LOCUS       Exported                5655 bp ds-DNA     circular SYN 15-FEB-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Feb 21, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     *2371 bp promoter for srb-6 inserted into Andrew's pAH36 backbone
            
            pAH36,
            sequence made by Han
            confirmed by sequencing with oHW10f and oHW233r, Laragen# 125489, at
            20160928
FEATURES             Location/Qualifiers
     source          1..5655
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    37..2407
                     /locus_tag="srb-6 Promoter"
                     /label=srb-6 Promoter
     misc_feature    37..61
                     /locus_tag="Forward Primer (Primer A)"
                     /label=Forward Primer (Primer A)
     misc_feature    2385..2407
                     /locus_tag="Reverse Primer (Primer B)"
                     /label=Reverse Primer (Primer B)
     misc_signal     2512..2535
                     /locus_tag="NLS"
                     /label=NLS
     CDS             2542..2655
                     /locus_tag="gp41 C-intein C elegans codon optimzed"
                     /label=gp41 C-intein C elegans codon optimzed
     CDS             2656..2667
                     /locus_tag="SSDV for intein splicing"
                     /label=SSDV for intein splicing
     CDS             2713..2745
                     /locus_tag="VP16 domain"
                     /label=VP16 domain
     CDS             2752..2784
                     /locus_tag="VP16 domain(1)"
                     /label=VP16 domain
                     /label=VP16 domain(1)
     CDS             2791..2823
                     /locus_tag="VP16 domain(2)"
                     /label=VP16 domain
                     /label=VP16 domain(2)
     CDS             2830..2862
                     /locus_tag="VP16 domain(3)"
                     /label=VP16 domain
                     /label=VP16 domain(3)
     3'UTR           2908..3340
                     /locus_tag="let-858 3UTR"
                     /label=let-858 3UTR
     CDS             3703..4362
                     /locus_tag="AmpR"
                     /label=AmpR
     rep_origin      4460..5142
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
     misc_binding    5482..5504
                     /locus_tag="LacO"
                     /label=LacO
     primer_bind     5510..5530
                     /locus_tag="M13-rev"
                     /label=M13-rev
     intron          5545..5594
                     /locus_tag="decoy intron"
                     /label=decoy intron
     promoter        join(5595..5655,1..2)
                     /locus_tag="decoy ORF"
                     /label=decoy ORF
ORIGIN
        1 aagcttgcat gcgcggccgc actgactggg ccggccccac agttactgtt gttacagact
       61 ctacatctat taatataatg cggtcactct acatcatcag cctgacaatc ctcgacatag
      121 tactttgcat ggctccaatg cctcatattg attggtgtct ttattggaga tatttcaata
      181 aggatcaatt taggcccgaa tgtaacggag tgtaccgtct gaggtttgcg aaacataaag
      241 gtgaggaaaa aagtttttat taacttttta aagaaaattg ataatcaatt ctttcattca
      301 ttcttctcat tcagcataat tcctaattcc agctcacgat catcgacccc gagtaacccc
      361 tcaactatca agtgtcccat ggacaccagc agcaccagtt ccaagattca actcttttgt
      421 caatacgaac tctataatca taagcaatga aaatggattt ggtaagagtt gaaagttaaa
      481 ttaaagccag aatgaaattt ctaagttgcc atcttaaaat caaatgttaa tgcatttcag
      541 gagagtgtcg accgcatcat acaaattgcc agcaatccgg acaatgttca tcaaatcaat
      601 tatgcataga cagtaatgga tattgttgtg gcaataaaaa gtaggttaag caattgattt
      661 tgaaatcaca taatttccaa tttatttttt tcctaacgaa acttagtttt ttttaatgag
      721 aatacaaaaa tagaaatcga aatttccaga ataataaccg aatgcccatc accatcctct
      781 cttgttgctc attgtgcatt ccgtcgtcgt caagtgaatt ggtgtgaatc agattccgaa
      841 tgtgcaccac acttggctcc acgtcagaac agatttttct ttaacgacgc cacagttctg
      901 gacagcattc agaagtctcc atcactttgc tgcccgaccc aatgtggtta taatgtgtgt
      961 gttcgatagg cttctggtat tgtgctcttt atacttgaaa gagcacaaca cacctgtgac
     1021 cagagtcctc atgacaagaa ttttttttca gtatttgtat atttcgatga gaaaaaaaca
     1081 cactttttgt atttttagtt tggtcagatc tttgccaaaa ctgctgaact tttgagcaaa
     1141 actatttgac gcatgacttt cattctttgc gattgatgtg ctatgcagat tttcttgaaa
     1201 ctttagcata ccgaaatttt attaattaaa agcaattcgg tcttttcgaa ataatttttt
     1261 taaacctcga ttaatatata ttacttcctt ttctccccct ttttatgtat gttttatgat
     1321 tgtgatttcc aatacgtgta cattcttgat taatttattc tctccagcaa caagttgcaa
     1381 attggaatta gagagtcagt gttagagaaa tacgagtttc cctcttttta atgtaaagtt
     1441 tattgtttca ccactcgtgc aggtgttaac atgctttttc gatcgccctt acgaactttt
     1501 ttatttatat gtcaataaat attttactga tttcataaaa ttatttttaa tcatttattt
     1561 actattttaa aaaaaattaa aatgttgcat gattttaata accaaaatta ctaattttaa
     1621 aaagaaacaa cactggtttt atgttgtctg atctgagaaa ttcgttagtg aaatacaaga
     1681 aaaatagcaa aaataactat gtatgtacaa aataataggg caccaacaaa aatatttttc
     1741 tgtaatatat ttgtcatggt tttctggatt ttaaaacact caaaatatta tcttgaatga
     1801 gcaaggcata gcttaatcta gagaaaatga tgtgagctga tgtgaacaga aaaaagatac
     1861 gttccaaaaa gtcaccaatt ttcattgtat tatttaccag cattaatttt tttaaatcaa
     1921 ggtagagtag cgtcagctag gaaattgtta aaacctggat aaaattgcca gttattataa
     1981 aaagcatttc aaaaataatt ttaaattttc taatagtagt caaaaattgg tggttattca
     2041 gttttgataa ttcgtaattt aggaaaacta ccgttttttt tttcaaaatt ttacaagaag
     2101 cttttgacta gaaattttaa aataactgaa atgttttgga gggaatcagt atgaaatttg
     2161 gtatttttgg attattttat atctatttag ataaaattgg tgccgtttct ttgactattt
     2221 gttgttcaaa agaccagcag accaaaagct aagcttaaaa tttctgcata tatttcagtt
     2281 cttttttaca tcgtaaaaga aaaaattcta atctttttac ctccattgcg acattttccc
     2341 agactttatg catcaaacat acctcctttt ttgttttcta gaaactactg aaccggatat
     2401 atgccagggc gcgcctctag aggatccccg ggattggcca aaggacccaa aggtatgttt
     2461 cgaatgatac taacataaca tagaacattt tcaggaggac ccttggctag gatgggccct
     2521 aaaaagaagc gtaaagctag catgatgttg aagaagattc tcaaaattga agaactggat
     2581 gagcgtgagc tcatcgacat cgaggtctcc ggaaaccacc tcttctacgc caacgacatc
     2641 ctcacccaca actcctcctc cgatgtactg cagtctggtg gcggaggggc tcctaggggt
     2701 tccggacggg ctgacgcatt ggacgatttt gatctggata tgctgggaag tgacgccctc
     2761 gatgattttg accttgacat gcttggttcg gatgcccttg atgactttga cctcgacatg
     2821 ctcggcagtg acgcccttga tgatttcgac ctggacatgc tgattaacta aggtaccgaa
     2881 ttcgctagcc ggccatacaa gtaatccgga tgatcgacgc caacgtcgtt gaattttcaa
     2941 attttaaata ctgaatattt gttttttttc ctattattta tttattctct ttgtgttttt
     3001 tttcttgctt tctaaaaaat taattcaatc caaatctaaa catttttttt tctctttccg
     3061 tctcccaatt cgtattccgc tcctctcatc tgaacacaat gtgcaagttt atttatcttc
     3121 tcgctttcat ttcattagga cgtgggggga attggtggaa gggggaaaca cacaaaagga
     3181 tgatggaaat gaaataagga cacacaatat gcaacaacat tcaattcaga aatatggagg
     3241 aaggtttaaa agaaaacata aaaatatata gaggaggaag gaaaactagt aaaaaataag
     3301 caaagaaatt aggcgaacga tgagaattgt cctcgcttgg gcccaaaagg cctttttttt
     3361 ttggcgccga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     3421 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     3481 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     3541 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     3601 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     3661 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     3721 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     3781 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     3841 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     3901 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     3961 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     4021 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     4081 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     4141 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     4201 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     4261 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     4321 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     4381 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     4441 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     4501 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     4561 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     4621 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     4681 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     4741 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     4801 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     4861 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     4921 cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     4981 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     5041 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     5101 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     5161 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     5221 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     5281 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     5341 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     5401 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     5461 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     5521 tatgaccatg attacgccaa gctgtaagtt taaacatgat cttactaact aactattctc
     5581 atttaaattt tcagagctta aaaatggctg aaatcactca caacgatgga tacgctaaca
     5641 acttggaaat gaaat
//
