LOCUS       Exported                6641 bp ds-DNA     circular SYN 15-FEB-2023
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6641)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Feb 21, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     *3,030 bp srd-37 promoter inserted into Han's pHW393 backbone.
FEATURES             Location/Qualifiers
     source          1..6641
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    37..3066
                     /locus_tag="srd-36 Promoter"
                     /label=srd-36 Promoter
     misc_feature    37..64
                     /locus_tag="Forward Primer"
                     /label=Forward Primer
     misc_feature    3030..3066
                     /locus_tag="Reverse Primer"
                     /label=Reverse Primer
     misc_feature    3112..3153
                     /locus_tag="42bp intron"
                     /label=42bp intron
     misc_feature    3171..3194
                     /locus_tag="NLS"
                     /label=NLS
     misc_feature    3201..3641
                     /locus_tag="SK GAL4"
                     /label=SK GAL4
     misc_feature    3201..3203
                     /locus_tag="ATG start"
                     /label=ATG start
     misc_feature    3699..3731
                     /locus_tag="VP16 domain"
                     /label=VP16 domain
     misc_feature    3738..3770
                     /locus_tag="VP16 domain(1)"
                     /label=VP16 domain
                     /label=VP16 domain(1)
     misc_feature    3777..3809
                     /locus_tag="VP16 domain(2)"
                     /label=VP16 domain
                     /label=VP16 domain(2)
     misc_feature    3816..3848
                     /locus_tag="VP16 domain(3)"
                     /label=VP16 domain
                     /label=VP16 domain(3)
     misc_feature    3894..4326
                     /locus_tag="let-858 3UTR"
                     /label=let-858 3UTR
                     /label=nonstandard type:  UTR
     CDS             4689..5348
                     /locus_tag="AmpR"
                     /label=AmpR
     rep_origin      5446..6128
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
     misc_binding    6468..6490
                     /locus_tag="LacO"
                     /label=LacO
     primer_bind     6496..6516
                     /locus_tag="M13-rev"
                     /label=M13-rev
     misc_feature    6530..6580
                     /locus_tag="decoy intron"
                     /label=decoy intron
     promoter        join(6581..6641,1..2)
                     /locus_tag="decoy ORF"
                     /label=decoy ORF
ORIGIN
        1 aagcttgcat gcgcggccgc actgactggg ccggccgatg tatttaattg tgaagcactc
       61 tcccaaaaat atgagttttc ttcgtgtcac attagcactc acatctgtgt ctcaattaat
      121 tattattgtt ttggcgtgtt ttctaaaaat acggtttgtt acaacaaaga accctatgga
      181 aatgtggacg tatggatatt gtcggtattt tgagccttgg ctatgctata cttaccacca
      241 aattgttcag gtaaaaagac tattattgat ctagttgatt tttaaaaaaa gggtttcagc
      301 tgtgtcttct agctagtaac cttacaatat acggaacatt ttttattaaa tacatgatag
      361 taaaatcaaa gtcaatgaat aaaacagtaa tattcagcac atatattgta ttttacttcc
      421 cacttatatt gtcctcggta agtgtgaaat ctctctaaaa ttgaatacaa cttgataaat
      481 tacagatcat tgtcgtgagt attattgaaa cccaaacgtt taactccgag tatgaagata
      541 aactaaattt tgtgaattca ttgagaatta ttaacgcgga ttatcttatt ttttcgtttt
      601 taagtttctc aaattggctt aattcttgca atttgtttat tctcgcgttt tgcatgtttg
      661 ctgctccggc tgtttgttat tactgtcgaa agtaagttgg gtcaagacaa acgccatatt
      721 tcaacaagtg ttcaacattc aagcaagttt atagaccaat catggccagt tttttttttc
      781 agtttcaaag ctttgtcgaa acattctcaa tcaaaaaatt tagataaatc tcaaaaaaat
      841 tcacccaaaa ctaattgttc aagcagaaag aacattctag agcaaccggg aattttttaa
      901 ataggcattt tttatactgc gaacagaatt ttgccgtttt cttttaatgc catagtaaat
      961 gctcaggtgt gtactacaca cttccgtaat ttacccacat cctctactct tcaatttaat
     1021 aattggaatg aaaaacctct taggcttttc agaaaaacgc tagatcatat taatggtcac
     1081 atcgcaagta tttcacttgc acgacaatat caacataaaa gttttatcat ggtaactttt
     1141 tttttggtat atgtagattt attcaacttc tgttcaggat ctcactattc tattaggttg
     1201 agcaaaaagt ctttgcaaaa tttgtacttt tcttcatttc tcagcgttaa ttcatttttt
     1261 tctgattctg tttttttatt tgtatagtac gtaatgtgca tgtttaatgc atttgaaaaa
     1321 acttttgggt gctgttcaaa atgaccgaga atttgctcgc cgaacgccac gtcctcctag
     1381 gagtttttct gtacgagttc ctccaaggct gcaattgcaa cgaagctcgt cgtaacatgt
     1441 gtgcagtgtt aggcaataac tctgtcacct acaacaccat gaagttttgg ttcgaaaagt
     1501 tcacgaaaaa gaactacgat ctcgatgata aaccaagatc agatcgctct cgttcggata
     1561 tcgatgagga tatttcgaga gccctggaag atgattcaag agcaacgtca cgcaaacttt
     1621 ctgcgactct caagcatctc caaagaacca tcatcaagca tctccataga accggaaggg
     1681 tgccaaagtt cggtcaactt gttcctcaca aattgtccga ttctcagaaa aattgctttg
     1741 tgacctctct ctttcgctgt tcactaggaa acgaacgacg gactggatta aggatatcat
     1801 tactggagat gataaatggg tattatatgt tagccatacc aggaaaaaag agtgggtccc
     1861 ggtcgaggaa accgcgaaac ctggcatcaa gtgagaactt caaggaaaaa agtgcttctc
     1921 tcaattcggt gggacagtaa gggtgtcatt tccagagagc ttcttccaaa ctttgctaca
     1981 atcaacgctg gcctatactg catttaattg aaaaaggtgg tccatgctca tcgattacat
     2041 cgccctagag gatcaaagtt gttgctgctc catgaccacg caagaccgca tacaactttg
     2101 aagacccgcc agaagctcca gaccctcaga atccaatttt cgtctcaccc accgtattca
     2161 acggacttgt ctcctactga ctactatatg ttccgccctc tccaaaatca ccttgccggg
     2221 cagaagtttc atgatcgaaa ggtcgtcgaa actgggttgg acgacttctt tgcctcataa
     2281 tcgcaggagt tctaggcgga gggtactgtt caacttccat tgtgttggca agaagtcata
     2341 agcattaatg gtaaatatat tacccattga atcttgttcg ctttgaaaaa atactacttc
     2401 caaaaaaaaa atcaaaattt tgcaaagact ttttgctcaa cctaatatgt atacttcctt
     2461 gtgtgatgta tattcctatt gagcttttgt attactattg tatcagcacg aaagtggaga
     2521 tattgtttct cgaattttcg ttctcgttaa ttgttgcatt tcctacaatg attgatcctc
     2581 tcatatctat atattttgtc actccttatc gaaagctggt gacaaaatgg gttcaaaaag
     2641 aatcagtggg aagtagagta aatacaatgt aactttgctt ttgtttctat gataaagccg
     2701 tccattttga agctaatttt ttgtttttat aataatttta gaaattgcgc gacccgaccc
     2761 atctctaccc acctaatagg cataagcaca acatgactta acgtttcagc taatgacaat
     2821 gatttataca tggttactct acctgataat acttttcatt taggaattca aaacacaagt
     2881 tttccgctgt tcgcattgct gatgtcctca ttagtgaaaa tatttcttag atttcggtta
     2941 catgttactc tcacttcaat aattcttgtt tgtaactcat gtcacgtatg acgacaaaat
     3001 ctgcagaaac tacacgccct ttggtaaaag gataaagtac tagtaatttt tcttgtttct
     3061 tcaaaaggcg cgcctctaga ggatccccgg gattggccaa aggacccaaa ggtatgtttc
     3121 gaatgatact aacataacat agaacatttt caggaggacc cttggctagg atgggcccta
     3181 aaaagaagcg taaagctagc atgaagctgt tgtcttcaat ggaacaagct tgcgacatat
     3241 gccggttaaa aaaactcagg tgctcgaagg aaaagcccaa gtgtgccaag tgtctgaaga
     3301 acaactcgga gtgccgttat tctcccaagg ctatgaggtc cccactgacc agggcgcatc
     3361 tgacagaggt agaatcaagg ttggaaaaat tggaagattt gtttctgctc atgtttcctc
     3421 gggaaaacct ggacagcatt ttgaacatgg actctttgga cgaagtgaaa gtaatgttga
     3481 aacaattata tatgcaggac tatggtgaca cggatgctgc tatggatagc ttgaccccta
     3541 tggaaaatgg catgtctgaa gggccgagaa aacacatgaa gactgcgaca tcatcgccaa
     3601 aagtggatga agatggaagc caaagtcagt tgagtgtatc gccggaattc ccgctgcagt
     3661 ctggtggcgg aggggctcct aggggttccg gacgggctga cgcattggac gattttgatc
     3721 tggatatgct gggaagtgac gccctcgatg attttgacct tgacatgctt ggttcggatg
     3781 cccttgatga ctttgacctc gacatgctcg gcagtgacgc ccttgatgat ttcgacctgg
     3841 acatgctgat taactaaggt accgaattcg ctagccggcc atacaagtaa tccggatgat
     3901 cgacgccaac gtcgttgaat tttcaaattt taaatactga atatttgttt tttttcctat
     3961 tatttattta ttctctttgt gttttttttc ttgctttcta aaaaattaat tcaatccaaa
     4021 tctaaacatt tttttttctc tttccgtctc ccaattcgta ttccgctcct ctcatctgaa
     4081 cacaatgtgc aagtttattt atcttctcgc tttcatttca ttaggacgtg gggggaattg
     4141 gtggaagggg gaaacacaca aaaggatgat ggaaatgaaa taaggacaca caatatgcaa
     4201 caacattcaa ttcagaaata tggaggaagg tttaaaagaa aacataaaaa tatatagagg
     4261 aggaaggaaa actagtaaaa aataagcaaa gaaattaggc gaacgatgag aattgtcctc
     4321 gcttgggccc aaaaggcctt ttttttttgg cgccgacgtc aggtggcact tttcggggaa
     4381 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     4441 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     4501 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     4561 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     4621 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     4681 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     4741 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     4801 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     4861 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     4921 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     4981 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     5041 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     5101 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     5161 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     5221 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     5281 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     5341 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     5401 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     5461 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     5521 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     5581 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     5641 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     5701 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     5761 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     5821 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     5881 cctacaccga actgagatac ctacagcgtg agcattgaga aagcgccacg cttcccgaag
     5941 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     6001 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     6061 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     6121 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     6181 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     6241 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     6301 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     6361 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     6421 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     6481 gataacaatt tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa
     6541 catgatctta ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat
     6601 cactcacaac gatggatacg ctaacaactt ggaaatgaaa t
//
