LOCUS       pAAV_EF1a-fDIO-PdCO-mSc 6819 bp ds-DNA     circular     08-MAY-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     repeat_region   609..738
                     /label="AAV2 ITR (alternate)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="note: inverted terminal repeat of adeno-associated virus serotype 2 rpt_type: inverted: "
     promoter        753..2017
                     /label="EF-1a"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind    2048..2081
                     /label="FRT (minimal)"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="note: supports FLP-mediated excision but not integration (Turan and Bode, 2011) bound_moiety: FLP recombinase from the Saccharomyces cerevisiae 2μ plasmid"
     protein_bind    2132..2165
                     /label="F5"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="note: Flp recombinase site 5 bound_moiety: Flp recombinase"
     CDS             complement(2183..2203)
                     /label="ER"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     CDS             complement(2204..2899)
                     /label="mScarlet"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     CDS             complement(2903..2962)
                     /label="ts"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     misc_feature_   complement(2968..2986)
                     /label="3'"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    complement(2987..3013)
                     /label="1d4"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    complement(3014..4075)
                     /label="PdCO"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     protein_bind    complement(4097..4130)
                     /label="FRT (minimal)"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="note: supports FLP-mediated excision but not integration (Turan and Bode, 2011) bound_moiety: FLP recombinase from the Saccharomyces cerevisiae 2μ plasmid"
     protein_bind    complement(4181..4214)
                     /label="F5"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="note: Flp recombinase site 5 bound_moiety: Flp recombinase"
     misc_feature    4239..4668
                     /label="minWPRE"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     repeat_region   4690..4830
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="note: inverted terminal repeat of adeno-associated virus serotype 2 rpt_type: inverted: "
     rep_origin      4905..5360
                     /label="f1 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="note: f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis direction: RIGHT"
     promoter        5642..5746
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="gene: bla"
     CDS             5747..5815
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="note: confers resistance to ampicillin, carbenicillin, and related antibiotics transl_table: 1 product: β-lactamase codon_start: 1 translation: MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW* gene: bla"
     CDS             5816..6607
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="note: confers resistance to ampicillin, carbenicillin, and related antibiotics transl_table: 1 product: β-lactamase codon_start: 1 translation: MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW* gene: bla"
     rep_origin      6755..603
                     /label="pUC ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
ORIGIN
        1 AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT
       61 TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTTCTTC TAGTGTAGCC
      121 GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT
      181 CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG
      241 ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC
      301 CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG
      361 CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC
      421 AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG
      481 GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT
      541 ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC
      601 TCACATGTCC TGCAGGCAGC TGCGCGCTCG CTCGCTCACT GAGGCCGCCC GGGCGTCGGG
      661 CGACCTTTGG TCGCCCGGCC TCAGTGAGCG AGCGAGCGCG CAGAGAGGGA GTGGCCAACT
      721 CCATCACTAG GGGTTCCTGC GGCCGCACGC GTAAGCTTTG CAAAGATGGA TAAAGTTTTA
      781 AACAGAGAGG AATCTTTGCA GCTAATGGAC CTTCTAGGTC TTGAAAGGAG TGGGAATTGG
      841 CTCCGGTGCC CGTCAGTGGG CAGAGCGCAC ATCGCCCACA GTCCCCGAGA AGTTGGGGGG
      901 AGGGGTCGGC AATTGAACCG GTGCCTAGAG AAGGTGGCGC GGGGTAAACT GGGAAAGTGA
      961 TGTCGTGTAC TGGCTCCGCC TTTTTCCCGA GGGTGGGGGA GAACCGTATA TAAGTGCAGT
     1021 AGTCGCCGTG AACGTTCTTT TTCGCAACGG GTTTGCCGCC AGAACACAGG TAAGTGCCGT
     1081 GTGTGGTTCC CGCGGGCCTG GCCTCTTTAC GGGTTATGGC CCTTGCGTGC CTTGAATTAC
     1141 TTCCACCTGG CTGCAGTACG TGATTCTTGA TCCCGAGCTT CGGGTTGGAA GTGGGTGGGA
     1201 GAGTTCGAGG CCTTGCGCTT AAGGAGCCCC TTCGCCTCGT GCTTGAGTTG AGGCCTGGCC
     1261 TGGGCGCTGG GGCCGCCGCG TGCGAATCTG GTGGCACCTT CGCGCCTGTC TCGCTGCTTT
     1321 CGATAAGTCT CTAGCCATTT AAAATTTTTG ATGACCTGCT GCGACGCTTT TTTTCTGGCA
     1381 AGATAGTCTT GTAAATGCGG GCCAAGATCT GCACACTGGT ATTTCGGTTT TTGGGGCCGC
     1441 GGGCGGCGAC GGGGCCCGTG CGTCCCAGCG CACATGTTCG GCGAGGCGGG GCCTGCGAGC
     1501 GCGGCCACCG AGAATCGGAC GGGGGTAGTC TCAAGCTGGC CGGCCTGCTC TGGTGCCTGG
     1561 CCTCGCGCCG CCGTGTATCG CCCCGCCCTG GGCGGCAAGG CTGGCCCGGT CGGCACCAGT
     1621 TGCGTGAGCG GAAAGATGGC CGCTTCCCGG CCCTGCTGCA GGGAGCTCAA AATGGAGGAC
     1681 GCGGCGCTCG GGAGAGCGGG CGGGTGAGTC ACCCACACAA AGGAAAAGGG CCTTTCCGTC
     1741 CTCAGCCGTC GCTTCATGTG ACTCCACGGA GTACCGGGCG CCGTCCAGGC ACCTCGATTA
     1801 GTTCTCGAGC TTTTGGAGTA CGTCGTCTTT AGGTTGGGGG GAGGGGTTTT ATGCGATGGA
     1861 GTTTCCCCAC ACTGAGTGGG TGGAGACTGA AGTTAGGCCA GCTTGGCACT TGATGTAATT
     1921 CTCCTTGGAA TTTGCCCTTT TTGAGTTTGG ATCTTGGTTC ATTCTCAAGC CTCAGACAGT
     1981 GGTTCAAAGT TTTTTTCTTC CATTTCAGGT GTCGTGAGGT ACCGGATCCT CTAGAGTCGA
     2041 CTCCGGAGAA GTTCCTATTC TCTAGAAAGT ATAGGAACTT CGCAGAATGG TAGCTGGATT
     2101 GTAGCTGCTA TTAGCAATAT GAAACCTCTT AGAAGTTCCT ATTCTTCAAA AGGTATAGGA
     2161 ACTTCGGcgc gccgtcgact Tacacctcgt tctcgtagca gAacttgtac agctcgtcca
     2221 tgccgccggt ggagtggcgg ccctcggagc gttcgtactg ttccaccacg gtgtagtcct
     2281 cgttgtggga ggtgatgtcc aacttgcggt cgacgttgta ggcgccgggc atctgcacgg
     2341 gcttcttggc cttgtaggtg gtcttgaagt ccgccaggta gcgtccgccg tccttcaggc
     2401 gcagggccat cttaatgtcg cccttcagca cgccgtcctc ggggtacaac cgctcggtgg
     2461 acgcttccca gcccattgtc ttcttctgca ttacggggcc gtcaggaggg aagttggtgc
     2521 cgcggagctt caccttgtag atcagggtgc cgtcctccag ggaggtgtcc tgggtcacgg
     2581 tcacggcgcc gccgtcctcg aagttcatca cgcgctccca cttgaagccc tcggggaagg
     2641 actgcttata gtagtcgggg atgtcggcgg ggtgcttggt gaaggccctg gagccgtaca
     2701 tgaactgagg ggacaggatg tcccaggaga agggcagggg gccacccttg gtcaccttca
     2761 gcttggcggt ctgggtgccc tcgtaggggc ggccctcgcc ctcgccctcg atctcgaact
     2821 cgtggccgtt catggagccc tccatgtgca ccttgaaccg catgaactcc ttgatcactg
     2881 cctcgccctt gctcaccatC ACCACGTTGA TGTCGATCTG GTCCAGGGGG ATGTACTCGC
     2941 CCTCGCTGGT GATCCTGCTC TTACCGGTTG GATCtCGGGC tCGCGGAGCA GGAGCAACCT
     3001 GGGAAGTCTC GGTCGTCGCG GCGACTTGAT TCGTGGCTGT ACCACCAGTT GCTCTCTCAC
     3061 TCTCGGAGTT CAGGTCCTCT ACTCCCATTC CGAGAAGAAC TTTAAGGCTC CGCCTGAATT
     3121 GTTTGTTCAT GAACACATAG ATAAGAGGGT TATACATCGT AGAAGACTTT GCAAACATGG
     3181 CAGGAATCAC TGTGGCCACT GGATGGAGCA GGTCTTTAGG GCCGAAGCTG GCGAGCATGC
     3241 AGATAACAGC GTAGGGACTC CAGGCAAGCA TGAACAGGCT AACCATCAGC AGCGTCATGC
     3301 GCAGTACTTC GCGTTCTGCT TTGGCAACAG AACCGCCTGT TTTCGCTACC TTCCGAACCT
     3361 TATCGTAGAT CAGCCCGTAT GACACGACGA TAATGAGAAC AGGGACAAGA AAGCAGAAGA
     3421 TGAAAATAGC AAACACGTAG GACTTATCGG ATGTTTCATC GGAAAGCCAG TCGATACTAC
     3481 ACCAAGTGGC CAAACCTTCC GGTATGTAGT GATTCCACCC AAGGAGAGGC GCGAGTGTGA
     3541 ATGCCAGGGA GTAGATCCAA ATGAAAGCCA TCGCCGCCAC GCCCATCCGC GTTGTCAAAC
     3601 CTGTGAAGGA GCCGAGACGC AGCACGGCCA GACAACGAAC AAAAGCAATA ACGCTCAGGG
     3661 TCATCAGTGC CGCGAGCCCG CCGAGGTATG TTATGAAGCC GTACCATTTG CAGAAACCGT
     3721 CTTCTTCTCG CAACAGGGTT TTGCGAGCTG TAATAATGAA CTTAAATGCG GTGCCGAAAA
     3781 CTGCAACCAA CAGATCCCCG AGAGCGAGAC TTACATACAG GAAGTTATTG GGGCTCCTCA
     3841 ACTTTCTGTC TTTGATAAAG AGGACCAACA CCAAAACGTT GAGACTGACT CCCAGACAAG
     3901 CAATGAAGAA AAGGTAAGCT GCACAAATGT TGTAATCGGT GGCTGTGAAG GCATTGTAGA
     3961 TGTAATGATT TCCGCCGTCC GTTTCCGCAT TAAGGTTTTG GAAATACGTG CTGTTGATTG
     4021 GGGTATCCAT CAACTCGGTG ACAGGATTTG GAATCGTCAA GTTCTCCCCG TCCATggtgg
     4081 caacagcgct gCTAGCGAAG TTCCTATACT TTCTAGAGAA TAGGAACTTC TTGCCTTAAC
     4141 CCAGAAATTA TCACTGTTAT TCTTTAGAAT GGTGCAAAGA GAAGTTCCTA TACCTTTTGA
     4201 AGAATAGGAA CTTCGAATTC GATATCAAGC TTATCGATga taatcaacct ctggattaca
     4261 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     4321 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     4381 ccttgtataa atcctggtta gttcttgcca cggcggaact catcgccgcc tgccttgccc
     4441 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttt atttgtgaaa
     4501 tttgtgatgc tattgcttta tttgtaacca tctagcttta tttgtgaaat ttgtgatgct
     4561 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
     4621 cattttatgt ttcaggttca gggggagatg tgggaggttt tttaaagcac GTGCGGACCG
     4681 AGCGGCCGCA GGAACCCCTA GTGATGGAGT TGGCCACTCC CTCTCTGCGC GCTCGCTCGC
     4741 TCACTGAGGC CGGGCGACCA AAGGTCGCCC GACGCCCGGG CTTTGCCCGG GCGGCCTCAG
     4801 TGAGCGAGCG AGCGCGCAGC TGCCTGCAGG GGCGCCTGAT GCGGTATTTT CTCCTTACGC
     4861 ATCTGTGCGG TATTTCACAC CGCATACGTC AAAGCAACCA TAGTACGCGC CCTGTAGCGG
     4921 CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC
     4981 CTTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC
     5041 CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT
     5101 CGACCCCAAA AAACTTGATT TGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC
     5161 GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC
     5221 TGGAACAACA CTCAACTCTA TCTCGGGCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT
     5281 TTCGGTCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTTAACAA
     5341 AATATTAACG TTTACAATTT TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA
     5401 GTTAAGCCAG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT
     5461 CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT
     5521 TTCACCGTCA TCACCGAAAC GCGCGAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA
     5581 GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT
     5641 GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG
     5701 ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA
     5761 TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC
     5821 AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT
     5881 CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC
     5941 AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTA TTGACGCCGG
     6001 GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC
     6061 AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT
     6121 AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA
     6181 GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC
     6241 GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC
     6301 AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT
     6361 AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC
     6421 TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC
     6481 AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA
     6541 GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA
     6601 TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT
     6661 TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA
     6721 ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG
     6781 AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAAC
//