LOCUS       Exported                6621 bp ds-DNA     circular SYN 15-FEB-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6621)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Feb 21, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     *3010 bp promoter for srg-13 inserted into Han's pHW393 backbone
            
            Clone (2.2) (the Gal4-(sk)-VP64 section) is confirmed by onfirmedy 
            with oHW233r (in the let-858 3'UTR)  laragen #105284 at 20150727) 
            and sequence with oHW10f at 20150728, #105308)
            Insert: ~1.2 kb of pY41C4A.6 promoter
            Vector: pPD117.01 from the Fire vector (digested with HindIII and 
            EcoRI, ~2.8kb band )
            RE: HindIII and EcoRI digest vector pPD117.01 and Gibson Assembly 
            with insert
FEATURES             Location/Qualifiers
     source          1..6621
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    37..3046
                     /locus_tag="srg-13 promoter"
                     /label=srg-13 promoter
     misc_feature    37..60
                     /locus_tag="Forward Primer (Primer A)"
                     /label=Forward Primer (Primer A)
     misc_feature    3019..3046
                     /locus_tag="Reverse Primer (Primer B)"
                     /label=Reverse Primer (Primer B)
     misc_feature    3092..3133
                     /locus_tag="42bp intron"
                     /label=42bp intron
     misc_feature    3151..3174
                     /locus_tag="NLS"
                     /label=NLS
     misc_feature    3181..3621
                     /locus_tag="SK GAL4"
                     /label=SK GAL4
     misc_feature    3181..3183
                     /locus_tag="ATG start"
                     /label=ATG start
     misc_feature    3679..3711
                     /locus_tag="VP16 domain"
                     /label=VP16 domain
     misc_feature    3718..3750
                     /locus_tag="VP16 domain(1)"
                     /label=VP16 domain
                     /label=VP16 domain(1)
     misc_feature    3757..3789
                     /locus_tag="VP16 domain(2)"
                     /label=VP16 domain
                     /label=VP16 domain(2)
     misc_feature    3796..3828
                     /locus_tag="VP16 domain(3)"
                     /label=VP16 domain
                     /label=VP16 domain(3)
     misc_feature    3874..4306
                     /locus_tag="let-858 3UTR"
                     /label=let-858 3UTR
                     /label=nonstandard type:  UTR
     CDS             4669..5328
                     /locus_tag="AmpR"
                     /label=AmpR
     rep_origin      5426..6108
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
     misc_binding    6448..6470
                     /locus_tag="LacO"
                     /label=LacO
     primer_bind     6476..6496
                     /locus_tag="M13-rev"
                     /label=M13-rev
     misc_feature    6510..6560
                     /locus_tag="decoy intron"
                     /label=decoy intron
     promoter        join(6561..6621,1..2)
                     /locus_tag="decoy ORF"
                     /label=decoy ORF
ORIGIN
        1 aagcttgcat gcgcggccgc actgactggg ccggcctgcc tttccgttct actagaacac
       61 gaaaataaaa tatgtgtacg tttgccaaaa aactttcgag ataaaatgtc agaaaagcaa
      121 aaaggacttg gcagaaagaa gcttgagaac agcggggttt ccatatcgac aatcttgtta
      181 ccaagtcttt tacacctgct atgttatgtc tacatcatat tcccatcggc catgcgagag
      241 tgtgctggct gccccttgct taagctcgtt tcaaaaccaa aagtgtgccg ctggataatc
      301 tctactttat aattatagtg tttcctctac caaagtaata attcatcttc gttgaatgaa
      361 aatcgttatc agaatatagt tgaagctcct agaattttta aaaactaaat agatttccag
      421 aagtctctct gtttttgttg ggattagaaa gaggaccgaa ataataatgt gttccatata
      481 gccaattcca ttttctttct taaagttttc gctgaggatc tgagaagaaa ttgctcacat
      541 tgtcgggtga cagtcagtgt gttaaaaatt tggatttttt aataaaaaat gttttgtaac
      601 ctgtttgtcg agaaaattta tcgacatcat gattggttaa aaatatgact tgaaaagtta
      661 gaattaagtt ggattcatga aaatcagttt ttcaaaacgt agaatggtat ttgaacaatt
      721 gatttattat gccccagctc agacgttaac tcctttataa cgcataaatt caaaactgca
      781 accagagaat aagcatgcaa tgagagaaag tatacagtag acaaaggatg accagtacag
      841 taatccatca cattgtgctt ttccagaata tgctgcgaat acaaaaactg aaccgacgat
      901 tgtgctgaaa tttattaaaa ctattgacag acttgggaaa gcttatttca aaattgtact
      961 tttaaacttt ttcttttatt ttctaaaagt aatacaactg atcaaaagca gttattatgg
     1021 agacgcgctt ttcaaaatta atgtgcaaac acgctccacg gcccaatgaa gacattccgc
     1081 ccccaaaagt tgggtctcgt taggggttgg tggcgaaacc gtacattcaa tttttttttg
     1141 ataatttttg ccggtttcac ctgtttcctt tttgttaggt tttccgtaat tttcaatgtt
     1201 atttgtgtcg aaataatgtt gaaaatgcat gtgagtgtga aaagtgtaat aaggatttct
     1261 tcattggatc atcaattttt tatgaatacg ggtgaaaact gcaaaactaa tcgaaagcat
     1321 ttgaacttac ggtattgccg tcaatactta acgagaccta gggctgtgca accggacgtc
     1381 cggttttttc ggacgtccga aaaaccggac gccggacgtc cgaattcgtc cgaattcgtc
     1441 cgattgcaca gccccatttt ggactataat ggtgcgtcca aagtgcaaag tgctcgtcga
     1501 gacccaacta ttgggggcgg agcgttttca tttgtcgtgg gacgcgcttt cacctcgatt
     1561 tcaacaggcg ttgcgtcccc cctaatattt aatgtttcaa attccaaaaa acaagttctc
     1621 actatctaaa atgtttgacc cgaagaaaaa atcacaaacc tgacgaatat tcctaatctg
     1681 caaatgttga gtagaatgaa taaagccatg gattttgatt ttccttgaac ttgttccata
     1741 atacactcga aacttccctg aaggaaaatt aaagcagaat tgacaatcag ccagactgga
     1801 atccgttgat ctactggaca ttggttaact tttactattc cgattaccag gaggtacaca
     1861 ggaatcgatg aaaatattag aaatatcgct gaaatatatt tttagtttat ccgttgatag
     1921 taattacatc tatctttatc tgaatgagaa gcaattttta tatttttgaa aattcaattg
     1981 gcaaactgcc gaattatcga aaaaaaaatg gtattttatt cagaaatttg aagcagtgat
     2041 ttaccacgat gtttaaaatt tcgttgaaaa ttttttttat ttgttcgact tccaaacgaa
     2101 aatattagaa ctcacaatac agcacaatag aacaatacag caacacgcga atagttcccg
     2161 aaacattttc ttcttgcaac aaaaaactga aacttttcta gttctaaact tatctattca
     2221 ttttatatac aagtaagtca ggtgctcgcc ccagattgat aaggtgggtg catctcgaaa
     2281 aagcagcgga agaaactcgt attcagagga acaatttttg tctgccacga tgtttggaat
     2341 gtgctgataa attttactca aaagtgatag agagttgtta agataccatt tagacttcag
     2401 tagacaacgt tcaaaattgt cgattatttt ttaacctaac agtgaatatt gggaaacata
     2461 tagttataaa ataatccagt atcatgtgat ttagatgtgg caataacaag taggcaagta
     2521 ggtaggaact tagatatggg taggcatgtg ggaagttaaa ctgtcgtcaa ttacttgcct
     2581 ttatcgccag tgaacataac acaaataaaa gttctttaaa atctatcaaa tgtaatgttg
     2641 ccaattgaat tcaaaagatt ataaactatt taatatttat tttcaaagat ttcggagtta
     2701 ttgcatatac aatttataca tccgtcaatc tctttcactt tcgtgacata tcaatctaat
     2761 gggtaaattg aatgtctaaa agtgatattc aatcagaagc aagttgccag gaaatcacca
     2821 aaaacccaca tgcttctcaa ttttcttcct tgttgtaatt ccccataata aaaatttcaa
     2881 ttaatgctta ttacatttta catgagaatt ttgttttcgg cttcatatag aaattcatag
     2941 aatcattttt ggaataattc cggtccataa ttacctgact aaatacgcgc tttgatattt
     3001 ggtatcacat aaaacaaccg agctaaaaat tacagcccac tttattggcg cgcctctaga
     3061 ggatccccgg gattggccaa aggacccaaa ggtatgtttc gaatgatact aacataacat
     3121 agaacatttt caggaggacc cttggctagg atgggcccta aaaagaagcg taaagctagc
     3181 atgaagctgt tgtcttcaat ggaacaagct tgcgacatat gccggttaaa aaaactcagg
     3241 tgctcgaagg aaaagcccaa gtgtgccaag tgtctgaaga acaactcgga gtgccgttat
     3301 tctcccaagg ctatgaggtc cccactgacc agggcgcatc tgacagaggt agaatcaagg
     3361 ttggaaaaat tggaagattt gtttctgctc atgtttcctc gggaaaacct ggacagcatt
     3421 ttgaacatgg actctttgga cgaagtgaaa gtaatgttga aacaattata tatgcaggac
     3481 tatggtgaca cggatgctgc tatggatagc ttgaccccta tggaaaatgg catgtctgaa
     3541 gggccgagaa aacacatgaa gactgcgaca tcatcgccaa aagtggatga agatggaagc
     3601 caaagtcagt tgagtgtatc gccggaattc ccgctgcagt ctggtggcgg aggggctcct
     3661 aggggttccg gacgggctga cgcattggac gattttgatc tggatatgct gggaagtgac
     3721 gccctcgatg attttgacct tgacatgctt ggttcggatg cccttgatga ctttgacctc
     3781 gacatgctcg gcagtgacgc ccttgatgat ttcgacctgg acatgctgat taactaaggt
     3841 accgaattcg ctagccggcc atacaagtaa tccggatgat cgacgccaac gtcgttgaat
     3901 tttcaaattt taaatactga atatttgttt tttttcctat tatttattta ttctctttgt
     3961 gttttttttc ttgctttcta aaaaattaat tcaatccaaa tctaaacatt tttttttctc
     4021 tttccgtctc ccaattcgta ttccgctcct ctcatctgaa cacaatgtgc aagtttattt
     4081 atcttctcgc tttcatttca ttaggacgtg gggggaattg gtggaagggg gaaacacaca
     4141 aaaggatgat ggaaatgaaa taaggacaca caatatgcaa caacattcaa ttcagaaata
     4201 tggaggaagg tttaaaagaa aacataaaaa tatatagagg aggaaggaaa actagtaaaa
     4261 aataagcaaa gaaattaggc gaacgatgag aattgtcctc gcttgggccc aaaaggcctt
     4321 ttttttttgg cgccgacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     4381 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     4441 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     4501 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     4561 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     4621 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     4681 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     4741 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     4801 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     4861 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     4921 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     4981 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     5041 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     5101 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     5161 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     5221 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     5281 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     5341 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     5401 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     5461 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     5521 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     5581 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     5641 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     5701 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     5761 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     5821 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     5881 ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     5941 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     6001 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     6061 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     6121 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     6181 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     6241 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     6301 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     6361 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     6421 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     6481 aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta ctaactaact
     6541 attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac gatggatacg
     6601 ctaacaactt ggaaatgaaa t
//
