LOCUS       Copy_of_D697_pAAV-hSyn- 6517 bp ds-DNA     circular     30-DEC-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      26..614
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     repeat_region   676..816
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     promoter        861..1308
                     /label="hSyn promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind    1322..1355
                     /label="lox71"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     misc_feature    complement(1368..1388)
                     /label="ER"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             complement(1389..2084)
                     /label="mScarlet"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     misc_feature    2085..2147
                     /label="TS"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(2172..2198)
                     /label="1d4"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    complement(2199..3260)
                     /label="PdCO"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     protein_bind    complement(3273..3306)
                     /label="lox66"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     misc_feature    3319..3907
                     /label="WPRE"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             complement(3790..3801)
                     /label="Factor Xa site"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     polyA_signal    3938..4418
                     /label="hGH polyA signal"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     repeat_region   4455..4595
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     rep_origin      4670..5125
                     /label="M13 origin"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     promoter        5407..5511
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             5512..5580
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             5581..6372
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
ORIGIN
        1 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
       61 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
      121 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
      181 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
      241 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
      301 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
      361 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
      421 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
      481 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
      541 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
      601 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
      661 cttttgctca catgtCctgc aggcagctgc gcgctcgctc gctcactgag gccgcccggg
      721 caaagcccgg gcgtcgggcg acctttggtc gcccggcctc agtgagcgag cgagcgcgca
      781 gagagggagt ggccaactcc atcactaggg gttcctgcgg ccgcacgcgt gtgtctagac
      841 tgcagagggc cctgcgtatg agtgcaagtg ggttttagga ccaggatgag gcggggtggg
      901 ggtgcctacc tgacgaccga ccccgaccca ctggacaagc acccaacccc cattccccaa
      961 attgcgcatc ccctatcaga gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca
     1021 ctgccagctt cagcaccgcg gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc
     1081 cgcctcagca ctgaaggcgc gctgacgtca ctcgccggtc ccccgcaaac tccccttccc
     1141 ggccaccttg gtcgcgtccg cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc
     1201 gagatagggg ggcacgggcg cgaccatctg cgctgcggcg ccggcgactc agcgctgcct
     1261 cagtctgcgg tgggcagcgg aggagtcgtg tcgtgcctga gagcgcagtc gagaaggtac
     1321 ctaccgttcg tatagcatac attatacgaa gttatgaatt CtaGtTacac ctcgttctcg
     1381 tagcagAact tgtacagctc gtccatgccg ccggtggagt ggcggccctc ggagcgttcg
     1441 tactgttcca ccacggtgta gtcctcgttg tgggaggtga tgtccaactt gcggtcgacg
     1501 ttgtaggcgc cgggcatctg cacgggcttc ttggccttgt aggtggtctt gaagtccgcc
     1561 aggtagcgtc cgccgtcctt caggcgcagg gccatcttaa tgtcgccctt cagcacgccg
     1621 tcctcggggt acaaccgctc ggtggacgct tcccagccca ttgtcttctt ctgcattacg
     1681 gggccgtcag gagggaagtt ggtgccgcgg agcttcacct tgtagatcag ggtgccgtcc
     1741 tccagggagg tgtcctgggt cacggtcacg gcgccgccgt cctcgaagtt catcacgcgc
     1801 tcccacttga agccctcggg gaaggactgc ttatagtagt cggggatgtc ggcggggtgc
     1861 ttggtgaagg ccctggagcc gtacatgaac tgaggggaca ggatgtccca ggagaagggc
     1921 agggggccac ccttggtcac cttcagcttg gcggtctggg tgccctcgta ggggcggccc
     1981 tcgccctcgc cctcgatctc gaactcgtgg ccgttcatgg agccctccat gtgcaccttg
     2041 aaccgcatga actccttgat cactgcctcg cccttgctca ccatCACCAC GTTGATGTCG
     2101 ATCTGGTCCA GGGGGATGTA CTCGCCCTCG CTGGTGATCC TGCTCTTACC GGTTGGATCt
     2161 CGGGCtCGCG GAGCAGGAGC AACCTGGGAA GTCTCGGTCG TCGCGGCGAC TTGATTCGTG
     2221 GCTGTACCAC CAGTTGCTCT CTCACTCTCG GAGTTCAGGT CCTCTACTCC CATTCCGAGA
     2281 AGAACTTTAA GGCTCCGCCT GAATTGTTTG TTCATGAACA CATAGATAAG AGGGTTATAC
     2341 ATCGTAGAAG ACTTTGCAAA CATGGCAGGA ATCACTGTGG CCACTGGATG GAGCAGGTCT
     2401 TTAGGGCCGA AGCTAGCGAG CATGCAGATA ACAGCGTAGG GACTCCAGGC AAGCATGAAC
     2461 AGGCTAACCA TCAGCAGCGT CATGCGCAGT ACTTCGCGTT CTGCTTTGGC AACAGAACCG
     2521 CCTGTTTTCG CTACCTTCCG AACCTTATCG TAGATCAGCC CGTATGACAC GACGATAATG
     2581 AGAACAGGGA CAAGAAAGCA GAAGATGAAA ATAGCAAACA CGTAGGACTT ATCGGATGTT
     2641 TCATCGGAAA GCCAGTCGAT ACTACACCAA GTGGCCAAAC CTTCCGGTAT GTAGTGATTC
     2701 CACCCAAGGA GAGGCGCGAG TGTGAATGCC AGGGAGTAGA TCCAAATGAA AGCCATCGCC
     2761 GCCACGCCCA TCCGCGTTGT CAAACCTGTG AAGGAGCCGA GACGCAGCAC GGCCAGACAA
     2821 CGAACAAAAG CAATAACGCT CAGGGTCATC AGTGCCGCGA GCCCGCCGAG GTATGTTATG
     2881 AAGCCGTACC ATTTGCAGAA ACCGTCTTCT TCTCGCAACA GGGTTTTGCG AGCTGTAATA
     2941 ATGAACTTAA ATGCGGTGCC GAAAACTGCA ACCAACAGAT CCCCGAGAGC GAGACTTACA
     3001 TACAGGAAGT TATTGGGGCT CCTCAACTTT CTGTCTTTGA TAAAGAGGAC CAACACCAAA
     3061 ACGTTGAGAC TGACTCCCAG ACAAGCAATG AAGAAAAGGT AAGCTGCACA AATGTTGTAA
     3121 TCGGTGGCTG TGAAGGCATT GTAGATGTAA TGATTTCCGC CGTCCGTTTC CGCATTAAGG
     3181 TTTTGGAAAT ACGTGCTGTT GATTGGGGTA TCCATCAACT CGGTGACAGG ATTTGGAATC
     3241 GTCAAGTTCT CCCCGTCCAT ggtggcGgat cctaccgttc gtataatgta tgctatacga
     3301 agttataagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     3361 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     3421 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     3481 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     3541 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     3601 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     3661 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     3721 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     3781 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     3841 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     3901 tccccgcatc gataccgagc gctgctcgag agatctacgg gtggcatccc tgtgacccct
     3961 ccccagtgcc tctcctggcc ctggaagttg ccactccagt gcccaccagc cttgtcctaa
     4021 taaaattaag ttgcatcatt ttgtctgact aggtgtcctt ctataatatt atggggtgga
     4081 ggggggtggt atggagcaag gggcaagttg ggaagacaac ctgtagggcc tgcggggtct
     4141 attgggaacc aagctggagt gcagtggcac aatcttggct cactgcaatc tccgcctcct
     4201 gggttcaagc gattctcctg cctcagcctc ccgagttgtt gggattccag gcatgcatga
     4261 ccaggctcag ctaatttttg tttttttggt agagacgggg tttcaccata ttggccaggc
     4321 tggtctccaa ctcctaatct caggtgatct acccaccttg gcctcccaaa ttgctgggat
     4381 tacaggcgtg aaccactgct cccttccctg tccttctgat tttgtaggta accacgtgcg
     4441 gaccgagcgg ccgcaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg
     4501 ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc
     4561 ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt attttctcct
     4621 tacgcatctg tgcggtattt cacaccgcat acgtcaaagc aaccatagta cgcgccctgt
     4681 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     4741 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     4801 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     4861 cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga
     4921 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     4981 caaactggaa caacactcaa ccctatctcg ggctattctt ttgatttata agggattttg
     5041 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     5101 aacaaaatat taacgtttac aattttatgg tgcactctca gtacaatctg ctctgatgcc
     5161 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     5221 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     5281 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
     5341 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     5401 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     5461 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     5521 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     5581 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     5641 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     5701 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     5761 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     5821 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     5881 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     5941 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     6001 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     6061 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     6121 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     6181 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     6241 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     6301 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     6361 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     6421 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     6481 ccttaacgtg agttttcgtt ccactgagcg tcagacc
//