LOCUS       2_His6_MBP_FXR1P_02              8023 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             3622..4719
                     /note="pLannotate"
                     /label="MBP"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(2016..3098)
                     /note="pLannotate"
                     /label="lacI"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             6993..7853
                     /note="pLannotate"
                     /label="AmpR"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      1..589
                     /note="pLannotate"
                     /label="ori"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             complement(1016..1207)
                     /note="pLannotate"
                     /label="rop"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      6616..6846
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="64.52513966480447"
                     /fragment="True"
                     /other="rep_origin"
     misc_feature    775..914
                     /note="pLannotate"
                     /label="bom"
                     /database="addgene"
                     /identity="98.592"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc feature"
     promoter        6889..6992
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="addgene"
                     /identity="99.048"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        complement(3099..3176)
                     /note="pLannotate"
                     /label="lacI promoter"
                     /database="addgene"
                     /identity="97.5"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(1676..1967)
                     /note="pLannotate"
                     /label="TcR"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="24.517212426532325"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(3184..3463)
                     /note="pLannotate"
                     /label="TcR"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="23.509655751469353"
                     /fragment="True"
                     /other="CDS"
     terminator      6532..6579
                     /note="pLannotate"
                     /label="T7 terminator"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     protein_bind    3508..3532
                     /note="pLannotate"
                     /label="lac operator"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein bind"
     RBS             3547..3569
                     /note="pLannotate"
                     /label="RBS"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="RBS"
     CDS             4741..4761
                     /note="pLannotate"
                     /label="TEV site"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             6475..6524
                     /note="pLannotate"
                     /label="SET1"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="39.682539682539684"
                     /fragment="True"
                     /other="CDS"
     promoter        3489..3507
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcaatggtg cactctcagt
      841 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
      901 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
      961 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     1021 ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt
     1081 cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca
     1141 gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt
     1201 tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga
     1261 aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac
     1321 gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg
     1381 gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc
     1441 ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt
     1501 acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc
     1561 agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac
     1621 cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggggccg
     1681 ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga
     1741 aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg
     1801 cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta
     1861 cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc
     1921 accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgagat cccggtgcct
     1981 aatgagtgag ctaacttaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     2041 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     2101 ttgggcgcca gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc
     2161 accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga
     2221 aaatcctgtt tgatggtggt taacggcggg atataacatg agctgtcttc ggtatcgtcg
     2281 tatcccacta ccgagatatc cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt
     2341 gcgcccagcg ccatctgatc gttggcaacc agcatcgcag tgggaacgat gccctcattc
     2401 agcatttgca tggtttgttg aaaaccggac atggcactcc agtcgccttc ccgttccgct
     2461 atcggctgaa tttgattgcg agtgagatat ttatgccagc cagccagacg cagacgcgcc
     2521 gagacagaac ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga
     2581 tgctccacgc ccagtcgcgt accgtcttca tgggagaaaa taatactgtt gatgggtgtc
     2641 tggtcagaga catcaagaaa taacgccgga acattagtgc aggcagcttc cacagcaatg
     2701 gcatcctggt catccagcgg atagttaatg atcagcccac tgacgcgttg cgcgagaaga
     2761 ttgtgcaccg ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg
     2821 ctggcaccca gttgatcggc gcgagattta atcgccgcga caatttgcga cggcgcgtgc
     2881 agggccagac tggaggtggc aacgccaatc agcaacgact gtttgcccgc cagttgttgt
     2941 gccacgcggt tgggaatgta attcagctcc gccatcgccg cttccacttt ttcccgcgtt
     3001 ttcgcagaaa cgtggctggc ctggttcacc acgcgggaaa cggtctgata agagacaccg
     3061 gcatactctg cgacatcgta taacgttact ggtttcacat tcaccaccct gaattgactc
     3121 tcttccgggc gctatcatgc cataccgcga aaggttttgc gccattcgat ggtgtccggg
     3181 atctcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag
     3241 gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc
     3301 cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg
     3361 gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt
     3421 ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatcg atctcgatcc
     3481 cgcgaaatta atacgactca ctatagggga attgtgagcg gataacaatt cccctctaga
     3541 aataattttg tttaacttta agaaggagat atacatatgc accatcatca tcatcattct
     3601 tctggtgtag atctgggtac caaaatcgaa gaaggtaaac tggtaatctg gattaacggc
     3661 gataaaggct ataacggtct cgctgaagtc ggtaagaaat tcgagaaaga taccggaatt
     3721 aaagtcaccg ttgagcatcc ggataaactg gaagagaaat tcccacaggt tgcggcaact
     3781 ggcgatggcc ctgacattat cttctgggca cacgaccgct ttggtggcta cgctcaatct
     3841 ggcctgttgg ctgaaatcac cccggacaaa gcgttccagg acaagctgta tccgtttacc
     3901 tgggatgccg tacgttacaa cggcaagctg attgcttacc cgatcgctgt tgaagcgtta
     3961 tcgctgattt ataacaaaga tctgctgccg aacccgccaa aaacctggga agagatcccg
     4021 gcgctggata aagaactgaa agcgaaaggt aagagcgcgc tgatgttcaa cctgcaagaa
     4081 ccgtacttca cctggccgct gattgctgct gacgggggtt atgcgttcaa gtatgaaaac
     4141 ggcaagtacg acattaaaga cgtgggcgtg gataacgctg gcgcgaaagc gggtctgacc
     4201 ttcctggttg acctgattaa aaacaaacac atgaatgcag acaccgatta ctccatcgca
     4261 gaagctgcct ttaataaagg cgaaacagcg atgaccatca acggcccgtg ggcatggtcc
     4321 aacatcgaca ccagcaaagt gaattatggt gtaacggtac tgccgacctt caagggtcaa
     4381 ccatccaaac cgttcgttgg cgtgctgagc gcaggtatta acgccgccag tccgaacaaa
     4441 gagctggcaa aagagttcct cgaaaactat ctgctgactg atgaaggtct ggaagcggtt
     4501 aataaagaca aaccgctggg tgccgtagcg ctgaagtctt acgaggaaga gttggcgaaa
     4561 gatccacgta ttgccgccac catggaaaac gcccagaaag gtgaaatcat gccgaacatc
     4621 ccgcagatgt ccgctttctg gtatgccgtg cgtactgcgg tgatcaacgc cgccagcggt
     4681 cgtcagactg tcgatgaagc cctgaaagac gcgcagactg attacgatat cccaggtacc
     4741 gagaacctgt acttccaatc caatgccgcg gagctgacgg tggaggttcg cggctctaac
     4801 ggggctttct acaagggatt tatcaaagat gttcatgaag actcccttac agttgttttt
     4861 gaaaataatt ggcaaccaga acgccaggtt ccatttaatg aagttagatt accaccacca
     4921 cctgatataa aaaaagaaat tagtgaagga gatgaagtag aggtatattc aagagcaaat
     4981 gaccaagagc catgtgggtg gtggttggct aaagttcgga tgatgaaagg agaattttat
     5041 gtcattgaat atgctgcttg tgacgctact tacaatgaaa tagtcacatt tgaacgactt
     5101 cggcctgtca atcaaaataa aactgtcaaa aaaaatacct tctttaaatg cacagtggat
     5161 gttcctgagg atttgagaga ggcgtgtgct aatgaaaatg cacataaaga ttttaagaaa
     5221 gcagtaggag catgcagaat tttttaccat ccagaaacaa cacagctaat gatactgtct
     5281 gccagtgaag caactgtgaa gagagtaaac atcttaagtg acatgcattt gcgaagtatt
     5341 cgtacgaagt tgatgcttat gtccagaaat gaagaggcca ctaagcattt agaatgcaca
     5401 aaacaacttg cagcagcttt tcatgaggaa tttgttgtga gagaagattt aatgggcctg
     5461 gcaataggaa cacatggtag taacatccag caagctagga aggttcctgg agttaccgcc
     5521 attgagctag atgaagatac tggaacattc agaatctacg gagagagtgc tgatgctgta
     5581 aaaaaggcta gaggtttctt ggaatttgtg gaggatttta ttcaggttcc taggaatctc
     5641 gttggaaaag taattggaaa aaatggcaaa gttattcaag aaatagtgga caaatctggt
     5701 gtggttcgag tgagaattga aggggacaat gaaaataaat tacccagaga agacggtatg
     5761 gttccatttg tatttgttgg cactaaagaa agcattggaa atgtgcaggt tcttctagag
     5821 tatcatattg cctatctaaa ggaagtagaa cagctaagaa tggaacgcct acagattgat
     5881 gaacagctgc gacagattgg ttctaggtct tatagcggaa gaggcagagg tcgtcgggga
     5941 cctaattaca cctccggtta tggtacaaat tctgagctgt ctaacccctc tgaaacggaa
     6001 tctgagcgta aagacgagct gagtgattgg tcattggcag gagaagatga tcgagacagc
     6061 cgacatcagc gtgacagcag gagacgccca ggaggaagag gcagaagtgt ttcagggggt
     6121 cgaggtcgtg gtggaccacg tggtggcaaa tcctccatca gttctgtgct caaagatcca
     6181 gacagcaatc catacagctt acttgataat acagaatcag atcagactgc agacactgat
     6241 gccagcgaat ctcatcacag tactaaccgt cgtaggcggt ctcgtagacg aaggactgat
     6301 gaagatgctg ttctgatgga tggaatgact gaatctgata cagcttcagt taatgaaaat
     6361 gggctaggca aaagatgtga ttaacattgg aagtggataa cggatccgaa ttcgagctcc
     6421 gtcgacaagc ttgcggccgc actcgagcac caccaccacc accactgaga tccggctgct
     6481 aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa
     6541 ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc
     6601 ggattggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     6661 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     6721 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     6781 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     6841 gttcacatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct
     6901 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     6961 taaatgcttc aataatttga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     7021 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     7081 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     7141 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     7201 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     7261 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     7321 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     7381 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     7441 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     7501 ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca
     7561 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     7621 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     7681 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     7741 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     7801 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     7861 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     7921 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     7981 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttc
//
