LOCUS       Rhoc_gRNA__3            2813 bp ds-DNA     circular     13-APR-2023
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    372..417
                     /locus_tag="gRNA scaffold"
                     /label="gRNA scaffold"
                     /ApEinfo_label="gRNA scaffold"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    73..291
                     /locus_tag="U6 promoter"
                     /label="U6 promoter"
                     /ApEinfo_label="U6 promoter"
                     /ApEinfo_fwdcolor="#ff8080"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(724..744)
                     /locus_tag="M13-rev"
                     /label="M13-rev"
                     /ApEinfo_label="M13-rev"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(1112..1794)
                     /locus_tag="ColE1 origin"
                     /label="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    complement(750..772)
                     /locus_tag="LacO"
                     /label="LacO"
                     /ApEinfo_label="LacO"
                     /ApEinfo_fwdcolor="#6495ed"
                     /ApEinfo_revcolor="#6495ed"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(1892..2551)
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="yellow"
                     /ApEinfo_revcolor="yellow"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
       61 ccacctgacg tcGAGGGCCT ATTTCCCATG ATTCCTTCAT ATTTGCATAT ACGATACAAG
      121 GCTGTTAGAG AGATAATTAG AATTAATTTG ACTGTAAACA CAAAGATATT AGTACAAAAT
      181 ACGTGACGTA GAAAGTAATA ATTTCTTGGG TAGTTTGCAG TTTTAAAATT ATGTTTTAAA
      241 ATGGACTATC ATATGCTTAC CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC
      301 TTGTGGAAAG GACGAAACAC Cgagaagact acgatcgcct gGTTTTAGAG CTAGAAATAG
      361 CAAGTTAAAA TAAGGCTAGT CCGTTATCAA CTTGAAAAAG TGGCACCGAG TCGGTGCTTT
      421 TTTTCTAGAC CCAGCTTTCT TGTACAAAGT TGGCATTAga tccgctgcat taatgaatcg
      481 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
      541 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
      601 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccaga
      661 attcGAGCTC ggtacccggg gatcctctag agtcgacctg caggcatgca agcttggcgt
      721 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
      781 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
      841 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc CAGCTGcatt
      901 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
      961 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     1021 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     1081 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     1141 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     1201 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     1261 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     1321 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     1381 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     1441 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     1501 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     1561 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     1621 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     1681 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     1741 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     1801 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     1861 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     1921 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     1981 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     2041 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     2101 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     2161 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     2221 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     2281 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     2341 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     2401 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     2461 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     2521 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     2581 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     2641 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     2701 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     2761 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac ata
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