LOCUS       Exported                5684 bp ds-DNA     circular SYN 16-FEB-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Feb 16, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5684
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        27..311
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     CDS             357..374
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             378..410
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             414..437
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     terminator      2281..2367
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2459..2486
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        2505..2596
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2597..3457
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3628..4216
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    4402..4542
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(4806..5684)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 acttttcata ctcccgccat tcagagaaga aaccaattgt ccatattgca tcagacattg
       61 ccgtcactgc gtcttttact ggctcttctc gctaaccaaa ccggtaaccc cgcttattaa
      121 aagcattctg taacaaagcg ggaccaaagc catgacaaaa acgcgtaaca aaagtgtcta
      181 taatcacggc agaaaagtcc acattgatta tttgcacggc gtcacacttt gctatgccat
      241 agcattttta tccataagat tagcggatcc tacctgacgc tttttatcgc aactctctac
      301 tgtttctcca tacccgtttt ttgggctaac aggaggaatt aaccatgggg ggttctcatc
      361 atcatcatca tcatggtatg gctagcatga ctggtggaca gcaaatgggt cgggatctgt
      421 acgacgatga cgataaggat ccgagctcga gaatggcaga aatcggtact ggctttccat
      481 tcgaccccca ttatgtggaa gtcctgggcg agcgcatgca ctacgtcgat gttggtccgc
      541 gcgatggcac ccctgtgctg ttcctgcacg gtaacccgac ctcctcctac gtgtggcgca
      601 acatcatccc gcatgttgca ccgacccatc gctgcattgc tccagacctg atcggtatgg
      661 gcaaatccga caaaccagac ctgggttatt tcttcgacga ccacgtccgc ttcatggatg
      721 ccttcatcga agccctgggt ctggaagagg tcgtcctggt cattcacgac tggggctccg
      781 ctctgggttt ccactgggcc aagcgcaatc cagagcgcgt caaaggtatt gcatttatgg
      841 agttcatccg ccctatcccg acctgggacg aatggccaga atttgcccgc gagaccttcc
      901 aggccttcgg tgaaaacgtc tatatcaagg ccgacaagca gaagaacggc atcaaggcga
      961 acttcaagat ccgccacaac atcgaggacg gcggcgtgca gctcgcctac cactaccagc
     1021 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcgtcc
     1081 agtccaaact ttcgaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     1141 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagggcggt accggaggga
     1201 gcatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     1261 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggtgagggc gatgccacct
     1321 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     1381 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
     1441 agcagcacga cttcttcaag tccgccatgc ccgaaggcta catccaggag cgcaccatct
     1501 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     1561 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     1621 ataagctgga gtacaacctt ggcgacgtcg gccgcaagct gatcatcgat cagaacgttt
     1681 ttatcgaggg tacgctgccg atgggtgtcg tccgcccgct gactgaagtc gagatggacc
     1741 attaccgcga gccgttcctg aatcctgttg accgcgagcc actgtggcgc ttcccaaacg
     1801 agctgccaat cgccggtgag ccagcgaaca tcgtcgcgct ggtcgaagaa tacatggact
     1861 ggctgcacca gtcccctgtc ccgaagctgc tgttctgggg caccccaggc gttctgatcc
     1921 caccggccga agccgctcgc ctggccaaaa gcctgcctaa ctgcaaggct gtggacatcg
     1981 gcccgggtct gaatctgctg caagaagaca acccggacct gatcggcagc gagatcgcgc
     2041 gctggctgtc gacgctcgag atttccggct gaaagcttgg ctgttttggc ggatgagaga
     2101 agattttcag cctgatacag attaaatcag aacgcagaag cggtctgata aaacagaatt
     2161 tgcctggcgg cagtagcgcg gtggtcccac ctgaccccat gccgaactca gaagtgaaac
     2221 gccgtagcgc cgatggtagt gtggggtctc cccatgcgag agtagggaac tgccaggcat
     2281 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
     2341 gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg atttgaacgt tgcgaagcaa
     2401 cggcccggag ggtggcgggc aggacgcccg ccataaactg ccaggcatca aattaagcag
     2461 aaggccatcc tgacggatgg cctttttgcg tttctacaaa ctcttttgtt tatttttcta
     2521 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     2581 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     2641 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     2701 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     2761 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     2821 tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     2881 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     2941 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     3001 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     3061 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     3121 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     3181 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     3241 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     3301 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     3361 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     3421 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     3481 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     3541 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     3601 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     3661 cttgcaaaca aaacaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     3721 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     3781 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     3841 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     3901 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     3961 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     4021 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     4081 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     4141 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     4201 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     4261 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     4321 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     4381 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     4441 ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     4501 gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca
     4561 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     4621 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg
     4681 cagcagatca attcgcgcgc gaaggcgaag cggcatgcat aatgtgcctg tcaaatggac
     4741 gaagcaggga ttctgcaaac cctatgctac tccgtcaagc cgtcaattgt ctgattcgtt
     4801 accaattatg acaacttgac ggctacatca ttcacttttt cttcacaacc ggcacggaac
     4861 tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag ttgatcgtca
     4921 aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa aagcagcttc
     4981 gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa ctgctggcgg
     5041 aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct ggcgatatca
     5101 aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac ccgattatcc
     5161 atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa ttgctcaagc
     5221 agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt aatgatttgc
     5281 ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa ccccgtattg
     5341 gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg aaagtaaacc
     5401 cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat ctctcctggc
     5461 gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt tttcaccacc
     5521 ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg tcggtcgata
     5581 aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag atgggcatta
     5641 aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccat
//
