LOCUS       Exported                4626 bp ds-DNA     circular UNA 14-FEB-2023
DEFINITION  natural linear DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4626)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Feb 21, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     *1,969bp dat-1 promoter inserted into Han's pHW393 backbone.
FEATURES             Location/Qualifiers
     source          1..4626
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     promoter        join(1..2,4566..4626)
                     /locus_tag="decoy ORF"
                     /label=decoy ORF
     misc_feature    37..1051
                     /locus_tag="sre-1 promoter"
                     /label=sre-1 promoter
     misc_feature    37..56
                     /locus_tag="Forward primer"
                     /label=Forward primer
     misc_feature    1015..1051
                     /locus_tag="Reverse primer"
                     /label=Reverse primer
     misc_feature    1097..1138
                     /locus_tag="42bp intron"
                     /label=42bp intron
     misc_feature    1156..1179
                     /locus_tag="NLS"
                     /label=NLS
     misc_feature    1186..1626
                     /locus_tag="SK GAL4"
                     /label=SK GAL4
     misc_feature    1186..1188
                     /locus_tag="ATG start"
                     /label=ATG start
     misc_feature    1684..1716
                     /locus_tag="VP16 domain"
                     /label=VP16 domain
     misc_feature    1723..1755
                     /locus_tag="VP16 domain(1)"
                     /label=VP16 domain
                     /label=VP16 domain(1)
     misc_feature    1762..1794
                     /locus_tag="VP16 domain(2)"
                     /label=VP16 domain
                     /label=VP16 domain(2)
     misc_feature    1801..1833
                     /locus_tag="VP16 domain(3)"
                     /label=VP16 domain
                     /label=VP16 domain(3)
     misc_feature    1879..2311
                     /locus_tag="let-858 3UTR"
                     /label=let-858 3UTR
                     /label=nonstandard type:  UTR
     CDS             2674..3333
                     /locus_tag="AmpR"
                     /label=AmpR
     rep_origin      3431..4113
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
     misc_binding    4453..4475
                     /locus_tag="LacO"
                     /label=LacO
     primer_bind     4481..4501
                     /locus_tag="M13-rev"
                     /label=M13-rev
     misc_feature    4515..4565
                     /locus_tag="decoy intron"
                     /label=decoy intron
ORIGIN
        1 aagcttgcat gcgcggccgc actgactggg ccggccattc tcccgcacgg agtcacacga
       61 gtgttttatt ttttagccca aggcaacaaa aattgcttaa actggaaaat atcaggatat
      121 tccgccatat cgatttctgt ggatatatag aaaatgataa tattcggaac tagtctatgt
      181 tgtccgaaga aaaaaatctt acggcctgag aagtgatacc tgtaagcaat ttgtctacaa
      241 aaaagcctgc aaacaccgac tacagtaccc caaaacaccc gattttaata tattggtcag
      301 ttttcagctg aaaattgatt tttcgaagtt aattgagtgc tgttttcaaa aatcgactgc
      361 aaattgaggt ggaaatgcag gaaatttttt ttttgagagt ttcagcattt tcaatcgaaa
      421 aatgtgaatt ttcatgcgaa aatcatgttt cgcattccca attatccatt tttcccgttt
      481 tcccacaatt ctagcttgaa aataaacatg acagttttgg gattacagta gtcttcaacg
      541 gcacacacct tttcgcatca atcaaaaact tgtcttgtcg tgtcgagacc gggaaccgta
      601 tttttggcag aaaaatcgca aaattttgcg tctgggtagg cattttcgcc cctgcgatgt
      661 gtcggaatat aattttgcag tcacttcttt gttttccatt tttttagctg aatcatccac
      721 tggtaacatt atgtaatgac tatatttata aaaagccgtt ccaaaccaca tacagaaaat
      781 aagcaacaac aagaaaaagc aaagtgaata ttaaaaatag aggataatgt cacactgtca
      841 cactaattag tcattgctga aaacatttaa acctatactt aaaatctttt ctcgtcttca
      901 ctctcctcta tctcgcttat tcaaagaatc taaaaacact agagattgtg ggttgtttct
      961 gaaaaaaaaa cgaattcacc tgtcacagca aacttaaagt tttcttcgaa ttgtgagctc
     1021 atttctcttt tgattttatt ttcattccaa cggcgcgcct ctagaggatc cccgggattg
     1081 gccaaaggac ccaaaggtat gtttcgaatg atactaacat aacatagaac attttcagga
     1141 ggacccttgg ctaggatggg ccctaaaaag aagcgtaaag ctagcatgaa gctgttgtct
     1201 tcaatggaac aagcttgcga catatgccgg ttaaaaaaac tcaggtgctc gaaggaaaag
     1261 cccaagtgtg ccaagtgtct gaagaacaac tcggagtgcc gttattctcc caaggctatg
     1321 aggtccccac tgaccagggc gcatctgaca gaggtagaat caaggttgga aaaattggaa
     1381 gatttgtttc tgctcatgtt tcctcgggaa aacctggaca gcattttgaa catggactct
     1441 ttggacgaag tgaaagtaat gttgaaacaa ttatatatgc aggactatgg tgacacggat
     1501 gctgctatgg atagcttgac ccctatggaa aatggcatgt ctgaagggcc gagaaaacac
     1561 atgaagactg cgacatcatc gccaaaagtg gatgaagatg gaagccaaag tcagttgagt
     1621 gtatcgccgg aattcccgct gcagtctggt ggcggagggg ctcctagggg ttccggacgg
     1681 gctgacgcat tggacgattt tgatctggat atgctgggaa gtgacgccct cgatgatttt
     1741 gaccttgaca tgcttggttc ggatgccctt gatgactttg acctcgacat gctcggcagt
     1801 gacgcccttg atgatttcga cctggacatg ctgattaact aaggtaccga attcgctagc
     1861 cggccataca agtaatccgg atgatcgacg ccaacgtcgt tgaattttca aattttaaat
     1921 actgaatatt tgtttttttt cctattattt atttattctc tttgtgtttt ttttcttgct
     1981 ttctaaaaaa ttaattcaat ccaaatctaa acattttttt ttctctttcc gtctcccaat
     2041 tcgtattccg ctcctctcat ctgaacacaa tgtgcaagtt tatttatctt ctcgctttca
     2101 tttcattagg acgtgggggg aattggtgga agggggaaac acacaaaagg atgatggaaa
     2161 tgaaataagg acacacaata tgcaacaaca ttcaattcag aaatatggag gaaggtttaa
     2221 aagaaaacat aaaaatatat agaggaggaa ggaaaactag taaaaaataa gcaaagaaat
     2281 taggcgaacg atgagaattg tcctcgcttg ggcccaaaag gccttttttt tttggcgccg
     2341 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     2401 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     2461 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     2521 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     2581 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     2641 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     2701 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     2761 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     2821 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     2881 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     2941 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     3001 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     3061 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     3121 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     3181 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     3241 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     3301 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     3361 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     3421 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     3481 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     3541 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     3601 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     3661 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     3721 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     3781 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     3841 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcat
     3901 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     3961 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     4021 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     4081 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     4141 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     4201 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     4261 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     4321 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     4381 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     4441 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
     4501 gattacgcca agctgtaagt ttaaacatga tcttactaac taactattct catttaaatt
     4561 ttcagagctt aaaaatggct gaaatcactc acaacgatgg atacgctaac aacttggaaa
     4621 tgaaat
//
