LOCUS       3_His6_MBP_FXR2P_P474S__P493N__03              8419 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             3622..4719
                     /note="pLannotate"
                     /label="MBP"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(2016..3098)
                     /note="pLannotate"
                     /label="lacI"
                     /database="addgene"
                     /identity="99.908"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             7389..8249
                     /note="pLannotate"
                     /label="AmpR"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      1..589
                     /note="pLannotate"
                     /label="ori"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             complement(1016..1207)
                     /note="pLannotate"
                     /label="rop"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      7012..7242
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="64.52513966480447"
                     /fragment="True"
                     /other="rep_origin"
     misc_feature    775..914
                     /note="pLannotate"
                     /label="bom"
                     /database="addgene"
                     /identity="98.592"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc feature"
     promoter        7285..7388
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="addgene"
                     /identity="99.048"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        complement(3099..3176)
                     /note="pLannotate"
                     /label="lacI promoter"
                     /database="addgene"
                     /identity="97.5"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(1676..1967)
                     /note="pLannotate"
                     /label="TcR"
                     /database="addgene"
                     /identity="99.658"
                     /match_length="24.517212426532325"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(3184..3463)
                     /note="pLannotate"
                     /label="TcR"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="23.509655751469353"
                     /fragment="True"
                     /other="CDS"
     terminator      6928..6975
                     /note="pLannotate"
                     /label="T7 terminator"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     protein_bind    3508..3532
                     /note="pLannotate"
                     /label="lac operator"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein bind"
     CDS             4735..4758
                     /note="pLannotate"
                     /label="HRV 3C site"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     RBS             3547..3569
                     /note="pLannotate"
                     /label="RBS"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="RBS"
     CDS             6871..6920
                     /note="pLannotate"
                     /label="SET1"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="39.682539682539684"
                     /fragment="True"
                     /other="CDS"
     promoter        3489..3507
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcaatggtg cactctcagt
      841 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
      901 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
      961 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     1021 ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt
     1081 cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca
     1141 gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt
     1201 tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga
     1261 aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac
     1321 gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg
     1381 gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc
     1441 ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt
     1501 acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc
     1561 agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac
     1621 cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggggccg
     1681 ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga
     1741 aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg
     1801 cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta
     1861 cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc
     1921 accggaagga gctgactggg ttgaaggctt tcaagggcat cggtcgagat cccggtgcct
     1981 aatgagtgag ctaacttaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     2041 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     2101 ttgggcgcca gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc
     2161 accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga
     2221 aaatcctgtt tgatggtgga taacggcggg atataacatg agctgtcttc ggtatcgtcg
     2281 tatcccacta ccgagatatc cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt
     2341 gcgcccagcg ccatctgatc gttggcaacc agcatcgcag tgggaacgat gccctcattc
     2401 agcatttgca tggtttgttg aaaaccggac atggcactcc agtcgccttc ccgttccgct
     2461 atcggctgaa tttgattgcg agtgagatat ttatgccagc cagccagacg cagacgcgcc
     2521 gagacagaac ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga
     2581 tgctccacgc ccagtcgcgt accgtcttca tgggagaaaa taatactgtt gatgggtgtc
     2641 tggtcagaga catcaagaaa taacgccgga acattagtgc aggcagcttc cacagcaatg
     2701 gcatcctggt catccagcgg atagttaatg atcagcccac tgacgcgttg cgcgagaaga
     2761 ttgtgcaccg ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg
     2821 ctggcaccca gttgatcggc gcgagattta atcgccgcga caatttgcga cggcgcgtgc
     2881 agggccagac tggaggtggc aacgccaatc agcaacgact gtttgcccgc cagttgttgt
     2941 gccacgcggt tgggaatgta attcagctcc gccatcgccg cttccacttt ttcccgcgtt
     3001 ttcgcagaaa cgtggctggc ctggttcacc acgcgggaaa cggtctgata agagacaccg
     3061 gcatactctg cgacatcgta taacgttact ggtttcacat tcaccaccct gaattgactc
     3121 tcttccgggc gctatcatgc cataccgcga aaggttttgc gccattcgat ggtgtccggg
     3181 atctcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag
     3241 gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc
     3301 cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg
     3361 gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt
     3421 ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatcg atctcgatcc
     3481 cgcgaaatta atacgactca ctatagggga attgtgagcg gataacaatt cccctctaga
     3541 aataattttg tttaacttta agaaggagat atacatatgc accatcatca tcatcattct
     3601 tctggtgtag atctgggtac caaaatcgaa gaaggtaaac tggtaatctg gattaacggc
     3661 gataaaggct ataacggtct cgctgaagtc ggtaagaaat tcgagaaaga taccggaatt
     3721 aaagtcaccg ttgagcatcc ggataaactg gaagagaaat tcccacaggt tgcggcaact
     3781 ggcgatggcc ctgacattat cttctgggca cacgaccgct ttggtggcta cgctcaatct
     3841 ggcctgttgg ctgaaatcac cccggacaaa gcgttccagg acaagctgta tccgtttacc
     3901 tgggatgccg tacgttacaa cggcaagctg attgcttacc cgatcgctgt tgaagcgtta
     3961 tcgctgattt ataacaaaga tctgctgccg aacccgccaa aaacctggga agagatcccg
     4021 gcgctggata aagaactgaa agcgaaaggt aagagcgcgc tgatgttcaa cctgcaagaa
     4081 ccgtacttca cctggccgct gattgctgct gacgggggtt atgcgttcaa gtatgaaaac
     4141 ggcaagtacg acattaaaga cgtgggcgtg gataacgctg gcgcgaaagc gggtctgacc
     4201 ttcctggttg acctgattaa aaacaaacac atgaatgcag acaccgatta ctccatcgca
     4261 gaagctgcct ttaataaagg cgaaacagcg atgaccatca acggcccgtg ggcatggtcc
     4321 aacatcgaca ccagcaaagt gaattatggt gtaacggtac tgccgacctt caagggtcaa
     4381 ccatccaaac cgttcgttgg cgtgctgagc gcaggtatta acgccgccag tccgaacaaa
     4441 gagctggcaa aagagttcct cgaaaactat ctgctgactg atgaaggtct ggaagcggtt
     4501 aataaagaca aaccgctggg tgccgtagcg ctgaagtctt acgaggaaga gttggcgaaa
     4561 gatccacgta ttgccgccac catggaaaac gcccagaaag gtgaaatcat gccgaacatc
     4621 ccgcagatgt ccgctttctg gtatgccgtg cgtactgcgg tgatcaacgc cgccagcggt
     4681 cgtcagactg tcgatgaagc cctgaaagac gcgcagactg gtggtggtgg tagcctggaa
     4741 gttctgttcc agggtccgat gggcggtctg gcgagcggtg gcgatgttga gccgggtctg
     4801 ccggtggaag tgcgtggtag caatggtgcg ttttacaagg gttttgttaa agacgtgcac
     4861 gaggacagcg ttaccatctt ctttgaaaac aactggcaaa gcgaacgtca gatcccgttt
     4921 ggtgacgtgc gtctgccgcc gccggcggac tataacaagg aaatcaccga aggcgatgag
     4981 gtggaagttt atagccgtgc gaacgagcaa gaaccgtgcg gttggtggct ggcgcgtgtt
     5041 cgtatgatga agggtgactt ctacgtgatt gaatatgcgg cgtgcgatgc gacctacaac
     5101 gagatcgtta ccctggaacg tctgcgtccg gttaacccga acccgctggc gaccaaaggt
     5161 agcttcttta aggtgacgat ggcggtgccg gaggatctgc gtgaagcgtg cagcaacgaa
     5221 aacgtgcaca aggagttcaa gaaggcgctg ggcgcgaact gcatcttcct gaacatcacc
     5281 aacagcgagc tgttcatcct gagcaccacc gaggcgccgg ttaaacgtgc gagcctgctg
     5341 ggtgacatgc acttccgtag cctgcgtacc aagctgctgc tgatgagccg taacgaagag
     5401 gcgaccaaac acctggagac cagcaagcaa ctggcggcgg cgtttcagga agagttcacc
     5461 gtgcgtgagg acctgatggg tctggcgatt ggcacccacg gtgcgaacat tcaacaggcg
     5521 cgtaaagttc cgggcgttac cgcgatcgag ctgggtgaag agacctgcac cttccgtatc
     5581 tacggtgaaa ccccggaggc gtgccgtcaa gcgcgtagct acctggagtt tagcgaagat
     5641 agcgtgcaag ttccgcgtaa cctggttggc aaagtgatcg gcaagaacgg taaagtgatt
     5701 caggagatcg tggacaaaag cggtgtggtt cgtgtgcgtg ttgagggtga caacgataag
     5761 aaaaacccgc gtgaagaggg tatggtgccg tttattttcg tgggcacccg tgagaacatc
     5821 agcaacgcgc aagcgctgct ggagtatcac ctgagctacc tgcaagaggt ggaacagctg
     5881 cgtctggagc gtctgcagat tgacgagcaa ctgcgtcaaa ttggcctggg ttttcgtccg
     5941 ccgggtagcg gccgtggtag cggcggtagc gacaaggcgg gttatagcac cgacgaaagc
     6001 agcagcagca gcctgcacgc gacccgtacc tacggtggca gctatggtgg ccgtggtcgt
     6061 ggccgtcgta ccggtggccc ggcgtatggt ccgagcagcg atgtgagcac cgcgagcgaa
     6121 accgagagcg aaaagcgtga agagccgaac cgtgcgggtc cgggtgaccg tgatccgagc
     6181 acccgtggtg aagaaagccg tcgtcgtccg accggtggtc gtggccgtgg tccgaacccg
     6241 gcgccgcgtc cgaccagccg ttacaacagc agcagcatta gcagcgtgct gaaagacccg
     6301 gatagcaacc cgtatagcct gctggacacc agcgaaccgg aaccgccggt ggatagcgag
     6361 ccgggcgaac cgccgccggc gagcgcgcgt cgtcgtcgta gccgtcgtcg tcgtaccgat
     6421 gaagaccgta ccgtgatgga tggcggcctg gaaagcgacg gtccgaacat gaccgagaac
     6481 ggtctggagg acgaaagccg tccgcaacgt cgtaaccgta gccgtcgtcg tcgtaaccgt
     6541 ggtaaccgta ccgatggcag catcagcggt gatcgtcaac cggttaccgt ggcggattac
     6601 atcagccgtg cggagagcca aagccgtcag cgtccgccgc tggagcgtac caaaccgagc
     6661 gaggatagcc tgagcggcca gaaaggtgac agcgtgagca aactgccgaa gggtccgagc
     6721 gagaatggtg aactgagcgc gccgctggaa ctgggtagca tggtgaatgg tgtgagctaa
     6781 cattggaagt ggataacgga tccgaattcg agctccgtcg acaagcttgc ggccgcactc
     6841 gagcaccacc accaccacca ctgagatccg gctgctaaca aagcccgaaa ggaagctgag
     6901 ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc
     6961 ttgaggggtt ttttgctgaa aggaggaact atatccggat tggcgaatgg gacgcgccct
     7021 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     7081 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     7141 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     7201 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acattaacgc ttacaattta
     7261 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     7321 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atttgaaaaa
     7381 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     7441 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     7501 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     7561 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     7621 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     7681 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     7741 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     7801 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     7861 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     7921 ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     7981 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     8041 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     8101 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     8161 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     8221 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     8281 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     8341 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     8401 aaaagatcaa aggatcttc
//
