LOCUS       Exported                6367 bp ds-DNA     circular SYN 15-FEB-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6367)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Feb 21, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     *3,083 bp seb-3 promoter inserted into Han's Andrew's pAH36 backbone
            
            pAH36 backbone
            Pmyo-2::NLS::gp41-1-C-intein::cGAL(AD)::let-858 3'UTR
            sequence made by Han
            confirmed by sequencing with oHW10f and oHW233r, Laragen# 125489, at
            20160928
FEATURES             Location/Qualifiers
     source          1..6367
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    37..3119
                     /locus_tag="seb-3 promoter"
                     /label=seb-3 promoter
     misc_feature    37..64
                     /locus_tag="forward primer"
                     /label=forward primer
     misc_feature    3095..3119
                     /locus_tag="reverse primer"
                     /label=reverse primer
     misc_signal     3224..3247
                     /locus_tag="NLS"
                     /label=NLS
     CDS             3254..3367
                     /locus_tag="gp41 C-intein C elegans codon optimzed"
                     /label=gp41 C-intein C elegans codon optimzed
     CDS             3368..3379
                     /locus_tag="SSDV for intein splicing"
                     /label=SSDV for intein splicing
     CDS             3425..3457
                     /locus_tag="VP16 domain"
                     /label=VP16 domain
     CDS             3464..3496
                     /locus_tag="VP16 domain(1)"
                     /label=VP16 domain
                     /label=VP16 domain(1)
     CDS             3503..3535
                     /locus_tag="VP16 domain(2)"
                     /label=VP16 domain
                     /label=VP16 domain(2)
     CDS             3542..3574
                     /locus_tag="VP16 domain(3)"
                     /label=VP16 domain
                     /label=VP16 domain(3)
     3'UTR           3620..4052
                     /locus_tag="let-858 3UTR"
                     /label=let-858 3UTR
     CDS             4415..5074
                     /locus_tag="AmpR"
                     /label=AmpR
     rep_origin      5172..5854
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
     misc_binding    6194..6216
                     /locus_tag="LacO"
                     /label=LacO
     primer_bind     6222..6242
                     /locus_tag="M13-rev"
                     /label=M13-rev
     intron          6257..6306
                     /locus_tag="decoy intron"
                     /label=decoy intron
     promoter        join(6307..6367,1..2)
                     /locus_tag="decoy ORF"
                     /label=decoy ORF
ORIGIN
        1 aagcttgcat gcgcggccgc actgactggg ccggccccag ttcaaattaa ctctacccaa
       61 ctacacatca caagtgctat atagttatat agaaatcgta aggtgtgaga aattaagcaa
      121 ttaaaaccaa agtcaagttg attttttgtt gaaatttgag tcaaccggcg ctgtttgtat
      181 acacatatac aaacaacggt gactcctatt ttttttcgat aacctttgat tcttcaaatt
      241 gttttgttac gcaacaatta aaggatcgta tgatcgtact caattggttg taaaacatgt
      301 ttaaaacttc attttactgt gttttgactt cactttttcc attttctttt cttgtttttt
      361 tggcaactac cgtactcttt gctatttact aagaagttaa ttatggagtg acgggatatg
      421 ttttaggtgt tcgtgtgttg tttcgatttc tcatccatag ctcaaagtgt cacaaacaac
      481 ggttagcaca tgctagaatt ttcctttttt tgttgttttg ttctttctct cttaaaatca
      541 tggtccattg aaatagtatg tgcaatcgaa cgccgtaagt gagaagaaaa cagaagcaaa
      601 tgagtgttct ataaatagag aaattgaata gatttctata aaaactagaa gattaagttc
      661 cccccttctc tctttctttt tcacttttta tttcaaaagt ggttcaacta attatctggg
      721 aaaaaacaac tagaaaaaga aaaattggca caaaaacaga ggaccaataa caaatcaaag
      781 tgtgcatgaa attttgaaaa tctgcaaatg tgcactttat tttaacgtaa cagggaaggt
      841 gacattaaat ttgaaaagtt aagttattta acacctattg agaaaatatg gtacttaagt
      901 gagaattgaa gcggacgctt ttaagggcct aattcttttg actacgatgc atcaacagta
      961 cactcaattt ctaaaataat ggaaatatta tcttcaagat gaaaaaaaac acccaatttt
     1021 cttatgattt gaattaaaaa ttgtgaggtt tttccttttt cgtataattt tcaaacatgt
     1081 caactaagaa ttctggctta agaaacgcca atatggcaaa atatcgtaac agtaattaat
     1141 taaacaccat gaatacattt taacatttct tgtaaaattg tttcattaaa gttaaaagtg
     1201 caattactta cttttcgcat ttcataagtt cataaaaaag aaggcaacta aaatttgttg
     1261 gttttctaag acgttcctat ttaaaggttg agtagcgtca gtggggattt tgtctaaatc
     1321 taaatctaaa tctatcgtga ttcaaaacga ccaaatatca taataaaaca ctccaaaaat
     1381 gatttttcat aatttccgaa gtttttgcaa actgccaaaa ttttgaaaaa tatgagcttt
     1441 tgaggaattt caaagaaatg ccacatgttc cgacccctac aatgttttaa tacaaataat
     1501 taaaacaaaa ttacagtata aaaagtgtag aaaatttttt ttttcaggtc gacttccaaa
     1561 attatgagtg gcaaaaactg agtaactgtc acgttttgac agtaactggc gctactccac
     1621 ctttaagtta catttcaatt tttttaatca acattgtaga ggttcaaaca tgcgatgccc
     1681 aatttttttt tcaaacggcc agttttttgt ttgaaaattt tccgaaactg acttgtttga
     1741 aaatgttcaa aagtacacgt ttttgtgatt tccgcccaca tttgactata atgagtgtat
     1801 tcaaacaaac cccctttgaa gctactcctt ctttaatgct ttaacttcat acaactccta
     1861 aacctacgtg taagctttac ccagctaatc atctattggt tgtccgatca tttgcatctg
     1921 catttccaat catttggtaa ttacacgaca tttcttcact ttaatacccg ggaatactac
     1981 aacaacaaca actttttatg caggccaatc tatcttcatt ttcctgagaa gcaacttttt
     2041 aatatcttta agaataaaca atttttttgg gggggggggg gggggcagcc gacagttggc
     2101 gtgttctttc ttatgcttgg atttttgctc tcagcaaaag ttcattttaa ttcaaaaagt
     2161 tcgagcttgt tcggtcggct tgttcgcctt cctcttcatt tcttttcaac atttcaccaa
     2221 gccaatttga gcaacttcaa ctcctttttc accccccccc cccccccccc ccctccctat
     2281 tccaattggt gtttgagcct tttctattcg agtcttattt ttattgcgag taaacctaga
     2341 agtattattt ccacgccgtg aaaagggact aatgaatgta gtttcctctt tttttttttg
     2401 aagagttctt ggtcgcctct tttttagtgg gttcttagac cttatatgac aaaaggaaac
     2461 ccatgaatca ggatctggca gtgaggcgtg taatcctttc tttcctcacc atttcaaaat
     2521 actttgaagg ttctggcaaa aaccggactc tatatattcc acatcggtgc tcttattctc
     2581 ggtttcatat ttatttatat ttatttttgc tgctgctaaa tcgacttctt acatccgtga
     2641 aaccaacttt aggaatatct agatctgtta ttatgtttgt gatttgcaag cagacatgtc
     2701 aagttataat taatctcaga caatctctaa attgttgcct aatctcaatt gccctttgct
     2761 cgggcatcct agaaatctgt aagccattgg cttttctgtg actgctcttt gttttctcac
     2821 agatctcaat ggtgctacta ctaacggctc aattttcatt cagggcgtcg ggtcgtgccg
     2881 caccattact tgttttattc actctttttt ctcttttttt ttcttcatct cactaaaata
     2941 aaatagtttt gtcccccccc ccccccctct tttgcctctt ttgcccggag catcatcccc
     3001 tccctttttc accccttctt tgccacccac tatgaactcg acgtgttatt gtcattttca
     3061 gtccttttgc gcagatttct tcttcctaca ttgtcaacct ttcagaaact tgttgctcag
     3121 gcgcgcctct agaggatccc cgggattggc caaaggaccc aaaggtatgt ttcgaatgat
     3181 actaacataa catagaacat tttcaggagg acccttggct aggatgggcc ctaaaaagaa
     3241 gcgtaaagct agcatgatgt tgaagaagat tctcaaaatt gaagaactgg atgagcgtga
     3301 gctcatcgac atcgaggtct ccggaaacca cctcttctac gccaacgaca tcctcaccca
     3361 caactcctcc tccgatgtac tgcagtctgg tggcggaggg gctcctaggg gttccggacg
     3421 ggctgacgca ttggacgatt ttgatctgga tatgctggga agtgacgccc tcgatgattt
     3481 tgaccttgac atgcttggtt cggatgccct tgatgacttt gacctcgaca tgctcggcag
     3541 tgacgccctt gatgatttcg acctggacat gctgattaac taaggtaccg aattcgctag
     3601 ccggccatac aagtaatccg gatgatcgac gccaacgtcg ttgaattttc aaattttaaa
     3661 tactgaatat ttgttttttt tcctattatt tatttattct ctttgtgttt tttttcttgc
     3721 tttctaaaaa attaattcaa tccaaatcta aacatttttt tttctctttc cgtctcccaa
     3781 ttcgtattcc gctcctctca tctgaacaca atgtgcaagt ttatttatct tctcgctttc
     3841 atttcattag gacgtggggg gaattggtgg aagggggaaa cacacaaaag gatgatggaa
     3901 atgaaataag gacacacaat atgcaacaac attcaattca gaaatatgga ggaaggttta
     3961 aaagaaaaca taaaaatata tagaggagga aggaaaacta gtaaaaaata agcaaagaaa
     4021 ttaggcgaac gatgagaatt gtcctcgctt gggcccaaaa ggcctttttt ttttggcgcc
     4081 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     4141 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     4201 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     4261 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     4321 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     4381 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     4441 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     4501 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     4561 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     4621 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     4681 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     4741 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     4801 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     4861 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     4921 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     4981 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     5041 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     5101 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     5161 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     5221 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     5281 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     5341 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     5401 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     5461 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     5521 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     5581 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagca
     5641 ttgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     5701 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     5761 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     5821 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     5881 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     5941 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     6001 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     6061 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     6121 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     6181 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
     6241 tgattacgcc aagctgtaag tttaaacatg atcttactaa ctaactattc tcatttaaat
     6301 tttcagagct taaaaatggc tgaaatcact cacaacgatg gatacgctaa caacttggaa
     6361 atgaaat
//
