LOCUS       Copy_of_D666_pAAV-CKIIa 5681 bp ds-DNA     circular     30-DEC-2022
DEFINITION  .
KEYWORDS    "Gene" "Mutation" "Resistance" "Map" "Fluorophore" "name"
            "preperation" "concentration (ng/µl)"
FEATURES             Location/Qualifiers
     repeat_region   193..333
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     promoter        348..710
                     /label="a-CaMKII(0.4)"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    737..1798
                     /label="PdCO"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    1799..1825
                     /label="1d4"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             1850..1909
                     /label="ts"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     CDS             1913..2608
                     /label="mScarlet"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     CDS             2609..2629
                     /label="ER"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     misc_feature    2648..3051
                     /label="WPRE"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     polyA_signal    3058..3106
                     /label="poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     repeat_region   3136..3276
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     rep_origin      3351..3806
                     /label="f1 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     promoter        4088..4192
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             4193..4261
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             4262..5053
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     rep_origin      5224..131
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
ORIGIN
        1 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
       61 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
      121 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
      181 ttgctcacat gtcctgcagg cagctgcgcg ctcgctcgct cactgaggcc gcccgggcaa
      241 agcccgggcg tcgggcgacc tttggtcgcc cggcctcagt gagcgagcga gcgcgcagag
      301 agggagtggc caactccatc actaggggtt cctgcggccg cacgcgtctt gtggactaag
      361 tttgttcgca tccccttctc caaccccctc agtacatcac cctgggggaa cagggtccac
      421 ttgctcctgg gcccacacag tcctgcagta ttgtgtatat aaggccaggg caaagaggag
      481 caggttttaa agtgaaaggc aggcaggtgt tggggaggca gttaccgggg caacgggaac
      541 agggcgtttc ggaggtggtt gccatgggga cctggatgct gacgaaggct cgcgaggctg
      601 tgagcagcca cagtgccctg ctcagaagcc ccaagctcgt cagtcaagcc ggttctccgt
      661 ttgcactcag gagcacgggc aggcgagtgg cccctagttc tgggggcagc tctagagcgg
      721 taccgGATCC gccaccATGG ACGGGGAGAA CTTGACGATT CCAAATCCTG TCACCGAGTT
      781 GATGGATACC CCAATCAACA GCACGTATTT CCAAAACCTT AATGCGGAAA CGGACGGCGG
      841 AAATCATTAC ATCTACAATG CCTTCACAGC CACCGATTAC AACATTTGTG CAGCTTACCT
      901 TTTCTTCATT GCTTGTCTGG GAGTCAGTCT CAACGTTTTG GTGTTGGTCC TCTTTATCAA
      961 AGACAGAAAG TTGAGGAGCC CCAATAACTT CCTGTATGTA AGTCTCGCTC TCGGGGATCT
     1021 GTTGGTTGCA GTTTTCGGCA CCGCATTTAA GTTCATTATT ACAGCTCGCA AAACCCTGTT
     1081 GCGAGAAGAA GACGGTTTCT GCAAATGGTA CGGCTTCATA ACATACCTCG GCGGGCTCGC
     1141 GGCACTGATG ACCCTGAGCG TTATTGCTTT TGTTCGTTGT CTGGCCGTGC TGCGTCTCGG
     1201 CTCCTTCACA GGTTTGACAA CGCGGATGGG CGTGGCGGCG ATGGCTTTCA TTTGGATCTA
     1261 CTCCCTGGCA TTCACACTCG CGCCTCTCCT TGGGTGGAAT CACTACATAC CGGAAGGTTT
     1321 GGCCACTTGG TGTAGTATCG ACTGGCTTTC CGATGAAACA TCCGATAAGT CCTACGTGTT
     1381 TGCTATTTTC ATCTTCTGCT TTCTTGTCCC TGTTCTCATT ATCGTCGTGT CATACGGGCT
     1441 GATCTACGAT AAGGTTCGGA AGGTAGCGAA AACAGGCGGT TCTGTTGCCA AAGCAGAACG
     1501 CGAAGTACTG CGCATGACGC TGCTGATGGT TAGCCTGTTC ATGCTTGCCT GGAGTCCCTA
     1561 CGCTGTTATC TGCATGCTCG CTAGCTTCGG CCCTAAAGAC CTGCTCCATC CAGTGGCCAC
     1621 AGTGATTCCT GCCATGTTTG CAAAGTCTTC TACGATGTAT AACCCTCTTA TCTATGTGTT
     1681 CATGAACAAA CAATTCAGGC GGAGCCTTAA AGTTCTTCTC GGAATGGGAG TAGAGGACCT
     1741 GAACTCCGAG AGTGAGAGAG CAACTGGTGG TACAGCCACG AATCAAGTCG CCGCGACGAC
     1801 CGAGACTTCC CAGGTTGCTC CTGCTCCGCG aGCCCGaGAT CCAACCGGTA AGAGCAGGAT
     1861 CACCAGCGAG GGCGAGTACA TCCCCCTGGA CCAGATCGAC ATCAACGTGG TGatggtgag
     1921 caagggcgag gcagtgatca aggagttcat gcggttcaag gtgcacatgg agggctccat
     1981 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
     2041 gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttctcctggg acatcctgtc
     2101 ccctcagttc atgtacggct ccagggcctt caccaagcac cccgccgaca tccccgacta
     2161 ctataagcag tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
     2221 cggcgccgtg accgtgaccc aggacacctc cctggaggac ggcaccctga tctacaaggt
     2281 gaagctccgc ggcaccaact tccctcctga cggccccgta atgcagaaga agacaatggg
     2341 ctgggaagcg tccaccgagc ggttgtaccc cgaggacggc gtgctgaagg gcgacattaa
     2401 gatggccctg cgcctgaagg acggcggacg ctacctggcg gacttcaaga ccacctacaa
     2461 ggccaagaag cccgtgcaga tgcccggcgc ctacaacgtc gaccgcaagt tggacatcac
     2521 ctcccacaac gaggactaca ccgtggtgga acagtacgaa cgctccgagg gccgccactc
     2581 caccggcggc atggacgagc tgtacaagtT ctgctacgag aacgaggtgt AaCtaGAATT
     2641 CaagcttAAT CAACCTCTGG ATTACAAAAT TTGTGAAAGA TTGACTGATA TTCTTAACTA
     2701 TGTTGCTCCT TTTACGCTGT GTGGATATGC TGCTTTAATG CCTCTGTATC ATGCTATTGC
     2761 TTCCCGTATG GCTTTCGTTT TCTCCTCCTT GTATAAATCC TGGTTGCTGT CTCTTTATGA
     2821 GGAGTTGTGG CCCGTTGTCC GTCAACGTGG CGTGGTGTGC TCTGTGTTTG CTGACGCAAC
     2881 CCCCACTGGC TGGGGCATTG CCACCACCTG TCAACTCCTT TCTGGGACTT TCGCTTTCCC
     2941 CCTCCCGATC GCCACGGCAG AACTCATCGC CGCCTGCCTT GCCCGCTGCT GGACAGGGGC
     3001 TAGGTTGCTG GGCACTGATA ATTCCGTGGT GTTGTCGGGG AAGCTGACGT CagcgctAAT
     3061 AAAATCGCTA TCCATCGAAG ATGGATGTGT GTTGGTTTTT TGTGTGggta acccacgtgc
     3121 ggaccgagcg gccgcaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc
     3181 gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg
     3241 cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc
     3301 ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt acgcgccctg
     3361 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     3421 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     3481 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     3541 gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg
     3601 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     3661 ccaaactgga acaacactca accctatctc gggctattct tttgatttat aagggatttt
     3721 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     3781 taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct gctctgatgc
     3841 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     3901 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     3961 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
     4021 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     4081 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     4141 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     4201 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     4261 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     4321 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     4381 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     4441 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     4501 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     4561 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     4621 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     4681 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     4741 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     4801 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     4861 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     4921 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     4981 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     5041 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     5101 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     5161 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     5221 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     5281 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     5341 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     5401 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     5461 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     5521 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     5581 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     5641 agggagaaag gcggacaggt atccggtaag cggcagggtc g
//