LOCUS       Exported                4703 bp ds-DNA     circular SYN 13-FEB-2023
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Feb 21, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     *999 bp promoter for F35D11.1pAH35 Backbone
            Pmyo-2::NLS::cGAL(DBD)::gp41-1-N-intein::let-858 3'UTR
            sequence made by Han
            confirmed by sequencing with oHW10f and oHW233r, Laragen# 125489, at
            20160928
FEATURES             Location/Qualifiers
     source          1..4703
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    37..1035
                     /locus_tag="F35D11.1 Promoter"
                     /label=F35D11.1 Promoter
     misc_feature    37..61
                     /locus_tag="Forward Primer"
                     /label=Forward Primer
     misc_feature    1003..1035
                     /locus_tag="Reverse Primer"
                     /label=Reverse Primer
     misc_signal     1140..1163
                     /locus_tag="NLS"
                     /label=NLS
     CDS             1170..1610
                     /locus_tag="SK GAL4"
                     /label=SK GAL4
     CDS             1653..1916
                     /locus_tag="gp41 N-intein C elegans codon optimzed"
                     /label=gp41 N-intein C elegans codon optimzed
     3'UTR           1956..2388
                     /locus_tag="let-858 3UTR"
                     /label=let-858 3UTR
     CDS             2751..3410
                     /locus_tag="AmpR"
                     /label=AmpR
     rep_origin      3508..4190
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
     misc_binding    4530..4552
                     /locus_tag="LacO"
                     /label=LacO
     primer_bind     4558..4578
                     /locus_tag="M13-rev"
                     /label=M13-rev
     intron          4593..4642
                     /locus_tag="decoy intron"
                     /label=decoy intron
     promoter        join(4643..4703,1..2)
                     /locus_tag="decoy ORF"
                     /label=decoy ORF
ORIGIN
        1 aagcttgcat gcgcggccgc actgactggg ccggccacac aatttacgat gtcgttgttc
       61 gtttttttct aaacttcgaa attccaatca ttttaataaa tgtttatcaa aaaacgtttt
      121 ttttttaatt ttccagggtt ttgagttcca tcaaccaact aatgtcgata gaagcaaatt
      181 ttgcatagtt tcataaaatt ctggaggtac tgtagaagtg taggattact gtagttttcg
      241 aaaaaaaagg ttttgtcgtt ggaaagatat ggatctgagg ttagtgagga gatatgggcg
      301 ggttacggtg attgtatcgt aggattactg tagtttttgg aaaaaagagc atggtcgggg
      361 tagattagtg gtaatgtaga ggtactgtag gattactgta attattgaaa aaagtaaact
      421 tttgactttt gaagggatat taagttgtcg ttgatgatgt tgacgcagta ctgcactagt
      481 actgtaggcg tatttaggag ttctgtaaga ttactgtaat taaaaaagtt aacttttgta
      541 gtagtactgt aaaagggact gtagtacgag aattgactta aacagtgtga tatttttatt
      601 ttttcgataa acttaaccaa aaatgtagac catcagtcag tatcacttgt tcttagcatg
      661 gttcaaaaaa taattaggac aactctacca gtgctcctga ttttctaggc cagctaaatg
      721 tcattcccaa aatacaaaca tgaaaatctg catttcttgt caaacttccc tactctcccg
      781 cttcctcgcg cggtttttga gtccttccgg acatttttcg acgaaacaca catgtagtag
      841 tgtctaaacg attatgaatt gtgtattgtg tcggctctca gagcccattt gaccccgccg
      901 ttttcgccca ttatttcaca tttcgagaag ctgcccccgc tgcacaacta gaaacatatg
      961 tgtgtagctc tgttttctac gatttcaaca tttccgttcg ttctttttaa tctttgaaat
     1021 aaacacatac acaaaggcgc gcctctagag gatccccggg attggccaaa ggacccaaag
     1081 gtatgtttcg aatgatacta acataacata gaacattttc aggaggaccc ttggctagga
     1141 tgggccctaa aaagaagcgt aaagctagca tgaagctgtt gtcttcaatg gaacaagctt
     1201 gcgacatatg ccggttaaaa aaactcaggt gctcgaagga aaagcccaag tgtgccaagt
     1261 gtctgaagaa caactcggag tgccgttatt ctcccaaggc tatgaggtcc ccactgacca
     1321 gggcgcatct gacagaggta gaatcaaggt tggaaaaatt ggaagatttg tttctgctca
     1381 tgtttcctcg ggaaaacctg gacagcattt tgaacatgga ctctttggac gaagtgaaag
     1441 taatgttgaa acaattatat atgcaggact atggtgacac ggatgctgct atggatagct
     1501 tgacccctat ggaaaatggc atgtctgaag ggccgagaaa acacatgaag actgcgacat
     1561 catcgccaaa agtggatgaa gatggaagcc aaagtcagtt gagtgtatcg ccggaattcc
     1621 cgctgcagtc tggtggcgga ggggctccta ggtgcctcga cctcaagacc caagtccaaa
     1681 ccccacaagg aatgaaggag atctccaaca tccaagtcgg agacctcgtc ctctccaaca
     1741 ccggatacaa cgaggtcctc aacgtcttcc caaagtccaa gaagaagtcc tacaagatca
     1801 ccctcgagga cggaaaggag atcatctgct ccgaggagca cctcttccca acccaaaccg
     1861 gagagatgaa catctccgga ggactcaagg agggaatgtg cctctacgtc aaggagtaag
     1921 gtaccgaatt cgctagccgg ccatacaagt aatccggatg atcgacgcca acgtcgttga
     1981 attttcaaat tttaaatact gaatatttgt tttttttcct attatttatt tattctcttt
     2041 gtgttttttt tcttgctttc taaaaaatta attcaatcca aatctaaaca tttttttttc
     2101 tctttccgtc tcccaattcg tattccgctc ctctcatctg aacacaatgt gcaagtttat
     2161 ttatcttctc gctttcattt cattaggacg tggggggaat tggtggaagg gggaaacaca
     2221 caaaaggatg atggaaatga aataaggaca cacaatatgc aacaacattc aattcagaaa
     2281 tatggaggaa ggtttaaaag aaaacataaa aatatataga ggaggaagga aaactagtaa
     2341 aaaataagca aagaaattag gcgaacgatg agaattgtcc tcgcttgggc ccaaaaggcc
     2401 tttttttttt ggcgccgacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     2461 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     2521 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     2581 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     2641 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     2701 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     2761 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     2821 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     2881 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     2941 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     3001 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     3061 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     3121 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     3181 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     3241 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     3301 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     3361 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     3421 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     3481 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     3541 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     3601 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     3661 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     3721 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     3781 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     3841 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     3901 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     3961 acctacagcg tgagcattga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     4021 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     4081 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     4141 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     4201 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     4261 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     4321 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     4381 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     4441 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     4501 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     4561 gaaacagcta tgaccatgat tacgccaagc tgtaagttta aacatgatct tactaactaa
     4621 ctattctcat ttaaattttc agagcttaaa aatggctgaa atcactcaca acgatggata
     4681 cgctaacaac ttggaaatga aat
//
