LOCUS       Copy_of_D795_pAAV_hSyn- 5795 bp ds-DNA     circular     30-DEC-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     repeat_region   193..333
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     promoter        378..825
                     /label="hSyn promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    851..1912
                     /label="PdCO"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    1913..1939
                     /label="1d4"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             1964..2023
                     /label="ts"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     CDS             2027..2722
                     /label="mScarlet"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     CDS             2723..2743
                     /label="ER"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     misc_feature    2762..3165
                     /label="WPRE"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     polyA_signal    3172..3220
                     /label="poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     repeat_region   3250..3390
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     rep_origin      3465..3920
                     /label="f1 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     promoter        4202..4306
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             4307..4375
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             4376..5167
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     rep_origin      5338..131
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
ORIGIN
        1 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
       61 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
      121 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
      181 ttgctcacat gtcctgcagg cagctgcgcg ctcgctcgct cactgaggcc gcccgggcaa
      241 agcccgggcg tcgggcgacc tttggtcgcc cggcctcagt gagcgagcga gcgcgcagag
      301 agggagtggc caactccatc actaggggtt cctgcggccg cacgcgtgtg tctagactgc
      361 agagggccct gcgtatgagt gcaagtgggt tttaggacca ggatgaggcg gggtgggggt
      421 gcctacctga cgaccgaccc cgacccactg gacaagcacc caacccccat tccccaaatt
      481 gcgcatcccc tatcagagag ggggagggga aacaggatgc ggcgaggcgc gtgcgcactg
      541 ccagcttcag caccgcggac agtgccttcg cccccgcctg gcggcgcgcg ccaccgccgc
      601 ctcagcactg aaggcgcgct gacgtcactc gccggtcccc cgcaaactcc ccttcccggc
      661 caccttggtc gcgtccgcgc cgccgccggc ccagccggac cgcaccacgc gaggcgcgag
      721 ataggggggc acgggcgcga ccatctgcgc tgcggcgccg gcgactcagc gctgcctcag
      781 tctgcggtgg gcagcggagg agtcgtgtcg tgcctgagag cgcagtcgag aaggtaccgG
      841 ATCCgccacc ATGGACGGGG AGAACTTGAC GATTCCAAAT CCTGTCACCG AGTTGATGGA
      901 TACCCCAATC AACAGCACGT ATTTCCAAAA CCTTAATGCG GAAACGGACG GCGGAAATCA
      961 TTACATCTAC AATGCCTTCA CAGCCACCGA TTACAACATT TGTGCAGCTT ACCTTTTCTT
     1021 CATTGCTTGT CTGGGAGTCA GTCTCAACGT TTTGGTGTTG GTCCTCTTTA TCAAAGACAG
     1081 AAAGTTGAGG AGCCCCAATA ACTTCCTGTA TGTAAGTCTC GCTCTCGGGG ATCTGTTGGT
     1141 TGCAGTTTTC GGCACCGCAT TTAAGTTCAT TATTACAGCT CGCAAAACCC TGTTGCGAGA
     1201 AGAAGACGGT TTCTGCAAAT GGTACGGCTT CATAACATAC CTCGGCGGGC TCGCGGCACT
     1261 GATGACCCTG AGCGTTATTG CTTTTGTTCG TTGTCTGGCC GTGCTGCGTC TCGGCTCCTT
     1321 CACAGGTTTG ACAACGCGGA TGGGCGTGGC GGCGATGGCT TTCATTTGGA TCTACTCCCT
     1381 GGCATTCACA CTCGCGCCTC TCCTTGGGTG GAATCACTAC ATACCGGAAG GTTTGGCCAC
     1441 TTGGTGTAGT ATCGACTGGC TTTCCGATGA AACATCCGAT AAGTCCTACG TGTTTGCTAT
     1501 TTTCATCTTC TGCTTTCTTG TCCCTGTTCT CATTATCGTC GTGTCATACG GGCTGATCTA
     1561 CGATAAGGTT CGGAAGGTAG CGAAAACAGG CGGTTCTGTT GCCAAAGCAG AACGCGAAGT
     1621 ACTGCGCATG ACGCTGCTGA TGGTTAGCCT GTTCATGCTT GCCTGGAGTC CCTACGCTGT
     1681 TATCTGCATG CTCGCTAGCT TCGGCCCTAA AGACCTGCTC CATCCAGTGG CCACAGTGAT
     1741 TCCTGCCATG TTTGCAAAGT CTTCTACGAT GTATAACCCT CTTATCTATG TGTTCATGAA
     1801 CAAACAATTC AGGCGGAGCC TTAAAGTTCT TCTCGGAATG GGAGTAGAGG ACCTGAACTC
     1861 CGAGAGTGAG AGAGCAACTG GTGGTACAGC CACGAATCAA GTCGCCGCGA CGACCGAGAC
     1921 TTCCCAGGTT GCTCCTGCTC CGCGaGCCCG aGATCCAACC GGTAAGAGCA GGATCACCAG
     1981 CGAGGGCGAG TACATCCCCC TGGACCAGAT CGACATCAAC GTGGTGatgg tgagcaaggg
     2041 cgaggcagtg atcaaggagt tcatgcggtt caaggtgcac atggagggct ccatgaacgg
     2101 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
     2161 caagctgaag gtgaccaagg gtggccccct gcccttctcc tgggacatcc tgtcccctca
     2221 gttcatgtac ggctccaggg ccttcaccaa gcaccccgcc gacatccccg actactataa
     2281 gcagtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgc
     2341 cgtgaccgtg acccaggaca cctccctgga ggacggcacc ctgatctaca aggtgaagct
     2401 ccgcggcacc aacttccctc ctgacggccc cgtaatgcag aagaagacaa tgggctggga
     2461 agcgtccacc gagcggttgt accccgagga cggcgtgctg aagggcgaca ttaagatggc
     2521 cctgcgcctg aaggacggcg gacgctacct ggcggacttc aagaccacct acaaggccaa
     2581 gaagcccgtg cagatgcccg gcgcctacaa cgtcgaccgc aagttggaca tcacctccca
     2641 caacgaggac tacaccgtgg tggaacagta cgaacgctcc gagggccgcc actccaccgg
     2701 cggcatggac gagctgtaca agtTctgcta cgagaacgag gtgtAaCtaG AATTCaagct
     2761 tAATCAACCT CTGGATTACA AAATTTGTGA AAGATTGACT GATATTCTTA ACTATGTTGC
     2821 TCCTTTTACG CTGTGTGGAT ATGCTGCTTT AATGCCTCTG TATCATGCTA TTGCTTCCCG
     2881 TATGGCTTTC GTTTTCTCCT CCTTGTATAA ATCCTGGTTG CTGTCTCTTT ATGAGGAGTT
     2941 GTGGCCCGTT GTCCGTCAAC GTGGCGTGGT GTGCTCTGTG TTTGCTGACG CAACCCCCAC
     3001 TGGCTGGGGC ATTGCCACCA CCTGTCAACT CCTTTCTGGG ACTTTCGCTT TCCCCCTCCC
     3061 GATCGCCACG GCAGAACTCA TCGCCGCCTG CCTTGCCCGC TGCTGGACAG GGGCTAGGTT
     3121 GCTGGGCACT GATAATTCCG TGGTGTTGTC GGGGAAGCTG ACGTCagcgc tAATAAAATC
     3181 GCTATCCATC GAAGATGGAT GTGTGTTGGT TTTTTGTGTG ggtaacccac gtgcggaccg
     3241 agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc
     3301 tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag
     3361 tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc
     3421 atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg
     3481 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     3541 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     3601 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     3661 cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac
     3721 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     3781 tggaacaaca ctcaacccta tctcgggcta ttcttttgat ttataaggga ttttgccgat
     3841 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     3901 aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata
     3961 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     4021 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     4081 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
     4141 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     4201 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     4261 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     4321 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     4381 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     4441 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     4501 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     4561 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     4621 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     4681 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     4741 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     4801 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     4861 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     4921 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     4981 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     5041 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     5101 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     5161 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     5221 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     5281 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     5341 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     5401 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     5461 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     5521 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     5581 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     5641 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     5701 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     5761 aaaggcggac aggtatccgg taagcggcag ggtcg
//