LOCUS       Fragment_1_in_pENTR4    2986 bp ds-DNA     circular     20-FEB-2023
DEFINITION  .
KEYWORDS    "accession:addgene_vdb_2528"
COMMENT     GENERAL Alt Name: pENTR 4 Analyze: Sequence Bacterial Resistance:
            Kanamycin Catalog Number: 11818-010 Cloning Method: Unknown
            Constitutive: Unspecified Notes: Gateway Entry vector, ccdB gene
            (if present) requires DB3.1 E. coli. Same as pENTR1A except with
            NcoI instead of DraI in MCS. Plasmid Type: Other, Entry Vector
            Size: 2720 Source/Vendor: Invitrogen Stable: Unspecified
            Viral/Non-Viral: Unspecified
FEATURES             Location/Qualifiers
     terminator      106..149
                     /label="rrnB_T1_terminator"
     terminator      281..308
                     /label="rrnB_T2_terminator"
     misc_feature    complement(358..441)
                     /label="attL2"
     misc_feature    complement(358..457)
                     /label="attL1"
     misc_feature    491..914
                     /label="pU6-26"
     misc_feature    915..929
                     /label="Double Bsa1 Site"
     misc_feature    930..1008
                     /label="sgRNA Scaffold 2"
     misc_feature    1009..1200
                     /label="tU6-26"
     misc_feature    1215..1314
                     /label="attL2"
     gene            1437..2246
                     /label="KanR2 (variant)"
     misc_feature    2320..2939
                     /label="pBR322_origin"
ORIGIN
        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCTAGCA TGGATCTCGG
       61 GGACGTCTAA CTACTAAGCG AGAGTAGGGA ACTGCCAGGC ATCAAATAAA ACGAAAGGCT
      121 CAGTCGGAAG ACTGGGCCTT TCGTTTTATC TGTTGTTTGT CGGTGAACGC TCTCCTGAGT
      181 AGGACAAATC CGCCGGGAGC GGATTTGAAC GTTGTGAAGC AACGGCCCGG AGGGTGGCGG
      241 GCAGGACGCC CGCCATAAAC TGCCAGGCAT CAAACTAAGC AGAAGGCCAT CCTGACGGAT
      301 GGCCTTTTTG CGTTTCTACA AACTCTTCCT GTTAGTTAGT TACTTAAGCT CGGGCCCCAA
      361 ATAATGATTT TATTTTGACT GATAGTGACC TGTTCGTTGC AACAAATTGA TAAGCAATGC
      421 TTTTTTATAA TGCCAACTTT GTACAAAAAA GCAGGCTCCA CCATGGGAAC CAATTCAGTC
      481 GACTGGATCC cgacttgcct tccgcacaat acatcatttc ttcttagctt tttttcttct
      541 tcttcgttca tacagttttt ttttgtttat cagcttacat tttcttgaac cgtagctttc
      601 gttttcttct ttttaacttt ccattcggag tttttgtatc ttgtttcata gtttgtccca
      661 ggattagaat gattaggcat cgaaccttca agaatttgat tgaataaaac atcttcattc
      721 ttaagatatg aagataatct tcaaaaggcc cctgggaatc tgaaagaaga gaagcaggcc
      781 catttatatg ggaaagaaca atagtatttc ttatataggc ccatttaagt tgaaaacaat
      841 cttcaaaagt cccacatcgc ttagataaga aaacgaagct gagtttatat acagctagag
      901 tcgaagtagt gATTGAGAGA CCGGTCTCCG TTTtagagct agaaatagca agttaaaata
      961 aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgaaactt ttttttgcaa
     1021 aattttccag atcgatttct tcttcctctg ttcttcggcg ttcaatttct ggggttttct
     1081 cttcgttttc tgtaactgaa acctaaaatt tgacctaaaa aaaatctcaa ataatatgat
     1141 tcagtggttt tgtacttttc agttagttga gttttgcagt tccgatgaga taaaccaata
     1201 CTCGAGATAT CTAGACCCAG CTTTCTTGTA CAAAGTTGGC ATTATAAGAA AGCATTGCTT
     1261 ATCAATTTGT TGCAACGAAC AGGTCACTAT CAGTCAAAAT AAAATCATTA TTTGCCATCC
     1321 AGCTGCAGCT CTGGCCCGTG TCTCAAAATC TCTGATGTTA CATTGCACAA GATAAAAATA
     1381 TATCATCATG AACAATAAAA CTGTCTGCTT ACATAAACAG TAATACAAGG GGTGTTATGA
     1441 GCCATATTCA ACGGGAAACG TCGAGGCCGC GATTAAATTC CAACATGGAT GCTGATTTAT
     1501 ATGGGTATAA ATGGGCTCGC GATAATGTCG GGCAATCAGG TGCGACAATC TATCGCTTGT
     1561 ATGGGAAGCC CGATGCGCCA GAGTTGTTTC TGAAACATGG CAAAGGTAGC GTTGCCAATG
     1621 ATGTTACAGA TGAGATGGTC AGACTAAACT GGCTGACGGA ATTTATGCCT CTTCCGACCA
     1681 TCAAGCATTT TATCCGTACT CCTGGTGATG CATGGTTACT CACCACTGCG ATCCCCGGAA
     1741 AAACAGCATT CCAGGTATTA GAAGAATATC CTGATTCAGG TGAAAATATT GTTGATGCGC
     1801 TGGCAGTGTT CCTGCGCCGG TTGCATTCGA TTCCTGTTTG TAATTGTCCT TTTAACAGCG
     1861 ATCGCGTATT TCGTCTCGCT CAGGCGCAAT CACGAATGAA TAACGGTTTG GTTGATGCGA
     1921 GTGATTTTGA TGACGAGCGT AATGGCTGGC CTGTTGAACA AGTCTGGAAA GAAATGCATA
     1981 AACTTTTGCC ATTCTCACCG GATTCAGTCG TCACTCATGG TGATTTCTCA CTTGATAACC
     2041 TTATTTTTGA CGAGGGGAAA TTAATAGGTT GTATTGATGT TGGACGAGTC GGAATCGCAG
     2101 ACCGATACCA GGATCTTGCC ATCCTATGGA ACTGCCTCGG TGAGTTTTCT CCTTCATTAC
     2161 AGAAACGGCT TTTTCAAAAA TATGGTATTG ATAATCCTGA TATGAATAAA TTGCAGTTTC
     2221 ATTTGATGCT CGATGAGTTT TTCTAATCAG AATTGGTTAA TTGGTTGTAA CATTATTCAG
     2281 ATTGGGCCCC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG
     2341 ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG
     2401 TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA
     2461 GAGCGCAGAT ACCAAATACT GTTCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA
     2521 ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA
     2581 GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC
     2641 AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA
     2701 CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA
     2761 AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC
     2821 CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC
     2881 GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG
     2941 CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTT
//