTGGCCCC GGTGCATTTT TTAAATACCC GCGAGAAATA GAGTTGATCG TCAAAACCAA
     4741 CATTGCGACC GACGGTGGCG ATAGGCATCC GGGTGGTGCT CAAAAGCAGC TTCGCCTGGC
     4801 TGATACGTTG GTCCTCGCGC CAGCTTAAGA CGCTAATCCC TAACTGCTGG CGGAAAAAAT
     4861 GTGACAGACG CGACGGCGAC AAGCAAACAT GCTGTGCGAC GCTGGCGATA TCAAAATTGC
     4921 TGTCTGCCAG GTGATCGCTG ATGTACTGAC AAGCCTCGCG TACCCGATTA TCCATCGGTG
     4981 GATGGAGCGA CTCGTTAATC GCTTCCATGC GCCGCAGTAA CAATTGCTCA AGCAGATTTA
     5041 TCGCCAGCAG CTCCGAATAG CGCCCTTCCC CTTGCCCGGC GTTAATGATT TGCCCAAACA
     5101 GGTCGCTGAA ATGCGGCTGG TGCGCTTCAT CCGGGCGAAA GAACCCCGTA TTGGCAAATA
     5161 TTGACGGCCA GTTAAGCCAT TCATGCCAGT AGGCGCGCGG ACGAAAGTAA ACCCACTGGT
     5221 GATACCATTC GCGAGCCTCC GGATGACGAC CGTAGTGATG AATCTCTCCT GGCGGGAACA
     5281 GCAAAATATC ACCCGGTCGG CAAACAAATT CTCGTCCCTG ATTTTTCACC ACCCCCTGAC
     5341 CGCGAATGGT GAGATTGAGA ATATAACCTT TCATTCCCAG CGGTCGGTCG ATAAAAAAAT
     5401 CGAGATAACC GTTGGCCTCA ATCGGCGTTA AACCCGCCAC CAGATGGGCA TTAAACGAGT
     5461 ATCCCGGCAG CAGGGGATCA TTTTGCGCTT CAGCCATACT TTTCATACTC CCGCCATTCA
     5521 GAG
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