LOCUS       Copy_of_D800_pAAV_hSyn- 5816 bp ds-DNA     circular     30-DEC-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     repeat_region   193..333
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     promoter        378..825
                     /label="hSyn promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    851..1912
                     /label="PdCO"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             851..2769
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MDGENLTIPNPVTELMDTPINSTYFQNLNAETDGGNHYIYNAFTATDYNICAAYLFFIACLGVSLNVLVLVLFIKDRKLRSPNNFLYVSLALGDLLVAVFGTAFKFIITARKTLLREEDGFCKWYGFITYLGGLAALMTLSVIAFVRCLAVLRLGSFTGLTTRMGVAAMAFIWIYSLAFTLAPLLGWNHYIPEGLATWCSIDWLSDETSDKSYVFAIFIFCFLVPVLIIVVSYGLIYDKVRKVAKTGGSVAKAEREVLRMTLLMVSLFMLAWSPYAVICMLASFGPKDLLHPVATVIPAMFAKSSTMYNPLIYVFMNKQFRRSLKVLLGMGVEDLNSESERATGGTATNQVAATTETSQVAPAPRARDPTGKSRITSEGEYIPLDQIDINVVMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKFCYENEV*"
     misc_feature    1913..1939
                     /label="1d4"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             1964..2023
                     /label="ts"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     CDS             2027..2743
                     /label="EGFP"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="product: enhanced GFP translation: VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(2333..2352)
                     /label="EXFP-R"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: For distinguishing EGFP variants, reverse primer"
     CDS             2744..2764
                     /label="ER"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     misc_feature    2783..3186
                     /label="WPRE"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     polyA_signal    3193..3241
                     /label="poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     repeat_region   3271..3411
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     rep_origin      3486..3941
                     /label="f1 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     promoter        4223..4327
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             4328..4396
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             4397..5188
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     rep_origin      5359..131
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
ORIGIN
        1 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
       61 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
      121 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
      181 ttgctcacat gtcctgcagg cagctgcgcg ctcgctcgct cactgaggcc gcccgggcaa
      241 agcccgggcg tcgggcgacc tttggtcgcc cggcctcagt gagcgagcga gcgcgcagag
      301 agggagtggc caactccatc actaggggtt cctgcggccg cacgcgtgtg tctagactgc
      361 agagggccct gcgtatgagt gcaagtgggt tttaggacca ggatgaggcg gggtgggggt
      421 gcctacctga cgaccgaccc cgacccactg gacaagcacc caacccccat tccccaaatt
      481 gcgcatcccc tatcagagag ggggagggga aacaggatgc ggcgaggcgc gtgcgcactg
      541 ccagcttcag caccgcggac agtgccttcg cccccgcctg gcggcgcgcg ccaccgccgc
      601 ctcagcactg aaggcgcgct gacgtcactc gccggtcccc cgcaaactcc ccttcccggc
      661 caccttggtc gcgtccgcgc cgccgccggc ccagccggac cgcaccacgc gaggcgcgag
      721 ataggggggc acgggcgcga ccatctgcgc tgcggcgccg gcgactcagc gctgcctcag
      781 tctgcggtgg gcagcggagg agtcgtgtcg tgcctgagag cgcagtcgag aaggtaccgG
      841 ATCCgccacc ATGGACGGGG AGAACTTGAC GATTCCAAAT CCTGTCACCG AGTTGATGGA
      901 TACCCCAATC AACAGCACGT ATTTCCAAAA CCTTAATGCG GAAACGGACG GCGGAAATCA
      961 TTACATCTAC AATGCCTTCA CAGCCACCGA TTACAACATT TGTGCAGCTT ACCTTTTCTT
     1021 CATTGCTTGT CTGGGAGTCA GTCTCAACGT TTTGGTGTTG GTCCTCTTTA TCAAAGACAG
     1081 AAAGTTGAGG AGCCCCAATA ACTTCCTGTA TGTAAGTCTC GCTCTCGGGG ATCTGTTGGT
     1141 TGCAGTTTTC GGCACCGCAT TTAAGTTCAT TATTACAGCT CGCAAAACCC TGTTGCGAGA
     1201 AGAAGACGGT TTCTGCAAAT GGTACGGCTT CATAACATAC CTCGGCGGGC TCGCGGCACT
     1261 GATGACCCTG AGCGTTATTG CTTTTGTTCG TTGTCTGGCC GTGCTGCGTC TCGGCTCCTT
     1321 CACAGGTTTG ACAACGCGGA TGGGCGTGGC GGCGATGGCT TTCATTTGGA TCTACTCCCT
     1381 GGCATTCACA CTCGCGCCTC TCCTTGGGTG GAATCACTAC ATACCGGAAG GTTTGGCCAC
     1441 TTGGTGTAGT ATCGACTGGC TTTCCGATGA AACATCCGAT AAGTCCTACG TGTTTGCTAT
     1501 TTTCATCTTC TGCTTTCTTG TCCCTGTTCT CATTATCGTC GTGTCATACG GGCTGATCTA
     1561 CGATAAGGTT CGGAAGGTAG CGAAAACAGG CGGTTCTGTT GCCAAAGCAG AACGCGAAGT
     1621 ACTGCGCATG ACGCTGCTGA TGGTTAGCCT GTTCATGCTT GCCTGGAGTC CCTACGCTGT
     1681 TATCTGCATG CTCGCTAGCT TCGGCCCTAA AGACCTGCTC CATCCAGTGG CCACAGTGAT
     1741 TCCTGCCATG TTTGCAAAGT CTTCTACGAT GTATAACCCT CTTATCTATG TGTTCATGAA
     1801 CAAACAATTC AGGCGGAGCC TTAAAGTTCT TCTCGGAATG GGAGTAGAGG ACCTGAACTC
     1861 CGAGAGTGAG AGAGCAACTG GTGGTACAGC CACGAATCAA GTCGCCGCGA CGACCGAGAC
     1921 TTCCCAGGTT GCTCCTGCTC CGCGaGCCCG aGATCCAACC GGTAAGAGCA GGATCACCAG
     1981 CGAGGGCGAG TACATCCCCC TGGACCAGAT CGACATCAAC GTGGTGatgg tgagcaaggg
     2041 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     2101 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     2161 gaagttcatt tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
     2221 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
     2281 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     2341 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     2401 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     2461 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
     2521 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     2581 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
     2641 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     2701 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtTctgct acgagaacga
     2761 ggtgtAaCta GAATTCaagc ttAATCAACC TCTGGATTAC AAAATTTGTG AAAGATTGAC
     2821 TGATATTCTT AACTATGTTG CTCCTTTTAC GCTGTGTGGA TATGCTGCTT TAATGCCTCT
     2881 GTATCATGCT ATTGCTTCCC GTATGGCTTT CGTTTTCTCC TCCTTGTATA AATCCTGGTT
     2941 GCTGTCTCTT TATGAGGAGT TGTGGCCCGT TGTCCGTCAA CGTGGCGTGG TGTGCTCTGT
     3001 GTTTGCTGAC GCAACCCCCA CTGGCTGGGG CATTGCCACC ACCTGTCAAC TCCTTTCTGG
     3061 GACTTTCGCT TTCCCCCTCC CGATCGCCAC GGCAGAACTC ATCGCCGCCT GCCTTGCCCG
     3121 CTGCTGGACA GGGGCTAGGT TGCTGGGCAC TGATAATTCC GTGGTGTTGT CGGGGAAGCT
     3181 GACGTCagcg ctAATAAAAT CGCTATCCAT CGAAGATGGA TGTGTGTTGG TTTTTTGTGT
     3241 Gggtaaccca cgtgcggacc gagcggccgc aggaacccct agtgatggag ttggccactc
     3301 cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg
     3361 gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga
     3421 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc
     3481 atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     3541 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     3601 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     3661 atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag
     3721 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     3781 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcgggct attcttttga
     3841 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     3901 atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac
     3961 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
     4021 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     4081 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct
     4141 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     4201 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     4261 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     4321 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     4381 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     4441 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     4501 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     4561 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     4621 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     4681 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     4741 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     4801 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     4861 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     4921 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     4981 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     5041 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     5101 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     5161 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     5221 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     5281 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     5341 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     5401 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     5461 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     5521 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     5581 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     5641 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     5701 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     5761 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcg
//