LOCUS       Exported                3575 bp ds-DNA     circular SYN 16-FEB-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Feb 21, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     *315 bp promoter for Y48G10A.6
            
            pAH36 backbone
            Pmyo-2::NLS::gp41-1-C-intein::cGAL(AD)::let-858 3'UTR
            sequence made by Han
            confirmed by sequencing with oHW10f and oHW233r, Laragen# 125489, at
            20160928
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    13..327
                     /locus_tag="Y48G10A.6 promoter"
                     /label=Y48G10A.6 promoter
     misc_feature    13..32
                     /locus_tag="Forward Primer (Primer A)"
                     /label=Forward Primer (Primer A)
     misc_feature    303..327
                     /locus_tag="Reverse Primer (Primer B)"
                     /label=Reverse Primer (Primer B)
     misc_signal     432..455
                     /locus_tag="NLS"
                     /label=NLS
     CDS             462..575
                     /locus_tag="gp41 C-intein C elegans codon optimzed"
                     /label=gp41 C-intein C elegans codon optimzed
     CDS             576..587
                     /locus_tag="SSDV for intein splicing"
                     /label=SSDV for intein splicing
     CDS             633..665
                     /locus_tag="VP16 domain"
                     /label=VP16 domain
     CDS             672..704
                     /locus_tag="VP16 domain(1)"
                     /label=VP16 domain
                     /label=VP16 domain(1)
     CDS             711..743
                     /locus_tag="VP16 domain(2)"
                     /label=VP16 domain
                     /label=VP16 domain(2)
     CDS             750..782
                     /locus_tag="VP16 domain(3)"
                     /label=VP16 domain
                     /label=VP16 domain(3)
     3'UTR           828..1260
                     /locus_tag="let-858 3UTR"
                     /label=let-858 3UTR
     CDS             1623..2282
                     /locus_tag="AmpR"
                     /label=AmpR
     rep_origin      2380..3062
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
     misc_binding    3402..3424
                     /locus_tag="LacO"
                     /label=LacO
     primer_bind     3430..3450
                     /locus_tag="M13-rev"
                     /label=M13-rev
     intron          3465..3514
                     /locus_tag="decoy intron"
                     /label=decoy intron
     promoter        join(3515..3575,1..2)
                     /locus_tag="decoy ORF"
                     /label=decoy ORF
ORIGIN
        1 aagcttgcat gcttggattt ttggcacttt ttattggcgc caaatttttt ttcggtaaaa
       61 tgcattttaa ggttaaattt aaaaacgtga tccctgatgt cttagttctc caaaaaaaaa
      121 acactttttt ttttcgaaaa ccttttagaa attgatcact tcagtggtgt gtcccccctt
      181 ttgtttgtca gtttttttta aggaaaaatc aatcaaaaaa gggtacaata aatgttaaat
      241 gagacatgtg tgtacctata taaatacgaa atcgaggttt gtgaaaaatt tcaaattttc
      301 cgagaaaaag ttttttccca aaaaaaaggc gcgcctctag aggatccccg ggattggcca
      361 aaggacccaa aggtatgttt cgaatgatac taacataaca tagaacattt tcaggaggac
      421 ccttggctag gatgggccct aaaaagaagc gtaaagctag catgatgttg aagaagattc
      481 tcaaaattga agaactggat gagcgtgagc tcatcgacat cgaggtctcc ggaaaccacc
      541 tcttctacgc caacgacatc ctcacccaca actcctcctc cgatgtactg cagtctggtg
      601 gcggaggggc tcctaggggt tccggacggg ctgacgcatt ggacgatttt gatctggata
      661 tgctgggaag tgacgccctc gatgattttg accttgacat gcttggttcg gatgcccttg
      721 atgactttga cctcgacatg ctcggcagtg acgcccttga tgatttcgac ctggacatgc
      781 tgattaacta aggtaccgaa ttcgctagcc ggccatacaa gtaatccgga tgatcgacgc
      841 caacgtcgtt gaattttcaa attttaaata ctgaatattt gttttttttc ctattattta
      901 tttattctct ttgtgttttt tttcttgctt tctaaaaaat taattcaatc caaatctaaa
      961 catttttttt tctctttccg tctcccaatt cgtattccgc tcctctcatc tgaacacaat
     1021 gtgcaagttt atttatcttc tcgctttcat ttcattagga cgtgggggga attggtggaa
     1081 gggggaaaca cacaaaagga tgatggaaat gaaataagga cacacaatat gcaacaacat
     1141 tcaattcaga aatatggagg aaggtttaaa agaaaacata aaaatatata gaggaggaag
     1201 gaaaactagt aaaaaataag caaagaaatt aggcgaacga tgagaattgt cctcgcttgg
     1261 gcccaaaagg cctttttttt ttggcgccga cgtcaggtgg cacttttcgg ggaaatgtgc
     1321 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     1381 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     1441 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     1501 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     1561 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     1621 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     1681 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     1741 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     1801 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     1861 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     1921 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     1981 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     2041 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     2101 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     2161 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     2221 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     2281 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     2341 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     2401 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     2461 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     2521 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     2581 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     2641 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     2701 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     2761 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     2821 ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa
     2881 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     2941 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     3001 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     3061 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     3121 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     3181 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     3241 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     3301 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     3361 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     3421 aatttcacac aggaaacagc tatgaccatg attacgccaa gctgtaagtt taaacatgat
     3481 cttactaact aactattctc atttaaattt tcagagctta aaaatggctg aaatcactca
     3541 caacgatgga tacgctaaca acttggaaat gaaat
//
