LOCUS       Exported                5675 bp ds-DNA     circular SYN 15-FEB-2023
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5675)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Feb 21, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     *2064 bp promoter for nlp-48 inserted into Han's pHW393 
            backbonepHW393
            Clone (2.2) (the Gal4-(sk)-VP64 section) is confirmed by onfirmedy 
            with oHW233r (in the let-858 3'UTR)  laragen #105284 at 20150727) 
            and sequence with oHW10f at 20150728, #105308)
            Insert: ~1.2 kb of pY41C4A.6 promoter
            Vector: pPD117.01 from the Fire vector (digested with HindIII and 
            EcoRI, ~2.8kb band )
            RE: HindIII and EcoRI digest vector pPD117.01 and Gibson Assembly 
            with insert
FEATURES             Location/Qualifiers
     source          1..5675
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    37..2100
                     /locus_tag="nlp-48 Promoter"
                     /label=nlp-48 Promoter
     misc_feature    37..59
                     /locus_tag="Forward Primer (Primer A)"
                     /label=Forward Primer (Primer A)
     misc_feature    2077..2100
                     /locus_tag="Reverse Primer"
                     /label=Reverse Primer
     misc_feature    2146..2187
                     /locus_tag="42bp intron"
                     /label=42bp intron
     misc_feature    2205..2228
                     /locus_tag="NLS"
                     /label=NLS
     misc_feature    2235..2675
                     /locus_tag="SK GAL4"
                     /label=SK GAL4
     misc_feature    2235..2237
                     /locus_tag="ATG start"
                     /label=ATG start
     misc_feature    2733..2765
                     /locus_tag="VP16 domain"
                     /label=VP16 domain
     misc_feature    2772..2804
                     /locus_tag="VP16 domain(1)"
                     /label=VP16 domain
                     /label=VP16 domain(1)
     misc_feature    2811..2843
                     /locus_tag="VP16 domain(2)"
                     /label=VP16 domain
                     /label=VP16 domain(2)
     misc_feature    2850..2882
                     /locus_tag="VP16 domain(3)"
                     /label=VP16 domain
                     /label=VP16 domain(3)
     misc_feature    2928..3360
                     /locus_tag="let-858 3UTR"
                     /label=let-858 3UTR
                     /label=nonstandard type:  UTR
     CDS             3723..4382
                     /locus_tag="AmpR"
                     /label=AmpR
     rep_origin      4480..5162
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
     misc_binding    5502..5524
                     /locus_tag="LacO"
                     /label=LacO
     primer_bind     5530..5550
                     /locus_tag="M13-rev"
                     /label=M13-rev
     misc_feature    5564..5614
                     /locus_tag="decoy intron"
                     /label=decoy intron
     promoter        join(5615..5675,1..2)
                     /locus_tag="decoy ORF"
                     /label=decoy ORF
ORIGIN
        1 aagcttgcat gcgcggccgc actgactggg ccggccagtg agagatagtt gaacacagca
       61 tttcgacgtg ggtggatgct cctgaaactt ttaaaagata aatacttccg tttgttgact
      121 ttaaatattc agtatttgat ctcttaatgg acatgaactt atccataaga ttcgtgattt
      181 ccgcttctgg gggtgtatcc gaaaagggaa attacgtttt cctagtaaaa tgtgcaaagc
      241 ttttatattc atccttgtaa agtcgaagtc tttctaacta aaaggacgct ataataaact
      301 caaaatggac caaaaaaata tcaagacttt tccaaaacaa aaaaattaac ccacatttta
      361 ttcaatcact gtttcaattt cgcaatttca tcaacaacac acattaccat tattaggaat
      421 ttcaaatttt ttttgaaaag tcttgttaaa aacattttct tttagggtcg ttttgagtag
      481 aatacatttt ttgtacgaaa atctcaaata atcactttca ctatccagac tgacgtattt
      541 cattcacaca tccccgaaag acgagatttt gtgctttgaa cactaactcc tacacctttc
      601 ttttgcattc ttgtagcatt ctgcttccga tattcacgtc acacatgact tggttgactc
      661 acattgttcc cgagatcttg aaagtgtgaa acaagaattc atcacttttg ttttcctgat
      721 aatccaaaaa aacaagttca ttgtttttat ctagcgcttc tcaaattata attctgactg
      781 gcggtgctct tttattatga aaattttttt tgcaataagt tctttggtgc accatgttca
      841 catattctat aaaatttcaa gaattaaaaa cccagagggg gtccttaaaa tatttttagc
      901 gttttgctca aaacacttcg tttattcttg gctgtccccc tcttggtctt atgatcatcc
      961 actttttttt ttcgaaaaaa aaatacacgt catagtaagg tcttcaggag gacctcccga
     1021 tttagaccta ccactcctcc aatcgtgtga ctagattttc aatatttatt atatattcta
     1081 cggatgaata gagaattttg tgacatctgc acgggctctc agatttatat tcagttgcaa
     1141 aaagaaagtt caatacacgg ttaattgggt tttattattt cttattttca tccttgtgac
     1201 gtttggattt cgtttttttt ttcaaatatt ttttttcgat tctcggtttc tcaaaattct
     1261 gcagaacttc tcgtcttgac caaataattc tcaaaaaagc tggttctaat tatttcgtga
     1321 cgtgacttat atcgcgaaat tttcacatcg aagaagaaga aaaatatcaa aattatatta
     1381 ttcgaaaaca agtggttttg tggaattttg tgaatcatat tatgaataag ctcaaaattg
     1441 tttcagattt tggtaaattg cttaatttcc aaacaatgtg gactttcaaa aaattacgag
     1501 aattccatat tgagtgttaa tatctctaaa atgatagtac aaaaagttta aactaaattt
     1561 aaatttgaaa aaaaaatcgc cgaaaagaaa cgtttttttc agttgactca cttgagtcag
     1621 ttgagtccaa ccaattttgc tacaatttta cgcgaatttt tagttttcag aaactttcgt
     1681 ataactatgc aggtatatta ccagttaatt agactaattt gtgtggtttt ttttcaattc
     1741 caaaaatgtg gacgatactt taatattaaa atccacgagt ctgcttttta gtcattttac
     1801 aatttcgaaa aaaaaaatcg gcgccattgt tattgaagga agcattttgc atttccggct
     1861 caattctcaa gtcacaggga cccactcatt ttgtagtcta cggcgaaaaa aggaatttct
     1921 atttggtatt ggtgtatagt attcgtcttc ttcttcaatt cgcatccatc cgtgaacacc
     1981 ctcctcccga aaaatcccgc ccctattatt tcatgatttc tactagtttt ctcttgttgt
     2041 gtcaattcga gaatatttta ttattttaat ttattttctg gtttccttca gaaaatgatg
     2101 ggcgcgcctc tagaggatcc ccgggattgg ccaaaggacc caaaggtatg tttcgaatga
     2161 tactaacata acatagaaca ttttcaggag gacccttggc taggatgggc cctaaaaaga
     2221 agcgtaaagc tagcatgaag ctgttgtctt caatggaaca agcttgcgac atatgccggt
     2281 taaaaaaact caggtgctcg aaggaaaagc ccaagtgtgc caagtgtctg aagaacaact
     2341 cggagtgccg ttattctccc aaggctatga ggtccccact gaccagggcg catctgacag
     2401 aggtagaatc aaggttggaa aaattggaag atttgtttct gctcatgttt cctcgggaaa
     2461 acctggacag cattttgaac atggactctt tggacgaagt gaaagtaatg ttgaaacaat
     2521 tatatatgca ggactatggt gacacggatg ctgctatgga tagcttgacc cctatggaaa
     2581 atggcatgtc tgaagggccg agaaaacaca tgaagactgc gacatcatcg ccaaaagtgg
     2641 atgaagatgg aagccaaagt cagttgagtg tatcgccgga attcccgctg cagtctggtg
     2701 gcggaggggc tcctaggggt tccggacggg ctgacgcatt ggacgatttt gatctggata
     2761 tgctgggaag tgacgccctc gatgattttg accttgacat gcttggttcg gatgcccttg
     2821 atgactttga cctcgacatg ctcggcagtg acgcccttga tgatttcgac ctggacatgc
     2881 tgattaacta aggtaccgaa ttcgctagcc ggccatacaa gtaatccgga tgatcgacgc
     2941 caacgtcgtt gaattttcaa attttaaata ctgaatattt gttttttttc ctattattta
     3001 tttattctct ttgtgttttt tttcttgctt tctaaaaaat taattcaatc caaatctaaa
     3061 catttttttt tctctttccg tctcccaatt cgtattccgc tcctctcatc tgaacacaat
     3121 gtgcaagttt atttatcttc tcgctttcat ttcattagga cgtgggggga attggtggaa
     3181 gggggaaaca cacaaaagga tgatggaaat gaaataagga cacacaatat gcaacaacat
     3241 tcaattcaga aatatggagg aaggtttaaa agaaaacata aaaatatata gaggaggaag
     3301 gaaaactagt aaaaaataag caaagaaatt aggcgaacga tgagaattgt cctcgcttgg
     3361 gcccaaaagg cctttttttt ttggcgccga cgtcaggtgg cacttttcgg ggaaatgtgc
     3421 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     3481 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     3541 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     3601 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     3661 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     3721 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     3781 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     3841 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     3901 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     3961 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     4021 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     4081 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     4141 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     4201 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     4261 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     4321 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     4381 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     4441 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     4501 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     4561 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     4621 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     4681 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     4741 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     4801 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     4861 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     4921 ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa
     4981 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     5041 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     5101 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     5161 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     5221 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     5281 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     5341 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     5401 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     5461 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     5521 aatttcacac aggaaacagc tatgaccatg attacgccaa gctgtaagtt taaacatgat
     5581 cttactaact aactattctc atttaaattt tcagagctta aaaatggctg aaatcactca
     5641 caacgatgga tacgctaaca acttggaaat gaaat
//
