LOCUS       AAVSB31-v1em-Cterm-PE2max-U6pegRNA        7690 bp ds-DNA     circular SYN 24-MAR-2023
DEFINITION  .
ACCESSION
VERSION     .
KEYWORDS    pX601-GFP.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct.
REFERENCE   1  (bases 1 to 8138)
  AUTHORS   Ye L, Wang J, Tan Y, Beyer AI, Xie F, Muench MO and Kan YW
  TITLE     Genome editing using CRISPR-Cas9 to create the HPFH genotype in
            HSPCs: An approach for treating sickle cell disease and
            beta-thalassemia.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Sep 20;113(38):10661-5. doi:
            10.1073/pnas.1612075113. Epub 2016 Sep 6.
  PUBMED    27601644
REFERENCE   2  (bases 1 to 8138)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jun 20, 2017 from SnapGene Console 4.0.0a8
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     repeat_region   complement(7667..106)
                     /standard_name="3'-ITR pSub201(+)"
     misc_feature    3..2954
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="TGCAGGCAGCTGCGCGCTCGCTCGCTCACTGAGGCC"
     misc_feature    122..282
                     /note="Geneious type: reporter"
                     /standard_name="lacZ_a reporter"
     primer_bind     complement(125..142)
                     /standard_name="Eton M13F(-21)"
     primer_bind     complement(125..142)
                     /standard_name="Genewiz M13F(-21)"
     primer_bind     complement(125..142)
                     /standard_name="QB0046 M13 (-21) Forward"
     primer_bind     complement(125..142)
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="M13 (-21) Forward"
     primer_bind     complement(125..142)
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="QB0046"
     primer_bind     complement(125..141)
                     /standard_name="M13-F (-20)"
     primer_bind     complement(126..141)
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="JRD292"
     primer_bind     complement(134..156)
                     /standard_name="QB0049 M13/pUC Forward"
     primer_bind     complement(134..156)
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="QB0049"
     primer_bind     complement(145..168)
                     /standard_name="Genewiz M13F(-47)"
     primer_bind     complement(145..162)
                     /standard_name="Genewiz M13F(-41)"
     primer_bind     complement(145..161)
                     /standard_name="Eton M13F(-40)"
     primer_bind     complement(145..161)
                     /standard_name="M13-F (-40)"
     primer_bind     complement(145..161)
                     /standard_name="QB0047 M13 (-40)"
     primer_bind     complement(145..161)
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="M13 (-40)"
     primer_bind     complement(145..161)
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="QB0047"
     primer_bind     complement(146..167)
                     /standard_name="M13-F (-46)"
     primer_bind     complement(209..228)
                     /standard_name="LacZ-R (EtonSeq)"
     primer_bind     209..228
                     /standard_name="834_LacZ-fwd1"
     primer_bind     complement(247..268)
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="NMG-236"
     misc_feature    300..606
                     /note="Geneious type: origin of replication"
                     /standard_name="f1 origin"
     primer_bind     309..341
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="NMG-306"
     primer_bind     complement(420..439)
                     /standard_name="835_LacZ-rev1"
     regulatory      799..827
                     /note="Geneious type: promoter"
                     /standard_name="amp prom"
     misc_feature    869..1729
                     /note="Geneious type: marker"
                     /standard_name="amp marker"
     CDS             869..1729
                     /standard_name="Amp-R"
     primer_bind     877..894
                     /standard_name="BP0266"
     primer_bind     complement(882..905)
                     /standard_name="BP0218"
     primer_bind     939..956
                     /standard_name="BP0145"
     primer_bind     complement(970..989)
                     /standard_name="BP0016"
     primer_bind     complement(989..1008)
                     /standard_name="BP0144"
     primer_bind     complement(994..1021)
                     /standard_name="563_Amp_Rev"
     primer_bind     994..1021
                     /standard_name="562_Amp_Fwd"
     primer_bind     994..1021
                     /standard_name="SB166"
     primer_bind     complement(1087..1106)
                     /standard_name="QB0005 Amp-R"
     primer_bind     complement(1087..1106)
                     /standard_name="QB0005; SB165"
     primer_bind     1227..1246
                     /standard_name="BP0212"
     primer_bind     complement(1304..1323)
                     /standard_name="ACK563"
     primer_bind     1427..1449
                     /standard_name="SB332; SB332"
     primer_bind     complement(1541..1560)
                     /standard_name="BP0108"
     primer_bind     1547..1570
                     /standard_name="BP0185"
     primer_bind     complement(1558..1578)
                     /standard_name="BP0036"
     primer_bind     1623..1642
                     /standard_name="BP0015"
     primer_bind     1827..1847
                     /standard_name="BP0220"
     misc_feature    1884..2503
                     /note="Geneious type: origin of replication"
                     /standard_name="pBR322 origin"
     primer_bind     1968..1987
                     /standard_name="832_BR322-fwd1"
     primer_bind     2121..2140
                     /standard_name="QB0767 RepO-R"
     primer_bind     2121..2140
                     /standard_name="SB168; QB0767; BP0150"
     primer_bind     complement(2130..2148)
                     /standard_name="BP0103"
     primer_bind     complement(2149..2168)
                     /standard_name="833_BR322-rev1"
     primer_bind     complement(2172..2194)
                     /standard_name="BP0066"
     primer_bind     complement(2247..2265)
                     /standard_name="QB0766 RepO-F"
     primer_bind     complement(2247..2265)
                     /standard_name="QB0766; SB167"
     primer_bind     2389..2408
                     /standard_name="BP0014"
     primer_bind     2408..2427
                     /standard_name="BR322-F"
     primer_bind     complement(2436..2455)
                     /standard_name="SB278"
     primer_bind     2436..2455
                     /standard_name="SB281"
     primer_bind     2450..2469
                     /standard_name="BP0217"
     primer_bind     2452..2471
                     /standard_name="BP0148"
     primer_bind     2497..2518
                     /standard_name="11_pFYF_Seq_Rev"
     primer_bind     2588..2607
                     /standard_name="VR"
     primer_bind     2642..2659
                     /standard_name="QB0039 L4440"
     primer_bind     2642..2659
                     /standard_name="QB0039"
     primer_bind     2786..2805
                     /standard_name="Lac-F (EtonSeq)"
     primer_bind     2786..2805
                     /standard_name="836_Lac-F"
     primer_bind     2786..2805
                     /standard_name="SB177"
     regulatory      2812..2841
                     /note="Geneious type: promoter"
                     /standard_name="lac prom"
     primer_bind     2854..2877
                     /standard_name="M13-R (-46)"
     primer_bind     2854..2877
                     /standard_name="QB0108 Tac promoter"
     primer_bind     2854..2877
                     /standard_name="QB0108"
     primer_bind     2855..2877
                     /standard_name="QB0050 M13/pUC Reverse"
     primer_bind     2855..2877
                     /standard_name="QB0050"
     primer_bind     2874..2890
                     /standard_name="M13-R (-26)"
     primer_bind     2874..2890
                     /standard_name="Genewiz M13R"
     primer_bind     2874..2890
                     /standard_name="Eton M13R Reverse"
     primer_bind     2874..2890
                     /standard_name="QB0048 M13 Reverse"
     primer_bind     2874..2890
                     /standard_name="SB155; QB0048; SB155"
     primer_bind     complement(2876..2902)
                     /standard_name="654_CAG_fwd"
     repeat_region   2917..3046
                     /standard_name="5'-ITR pSub201(+)"
     primer_bind     3033..3072
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="JRD660"
     primer_bind     3041..3055
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="JRD765"
     source          <3058..>3330
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    3059..7656
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="CTCGAGGCGTTGACATTGATTATTGACTAGTTATTAATAGTA
                     ATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTT
                     ACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAA
                     TGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGA
                     GTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACG
                     CCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGA
                     CCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCAT
                     GGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGA
                     TTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAAC
                     GGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCG
                     TGTACGGTGGGAGGTCTATATAAGCAGAGCTCTCTGGCTAACTACCGGTGCCACCATG
                     GCCCCAAAGAAGAAGCGGAAGGTCGGTATCCACGGAGTCCCAGCAGCCAAGCGGAACT
                     ACATCCTGGGCCTGGACATCGGCATCACCAGCGTGGGCTACGGCATCATCGACTACGA
                     GACACGGGACGTGATCGATGCCGGCGTGCGGCTGTTCAAAGAGGCCAACGTGGAAAAC
                     AACGAGGGCAGGCGGAGCAAGAGAGGCGCCAGAAGGCTGAAGCGGCGGAGGCGGCATA
                     GAATCCAGAGAGTGAAGAAGCTGCTGTTCGACTACAACCTGCTGACCGACCACAGCGA
                     GCTGAGCGGCATCAACCCCTACGAGGCCAGAGTGAAGGGCCTGAGCCAGAAGCTGAGC
                     GAGGAAGAGTTCTCTGCCGCCCTGCTGCACCTGGCCAAGAGAAGAGGCGTGCACAACG
                     TGAACGAGGTGGAAGAGGACACCGGCAACGAGCTGTCCACCAAAGAGCAGATCAGCCG
                     GAACAGCAAGGCCCTGGAAGAGAAATACGTGGCCGAACTGCAGCTGGAACGGCTGAAG
                     AAAGACGGCGAAGTGCGGGGCAGCATCAACAGATTCAAGACCAGCGACTACGTGAAAG
                     AAGCCAAACAGCTGCTGAAGGTGCAGAAGGCCTACCACCAGCTGGACCAGAGCTTCAT
                     CGACACCTACATCGACCTGCTGGAAACCCGGCGGACCTACTATGAGGGACCTGGCGAG
                     GGCAGCCCCTTCGGCTGGAAGGACATCAAAGAATGGTACGAGATGCTGATGGGCCACT
                     GCACCTACTTCCCCGAGGAACTGCGGAGCGTGAAGTACGCCTACAACGCCGACCTGTA
                     CAACGCCCTGAACGACCTGAACAATCTCGTGATCACCAGGGACGAGAACGAGAAGCTG
                     GAATATTACGAGAAGTTCCAGATCATCGAGAACGTGTTCAAGCAGAAGAAGAAGCCCA
                     CCCTGAAGCAGATCGCCAAAGAAATCCTCGTGAACGAAGAGGATATTAAGGGCTACAG
                     AGTGACCAGCACCGGCAAGCCCGAGTTCACCAACCTGAAGGTGTACCACGACATCAAG
                     GACATTACCGCCCGGAAAGAGATTATTGAGAACGCCGAGCTGCTGGATCAGATTGCCA
                     AGATCCTGACCATCTACCAGAGCAGCGAGGACATCCAGGAAGAACTGACCAATCTGAA
                     CTCCGAGCTGACCCAGGAAGAGATCGAGCAGATCTCTAATCTGAAGGGCTATACCGGC
                     ACCCACAACCTGAGCCTGAAGGCCATCAACCTGATCCTGGACGAGCTGTGGCACACCA
                     ACGACAACCAGATCGCTATCTTCAACCGGCTGAAGCTGGTGCCCAAGAAGGTGGACCT
                     GTCCCAGCAGAAAGAGATCCCCACCACCCTGGTGGACGACTTCATCCTGAGCCCCGTC
                     GTGAAGAGAAGCTTCATCCAGAGCATCAAAGTGATCAACGCCATCATCAAGAAGTACG
                     GCCTGCCCAACGACATCATTATCGAGCTGGCCCGCGAGAAGAACTCCAAGGACGCCCA
                     GAAAATGATCAACGAGATGCAGAAGCGGAACCGGCAGACCAACGAGCGGATCGAGGAA
                     ATCATCCGGACCACCGGCAAAGAGAACGCCAAGTACCTGATCGAGAAGATCAAGCTGC
                     ACGACATGCAGGAAGGCAAGTGCCTGTACAGCCTGGAAGCCATCCCTCTGGAAGATCT
                     GCTGAACAACCCCTTCAACTATGAGGTGGACCACATCATCCCCAGAAGCGTGTCCTTC
                     GACAACAGCTTCAACAACAAGGTGCTCGTGAAGCAGGAAGAAAACAGCAAGAAGGGCA
                     ACCGGACCCCATTCCAGTACCTGAGCAGCAGCGACAGCAAGATCAGCTACGAAACCTT
                     CAAGAAGCACATCCTGAATCTGGCCAAGGGCAAGGGCAGAATCAGCAAGACCAAGAAA
                     GAGTATCTGCTGGAAGAACGGGACATCAACAGGTTCTCCGTGCAGAAAGACTTCATCA
                     ACCGGAACCTGGTGGATACCAGATACGCCACCAGAGGCCTGATGAACCTGCTGCGGAG
                     CTACTTCAGAGTGAACAACCTGGACGTGAAAGTGAAGTCCATCAATGGCGGCTTCACC
                     AGCTTTCTGCGGCGGAAGTGGAAGTTTAAGAAAGAGCGGAACAAGGGGTACAAGCACC
                     ACGCCGAGGACGCCCTGATCATTGCCAACGCCGATTTCATCTTCAAAGAGTGGAAGAA
                     ACTGGACAAGGCCAAAAAAGTGATGGAAAACCAGATGTTCGAGGAAAAGCAGGCCGAG
                     AGCATGCCCGAGATCGAAACCGAGCAGGAGTACAAAGAGATCTTCATCACCCCCCACC
                     AGATCAAGCACATTAAGGACTTCAAGGACTACAAGTACAGCCACCGGGTGGACAAGAA
                     GCCTAATAGAGAGCTGATTAACGACACCCTGTACTCCACCCGGAAGGACGACAAGGGC
                     AACACCCTGATCGTGAACAATCTGAACGGCCTGTACGACAAGGACAATGACAAGCTGA
                     AAAAGCTGATCAACAAGAGCCCCGAAAAGCTGCTGATGTACCACCACGACCCCCAGAC
                     CTACCAGAAACTGAAGCTGATTATGGAACAGTACGGCGACGAGAAGAATCCCCTGTAC
                     AAGTACTACGAGGAAACCGGGAACTACCTGACCAAGTACTCCAAAAAGGACAACGGCC
                     CCGTGATCAAGAAGATTAAGTATTACGGCAACAAACTGAACGCCCATCTGGACATCAC
                     CGACGACTACCCCAACAGCAGAAACAAGGTCGTGAAGCTGTCCCTGAAGCCCTACAGA
                     TTCGACGTGTACCTGGACAATGGCGTGTACAAGTTCGTGACCGTGAAGAATCTGGATG
                     TGATCAAAAAAGAAAACTACTACGAAGTGAATAGCAAGTGCTATGAGGAAGCTAAGAA
                     GCTGAAGAAGATCAGCAACCAGGCCGAGTTTATCGCCTCCTTCTACAACAACGATCTG
                     ATCAAGATCAACGGCGAGCTGTATAGAGTGATCGGCGTGAACAACGACCTGCTGAACC
                     GGATCGAAGTGAACATGATCGACATCACCTACCGCGAGTACCTGGAAAACATGAACGA
                     CAAGAGGCCCCCCAGGATCATTAAGACAATCGCCTCCAAGACCCAGAGCATTAAGAAG
                     TACAGCACAGACATTCTGGGCAACCTGTATGAAGTGAAATCTAAGAAGCACCCTCAGA
                     TCATCAAAAAGGGCAAAAGGCCGGCGGCCACGAAAAAGGCCGGCCAGGCAAAAAAGAA
                     AAAGGAGGGCAGAGGATCCCTGCTAACATGTGGTGACGTCGAGGAGAATCCTGGCCCA
                     ATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGG
                     ACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCAC
                     CTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGG
                     CCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACC
                     ACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCG
                     CACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAG
                     GGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCA
                     ACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGC
                     CGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGAC
                     GGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCG
                     TGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAA
                     CGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTC
                     GGCATGGACGAGCTGTACAAGTAAAGCGGCCGAATTCCTAGAGCTCGCTGATCAGCCT
                     CGACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTT
                     GACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCG
                     CATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGG
                     GGGAGGATTGGGAAGAGAATAGCAGGCATGCTGGGGAGGTACCTGAGGGCCTATTTCC
                     CATGATTCCTTCATATTTGCATATACGATACAAGGCTGTTAGAGAGATAATTGGAATT
                     AATTTGACTGTAAACACAAAGATATTAGTACAAAATACGTGACGTAGAAAGTAATAAT
                     TTCTTGGGTAGTTTGCAGTTTTAAAATTATGTTTTAAAATGGACTATCATATGCTTAC
                     CGTAACTTGAAAGTATTTCGATTTCTTGGCTTTATATATCTTGTGGAAAGGACGAAAC
                     ACCGGAGACCACGGCAGGTCTCAGTTTTAGTACTCTGGAAACAGAATCTACTAAAACA
                     AGGCAAAATGCCGTGTTTATCTCGTCAACTTGTTGGCGAGATTT"
     regulatory      3069..>3323
                     /note="Geneious type: promoter"
                     /note="EFS-NS"
                     /standard_name="EFS-NS"
     primer_bind     3253..3273
                     /standard_name="1418_EFS-fwd"
     tRNA            <3324..>3330
                     /standard_name
     tRNA            <3324..3325
                     /standard_name=20
     source          <3324..>3330
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          <3337..>6729
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             3337..3393
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /translation="MKRTADGSEFESPKKKRKV"
                     /standard_name="SV40 NLS"
     primer_bind     complement(3371..3392)
                     /standard_name="AVA027"
     misc_feature    3374..3417
                     /standard_name="HJR9"
     misc_feature    complement(3374..3393)
                     /standard_name="HJR8"
     source          <3394..>3498
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             3394..3498
                     /standard_name="NpuC"
     misc_feature    3451..3459
                     /standard_name="ERD>GEP motif"
     primer_bind     complement(3477..3512)
                     /standard_name="MA024"
     misc_feature    3497..3512
                     /note="Geneious type: USER"
                     /standard_name="USER_53.1"
     primer_bind     3497..3531
                     /standard_name="MA023"
     CDS             <3499..4530
                     /product="nickase mutant of the Cas9 endonuclease from
                     theStreptococcus pyogenes Type II CRISPR/Cas system"
                     /note="generates RNA-guided single strand nicks in DNA
                     dueto the H840A mutation in the HNH catalytic domain"
                     /standard_name="Cas9(R221K N394K H840A)"
     primer_bind     3729..3748
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="PC1504"
     primer_bind     complement(3803..3827)
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="PC0895"
     primer_bind     3965..3989
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="KZ685"
     primer_bind     complement(4052..4074)
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="PC0897"
     primer_bind     complement(4296..4323)
                     /note="Derived using Geneious Prime 2021.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="PC1653"
     primer_bind     4310..4338
                     /note="Derived using Geneious Prime 2021.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="PC1652"
     primer_bind     4424..4443
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="PC1505"
     primer_bind     4465..4489
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="KZ686"
     primer_bind     complement(4501..4527)
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="PC1494"
     primer_bind     complement(4502..4520)
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="PC0233"
     CDS             4531..4632
                     /standard_name="SGGSx2-sv40NLS-SGGSx2"
     CDS             4633..6663
                     /standard_name="RT codon optimized Genscript"
     primer_bind     5052..5071
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="JRDSeq19"
     primer_bind     complement(6377..6395)
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="JRDSeq18"
     primer_bind     6664..6684
                     /standard_name="AVA258"
     CDS             6676..6729
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /standard_name="SV40 NLS"
     misc_difference 6705^6706
                     /standard_name="Insert Ser"
     regulatory      6738..6898
                     /note="Geneious type: terminator"
                     /standard_name="WPRE_Gamma"
     source          <6738..>6986
                     /organism="Cloning vector pAAV-CW3SL-EGFP"
                     /mol_type="other DNA"
                     /db_xref="taxon:1476495"
     primer_bind     complement(6794..6814)
                     /standard_name="QB0112 WPRE-R"
     primer_bind     6794..6814
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="JRD369"
     primer_bind     complement(6794..6814)
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="QB0112"
     primer_bind     complement(6794..6814)
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="WPRE-R"
     primer_bind     complement(6816..6833)
                     /standard_name="pWPTRev"
     primer_bind     6816..6833
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="JRD368"
     primer_bind     complement(6966..6996)
                     /standard_name="412_CW3/bGH_rev"
     primer_bind     complement(6969..6986)
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="JRD370"
     primer_bind     6977..7009
                     /standard_name="411_CW3/bGH_fwd"
     misc_feature    <6987..7197
                     /note="Geneious type: polyA_signal"
                     /standard_name="BGH\pA"
     primer_bind     6987..7007
                     /standard_name="435_bGH_QPCRF1"
     primer_bind     6987..7007
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="JRD363"
     primer_bind     complement(6987..7007)
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="JRD371"
     primer_bind     7013..7031
                     /standard_name="436_bGH_QPCRF2"
     primer_bind     7013..7031
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="BGH_For"
     primer_bind     7013..7031
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="JRD364"
     primer_bind     complement(7136..7160)
                     /standard_name="ACK64 (end)"
     primer_bind     complement(7136..7160)
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="ACK-64"
     primer_bind     complement(7136..7160)
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="ACK64"
     primer_bind     complement(7150..7169)
                     /standard_name="438_bGH_QPCRR2"
     primer_bind     complement(7150..7169)
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="JRD366"
     primer_bind     complement(7155..7173)
                     /standard_name="439_bGH_QPCRR3"
     primer_bind     complement(7155..7173)
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="JRD367"
     primer_bind     complement(7175..7194)
                     /standard_name="437_bGH_QPCRR1"
     primer_bind     complement(7175..7194)
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="JRD365"
     misc_feature    7195..>7220
                     /note="Geneious type: Primer"
                     /standard_name="JRD803"
     primer_bind     7195..7214
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="JRD753"
     misc_feature    7196..>7220
                     /note="Geneious type: Primer"
                     /standard_name="JL1425"
     primer_bind     7202..7243
                     /standard_name="SB284"
     regulatory      complement(7221..7227)
                     /note="Geneious type: terminator"
                     /standard_name="U6 terminator"
     misc_feature    complement(7224..7227)
                     /standard_name="GG_JUNCT_4"
     ncRNA           complement(7228..7264)
                     /standard_name="evoPQ1-trimmed"
     misc_difference 7261..>7264
                     /standard_name="BOTTOM PE Flap Oligo"
     misc_feature    7265..7272
                     /standard_name="linker"
     misc_feature    complement(7273..7285)
                     /standard_name="PBS"
     misc_feature    complement(7286..7297)
                     /standard_name="RT temp"
     misc_feature    7287
                     /standard_name="+2 G to C"
     ncRNA           complement(7298..7373)
                     /standard_name="RNA scaffold"
     misc_difference 7298..7364
                     /standard_name="BOTTOM pegRNA Scaffold Oligo"
     misc_difference complement(<7298..7301)
                     /standard_name="TOP PE Flap Oligo"
     misc_feature    complement(7298..7301)
                     /standard_name="GG_Junct"
     misc_difference complement(7302..7368)
                     /standard_name="Top pegRNA Scaffold Oligo"
     misc_difference 7365..>7373
                     /standard_name="BOTTOM Protospacer Oligo"
     misc_feature    complement(7365..7368)
                     /standard_name="GG_JUNCT_2"
     misc_difference complement(7369..7398)
                     /standard_name="TOP Protospacer Oligo"
     misc_feature    complement(7374..7394)
                     /standard_name="mDNMT1 (same as CBE prot)"
     regulatory      complement(7395..7658)
                     /note="Geneious type: promoter"
                     /standard_name="hU6"
     misc_feature    complement(7395..7398)
                     /standard_name="GG_JUNCT_1"
     primer_bind     7416..7450
                     /standard_name="SB279"
     primer_bind     complement(7416..7450)
                     /standard_name="SB280"
     primer_bind     complement(7639..7684)
                     /standard_name="SB232"
     misc_feature    7690^7691
                     /note="Geneious type: Editing History Deletion"
                     /standard_name="CCACTCCCTCTCTGCGCGCTCGCTCGCTCACTGAGGCCGGGC
                     GACCAAAGGTCGCCCGACGCCCGGGCTTTGCCCGGGCGGCCTCAGTGAGCGAGCGAGC
                     GCGCAGCTGCCTGCAGGGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGT
                     ATTTCACACCGCATACGTCAAAGCAACCATAGTACGCGCCCTGTAGCGGCGCATTAAG
                     CGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCG
                     CCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTC
                     AAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGA
                     CCCCAAAAAACTTGATTTGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACG
                     GTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAA
                     CTGGAACAACACTCAACCCTATCTCGGGCTATTCTTTTGATTTATAAGGGATTTTGCC
                     GATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTT
                     AACAAAATATTAACGTTTACAATTTTATGGTGCACTCTCAGTACAATCTGCTCTGATG
                     CCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGC
                     TTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATG
                     TGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATAC
                     GCCTATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGACGTCAGGTGGCAC
                     TTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAAT
                     ATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGA
                     AGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTG
                     CCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAG
                     TTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGA
                     GTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGG
                     CGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTAT
                     TCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCA
                     TGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAA
                     CTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATG
                     GGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAA
                     ACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATT
                     AACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCG
                     GATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTG
                     ATAAATCTGGAGCCGGTGAGCGTGGAAGCCGCGGTATCATTGCAGCACTGGGGCCAGA
                     TGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGAT
                     GAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGT
                     CAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAA
                     AAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAG
                     TTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATC
                     CTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGT
                     GGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGC
                     AGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCA
                     AGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGC
                     TGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGAT
                     AAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAA
                     CGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCC
                     CGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGC
                     ACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCC
                     ACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAA
                     AAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCAC
                     ATGT"
ORIGIN      
        1 ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac
       61 gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagcctt aattaaccta
      121 attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
      181 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
      241 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg
      301 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
      361 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
      421 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
      481 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
      541 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
      601 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
      661 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
      721 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      781 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      841 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      901 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
      961 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     1021 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     1081 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     1141 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     1201 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     1261 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     1321 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     1381 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     1441 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     1501 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     1561 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     1621 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     1681 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     1741 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1801 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     1861 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1921 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1981 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     2041 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     2101 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     2161 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     2221 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     2281 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     2341 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     2401 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     2461 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     2521 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     2581 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     2641 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     2701 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     2761 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     2821 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     2881 cagctatgac catgattacg ccagatttaa ttaaggctgc gcgctcgctc gctcactgag
     2941 gccgcccggg caaagcccgg gcgtcgggcg acctttggtc gcccggcctc agtgagcgag
     3001 cgagcgcgca gagagggagt ggccaactcc atcactaggg gttcctgcgg cctctagaat
     3061 tcgctagcta ggtcttgaaa ggagtgggaa ttggctccgg tgcccgtcag tgggcagagc
     3121 gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga tccggtgcct
     3181 agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc
     3241 ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca
     3301 acgggtttgc cgccagaaca caggaccggt gccaccatga aacggacagc cgacggaagc
     3361 gagttcgagt caccaaagaa gaagcggaaa gtcatcaaga ttgctacacg gaaatacctg
     3421 ggaaagcaga acgtgtacga catcggcgtg gagcgggatc acaacttcgc cctgaagaat
     3481 ggctttatcg ccagcaattg tttcaacgaa atcggcaagg ctaccgccaa gtacttcttc
     3541 tacagcaaca tcatgaactt tttcaagacc gagattaccc tggccaacgg cgagatccgg
     3601 aagcggcctc tgatcgagac aaacggcgaa accggggaga tcgtgtggga taagggccgg
     3661 gattttgcca ccgtgcggaa agtgctgagc atgccccaag tgaatatcgt gaaaaagacc
     3721 gaggtgcaga caggcggctt cagcaaagag tctatcctgc ccaagaggaa cagcgataag
     3781 ctgatcgcca gaaagaagga ctgggaccct aagaagtacg gcggcttcga cagccccacc
     3841 gtggcctatt ctgtgctggt ggtggccaaa gtggaaaagg gcaagtccaa gaaactgaag
     3901 agtgtgaaag agctgctggg gatcaccatc atggaaagaa gcagcttcga gaagaatccc
     3961 atcgactttc tggaagccaa gggctacaaa gaagtgaaaa aggacctgat catcaagctg
     4021 cctaagtact ccctgttcga gctggaaaac ggccggaaga gaatgctggc ctctgccggc
     4081 gaactgcaga agggaaacga actggccctg ccctccaaat atgtgaactt cctgtacctg
     4141 gccagccact atgagaagct gaagggctcc cccgaggata atgagcagaa acagctgttt
     4201 gtggaacagc acaagcacta cctggacgag atcatcgagc agatcagcga gttctccaag
     4261 agagtgatcc tggccgacgc taatctggac aaagtgctgt ccgcctacaa caagcaccgg
     4321 gataagccca tcagagagca ggccgagaat atcatccacc tgtttaccct gaccaatctg
     4381 ggagcccctg ccgccttcaa gtactttgac accaccatcg accggaagag gtacaccagc
     4441 accaaagagg tgctggacgc caccctgatc caccagagca tcaccggcct gtacgagaca
     4501 cggatcgacc tgtctcagct gggaggtgac tccggcggaa gctctggtgg cagcaagcgg
     4561 accgccgacg gctctgaatt cgagagccct aagaagaaaa gaaaggtgag cggaggctct
     4621 agcggcggaa gcaccctgaa cattgaagac gagtatagac tgcatgaaac aagcaaggaa
     4681 cccgacgtgt ccctgggctc cacctggctg tccgactttc cccaggcctg ggccgagaca
     4741 ggaggaatgg gcctggccgt gcggcaggca cccctgatca tccctctgaa ggccacctct
     4801 acacccgtga gcatcaagca gtaccctatg tctcaggagg ccagactggg catcaagcct
     4861 cacatccaga ggctgctgga ccagggcatc ctggtgccat gccagagccc ctggaacaca
     4921 ccactgctgc ccgtgaagaa gccaggcacc aatgactata gacccgtgca ggatctgaga
     4981 gaggtgaaca agagggtgga ggatatccac cccaccgtgc ccaaccctta caatctgctg
     5041 tccggcctgc ccccttctca ccagtggtat acagtgctgg acctgaagga tgccttcttt
     5101 tgtctgagac tgcaccctac cagccagcca ctgttcgcct ttgagtggag ggaccctgag
     5161 atgggcatct ctggccagct gacctggaca cgcctgcctc agggcttcaa gaatagccca
     5221 acactgttta acgaggccct gcaccgcgac ctggcagatt tccggatcca gcacccagat
     5281 ctgatcctgc tgcagtacgt ggacgatctg ctgctggccg ccaccagcga gctggattgc
     5341 cagcagggaa cacgcgccct gctgcagacc ctgggaaacc tgggatatag ggcatccgcc
     5401 aagaaggccc agatctgtca gaagcaggtg aagtacctgg gctatctgct gaaggagggc
     5461 cagagatggc tgacagaggc caggaaggag acagtgatgg gccagccaac acccaagacc
     5521 ccaagacagc tgagggagtt cctgggcaaa gcaggatttt gcaggctgtt catcccagga
     5581 ttcgcagaga tggcagcacc tctgtaccca ctgaccaagc cgggcaccct gtttaattgg
     5641 ggccctgacc agcagaaggc ctatcaggag atcaagcagg ccctgctgac agcaccagcc
     5701 ctgggcctgc cagacctgac caagcctttc gagctgtttg tggatgagaa gcagggctac
     5761 gccaagggcg tgctgaccca gaagctggga ccatggagac ggcccgtggc ctatctgtcc
     5821 aagaagctgg acccagtggc agcaggatgg ccaccatgcc tgaggatggt ggcagcaatc
     5881 gccgtgctga caaaggatgc cggcaagctg accatgggac agccactggt catcctggca
     5941 ccacacgcag tggaggccct ggtgaagcag cctccagatc gctggctgtc taacgcccgg
     6001 atgacacact accaggccct gctgctggac accgatcgcg tgcagtttgg ccctgtggtg
     6061 gccctgaatc cagccaccct gctgcctctg ccagaggagg gcctgcagca caactgtctg
     6121 gacatcctgg cagaggcaca cggaacaagg ccagacctga ccgatcagcc cctgcctgac
     6181 gccgatcaca catggtatac cgatggaagc tccctgctgc aggagggcca gaggaaggca
     6241 ggagcagcag tgaccacaga gacagaagtg atctgggcca aggccctgcc agcaggcaca
     6301 tccgcccagc gggccgagct gatcgccctg acccaggccc tgaagatggc cgagggcaag
     6361 aagctgaacg tgtacacaga ctccagatat gccttcgcca ccgcacacat ccacggagag
     6421 atctacaggc gccggggctg gctgacctct gagggcaagg agatcaagaa caaggatgag
     6481 atcctggccc tgctgaaggc cctgtttctg cccaagcggc tgagcatcat ccactgtcct
     6541 ggacaccaga agggacactc cgccgaggca aggggcaatc ggatggccga ccaggccgcc
     6601 agaaaggctg ctattactga aactcccgac acttccactc tgctgattga aaactcctcc
     6661 ccttctggcg gctcaaaaag aaccgccgac ggcagcgaat tcgagtctcc caagaagaag
     6721 aggaaagtct aagatctgat aatcaacctc tggattacaa aatttgtgaa agattgactg
     6781 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     6841 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttag
     6901 ttcttgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     6961 tgttgggcac tgacaattcc gtggtgcgac tgtgccttct agttgccagc catctgttgt
     7021 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     7081 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     7141 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     7201 ggtgggctct atggctcgag aaaaaaattc tagttggttt aacgcgtaac tagatagaac
     7261 cgcggtgtgt ttctggagct gttcgccctg gcatcttgca ccgactcggt gccacttttt
     7321 caagttgata acggactagc cttattttaa cttgctattt ctagctctaa aacgcgcgaa
     7381 cagctccagc ccgcggtgtt tcgtcctttc cacaagatat ataaagccaa gaaatcgaaa
     7441 tactttcaag ttacggtaag catatgatag tccattttaa aacataattt taaaactgca
     7501 aactacccaa gaaattatta ctttctacgt cacgtatttt gtactaatat ctttgtgttt
     7561 acagtcaaat taattctaat tatctctcta acagccttgt atcgtatatg caaatatgaa
     7621 ggaatcatgg gaaataggcc ctcttcctgc ccgaccttgc ggccgcagga acccctagtg
     7681 atggagttgg
//
