LOCUS       Exported                4268 bp ds-DNA     circular SYN 14-FEB-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4268)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Feb 21, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     *657 bp promoter for str-1 inserted into Han's pHW393 plasmid using 
            FseI and AscI
FEATURES             Location/Qualifiers
     source          1..4268
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    37..693
                     /locus_tag="str-1 promoter"
                     /label=str-1 promoter
     misc_feature    37..65
                     /locus_tag="Forward primer"
                     /label=Forward primer
     misc_feature    665..693
                     /locus_tag="Reverse Primer"
                     /label=Reverse Primer
     misc_feature    739..780
                     /locus_tag="42bp intron"
                     /label=42bp intron
     misc_feature    798..821
                     /locus_tag="NLS"
                     /label=NLS
     misc_feature    828..1268
                     /locus_tag="SK GAL4"
                     /label=SK GAL4
     misc_feature    828..830
                     /locus_tag="ATG start"
                     /label=ATG start
     misc_feature    1326..1358
                     /locus_tag="VP16 domain"
                     /label=VP16 domain
     misc_feature    1365..1397
                     /locus_tag="VP16 domain(1)"
                     /label=VP16 domain
                     /label=VP16 domain(1)
     misc_feature    1404..1436
                     /locus_tag="VP16 domain(2)"
                     /label=VP16 domain
                     /label=VP16 domain(2)
     misc_feature    1443..1475
                     /locus_tag="VP16 domain(3)"
                     /label=VP16 domain
                     /label=VP16 domain(3)
     misc_feature    1521..1953
                     /locus_tag="let-858 3UTR"
                     /label=let-858 3UTR
                     /label=nonstandard type:  UTR
     CDS             2316..2975
                     /locus_tag="AmpR"
                     /label=AmpR
     rep_origin      3073..3755
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
     misc_binding    4095..4117
                     /locus_tag="LacO"
                     /label=LacO
     primer_bind     4123..4143
                     /locus_tag="M13-rev"
                     /label=M13-rev
     misc_feature    4157..4207
                     /locus_tag="decoy intron"
                     /label=decoy intron
     promoter        join(4208..4268,1..2)
                     /locus_tag="decoy ORF"
                     /label=decoy ORF
ORIGIN
        1 aagcttgcat gcgcggccgc actgactggg ccggcccata gaaacaagat ttaacaagat
       61 tgtagattaa aaaaatgttg aaaattgaaa actactcaaa aaccatgtta tcagagtttt
      121 gttaatttat agcaacaatt ttcaagcagt gctcttaaaa acactatgaa aaatataaat
      181 catcgtgttc gtttttaaaa tttcacatca aggtattctt tatagacccg tatagtttca
      241 aaaggaaggt tggtttgact cttggcttga aaatagaaca attattttat agtagattca
      301 gaaaagacag ctgtccattg ctaggaaact gtgagatact tagatccaaa tgatgtattt
      361 acaggaaaca aataaggaac taaatagtac tccataaatc actagacatc attaatgaag
      421 cctattcaat tgtaatcata gattcctatt tcccaattga tagctatttt tgtgatcttt
      481 atcttatttt ttgaatgaca tctcagaccc tcaactttgt tcacaattca acaaaactcg
      541 tgaatagtca cctatttcca gtaactcaat tagttttcta ttatttgagg tctggtcaac
      601 aattttattt tatttctctc tctctttctc tctctagatc tagattttga gatttttcta
      661 aattcttaac acaaacttca tatcatttga ctaggcgcgc ctctagagga tccccgggat
      721 tggccaaagg acccaaaggt atgtttcgaa tgatactaac ataacataga acattttcag
      781 gaggaccctt ggctaggatg ggccctaaaa agaagcgtaa agctagcatg aagctgttgt
      841 cttcaatgga acaagcttgc gacatatgcc ggttaaaaaa actcaggtgc tcgaaggaaa
      901 agcccaagtg tgccaagtgt ctgaagaaca actcggagtg ccgttattct cccaaggcta
      961 tgaggtcccc actgaccagg gcgcatctga cagaggtaga atcaaggttg gaaaaattgg
     1021 aagatttgtt tctgctcatg tttcctcggg aaaacctgga cagcattttg aacatggact
     1081 ctttggacga agtgaaagta atgttgaaac aattatatat gcaggactat ggtgacacgg
     1141 atgctgctat ggatagcttg acccctatgg aaaatggcat gtctgaaggg ccgagaaaac
     1201 acatgaagac tgcgacatca tcgccaaaag tggatgaaga tggaagccaa agtcagttga
     1261 gtgtatcgcc ggaattcccg ctgcagtctg gtggcggagg ggctcctagg ggttccggac
     1321 gggctgacgc attggacgat tttgatctgg atatgctggg aagtgacgcc ctcgatgatt
     1381 ttgaccttga catgcttggt tcggatgccc ttgatgactt tgacctcgac atgctcggca
     1441 gtgacgccct tgatgatttc gacctggaca tgctgattaa ctaaggtacc gaattcgcta
     1501 gccggccata caagtaatcc ggatgatcga cgccaacgtc gttgaatttt caaattttaa
     1561 atactgaata tttgtttttt ttcctattat ttatttattc tctttgtgtt ttttttcttg
     1621 ctttctaaaa aattaattca atccaaatct aaacattttt ttttctcttt ccgtctccca
     1681 attcgtattc cgctcctctc atctgaacac aatgtgcaag tttatttatc ttctcgcttt
     1741 catttcatta ggacgtgggg ggaattggtg gaagggggaa acacacaaaa ggatgatgga
     1801 aatgaaataa ggacacacaa tatgcaacaa cattcaattc agaaatatgg aggaaggttt
     1861 aaaagaaaac ataaaaatat atagaggagg aaggaaaact agtaaaaaat aagcaaagaa
     1921 attaggcgaa cgatgagaat tgtcctcgct tgggcccaaa aggccttttt tttttggcgc
     1981 cgacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     2041 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     2101 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     2161 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     2221 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     2281 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     2341 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     2401 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     2461 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     2521 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     2581 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     2641 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     2701 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     2761 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     2821 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     2881 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     2941 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     3001 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     3061 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     3121 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     3181 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     3241 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     3301 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     3361 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     3421 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     3481 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     3541 attgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     3601 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     3661 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     3721 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     3781 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     3841 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     3901 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     3961 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     4021 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     4081 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     4141 atgattacgc caagctgtaa gtttaaacat gatcttacta actaactatt ctcatttaaa
     4201 ttttcagagc ttaaaaatgg ctgaaatcac tcacaacgat ggatacgcta acaacttgga
     4261 aatgaaat
//
