LOCUS       pAAV_hSyn-DIO-PdCO-mSca 6458 bp ds-DNA     circular     27-APR-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     repeat_region   199..328
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     promoter        410..857
                     /label="hSyn promoter"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="human synapsin I promoter; confers neuron-specific expression (Kugler et al., 2003)"
     protein_bind    903..936
                     /label="lox2272"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="Cre-mediated recombination occurs in the 8-bp core sequence (GGATACTT). lox2272 sites are compatible with eachother, but incompatible with loxP or loxN sites (Lee and Saito, 1988)."
                     /bound_moiety="Cre recombinase"
     protein_bind    987..1020
                     /label="loxP"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)."
                     /bound_moiety="Cre recombinase"
     CDS             complement(1038..1058)
                     /label="ER"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     CDS             complement(1059..1754)
                     /label="mScarlet"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     CDS             complement(1758..1817)
                     /label="ts"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     misc_feature    complement(1842..1868)
                     /label="1d4"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    complement(1869..2930)
                     /label="PdCO"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     protein_bind    complement(2952..2985)
                     /label="lox2272"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="Cre-mediated recombination occurs in the 8-bp core sequence (GGATACTT). lox2272 sites are compatible with eachother, but incompatible with loxP or loxN sites (Lee and Saito, 1988)."
                     /bound_moiety="Cre recombinase"
     protein_bind    complement(3036..3069)
                     /label="loxP"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)."
                     /bound_moiety="Cre recombinase"
     misc_feature    3094..3682
                     /label="WPRE"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="woodchuck hepatitis virus posttranscriptional regulatory element"
     polyA_signal    3731..3852
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="SV40 polyadenylation signal"
     repeat_region   4014..4143
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             4145..4213
                     /label="LacZ alpha"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     rep_origin      4215..4670
                     /label="f1 ori"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
                     /direction="RIGHT"
     promoter        4696..4800
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /gene="bla"
     CDS             4999..5658
                     /label="AmpR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     rep_origin      5756..6438
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
ORIGIN
        1 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
       61 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
      121 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
      181 gccgattcat taatgcagct gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc
      241 gggcgtcggg cgacctttgg tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga
      301 gtggccaact ccatcactag gggttccttg tagttaatga ttaacccgcc atgctactta
      361 tctacgtagc catgctctag gaagatctct gcagagggcc ctgcgtatga gtgcaagtgg
      421 gttttaggac caggatgagg cggggtgggg gtgcctacct gacgaccgac cccgacccac
      481 tggacaagca cccaaccccc attccccaaa ttgcgcatcc cctatcagag agggggaggg
      541 gaaacaggat gcggcgaggc gcgtgcgcac tgccagcttc agcaccgcgg acagtgcctt
      601 cgcccccgcc tggcggcgcg cgccaccgcc gcctcagcac tgaaggcgcg ctgacgtcac
      661 tcgccggtcc cccgcaaact ccccttcccg gccaccttgg tcgcgtccgc gccgccgccg
      721 gcccagccgg accgcaccac gcgaggcgcg agataggggg gcacgggcgc gaccatctgc
      781 gctgcggcgc cggcgactca gcgctgcctc agtctgcggt gggcagcgga ggagtcgtgt
      841 cgtgcctgag agcgcagtcg aattcaagct gatagcaagg atcctctaga gtcgactccg
      901 gaataacttc gtataggata ctttatacga agttatgcag aatggtagct ggattgtagc
      961 tgctattagc aatatgaaac ctcttaataa cttcgtatag catacattat acgaagttat
     1021 ggcgcgccgt cgactTacac ctcgttctcg tagcagAact tgtacagctc gtccatgccg
     1081 ccggtggagt ggcggccctc ggagcgttcg tactgttcca ccacggtgta gtcctcgttg
     1141 tgggaggtga tgtccaactt gcggtcgacg ttgtaggcgc cgggcatctg cacgggcttc
     1201 ttggccttgt aggtggtctt gaagtccgcc aggtagcgtc cgccgtcctt caggcgcagg
     1261 gccatcttaa tgtcgccctt cagcacgccg tcctcggggt acaaccgctc ggtggacgct
     1321 tcccagccca ttgtcttctt ctgcattacg gggccgtcag gagggaagtt ggtgccgcgg
     1381 agcttcacct tgtagatcag ggtgccgtcc tccagggagg tgtcctgggt cacggtcacg
     1441 gcgccgccgt cctcgaagtt catcacgcgc tcccacttga agccctcggg gaaggactgc
     1501 ttatagtagt cggggatgtc ggcggggtgc ttggtgaagg ccctggagcc gtacatgaac
     1561 tgaggggaca ggatgtccca ggagaagggc agggggccac ccttggtcac cttcagcttg
     1621 gcggtctggg tgccctcgta ggggcggccc tcgccctcgc cctcgatctc gaactcgtgg
     1681 ccgttcatgg agccctccat gtgcaccttg aaccgcatga actccttgat cactgcctcg
     1741 cccttgctca ccatCACCAC GTTGATGTCG ATCTGGTCCA GGGGGATGTA CTCGCCCTCG
     1801 CTGGTGATCC TGCTCTTACC GGTTGGATCt CGGGCtCGCG GAGCAGGAGC AACCTGGGAA
     1861 GTCTCGGTCG TCGCGGCGAC TTGATTCGTG GCTGTACCAC CAGTTGCTCT CTCACTCTCG
     1921 GAGTTCAGGT CCTCTACTCC CATTCCGAGA AGAACTTTAA GGCTCCGCCT GAATTGTTTG
     1981 TTCATGAACA CATAGATAAG AGGGTTATAC ATCGTAGAAG ACTTTGCAAA CATGGCAGGA
     2041 ATCACTGTGG CCACTGGATG GAGCAGGTCT TTAGGGCCGA AGCTGGCGAG CATGCAGATA
     2101 ACAGCGTAGG GACTCCAGGC AAGCATGAAC AGGCTAACCA TCAGCAGCGT CATGCGCAGT
     2161 ACTTCGCGTT CTGCTTTGGC AACAGAACCG CCTGTTTTCG CTACCTTCCG AACCTTATCG
     2221 TAGATCAGCC CGTATGACAC GACGATAATG AGAACAGGGA CAAGAAAGCA GAAGATGAAA
     2281 ATAGCAAACA CGTAGGACTT ATCGGATGTT TCATCGGAAA GCCAGTCGAT ACTACACCAA
     2341 GTGGCCAAAC CTTCCGGTAT GTAGTGATTC CACCCAAGGA GAGGCGCGAG TGTGAATGCC
     2401 AGGGAGTAGA TCCAAATGAA AGCCATCGCC GCCACGCCCA TCCGCGTTGT CAAACCTGTG
     2461 AAGGAGCCGA GACGCAGCAC GGCCAGACAA CGAACAAAAG CAATAACGCT CAGGGTCATC
     2521 AGTGCCGCGA GCCCGCCGAG GTATGTTATG AAGCCGTACC ATTTGCAGAA ACCGTCTTCT
     2581 TCTCGCAACA GGGTTTTGCG AGCTGTAATA ATGAACTTAA ATGCGGTGCC GAAAACTGCA
     2641 ACCAACAGAT CCCCGAGAGC GAGACTTACA TACAGGAAGT TATTGGGGCT CCTCAACTTT
     2701 CTGTCTTTGA TAAAGAGGAC CAACACCAAA ACGTTGAGAC TGACTCCCAG ACAAGCAATG
     2761 AAGAAAAGGT AAGCTGCACA AATGTTGTAA TCGGTGGCTG TGAAGGCATT GTAGATGTAA
     2821 TGATTTCCGC CGTCCGTTTC CGCATTAAGG TTTTGGAAAT ACGTGCTGTT GATTGGGGTA
     2881 TCCATCAACT CGGTGACAGG ATTTGGAATC GTCAAGTTCT CCCCGTCCAT ggtggcaaca
     2941 gcgctgctag cataacttcg tataaagtat cctatacgaa gttatttgcc ttaacccaga
     3001 aattatcact gttattcttt agaatggtgc aaagaataac ttcgtataat gtatgctata
     3061 cgaagttatg aattcgatat caagcttatc gataatcaac ctctggatta caaaatttgt
     3121 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     3181 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     3241 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     3301 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     3361 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     3421 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     3481 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     3541 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     3601 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     3661 tccctttggg ccgcctcccc gcatcgatac cgtcgacctc gacccgggcg gccgcttcga
     3721 gcagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
     3781 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     3841 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggagatg
     3901 tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcga taaggatctt
     3961 cctagagcat ggctacgtag ataagtagca tggcgggtta atcattaact acaaggaacc
     4021 cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg
     4081 accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg
     4141 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     4201 gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     4261 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     4321 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     4381 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     4441 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     4501 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     4561 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     4621 ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact
     4681 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     4741 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     4801 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     4861 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     4921 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     4981 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     5041 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     5101 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     5161 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     5221 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     5281 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     5341 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     5401 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     5461 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     5521 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     5581 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     5641 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     5701 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     5761 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     5821 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     5881 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     5941 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     6001 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     6061 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     6121 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     6181 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     6241 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     6301 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     6361 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     6421 aacgccagca acgcggcctt tttacggttc ctggcctt
//